Restriction map for
pF1KB5437
(Circular) (Six-base) MAPSORT of: pF1KB5437.seq Check: 4939 from: 1 to: 4112
pF1KB5437 4112 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 130 458 499 763 1417 2984 130
Size: 328 41 264 654 1567 1258
Fragments arranged by size:
1567 1258 654 328 264 41
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 610 1116 3465 3721 610
Size: 506 2349 256 1001
Fragments arranged by size:
2349 1001 506 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 1128 1542 1790 1825 1128
Size: 414 248 35 3415
Fragments arranged by size:
3415 414 248 35
BanII G_rGCy'C
Cuts at: 1126 2156 1126
Size: 1030 3082
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 575 1632 575
Size: 1057 3055
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4112
BglII A'GATC_T
Cuts at: 956 1627 3664 956
Size: 671 2037 1404
Fragments arranged by size:
2037 1404 671
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 1737 1830 2886 1737
Size: 93 1056 2963
Fragments arranged by size:
2963 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1288 2167 2659 2957 539
Size: 749 879 492 298 1694
Fragments arranged by size:
1694 879 749 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 217
Size: 4112
BseYI C'CCAG_C
Cuts at: 1537 2295 2872 3368 1537
Size: 758 577 496 2281
Fragments arranged by size:
2281 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 712 1126 1904 2094 2886 712
Size: 414 778 190 792 1938
Fragments arranged by size:
1938 792 778 414 190
Bsp1286I G_dGCh'C
Cuts at: 712 1126 1737 1830 1904 2094 2156 2886 712
Size: 414 611 93 74 190 62 730 1938
Fragments arranged by size:
1938 730 611 414 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 2110 2291 2500 2110
Size: 181 209 3722
Fragments arranged by size:
3722 209 181
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 130 1489 130
Size: 1359 2753
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstYI r'GATC_y
Cuts at: 1 956 1137 1604 1627 1962 2208 3220 3306
Size: 955 181 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 955 467 449 346 335 246 181
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 344 1355 344
Size: 1011 3101
DraI TTT'AAA
Cuts at: 717 938 1111 717
Size: 221 173 3718
Fragments arranged by size:
3718 221 173
DrdI GACnn_nn'nnGTC
Cuts at: 516 1818 2676 516
Size: 1302 858 1952
Fragments arranged by size:
1952 1302 858
EaeI y'GGCC_r
Cuts at: 1697 1871 2262 2289 3634 1697
Size: 174 391 27 1345 2175
Fragments arranged by size:
2175 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1937 2369 3116 3384 674
Size: 1263 432 747 268 1402
Fragments arranged by size:
1402 1263 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 183 1251 183
Size: 1068 3044
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 899 2783 2967 899
Size: 1884 184 2044
Fragments arranged by size:
2044 1884 184
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 349 2101 2228 349
Size: 1752 127 2233
Fragments arranged by size:
2233 1752 127
MspA1I CmG'CkG
Cuts at: 1223 1537 1897 2910 3155 1223
Size: 314 360 1013 245 2180
Fragments arranged by size:
2180 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NcoI C'CATG_G
Cuts at: 107 107
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PpuMI rG'GwC_Cy
Cuts at: 183 183
Size: 4112
PstI C_TGCA'G
Cuts at: 123 1159 1844 123
Size: 1036 685 2391
Fragments arranged by size:
2391 1036 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 722 3629 722
Size: 2907 1205
SfcI C'TryA_G
Cuts at: 33 119 1155 1840 2833 3024 33
Size: 86 1036 685 993 191 1121
Fragments arranged by size:
1121 1036 993 685 191 86
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 554 1267 2182 2674 2936 554
Size: 713 915 492 262 1730
Fragments arranged by size:
1730 915 713 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StyI C'CwwG_G
Cuts at: 107 1246 1600 107
Size: 1139 354 2619
Fragments arranged by size:
2619 1139 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1695 1828 2287 2496 1695
Size: 133 459 209 3311
Fragments arranged by size:
3311 459 209 133
TatI w'GTAC_w
Cuts at: 720 3627 720
Size: 2907 1205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 276 308 3629 3661 276
Size: 32 3321 32 727
Fragments arranged by size:
3321 727 32 32
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 321 1143 321
Size: 822 3290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 683 683
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI