Restriction map for
pF1KB5440
(Circular) (Six-base) MAPSORT of: pF1KB5440.seq Check: 2138 from: 1 to: 4094
pF1KB5440 4094 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 887 887
Size: 4094
AccI GT'mk_AC
Cuts at: 1132 1132
Size: 4094
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1919 2351 3098 3366 501
Size: 1418 432 747 268 1229
Fragments arranged by size:
1418 1229 747 432 268
AfeI AGC'GCT
Cuts at: 536 536
Size: 4094
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AhdI GACnn_n'nnGTC
Cuts at: 156 156
Size: 4094
AleI CACnn'nnGTG
Cuts at: 1074 1074
Size: 4094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 736
Size: 4094
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 781 813 781
Size: 32 4062
AlwNI CAG_nnn'CTG
Cuts at: 1399 2966 1399
Size: 1567 2527
ApaI G_GGCC'C
Cuts at: 319 382 653 1023 319
Size: 63 271 370 3390
Fragments arranged by size:
3390 370 271 63
ApaLI G'TGCA_C
Cuts at: 1077 2864 1077
Size: 1787 2307
ApoI r'AATT_y
Cuts at: 1098 3447 3703 1098
Size: 2349 256 1489
Fragments arranged by size:
2349 1489 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 281 312 843 1114 281
Size: 31 531 271 3261
Fragments arranged by size:
3261 531 271 31
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 402 482 1110 1524 1772 1807 402
Size: 80 628 414 248 35 2689
Fragments arranged by size:
2689 628 414 248 80 35
BanII G_rGCy'C
Cuts at: 319 382 431 653 677 827 1023 1108 2138
Size: 63 49 222 24 150 196 85 1030
Cuts at: 2138 319
Size: 2275
Fragments arranged by size:
2275 1030 222 196 150 85 63 49
24
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BbsI GAAGACnn'nnnn_
Cuts at: 192 371 429 192
Size: 179 58 3857
Fragments arranged by size:
3857 179 58
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 1609 3646 1609
Size: 2037 2057
BlpI GC'TnA_GC
Cuts at: 259 1206 259
Size: 947 3147
Bme1580I G_kGCm'C
Cuts at: 319 382 407 446 653 1023 1081 1719 1812
Size: 63 25 39 207 370 58 638 93
Cuts at: 1812 2868 319
Size: 1056 1545
Fragments arranged by size:
1545 1056 638 370 207 93 63 58
39 25
BmgBI CAC'GTC
Cuts at: 276 276
Size: 4094
BmrI ACTGGGnnnn_n'
Cuts at: 366 866 1306 1387 1722 3866 366
Size: 500 440 81 335 2144 594
Fragments arranged by size:
2144 594 500 440 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 841 2461 841
Size: 1620 2474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 811 577
Size: 234 3860
Bpu10I CC'TnA_GC
Cuts at: 350 463 350
Size: 113 3981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 301 3798 301
Size: 3497 597
BsaAI yAC'GTr
Cuts at: 495 660 1447 495
Size: 165 787 3142
Fragments arranged by size:
3142 787 165
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1525 1773 2475 3971 1525
Size: 248 702 1496 1648
Fragments arranged by size:
1648 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1283 1804 2482 2756 2903 3314 1283
Size: 521 678 274 147 411 2063
Fragments arranged by size:
2063 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 649
Size: 30 4064
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 634 439
Size: 195 3899
BseYI C'CCAG_C
Cuts at: 228 646 776 820 888 1519 2277 2854 3350
Size: 418 130 44 68 631 758 577 496
Cuts at: 3350 228
Size: 972
Fragments arranged by size:
972 758 631 577 496 418 130 68
44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 1011
Size: 4094
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2890 104
Size: 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 356 431 500 677 1081 1108 1886 2076 2868
Size: 75 69 177 404 27 778 190 792
Cuts at: 2868 356
Size: 1582
Fragments arranged by size:
1582 792 778 404 190 177 75 69
27
Bsp1286I G_dGCh'C
Cuts at: 319 356 382 407 431 446 500 653 677
Size: 37 26 25 24 15 54 153 24
Cuts at: 677 827 1023 1081 1108 1719 1812 1886 2076
Size: 150 196 58 27 611 93 74 190
Cuts at: 2076 2138 2868 319
Size: 62 730 1545
Fragments arranged by size:
1545 730 611 196 190 153 150 93
74 62 58 54 37 27 26 25
24 24 15
BspEI T'CCGG_A
Cuts at: 3314 3314
Size: 4094
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 146 611 887 1144 1358 1660 2041 146
Size: 465 276 257 214 302 381 2199
Fragments arranged by size:
2199 465 381 302 276 257 214
BsrBI CCG'CTC
Cuts at: 50 2386 2440 3924 4089 50
Size: 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 581 756 2006 581
Size: 175 1250 2669
Fragments arranged by size:
2669 1250 175
BsrFI r'CCGG_y
Cuts at: 740 803 2092 2273 2482 740
Size: 63 1289 181 209 2352
Fragments arranged by size:
2352 1289 209 181 63
BssSI C'ACGA_G
Cuts at: 2365 2723 2365
Size: 358 3736
BstAPI GCAn_nnn'nTGC
Cuts at: 1471 1471
Size: 4094
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstYI r'GATC_y
Cuts at: 1 476 1119 1586 1609 1944 2190 3202 3288
Size: 475 643 467 23 335 246 1012 86
Cuts at: 3288 3300 3646 1
Size: 12 346 449
Fragments arranged by size:
1012 643 475 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 641 641
Size: 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 903 2044 2225 2388 903
Size: 1141 181 163 2609
Fragments arranged by size:
2609 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 401 998 1337 401
Size: 597 339 3158
Fragments arranged by size:
3158 597 339
DraI TTT'AAA
Cuts at: 1093 1093
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 1679 1853 2244 2271 3616 1679
Size: 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 895 2117 2327 895
Size: 1222 210 2662
Fragments arranged by size:
2662 1222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 912 2302 2612 2758 3904 826
Size: 86 1390 310 146 1146 1016
Fragments arranged by size:
1390 1146 1016 310 146 86
EcoICRI GAG'CTC
Cuts at: 429 675 1106 429
Size: 246 431 3417
Fragments arranged by size:
3417 431 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 577 811 1919 2351 3098 3366 501
Size: 76 234 1108 432 747 268 1229
Fragments arranged by size:
1229 1108 747 432 268 234 76
EcoNI CCTnn'n_nnAGG
Cuts at: 120 120
Size: 4094
EcoO109I rG'GnC_Cy
Cuts at: 316 378 379 650 1019 1020 1233 316
Size: 62 1 271 369 1 213 3177
Fragments arranged by size:
3177 369 271 213 62 1 1
EcoRI G'AATT_C
Cuts at: 1098 1098
Size: 4094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 538 1528 1776 2798 3777 538
Size: 990 248 1022 979 855
Fragments arranged by size:
1022 990 979 855 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1936 1966 1968 1998 142
Size: 32 1762 30 2 30 2238
Fragments arranged by size:
2238 1762 32 30 30 2
HincII GTy'rAC
Cuts at: 600 1133 600
Size: 533 3561
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 785 1028 2765 2949 785
Size: 243 1737 184 1930
Fragments arranged by size:
1930 1737 243 184
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 493 544 586 881 1074 2083 2210 493
Size: 51 42 295 193 1009 127 2377
Fragments arranged by size:
2377 1009 295 193 127 51 42
MspA1I CmG'CkG
Cuts at: 138 263 1205 1519 1879 2892 3137 138
Size: 125 942 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 942 360 314 245 125
NaeI GCC'GGC
Cuts at: 2275 2275
Size: 4094
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NgoMIV G'CCGG_C
Cuts at: 2273 2273
Size: 4094
NspI r_CATG'y
Cuts at: 453 1147 1647 2554 453
Size: 694 500 907 1993
Fragments arranged by size:
1993 907 694 500
PasI CC'CwG_GG
Cuts at: 143 143
Size: 4094
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PflMI CCAn_nnn'nTGG
Cuts at: 390 1073 390
Size: 683 3411
PfoI T'CCnGG_A
Cuts at: 554 554
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PmlI CAC'GTG
Cuts at: 660 660
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PshAI GACnn'nnGTC
Cuts at: 703 703
Size: 4094
PspOMI G'GGCC_C
Cuts at: 315 378 649 1019 315
Size: 63 271 370 3390
Fragments arranged by size:
3390 370 271 63
PstI C_TGCA'G
Cuts at: 257 1141 1826 257
Size: 884 685 2525
Fragments arranged by size:
2525 884 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 138 263 1519 1879 138
Size: 125 1256 360 2353
Fragments arranged by size:
2353 1256 360 125
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 431 677 1108 431
Size: 246 431 3417
Fragments arranged by size:
3417 431 246
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SexAI A'CCwGG_T
Cuts at: 503 503
Size: 4094
SfcI C'TryA_G
Cuts at: 33 253 1137 1822 2815 3006 33
Size: 220 884 685 993 191 1121
Fragments arranged by size:
1121 993 884 685 220 191
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 283 1116 283
Size: 833 3261
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
SrfI GCCC'GGGC
Cuts at: 283 283
Size: 4094
StuI AGG'CCT
Cuts at: 965 965
Size: 4094
StyI C'CwwG_G
Cuts at: 1228 1582 1228
Size: 354 3740
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 649 681 3611 3643 649
Size: 32 2930 32 1100
Fragments arranged by size:
2930 1100 32 32
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 281 1114 281
Size: 833 3261
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PpuMI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI
ZraI