Restriction map for
pF1KB5455
(Circular) (Six-base) MAPSORT of: pF1KB5455.seq Check: 4536 from: 1 to: 4238
pF1KB5455 4238 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 137 1276 137
Size: 1139 3099
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 2063 2495 3242 3510 328
Size: 1735 432 747 268 1056
Fragments arranged by size:
1735 1056 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 1543 3110 1543
Size: 1567 2671
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 188 400 1258 188
Size: 212 858 3168
Fragments arranged by size:
3168 858 212
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 215 224 365 1012 1068 1254 1668 1916 1951
Size: 9 141 647 56 186 414 248 35
Cuts at: 1951 215
Size: 2502
Fragments arranged by size:
2502 647 414 248 186 141 56 35
9
BanII G_rGCy'C
Cuts at: 1252 2282 1252
Size: 1030 3208
BbeI G_GCGC'C
Cuts at: 219 1672 1920 219
Size: 1453 248 2537
Fragments arranged by size:
2537 1453 248
BbsI GAAGACnn'nnnn_
Cuts at: 189 189
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 629 1212 1246 595
Size: 34 583 34 3587
Fragments arranged by size:
3587 583 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 190 190
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 599 1863 1956 3012 599
Size: 1264 93 1056 1825
Fragments arranged by size:
1825 1264 1056 93
BmgBI CAC'GTC
Cuts at: 966 966
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 351 1450 1531 1866 4010 351
Size: 1099 81 335 2144 579
Fragments arranged by size:
2144 1099 579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 316 2605 316
Size: 2289 1949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 835
Size: 4238
Bpu10I CC'TnA_GC
Cuts at: 1106 1106
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 3942 475
Size: 3467 771
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1090 1174 1752 3789 6
Size: 1084 84 578 2037 455
Fragments arranged by size:
2037 1084 578 455 84
BsaHI Gr'CG_yC
Cuts at: 216 1669 1917 2619 4115 216
Size: 1453 248 702 1496 339
Fragments arranged by size:
1496 1453 702 339 248
BsaWI w'CCGG_w
Cuts at: 571 1427 1948 2626 2900 3047 3458 571
Size: 856 521 678 274 147 411 1351
Fragments arranged by size:
1351 856 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 739 769 130
Size: 30 579 30 3599
Fragments arranged by size:
3599 579 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 798 1091 418
Size: 380 293 3565
Fragments arranged by size:
3565 380 293
BseYI C'CCAG_C
Cuts at: 495 1072 1663 2421 2998 3494 495
Size: 577 591 758 577 496 1239
Fragments arranged by size:
1239 758 591 577 577 496
BsiEI CG_ry'CG
Cuts at: 104 719 1780 1826 3034 104
Size: 615 1061 46 1208 1308
Fragments arranged by size:
1308 1208 1061 615 46
BsiHKAI G_wGCw'C
Cuts at: 326 410 1252 2030 2220 3012 326
Size: 84 842 778 190 792 1552
Fragments arranged by size:
1552 842 792 778 190 84
Bsp1286I G_dGCh'C
Cuts at: 326 410 599 1252 1863 1956 2030 2220 2282
Size: 84 189 653 611 93 74 190 62
Cuts at: 2282 3012 326
Size: 730 1552
Fragments arranged by size:
1552 730 653 611 190 189 93 84
74 62
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 674 953 3414 674
Size: 279 2461 1498
Fragments arranged by size:
2461 1498 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1502 1804 2185 1288
Size: 214 302 381 3341
Fragments arranged by size:
3341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 723 867 2530 2584 4068 4233 50
Size: 673 144 1663 54 1484 165 55
Fragments arranged by size:
1663 1484 673 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 867 1031 1094 2150 867
Size: 164 63 1056 2955
Fragments arranged by size:
2955 1056 164 63
BsrFI r'CCGG_y
Cuts at: 163 2236 2417 2626 163
Size: 2073 181 209 1775
Fragments arranged by size:
2073 1775 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 414 1615 414
Size: 1201 3037
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstEII G'GTnAC_C
Cuts at: 582 582
Size: 4238
BstYI r'GATC_y
Cuts at: 1 680 1263 1730 1753 2088 2334 3346 3432
Size: 679 583 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1012 679 583 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 881 881
Size: 4238
BtgI C'CryG_G
Cuts at: 107 713 791 107
Size: 606 78 3554
Fragments arranged by size:
3554 606 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1058 1481 1058
Size: 423 3815
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DraIII CAC_nnn'GTG
Cuts at: 414 594 414
Size: 180 4058
DrdI GACnn_nn'nnGTC
Cuts at: 844 1944 2802 844
Size: 1100 858 2280
Fragments arranged by size:
2280 1100 858
EaeI y'GGCC_r
Cuts at: 543 651 716 788 840 1823 1997 2388 2415
Size: 108 65 72 52 983 174 391 27
Cuts at: 2415 3760 543
Size: 1345 1021
Fragments arranged by size:
1345 1021 983 391 174 108 72 65
52 27
EagI C'GGCC_G
Cuts at: 716 1823 716
Size: 1107 3131
EarI CTCTTCn'nnn_
Cuts at: 814 895 2261 2471 814
Size: 81 1366 210 2581
Fragments arranged by size:
2581 1366 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 835 2063 2495 3242 3510 328
Size: 507 1228 432 747 268 1056
Fragments arranged by size:
1228 1056 747 507 432 268
EcoO109I rG'GnC_Cy
Cuts at: 429 657 1377 429
Size: 228 720 3290
Fragments arranged by size:
3290 720 228
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
EcoRV GAT'ATC
Cuts at: 120 120
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 2257 183
Size: 2074 2164
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 219 1672 1920 2942 3921 219
Size: 1453 248 1022 979 536
Fragments arranged by size:
1453 1022 979 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 1083 1115 2080 2110 2112 2142 130
Size: 32 921 32 965 30 2 30 2226
Fragments arranged by size:
2226 965 921 32 32 30 30 2
HincII GTy'rAC
Cuts at: 138 1277 138
Size: 1139 3099
KasI G'GCGC_C
Cuts at: 215 1668 1916 215
Size: 1453 248 2537
Fragments arranged by size:
2537 1453 248
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 790 1999 790
Size: 1209 3029
MslI CAynn'nnrTG
Cuts at: 562 1018 2227 2354 562
Size: 456 1209 127 2446
Fragments arranged by size:
2446 1209 456 127
MspA1I CmG'CkG
Cuts at: 875 1349 1663 2023 3036 3281 875
Size: 474 314 360 1013 245 1832
Fragments arranged by size:
1832 1013 474 360 314 245
NaeI GCC'GGC
Cuts at: 165 2419 165
Size: 2254 1984
NarI GG'CG_CC
Cuts at: 216 1669 1917 216
Size: 1453 248 2537
Fragments arranged by size:
2537 1453 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 163 2417 163
Size: 2254 1984
NspI r_CATG'y
Cuts at: 156 1291 1791 2698 156
Size: 1135 500 907 1696
Fragments arranged by size:
1696 1135 907 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PpuMI rG'GwC_Cy
Cuts at: 657 657
Size: 4238
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 136 1275 136
Size: 1139 3099
SapI GCTCTTCn'nnn_
Cuts at: 814 2261 2471 814
Size: 1447 210 2581
Fragments arranged by size:
2581 1447 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 1117 3755 1117
Size: 2638 1600
SfcI C'TryA_G
Cuts at: 33 699 1281 1966 2959 3150 33
Size: 666 582 685 993 191 1121
Fragments arranged by size:
1121 993 685 666 582 191
SfoI GGC'GCC
Cuts at: 217 1670 1918 217
Size: 1453 248 2537
Fragments arranged by size:
2537 1453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 190 1260 190
Size: 1070 3168
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 107 443 1372 1726 107
Size: 336 929 354 2619
Fragments arranged by size:
2619 929 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 439 601 1086 1821 1954 2413 2622 439
Size: 162 485 735 133 459 209 2055
Fragments arranged by size:
2055 735 485 459 209 162 133
TatI w'GTAC_w
Cuts at: 1115 3753 1115
Size: 2638 1600
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 739 771 3755 3787 739
Size: 32 2984 32 1190
Fragments arranged by size:
2984 1190 32 32
Tth111I GACn'n_nGTC
Cuts at: 580 2035 580
Size: 1455 2783
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 636 810 1733 636
Size: 174 923 3141
Fragments arranged by size:
3141 923 174
XmaI C'CCGG_G
Cuts at: 188 1258 188
Size: 1070 3168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI