Restriction map for
pF1KB5463
(Circular) (Six-base) MAPSORT of: pF1KB5463.seq Check: 144 from: 1 to: 3977
pF1KB5463 3977 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 204 204
Size: 3977
AccI GT'mk_AC
Cuts at: 830 1015 830
Size: 185 3792
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1802 2234 2981 3249 1802
Size: 432 747 268 2530
Fragments arranged by size:
2530 747 432 268
AfeI AGC'GCT
Cuts at: 538 538
Size: 3977
AflIII A'CryG_T
Cuts at: 749 1329 2433 3410 749
Size: 580 1104 977 1316
Fragments arranged by size:
1316 1104 977 580
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 418
Size: 32 3945
AlwNI CAG_nnn'CTG
Cuts at: 681 1282 2849 681
Size: 601 1567 1809
Fragments arranged by size:
1809 1567 601
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AscI GG'CGCG_CC
Cuts at: 889 889
Size: 3977
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 997 997
Size: 3977
BamHI G'GATC_C
Cuts at: 449 1002 449
Size: 553 3424
BanI G'GyrC_C
Cuts at: 993 1407 1655 1690 993
Size: 414 248 35 3280
Fragments arranged by size:
3280 414 248 35
BanII G_rGCy'C
Cuts at: 131 991 2021 131
Size: 860 1030 2087
Fragments arranged by size:
2087 1030 860
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 322 364 463 322
Size: 42 99 3836
Fragments arranged by size:
3836 99 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 924 958 924
Size: 34 3943
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 908 1497 908
Size: 589 3388
BglI GCCn_nnn'nGGC
Cuts at: 292 292
Size: 3977
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 1602 1695 2751 1602
Size: 93 1056 2828
Fragments arranged by size:
2828 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1189 1270 1605 3749 1189
Size: 81 335 2144 1417
Fragments arranged by size:
2144 1417 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 3977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 622 634 645 742 586
Size: 36 12 11 97 3821
Fragments arranged by size:
3821 97 36 12 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 179 3681 179
Size: 3502 475
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 913 1491 3528 6
Size: 907 578 2037 455
Fragments arranged by size:
2037 907 578 455
BsaHI Gr'CG_yC
Cuts at: 1408 1656 2358 3854 1408
Size: 248 702 1496 1531
Fragments arranged by size:
1531 1496 702 248
BsaWI w'CCGG_w
Cuts at: 452 1166 1687 2365 2639 2786 3197 452
Size: 714 521 678 274 147 411 1232
Fragments arranged by size:
1232 714 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 484 514 418
Size: 30 36 30 3881
Fragments arranged by size:
3881 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 277 340 423 610 235
Size: 42 63 83 187 3602
Fragments arranged by size:
3602 187 83 63 42
BseYI C'CCAG_C
Cuts at: 1402 2160 2737 3233 1402
Size: 758 577 496 2146
Fragments arranged by size:
2146 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 991 1769 1959 2751 131
Size: 860 778 190 792 1357
Fragments arranged by size:
1357 860 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 688 688
Size: 3977
Bsp1286I G_dGCh'C
Cuts at: 131 991 1602 1695 1769 1959 2021 2751 131
Size: 860 611 93 74 190 62 730 1357
Fragments arranged by size:
1357 860 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 452 3197 452
Size: 2745 1232
BspHI T'CATG_A
Cuts at: 905 3153 905
Size: 2248 1729
BspMI ACCTGCnnnn'nnnn_
Cuts at: 204 1027 1241 1543 1924 204
Size: 823 214 302 381 2257
Fragments arranged by size:
2257 823 381 302 214
BsrBI CCG'CTC
Cuts at: 50 431 2269 2323 3807 3972 50
Size: 381 1838 54 1484 165 55
Fragments arranged by size:
1838 1484 381 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1889 1889
Size: 3977
BsrFI r'CCGG_y
Cuts at: 574 1975 2156 2365 574
Size: 1401 181 209 2186
Fragments arranged by size:
2186 1401 209 181
BssHII G'CGCG_C
Cuts at: 889 889
Size: 3977
BssSI C'ACGA_G
Cuts at: 772 2248 2606 772
Size: 1476 358 2143
Fragments arranged by size:
2143 1476 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstXI CCAn_nnnn'nTGG
Cuts at: 774 774
Size: 3977
BstYI r'GATC_y
Cuts at: 1 449 1002 1469 1492 1827 2073 3085 3171
Size: 448 553 467 23 335 246 1012 86
Cuts at: 3171 3183 3529 1
Size: 12 346 449
Fragments arranged by size:
1012 553 467 449 448 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 445 445
Size: 3977
BtgI C'CryG_G
Cuts at: 176 176
Size: 3977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 164 927 1927 2108 2271 164
Size: 763 1000 181 163 1870
Fragments arranged by size:
1870 1000 763 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 137 764 1562 1736 2127 2154 3499 137
Size: 627 798 174 391 27 1345 615
Fragments arranged by size:
1345 798 627 615 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 129 989 129
Size: 860 3117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 622 634 645 742 1802 2234 2981 3249
Size: 36 12 11 97 1060 432 747 268
Cuts at: 3249 586
Size: 1314
Fragments arranged by size:
1314 1060 747 432 268 97 36 12
11
EcoO109I rG'GnC_Cy
Cuts at: 1116 1116
Size: 3977
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 540 1411 1659 2681 3660 540
Size: 871 248 1022 979 857
Fragments arranged by size:
1022 979 871 857 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 1819 1849 1851 1881 484
Size: 32 1303 30 2 30 2580
Fragments arranged by size:
2580 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 482 548 2648 2832 371
Size: 111 66 2100 184 1516
Fragments arranged by size:
2100 1516 184 111 66
MscI TGG'CCA
Cuts at: 766 1738 766
Size: 972 3005
MslI CAynn'nnrTG
Cuts at: 754 760 766 772 1966 2093 754
Size: 6 6 6 1194 127 2638
Fragments arranged by size:
2638 1194 127 6 6 6
MspA1I CmG'CkG
Cuts at: 439 564 1088 1402 1762 2775 3020 439
Size: 125 524 314 360 1013 245 1396
Fragments arranged by size:
1396 1013 524 360 314 245 125
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NspI r_CATG'y
Cuts at: 612 753 1030 1530 2437 612
Size: 141 277 500 907 2152
Fragments arranged by size:
2152 907 500 277 141
PciI A'CATG_T
Cuts at: 749 2433 749
Size: 1684 2293
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 418 450 3116 3148 418
Size: 32 2666 32 1247
Fragments arranged by size:
2666 1247 32 32
PstI C_TGCA'G
Cuts at: 1024 1709 1024
Size: 685 3292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 564 1402 1762 564
Size: 838 360 2779
Fragments arranged by size:
2779 838 360
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 131 991 131
Size: 860 3117
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SfcI C'TryA_G
Cuts at: 33 1020 1705 2698 2889 33
Size: 987 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 191
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StuI AGG'CCT
Cuts at: 422 422
Size: 3977
StyI C'CwwG_G
Cuts at: 140 1111 1465 140
Size: 971 354 2652
Fragments arranged by size:
2652 971 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 197 1560 1693 2152 2361 131
Size: 66 1363 133 459 209 1747
Fragments arranged by size:
1747 1363 459 209 133 66
TatI w'GTAC_w
Cuts at: 506 809 3492 506
Size: 303 2683 991
Fragments arranged by size:
2683 991 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 267 299 3494 3526 267
Size: 32 3195 32 718
Fragments arranged by size:
3195 718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 597 1472 486
Size: 111 875 2991
Fragments arranged by size:
2991 875 111
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI ApaI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI
BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI