Restriction map for
pF1KB5464
(Circular) (Six-base) MAPSORT of: pF1KB5464.seq Check: 5380 from: 1 to: 3911
pF1KB5464 3911 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 295 295
Size: 3911
AccI GT'mk_AC
Cuts at: 949 949
Size: 3911
Acc65I G'GTAC_C
Cuts at: 240 927 240
Size: 687 3224
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2168 2915 3183 1736
Size: 432 747 268 2464
Fragments arranged by size:
2464 747 432 268
AflIII A'CryG_T
Cuts at: 362 1263 2367 3344 362
Size: 901 1104 977 929
Fragments arranged by size:
1104 977 929 901
AgeI A'CCGG_T
Cuts at: 2299 2299
Size: 3911
AlwNI CAG_nnn'CTG
Cuts at: 1216 2783 1216
Size: 1567 2344
ApaLI G'TGCA_C
Cuts at: 2681 2681
Size: 3911
ApoI r'AATT_y
Cuts at: 490 877 915 3264 3520 490
Size: 387 38 2349 256 881
Fragments arranged by size:
2349 881 387 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 931 931
Size: 3911
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 240 927 1341 1589 1624 240
Size: 687 414 248 35 2527
Fragments arranged by size:
2527 687 414 248 35
BanII G_rGCy'C
Cuts at: 167 416 925 1955 167
Size: 249 509 1030 2123
Fragments arranged by size:
2123 1030 509 249
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 834 868 834
Size: 34 3877
BciVI GTATCCnnnnn_n'
Cuts at: 1809 2570 3911 1809
Size: 761 1341 1809
Fragments arranged by size:
1809 1341 761
BclI T'GATC_A
Cuts at: 744 1431 744
Size: 687 3224
BglII A'GATC_T
Cuts at: 579 1426 3463 579
Size: 847 2037 1027
Fragments arranged by size:
2037 1027 847
BlpI GC'TnA_GC
Cuts at: 1023 1023
Size: 3911
Bme1580I G_kGCm'C
Cuts at: 1536 1629 2685 1536
Size: 93 1056 2762
Fragments arranged by size:
2762 1056 93
BmgBI CAC'GTC
Cuts at: 363 363
Size: 3911
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1204 1539 3683 1123
Size: 81 335 2144 1351
Fragments arranged by size:
2144 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1966 2458 2756 1087
Size: 879 492 298 2242
Fragments arranged by size:
2242 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3615 3615
Size: 3911
BsaAI yAC'GTr
Cuts at: 1264 1264
Size: 3911
BsaBI GATnn'nnATC
Cuts at: 6 527 1425 3462 6
Size: 521 898 2037 455
Fragments arranged by size:
2037 898 521 455
BsaHI Gr'CG_yC
Cuts at: 203 1342 1590 2292 3788 203
Size: 1139 248 702 1496 326
Fragments arranged by size:
1496 1139 702 326 248
BsaWI w'CCGG_w
Cuts at: 1100 1621 2299 2573 2720 3131 1100
Size: 521 678 274 147 411 1880
Fragments arranged by size:
1880 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 335 193
Size: 142 3769
BseYI C'CCAG_C
Cuts at: 429 1336 2094 2671 3167 429
Size: 907 758 577 496 1173
Fragments arranged by size:
1173 907 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2707 104
Size: 1349 46 1208 1308
Fragments arranged by size:
1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 713 925 1703 1893 2685 713
Size: 212 778 190 792 1939
Fragments arranged by size:
1939 792 778 212 190
Bsp1286I G_dGCh'C
Cuts at: 167 416 713 925 1536 1629 1703 1893 1955
Size: 249 297 212 611 93 74 190 62
Cuts at: 1955 2685 167
Size: 730 1393
Fragments arranged by size:
1393 730 611 297 249 212 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3131 3131
Size: 3911
BspHI T'CATG_A
Cuts at: 546 705 777 3087 546
Size: 159 72 2310 1370
Fragments arranged by size:
2310 1370 159 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 961 1175 1477 1858 295
Size: 666 214 302 381 2348
Fragments arranged by size:
2348 666 381 302 214
BsrBI CCG'CTC
Cuts at: 50 133 173 2203 2257 3741 3906 50
Size: 83 40 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 83 55 54 40
BsrDI GCAATG_nn'
Cuts at: 1823 1823
Size: 3911
BsrFI r'CCGG_y
Cuts at: 1909 2090 2299 1909
Size: 181 209 3521
Fragments arranged by size:
3521 209 181
BsrGI T'GTAC_A
Cuts at: 332 332
Size: 3911
BssSI C'ACGA_G
Cuts at: 2182 2540 2182
Size: 358 3553
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1288
Size: 3911
BstBI TT'CG_AA
Cuts at: 2272 2272
Size: 3911
BstYI r'GATC_y
Cuts at: 1 579 936 1403 1426 1761 2007 3019 3105
Size: 578 357 467 23 335 246 1012 86
Cuts at: 3105 3117 3463 1
Size: 12 346 449
Fragments arranged by size:
1012 578 467 449 357 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 884 107
Size: 777 3134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 2042 2205 1861
Size: 181 163 3567
Fragments arranged by size:
3567 181 163
BtsI GCAGTG_nn'
Cuts at: 1154 1154
Size: 3911
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2475 1617
Size: 858 3053
EaeI y'GGCC_r
Cuts at: 249 1496 1670 2061 2088 3433 249
Size: 1247 174 391 27 1345 727
Fragments arranged by size:
1345 1247 727 391 174 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 252 1934 2144 252
Size: 1682 210 2019
Fragments arranged by size:
2019 1682 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2119 2429 2575 3721 2119
Size: 310 146 1146 2309
Fragments arranged by size:
2309 1146 310 146
EcoICRI GAG'CTC
Cuts at: 923 923
Size: 3911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1736 2168 2915 3183 670
Size: 1066 432 747 268 1398
Fragments arranged by size:
1398 1066 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 225 1050 225
Size: 825 3086
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 1692 1692
Size: 3911
HaeII r_GCGC'y
Cuts at: 187 1345 1593 2615 3594 187
Size: 1158 248 1022 979 504
Fragments arranged by size:
1158 1022 979 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1753 1783 1785 1815 1753
Size: 30 2 30 3849
Fragments arranged by size:
3849 30 30 2
HincII GTy'rAC
Cuts at: 950 950
Size: 3911
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 244 931 244
Size: 687 3224
MfeI C'AATT_G
Cuts at: 449 846 449
Size: 397 3514
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2582 2766 2582
Size: 184 3727
MscI TGG'CCA
Cuts at: 251 1672 251
Size: 1421 2490
MslI CAynn'nnrTG
Cuts at: 628 1900 2027 628
Size: 1272 127 2512
Fragments arranged by size:
2512 1272 127
MspA1I CmG'CkG
Cuts at: 433 1022 1336 1696 2709 2954 433
Size: 589 314 360 1013 245 1390
Fragments arranged by size:
1390 1013 589 360 314 245
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NcoI C'CATG_G
Cuts at: 107 107
Size: 3911
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NspI r_CATG'y
Cuts at: 279 964 1464 2371 279
Size: 685 500 907 1819
Fragments arranged by size:
1819 907 685 500
PciI A'CATG_T
Cuts at: 2367 2367
Size: 3911
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 3879
PpuMI rG'GwC_Cy
Cuts at: 225 225
Size: 3911
PstI C_TGCA'G
Cuts at: 423 958 1643 423
Size: 535 685 2691
Fragments arranged by size:
2691 685 535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 433 1336 1696 433
Size: 903 360 2648
Fragments arranged by size:
2648 903 360
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 925 925
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 252 1934 2144 252
Size: 1682 210 2019
Fragments arranged by size:
2019 1682 210
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 3428 3428
Size: 3911
SfcI C'TryA_G
Cuts at: 33 419 954 1639 2632 2823 33
Size: 386 535 685 993 191 1121
Fragments arranged by size:
1121 993 685 535 386 191
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 1066 1981 2473 2735 1066
Size: 915 492 262 2242
Fragments arranged by size:
2242 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 964 1464 964
Size: 500 3411
SspI AAT'ATT
Cuts at: 340 462 340
Size: 122 3789
StyI C'CwwG_G
Cuts at: 107 1045 1399 107
Size: 938 354 2619
Fragments arranged by size:
2619 938 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 651 1494 1627 2086 2295 651
Size: 843 133 459 209 2267
Fragments arranged by size:
2267 843 459 209 133
TatI w'GTAC_w
Cuts at: 332 365 383 3426 332
Size: 33 18 3043 817
Fragments arranged by size:
3043 817 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 205 773 1708 205
Size: 568 935 2408
Fragments arranged by size:
2408 935 568
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 645 1406 645
Size: 761 3150
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI