Restriction map for
pF1KB5447
(Circular) (Six-base) MAPSORT of: pF1KB5447.seq Check: 4720 from: 1 to: 4352
pF1KB5447 4352 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 866 2177 2609 3356 3624 165
Size: 701 1311 432 747 268 893
Fragments arranged by size:
1311 893 747 701 432 268
AflIII A'CryG_T
Cuts at: 1704 2808 3785 1704
Size: 1104 977 2271
Fragments arranged by size:
2271 1104 977
AgeI A'CCGG_T
Cuts at: 752 2740 752
Size: 1988 2364
AhdI GACnn_n'nnGTC
Cuts at: 576 576
Size: 4352
AleI CACnn'nnGTG
Cuts at: 680 680
Size: 4352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 305 305
Size: 4352
AlwNI CAG_nnn'CTG
Cuts at: 1657 3224 1657
Size: 1567 2785
ApaLI G'TGCA_C
Cuts at: 440 683 3122 440
Size: 243 2439 1670
Fragments arranged by size:
2439 1670 243
ApoI r'AATT_y
Cuts at: 1356 3705 3961 1356
Size: 2349 256 1747
Fragments arranged by size:
2349 1747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 1372 1372
Size: 4352
BamHI G'GATC_C
Cuts at: 189 1377 189
Size: 1188 3164
BanI G'GyrC_C
Cuts at: 111 1368 1782 2030 2065 111
Size: 1257 414 248 35 2398
Fragments arranged by size:
2398 1257 414 248 35
BanII G_rGCy'C
Cuts at: 896 1055 1366 2396 896
Size: 159 311 1030 2852
Fragments arranged by size:
2852 1030 311 159
BbeI G_GCGC'C
Cuts at: 1786 2034 1786
Size: 248 4104
BbsI GAAGACnn'nnnn_
Cuts at: 153 1087 153
Size: 934 3418
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3011 4352 2250
Size: 761 1341 2250
Fragments arranged by size:
2250 1341 761
BclI T'GATC_A
Cuts at: 1173 1872 1173
Size: 699 3653
BglII A'GATC_T
Cuts at: 1111 1867 3904 1111
Size: 756 2037 1559
Fragments arranged by size:
2037 1559 756
BlpI GC'TnA_GC
Cuts at: 1464 1464
Size: 4352
Bme1580I G_kGCm'C
Cuts at: 444 687 870 1128 1977 2070 3126 444
Size: 243 183 258 849 93 1056 1670
Fragments arranged by size:
1670 1056 849 258 243 183 93
BmrI ACTGGGnnnn_n'
Cuts at: 600 707 864 1208 1564 1645 1980 4124 600
Size: 107 157 344 356 81 335 2144 828
Fragments arranged by size:
2144 828 356 344 335 157 107 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 661 829 604
Size: 57 168 4127
Fragments arranged by size:
4127 168 57
Bpu10I CC'TnA_GC
Cuts at: 1049 1049
Size: 4352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 1528 2407 2899 3197 379
Size: 1149 879 492 298 1534
Fragments arranged by size:
1534 1149 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4056 4056
Size: 4352
BsaAI yAC'GTr
Cuts at: 1308 1705 1308
Size: 397 3955
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 1783 2031 2733 4229 1783
Size: 248 702 1496 1906
Fragments arranged by size:
1906 1496 702 248
BsaWI w'CCGG_w
Cuts at: 192 347 714 752 1094 1541 2062 2740 3014
Size: 155 367 38 342 447 521 678 274
Cuts at: 3014 3161 3572 192
Size: 147 411 972
Fragments arranged by size:
972 678 521 447 411 367 342 274
155 147 38
BseYI C'CCAG_C
Cuts at: 146 387 1777 2535 3112 3608 146
Size: 241 1390 758 577 496 890
Fragments arranged by size:
1390 890 758 577 496 241
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3148 104
Size: 1790 46 1208 1308
Fragments arranged by size:
1790 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 444 687 1020 1055 1366 2144 2334 3126 444
Size: 243 333 35 311 778 190 792 1670
Fragments arranged by size:
1670 792 778 333 311 243 190 35
BsmI GAATG_Cn'
Cuts at: 651 651
Size: 4352
Bsp1286I G_dGCh'C
Cuts at: 444 687 870 896 1020 1055 1128 1366 1977
Size: 243 183 26 124 35 73 238 611
Cuts at: 1977 2070 2144 2334 2396 3126 444
Size: 93 74 190 62 730 1670
Fragments arranged by size:
1670 730 611 243 238 190 183 124
93 74 73 62 35 26
BspEI T'CCGG_A
Cuts at: 192 347 714 3572 192
Size: 155 367 2858 972
Fragments arranged by size:
2858 972 367 155
BspHI T'CATG_A
Cuts at: 743 3528 743
Size: 2785 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1206 1259 1402 1616 1918 2299 1206
Size: 53 143 214 302 381 3259
Fragments arranged by size:
3259 381 302 214 143 53
BsrBI CCG'CTC
Cuts at: 50 2644 2698 4182 4347 50
Size: 2594 54 1484 165 55
Fragments arranged by size:
2594 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2264 2264
Size: 4352
BsrFI r'CCGG_y
Cuts at: 752 2350 2531 2740 752
Size: 1598 181 209 2364
Fragments arranged by size:
2364 1598 209 181
BssSI C'ACGA_G
Cuts at: 313 2623 2981 313
Size: 2310 358 1684
Fragments arranged by size:
2310 1684 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1729 1729
Size: 4352
BstBI TT'CG_AA
Cuts at: 447 2713 447
Size: 2266 2086
BstYI r'GATC_y
Cuts at: 1 189 1111 1377 1844 1867 2202 2448 3460
Size: 188 922 266 467 23 335 246 1012
Cuts at: 3460 3546 3558 3904 1
Size: 86 12 346 449
Fragments arranged by size:
1012 922 467 449 346 335 266 246
188 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 2483 2646 2302
Size: 181 163 4008
Fragments arranged by size:
4008 181 163
BtsI GCAGTG_nn'
Cuts at: 170 727 1595 170
Size: 557 868 2927
Fragments arranged by size:
2927 868 557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1260 1295 1260
Size: 35 4317
DraI TTT'AAA
Cuts at: 567 1351 567
Size: 784 3568
DrdI GACnn_nn'nnGTC
Cuts at: 2058 2916 2058
Size: 858 3494
EaeI y'GGCC_r
Cuts at: 1028 1310 1937 2111 2502 2529 3874 1028
Size: 282 627 174 391 27 1345 1506
Fragments arranged by size:
1506 1345 627 391 282 174 27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 598 2375 2585 598
Size: 1777 210 2365
Fragments arranged by size:
2365 1777 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2560 2870 3016 4162 2560
Size: 310 146 1146 2750
Fragments arranged by size:
2750 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1053 1364 1053
Size: 311 4041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 604 661 829 866 2177 2609 3356 3624
Size: 439 57 168 37 1311 432 747 268
Cuts at: 3624 165
Size: 893
Fragments arranged by size:
1311 893 747 439 432 268 168 57
37
EcoO109I rG'GnC_Cy
Cuts at: 1154 1491 1154
Size: 337 4015
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 1786 2034 3056 4035 1786
Size: 248 1022 979 2103
Fragments arranged by size:
2103 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 602 634 1103 1135 2194 2224 2226
Size: 32 8 32 469 32 1059 30 2
Cuts at: 2226 2256 562
Size: 30 2658
Fragments arranged by size:
2658 1059 469 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 210 1391 210
Size: 1181 3171
KasI G'GCGC_C
Cuts at: 1782 2030 1782
Size: 248 4104
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 909 3023 3207 909
Size: 2114 184 2054
Fragments arranged by size:
2114 2054 184
MscI TGG'CCA
Cuts at: 1030 1312 2113 1030
Size: 282 801 3269
Fragments arranged by size:
3269 801 282
MslI CAynn'nnrTG
Cuts at: 680 2341 2468 680
Size: 1661 127 2564
Fragments arranged by size:
2564 1661 127
MspA1I CmG'CkG
Cuts at: 556 1463 1777 2137 3150 3395 556
Size: 907 314 360 1013 245 1513
Fragments arranged by size:
1513 1013 907 360 314 245
NaeI GCC'GGC
Cuts at: 2533 2533
Size: 4352
NarI GG'CG_CC
Cuts at: 1783 2031 1783
Size: 248 4104
NcoI C'CATG_G
Cuts at: 107 107
Size: 4352
NgoMIV G'CCGG_C
Cuts at: 2531 2531
Size: 4352
NspI r_CATG'y
Cuts at: 1188 1405 1905 2812 1188
Size: 217 500 907 2728
Fragments arranged by size:
2728 907 500 217
PasI CC'CwG_GG
Cuts at: 251 1011 1150 251
Size: 760 139 3453
Fragments arranged by size:
3453 760 139
PciI A'CATG_T
Cuts at: 2808 2808
Size: 4352
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 4320
PpuMI rG'GwC_Cy
Cuts at: 1154 1154
Size: 4352
PstI C_TGCA'G
Cuts at: 1399 2084 1399
Size: 685 3667
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 1777 2137 1777
Size: 360 3992
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1055 1366 1055
Size: 311 4041
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SfcI C'TryA_G
Cuts at: 33 1395 2080 3073 3264 33
Size: 1362 685 993 191 1121
Fragments arranged by size:
1362 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1784 2032 1784
Size: 248 4104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 1374 1374
Size: 4352
SmlI C'TyrA_G
Cuts at: 394 1507 2422 2914 3176 394
Size: 1113 915 492 262 1570
Fragments arranged by size:
1570 1113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StyI C'CwwG_G
Cuts at: 107 372 1486 1840 107
Size: 265 1114 354 2619
Fragments arranged by size:
2619 1114 354 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 566 1935 2068 2527 2736 566
Size: 1369 133 459 209 2182
Fragments arranged by size:
2182 1369 459 209 133
TatI w'GTAC_w
Cuts at: 3867 3867
Size: 4352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1299 1383 1299
Size: 84 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1847 1847
Size: 4352
XmaI C'CCGG_G
Cuts at: 1372 1372
Size: 4352
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI