Restriction map for
pF1KB5469
(Circular) (Six-base) MAPSORT of: pF1KB5469.seq Check: 8547 from: 1 to: 3587
pF1KB5469 3587 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 233 625 233
Size: 392 3195
Acc65I G'GTAC_C
Cuts at: 603 603
Size: 3587
AclI AA'CG_TT
Cuts at: 3427 3427
Size: 3587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1412 1844 2591 2859 1412
Size: 432 747 268 2140
Fragments arranged by size:
2140 747 432 268
AflIII A'CryG_T
Cuts at: 939 2043 3020 939
Size: 1104 977 1506
Fragments arranged by size:
1506 1104 977
AgeI A'CCGG_T
Cuts at: 1975 1975
Size: 3587
AlwNI CAG_nnn'CTG
Cuts at: 554 892 2459 554
Size: 338 1567 1682
Fragments arranged by size:
1682 1567 338
ApaLI G'TGCA_C
Cuts at: 2357 2357
Size: 3587
ApoI r'AATT_y
Cuts at: 591 2940 3196 591
Size: 2349 256 982
Fragments arranged by size:
2349 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
AvaI C'yCGr_G
Cuts at: 607 607
Size: 3587
BamHI G'GATC_C
Cuts at: 612 612
Size: 3587
BanI G'GyrC_C
Cuts at: 603 1017 1265 1300 603
Size: 414 248 35 2890
Fragments arranged by size:
2890 414 248 35
BanII G_rGCy'C
Cuts at: 601 1631 601
Size: 1030 2557
BbeI G_GCGC'C
Cuts at: 1021 1269 1021
Size: 248 3339
BciVI GTATCCnnnnn_n'
Cuts at: 1485 2246 3587 1485
Size: 761 1341 1485
Fragments arranged by size:
1485 1341 761
BclI T'GATC_A
Cuts at: 1107 1107
Size: 3587
BglII A'GATC_T
Cuts at: 1102 3139 1102
Size: 2037 1550
BlpI GC'TnA_GC
Cuts at: 699 699
Size: 3587
Bme1580I G_kGCm'C
Cuts at: 1212 1305 2361 1212
Size: 93 1056 2438
Fragments arranged by size:
2438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 799 880 1215 3359 799
Size: 81 335 2144 1027
Fragments arranged by size:
2144 1027 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1954 1954
Size: 3587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1642 2134 2432 763
Size: 879 492 298 1918
Fragments arranged by size:
1918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3291 3291
Size: 3587
BsaAI yAC'GTr
Cuts at: 940 940
Size: 3587
BsaBI GATnn'nnATC
Cuts at: 6 1101 3138 6
Size: 1095 2037 455
Fragments arranged by size:
2037 1095 455
BsaHI Gr'CG_yC
Cuts at: 1018 1266 1968 3464 1018
Size: 248 702 1496 1141
Fragments arranged by size:
1496 1141 702 248
BsaWI w'CCGG_w
Cuts at: 776 1297 1975 2249 2396 2807 776
Size: 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1012 1770 2347 2843 1012
Size: 758 577 496 1756
Fragments arranged by size:
1756 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1129 1175 2383 104
Size: 1025 46 1208 1308
Fragments arranged by size:
1308 1208 1025 46
BsiHKAI G_wGCw'C
Cuts at: 601 1379 1569 2361 601
Size: 778 190 792 1827
Fragments arranged by size:
1827 792 778 190
BsmI GAATG_Cn'
Cuts at: 311 311
Size: 3587
BsmBI CGTCTCn'nnnn_
Cuts at: 520 520
Size: 3587
Bsp1286I G_dGCh'C
Cuts at: 601 1212 1305 1379 1569 1631 2361 601
Size: 611 93 74 190 62 730 1827
Fragments arranged by size:
1827 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2807 2807
Size: 3587
BspHI T'CATG_A
Cuts at: 2763 2763
Size: 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 637 851 1153 1534 442
Size: 195 214 302 381 2495
Fragments arranged by size:
2495 381 302 214 195
BsrBI CCG'CTC
Cuts at: 50 1879 1933 3417 3582 50
Size: 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 170 1499 170
Size: 1329 2258
BsrFI r'CCGG_y
Cuts at: 1585 1766 1975 1585
Size: 181 209 3197
Fragments arranged by size:
3197 209 181
BssSI C'ACGA_G
Cuts at: 1858 2216 1858
Size: 358 3229
BstAPI GCAn_nnn'nTGC
Cuts at: 964 964
Size: 3587
BstBI TT'CG_AA
Cuts at: 1948 1948
Size: 3587
BstYI r'GATC_y
Cuts at: 1 495 612 1079 1102 1437 1683 2695 2781
Size: 494 117 467 23 335 246 1012 86
Cuts at: 2781 2793 3139 1
Size: 12 346 449
Fragments arranged by size:
1012 494 467 449 346 335 246 117
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 1718 1881 1537
Size: 181 163 3243
Fragments arranged by size:
3243 181 163
BtsI GCAGTG_nn'
Cuts at: 547 830 547
Size: 283 3304
DraI TTT'AAA
Cuts at: 586 586
Size: 3587
DrdI GACnn_nn'nnGTC
Cuts at: 1293 2151 1293
Size: 858 2729
EaeI y'GGCC_r
Cuts at: 1172 1346 1737 1764 3109 1172
Size: 174 391 27 1345 1650
Fragments arranged by size:
1650 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1172 1172
Size: 3587
EarI CTCTTCn'nnn_
Cuts at: 295 1610 1820 295
Size: 1315 210 2062
Fragments arranged by size:
2062 1315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1795 2105 2251 3397 127
Size: 1668 310 146 1146 317
Fragments arranged by size:
1668 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 599 599
Size: 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1412 1844 2591 2859 1412
Size: 432 747 268 2140
Fragments arranged by size:
2140 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 726 726
Size: 3587
EcoRI G'AATT_C
Cuts at: 591 591
Size: 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 1606
Size: 3587
FspI TGC'GCA
Cuts at: 1368 1368
Size: 3587
HaeII r_GCGC'y
Cuts at: 1021 1269 2291 3270 1021
Size: 248 1022 979 1338
Fragments arranged by size:
1338 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 453 485 1429 1459 1461 1491 453
Size: 32 944 30 2 30 2549
Fragments arranged by size:
2549 944 32 30 30 2
HincII GTy'rAC
Cuts at: 626 626
Size: 3587
KasI G'GCGC_C
Cuts at: 1017 1265 1017
Size: 248 3339
KpnI G_GTAC'C
Cuts at: 607 607
Size: 3587
MluI A'CGCG_T
Cuts at: 3020 3020
Size: 3587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2442 2258
Size: 184 3403
MscI TGG'CCA
Cuts at: 1348 1348
Size: 3587
MslI CAynn'nnrTG
Cuts at: 316 1576 1703 316
Size: 1260 127 2200
Fragments arranged by size:
2200 1260 127
MspA1I CmG'CkG
Cuts at: 698 1012 1372 2385 2630 698
Size: 314 360 1013 245 1655
Fragments arranged by size:
1655 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1768 1768
Size: 3587
NarI GG'CG_CC
Cuts at: 1018 1266 1018
Size: 248 3339
NcoI C'CATG_G
Cuts at: 107 107
Size: 3587
NgoMIV G'CCGG_C
Cuts at: 1766 1766
Size: 3587
NspI r_CATG'y
Cuts at: 640 1140 2047 640
Size: 500 907 2180
Fragments arranged by size:
2180 907 500
PciI A'CATG_T
Cuts at: 2043 2043
Size: 3587
PmeI GTTT'AAAC
Cuts at: 586 586
Size: 3587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 3555
PstI C_TGCA'G
Cuts at: 559 634 1319 559
Size: 75 685 2827
Fragments arranged by size:
2827 685 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3587
PvuII CAG'CTG
Cuts at: 1012 1372 1012
Size: 360 3227
RsrII CG'GwC_CG
Cuts at: 1782 1782
Size: 3587
SacI G_AGCT'C
Cuts at: 601 601
Size: 3587
SalI G'TCGA_C
Cuts at: 624 624
Size: 3587
SapI GCTCTTCn'nnn_
Cuts at: 1610 1820 1610
Size: 210 3377
SbfI CC_TGCA'GG
Cuts at: 634 634
Size: 3587
ScaI AGT'ACT
Cuts at: 3104 3104
Size: 3587
SfcI C'TryA_G
Cuts at: 33 555 630 1315 2308 2499 33
Size: 522 75 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 191 75
SfoI GGC'GCC
Cuts at: 1019 1267 1019
Size: 248 3339
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
SmaI CCC'GGG
Cuts at: 609 609
Size: 3587
SmlI C'TyrA_G
Cuts at: 742 1657 2149 2411 742
Size: 915 492 262 1918
Fragments arranged by size:
1918 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3587
SphI G_CATG'C
Cuts at: 640 1140 640
Size: 500 3087
StyI C'CwwG_G
Cuts at: 107 721 1075 107
Size: 614 354 2619
Fragments arranged by size:
2619 614 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 183 1170 1303 1762 1971 183
Size: 987 133 459 209 1799
Fragments arranged by size:
1799 987 459 209 133
TatI w'GTAC_w
Cuts at: 3102 3102
Size: 3587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3555
Tth111I GACn'n_nGTC
Cuts at: 1384 1384
Size: 3587
XbaI T'CTAG_A
Cuts at: 618 618
Size: 3587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1082 1082
Size: 3587
XmaI C'CCGG_G
Cuts at: 607 607
Size: 3587
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI