Restriction map for
pF1KB5438
(Circular) (Six-base) MAPSORT of: pF1KB5438.seq Check: 3290 from: 1 to: 4439
pF1KB5438 4439 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 449 449
Size: 4439
AccI GT'mk_AC
Cuts at: 383 677 728 1477 383
Size: 294 51 749 3345
Fragments arranged by size:
3345 749 294 51
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 2264 2696 3049 3711 1438
Size: 826 432 353 662 2166
Fragments arranged by size:
2166 826 662 432 353
AflIII A'CryG_T
Cuts at: 1791 3591 3872 1791
Size: 1800 281 2358
Fragments arranged by size:
2358 1800 281
AleI CACnn'nnGTG
Cuts at: 508 508
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 939 1744 3182 939
Size: 805 1438 2196
Fragments arranged by size:
2196 1438 805
ApaI G_GGCC'C
Cuts at: 807 807
Size: 4439
ApaLI G'TGCA_C
Cuts at: 456 3277 456
Size: 2821 1618
ApoI r'AATT_y
Cuts at: 406 1443 3792 4048 406
Size: 1037 2349 256 797
Fragments arranged by size:
2349 1037 797 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 844 973 1459 844
Size: 129 486 3824
Fragments arranged by size:
3824 486 129
BamHI G'GATC_C
Cuts at: 111 1464 111
Size: 1353 3086
BanI G'GyrC_C
Cuts at: 1455 1869 2117 2152 1455
Size: 414 248 35 3742
Fragments arranged by size:
3742 414 248 35
BanII G_rGCy'C
Cuts at: 807 819 933 1453 2483 807
Size: 12 114 520 1030 2763
Fragments arranged by size:
2763 1030 520 114 12
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbsI GAAGACnn'nnnn_
Cuts at: 246 246
Size: 4439
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3393 4439 2337
Size: 1056 1046 2337
Fragments arranged by size:
2337 1056 1046
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglI GCCn_nnn'nGGC
Cuts at: 664 664
Size: 4439
BglII A'GATC_T
Cuts at: 737 1121 1954 3991 737
Size: 384 833 2037 1185
Fragments arranged by size:
2037 1185 833 384
BlpI GC'TnA_GC
Cuts at: 1551 1551
Size: 4439
Bme1580I G_kGCm'C
Cuts at: 460 707 807 2064 2157 3281 460
Size: 247 100 1257 93 1124 1618
Fragments arranged by size:
1618 1257 1124 247 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 889 1651 1732 2067 4211 889
Size: 762 81 335 2144 1117
Fragments arranged by size:
2144 1117 762 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 972 2806 972
Size: 1834 2605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 180 409 942 961 166
Size: 14 229 533 19 3644
Fragments arranged by size:
3644 533 229 19 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1615 2494 3208 3506 1153
Size: 462 879 714 298 2086
Fragments arranged by size:
2086 879 714 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 1051 4143 1051
Size: 3092 1347
BsaAI yAC'GTr
Cuts at: 702 1340 1792 702
Size: 638 452 3349
Fragments arranged by size:
3349 638 452
BsaBI GATnn'nnATC
Cuts at: 6 140 574 1953 3990 6
Size: 134 434 1379 2037 455
Fragments arranged by size:
2037 1379 455 434 134
BsaHI Gr'CG_yC
Cuts at: 446 759 1870 2118 2820 4316 446
Size: 313 1111 248 702 1496 569
Fragments arranged by size:
1496 1111 702 569 313 248
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3238 3385 3659 1628
Size: 521 678 411 147 274 2408
Fragments arranged by size:
2408 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 604 672 340
Size: 264 68 4107
Fragments arranged by size:
4107 264 68
BseYI C'CCAG_C
Cuts at: 1003 1196 1344 1864 2622 3287 3695 1003
Size: 193 148 520 758 665 408 1747
Fragments arranged by size:
1747 758 665 520 408 193 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 846
Size: 4439
BsiEI CG_ry'CG
Cuts at: 104 1981 2027 3257 104
Size: 1877 46 1230 1286
Fragments arranged by size:
1877 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 460 1453 2231 2421 3281 460
Size: 993 778 190 860 1618
Fragments arranged by size:
1618 993 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 696 696
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 460 707 807 819 933 1453 2064 2157 2231
Size: 247 100 12 114 520 611 93 74
Cuts at: 2231 2421 2483 3281 460
Size: 190 62 798 1618
Fragments arranged by size:
1618 798 611 520 247 190 114 100
93 74 62 12
BspHI T'CATG_A
Cuts at: 449 566 872 2871 449
Size: 117 306 1999 2017
Fragments arranged by size:
2017 1999 306 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1489 1703 2005 2386 1489
Size: 214 302 381 3542
Fragments arranged by size:
3542 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2731 2785 4269 4434 50
Size: 2681 54 1484 165 55
Fragments arranged by size:
2681 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 653 2351 653
Size: 1698 2741
BsrFI r'CCGG_y
Cuts at: 164 2437 2618 164
Size: 2273 181 1985
Fragments arranged by size:
2273 1985 181
BsrGI T'GTAC_A
Cuts at: 527 1325 527
Size: 798 3641
BssSI C'ACGA_G
Cuts at: 2710 3418 2710
Size: 708 3731
BstAPI GCAn_nnn'nTGC
Cuts at: 1816 1816
Size: 4439
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstEII G'GTnAC_C
Cuts at: 558 558
Size: 4439
BstYI r'GATC_y
Cuts at: 1 111 737 1121 1130 1421 1464 1931 1954
Size: 110 626 384 9 291 43 467 23
Cuts at: 1954 2289 2535 2841 2853 2939 3991 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 626 467 449 384 335 306 291
246 110 86 43 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 811 811
Size: 4439
BtgI C'CryG_G
Cuts at: 107 1041 107
Size: 934 3505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 225 2389 2570 2733 225
Size: 2164 181 163 1931
Fragments arranged by size:
2164 1931 181 163
BtsI GCAGTG_nn'
Cuts at: 223 344 1428 1682 223
Size: 121 1084 254 2980
Fragments arranged by size:
2980 1084 254 121
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 352 433 2145 3489 352
Size: 81 1712 1344 1302
Fragments arranged by size:
1712 1344 1302 81
EaeI y'GGCC_r
Cuts at: 341 512 2024 2198 2589 2616 3961 341
Size: 171 1512 174 391 27 1345 819
Fragments arranged by size:
1512 1345 819 391 174 171 27
EagI C'GGCC_G
Cuts at: 2024 2024
Size: 4439
EarI CTCTTCn'nnn_
Cuts at: 622 2462 2672 622
Size: 1840 210 2389
Fragments arranged by size:
2389 1840 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 208 921 2647 3389 3535 4249 208
Size: 713 1726 742 146 714 398
Fragments arranged by size:
1726 742 714 713 398 146
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 180 409 942 961 1438 2264 2696 3049
Size: 14 229 533 19 477 826 432 353
Cuts at: 3049 3711 166
Size: 662 894
Fragments arranged by size:
894 826 662 533 477 432 353 229
19 14
EcoO109I rG'GnC_Cy
Cuts at: 585 803 980 1578 585
Size: 218 177 598 3446
Fragments arranged by size:
3446 598 218 177
EcoRI G'AATT_C
Cuts at: 1443 1443
Size: 4439
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4439
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 301 555 1334 1364 1873 2121 3351 4122 301
Size: 254 779 30 509 248 1230 771 618
Fragments arranged by size:
1230 779 771 618 509 254 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2281 2311 2313 2343 2281
Size: 30 2 30 4377
Fragments arranged by size:
4377 30 30 2
HincII GTy'rAC
Cuts at: 774 1356 1478 774
Size: 582 122 3735
Fragments arranged by size:
3735 582 122
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3198 3382 3198
Size: 184 4255
MscI TGG'CCA
Cuts at: 514 2200 514
Size: 1686 2753
MslI CAynn'nnrTG
Cuts at: 280 508 520 565 1222 2428 2555 280
Size: 228 12 45 657 1206 127 2164
Fragments arranged by size:
2164 1206 657 228 127 45 12
MspA1I CmG'CkG
Cuts at: 508 1017 1550 1864 2224 3008 3253 508
Size: 509 533 314 360 784 245 1694
Fragments arranged by size:
1694 784 533 509 360 314 245
NaeI GCC'GGC
Cuts at: 166 2620 166
Size: 2454 1985
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NcoI C'CATG_G
Cuts at: 107 107
Size: 4439
NgoMIV G'CCGG_C
Cuts at: 164 2618 164
Size: 2454 1985
NspI r_CATG'y
Cuts at: 1096 1492 1992 3595 1096
Size: 396 500 1603 1940
Fragments arranged by size:
1940 1603 500 396
PasI CC'CwG_GG
Cuts at: 374 374
Size: 4439
PciI A'CATG_T
Cuts at: 3591 3591
Size: 4439
PflMI CCAn_nnn'nTGG
Cuts at: 581 939 581
Size: 358 4081
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PmlI CAC'GTG
Cuts at: 1340 1340
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 585 980 585
Size: 395 4044
PspOMI G'GGCC_C
Cuts at: 803 803
Size: 4439
PstI C_TGCA'G
Cuts at: 305 1486 2171 305
Size: 1181 685 2573
Fragments arranged by size:
2573 1181 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 508 1017 1864 2224 508
Size: 509 847 360 2723
Fragments arranged by size:
2723 847 509 360
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 301 540 1482 2167 3135 3326 33
Size: 268 239 942 685 968 191 1146
Fragments arranged by size:
1146 968 942 685 268 239 191
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 1168 1594 2509 3223 3485 1168
Size: 426 915 714 262 2122
Fragments arranged by size:
2122 915 714 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4439
SphI G_CATG'C
Cuts at: 1096 1492 1992 1096
Size: 396 500 3543
Fragments arranged by size:
3543 500 396
StuI AGG'CCT
Cuts at: 1411 1411
Size: 4439
StyI C'CwwG_G
Cuts at: 107 1573 1927 107
Size: 1466 354 2619
Fragments arranged by size:
2619 1466 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1017 2022 2155 2614 2823 1017
Size: 1005 133 459 209 2633
Fragments arranged by size:
2633 1005 459 209 133
TatI w'GTAC_w
Cuts at: 527 835 1325 3954 527
Size: 308 490 2629 1012
Fragments arranged by size:
2629 1012 490 308
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 408 440 3956 3988 408
Size: 32 3516 32 859
Fragments arranged by size:
3516 859 32 32
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1470 1470
Size: 4439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1934 1934
Size: 4439
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
ZraI GAC'GTC
Cuts at: 447 447
Size: 4439
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BspEI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI