Restriction map for
pF1KB5474
(Circular) (Six-base) MAPSORT of: pF1KB5474.seq Check: 3226 from: 1 to: 3851
pF1KB5474 3851 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 889 889
Size: 3851
Acc65I G'GTAC_C
Cuts at: 867 867
Size: 3851
AclI AA'CG_TT
Cuts at: 845 3691 845
Size: 2846 1005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1676 2108 2855 3123 524
Size: 1152 432 747 268 1252
Fragments arranged by size:
1252 1152 747 432 268
AflIII A'CryG_T
Cuts at: 1203 2307 3284 1203
Size: 1104 977 1770
Fragments arranged by size:
1770 1104 977
AgeI A'CCGG_T
Cuts at: 2239 2239
Size: 3851
AlwNI CAG_nnn'CTG
Cuts at: 1156 2723 1156
Size: 1567 2284
ApaLI G'TGCA_C
Cuts at: 2621 2621
Size: 3851
ApoI r'AATT_y
Cuts at: 630 855 3204 3460 630
Size: 225 2349 256 1021
Fragments arranged by size:
2349 1021 256 225
AseI AT'TA_AT
Cuts at: 20 20
Size: 3851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
AvaI C'yCGr_G
Cuts at: 871 871
Size: 3851
BamHI G'GATC_C
Cuts at: 876 876
Size: 3851
BanI G'GyrC_C
Cuts at: 271 867 1281 1529 1564 271
Size: 596 414 248 35 2558
Fragments arranged by size:
2558 596 414 248 35
BanII G_rGCy'C
Cuts at: 865 1895 865
Size: 1030 2821
BbeI G_GCGC'C
Cuts at: 275 1285 1533 275
Size: 1010 248 2593
Fragments arranged by size:
2593 1010 248
BciVI GTATCCnnnnn_n'
Cuts at: 717 1749 2510 3851 717
Size: 1032 761 1341 717
Fragments arranged by size:
1341 1032 761 717
BclI T'GATC_A
Cuts at: 761 1371 761
Size: 610 3241
BglI GCCn_nnn'nGGC
Cuts at: 193 193
Size: 3851
BglII A'GATC_T
Cuts at: 1366 3403 1366
Size: 2037 1814
BlpI GC'TnA_GC
Cuts at: 152 161 963 152
Size: 9 802 3040
Fragments arranged by size:
3040 802 9
Bme1580I G_kGCm'C
Cuts at: 1476 1569 2625 1476
Size: 93 1056 2702
Fragments arranged by size:
2702 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1063 1144 1479 3623 1063
Size: 81 335 2144 1291
Fragments arranged by size:
2144 1291 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 3851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 427 536 285
Size: 142 109 3600
Fragments arranged by size:
3600 142 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1906 2398 2696 1027
Size: 879 492 298 2182
Fragments arranged by size:
2182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3555 3555
Size: 3851
BsaAI yAC'GTr
Cuts at: 1204 1204
Size: 3851
BsaBI GATnn'nnATC
Cuts at: 6 1365 3402 6
Size: 1359 2037 455
Fragments arranged by size:
2037 1359 455
BsaHI Gr'CG_yC
Cuts at: 272 1282 1530 2232 3728 272
Size: 1010 248 702 1496 395
Fragments arranged by size:
1496 1010 702 395 248
BsaWI w'CCGG_w
Cuts at: 1040 1561 2239 2513 2660 3071 1040
Size: 521 678 274 147 411 1820
Fragments arranged by size:
1820 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 830 860 830
Size: 30 3821
BseYI C'CCAG_C
Cuts at: 548 829 1276 2034 2611 3107 548
Size: 281 447 758 577 496 1292
Fragments arranged by size:
1292 758 577 496 447 281
BsiEI CG_ry'CG
Cuts at: 104 1393 1439 2647 104
Size: 1289 46 1208 1308
Fragments arranged by size:
1308 1289 1208 46
BsiHKAI G_wGCw'C
Cuts at: 865 1643 1833 2625 865
Size: 778 190 792 2091
Fragments arranged by size:
2091 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 809 818 809
Size: 9 3842
Bsp1286I G_dGCh'C
Cuts at: 865 1476 1569 1643 1833 1895 2625 865
Size: 611 93 74 190 62 730 2091
Fragments arranged by size:
2091 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3071 3071
Size: 3851
BspHI T'CATG_A
Cuts at: 3027 3027
Size: 3851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1115 1417 1798 901
Size: 214 302 381 2954
Fragments arranged by size:
2954 381 302 214
BsrBI CCG'CTC
Cuts at: 50 281 2143 2197 3681 3846 50
Size: 231 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 402 1763 402
Size: 1361 2490
BsrFI r'CCGG_y
Cuts at: 1849 2030 2239 1849
Size: 181 209 3461
Fragments arranged by size:
3461 209 181
BssSI C'ACGA_G
Cuts at: 2122 2480 2122
Size: 358 3493
BstAPI GCAn_nnn'nTGC
Cuts at: 1228 1228
Size: 3851
BstBI TT'CG_AA
Cuts at: 2212 2212
Size: 3851
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3851
BstYI r'GATC_y
Cuts at: 1 876 1343 1366 1701 1947 2959 3045 3057
Size: 875 467 23 335 246 1012 86 12
Cuts at: 3057 3403 1
Size: 346 449
Fragments arranged by size:
1012 875 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1801 1982 2145 1801
Size: 181 163 3507
Fragments arranged by size:
3507 181 163
BtsI GCAGTG_nn'
Cuts at: 1094 1094
Size: 3851
DraI TTT'AAA
Cuts at: 850 850
Size: 3851
DraIII CAC_nnn'GTG
Cuts at: 794 794
Size: 3851
DrdI GACnn_nn'nnGTC
Cuts at: 1557 2415 1557
Size: 858 2993
EaeI y'GGCC_r
Cuts at: 1436 1610 2001 2028 3373 1436
Size: 174 391 27 1345 1914
Fragments arranged by size:
1914 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1436 1436
Size: 3851
EarI CTCTTCn'nnn_
Cuts at: 224 288 731 1874 2084 224
Size: 64 443 1143 210 1991
Fragments arranged by size:
1991 1143 443 210 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2059 2369 2515 3661 2059
Size: 310 146 1146 2249
Fragments arranged by size:
2249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 863 863
Size: 3851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 427 524 536 1676 2108 2855 3123 285
Size: 142 97 12 1140 432 747 268 1013
Fragments arranged by size:
1140 1013 747 432 268 142 97 12
EcoO109I rG'GnC_Cy
Cuts at: 990 990
Size: 3851
EcoRI G'AATT_C
Cuts at: 855 855
Size: 3851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3851
FspI TGC'GCA
Cuts at: 1632 1632
Size: 3851
HaeII r_GCGC'y
Cuts at: 275 1285 1533 2555 3534 275
Size: 1010 248 1022 979 592
Fragments arranged by size:
1022 1010 979 592 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1693 1723 1725 1755 1693
Size: 30 2 30 3789
Fragments arranged by size:
3789 30 30 2
HincII GTy'rAC
Cuts at: 890 890
Size: 3851
KasI G'GCGC_C
Cuts at: 271 1281 1529 271
Size: 1010 248 2593
Fragments arranged by size:
2593 1010 248
KpnI G_GTAC'C
Cuts at: 871 871
Size: 3851
MfeI C'AATT_G
Cuts at: 710 710
Size: 3851
MluI A'CGCG_T
Cuts at: 3284 3284
Size: 3851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 395 2522 2706 248
Size: 147 2127 184 1393
Fragments arranged by size:
2127 1393 184 147
MscI TGG'CCA
Cuts at: 1612 1612
Size: 3851
MslI CAynn'nnrTG
Cuts at: 112 1840 1967 112
Size: 1728 127 1996
Fragments arranged by size:
1996 1728 127
MspA1I CmG'CkG
Cuts at: 962 1276 1636 2649 2894 962
Size: 314 360 1013 245 1919
Fragments arranged by size:
1919 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 3851
NarI GG'CG_CC
Cuts at: 272 1282 1530 272
Size: 1010 248 2593
Fragments arranged by size:
2593 1010 248
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 3851
NspI r_CATG'y
Cuts at: 904 1404 2311 904
Size: 500 907 2444
Fragments arranged by size:
2444 907 500
PciI A'CATG_T
Cuts at: 2307 2307
Size: 3851
PmeI GTTT'AAAC
Cuts at: 850 850
Size: 3851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3819
PsiI TTA'TAA
Cuts at: 746 746
Size: 3851
PstI C_TGCA'G
Cuts at: 186 386 898 1583 186
Size: 200 512 685 2454
Fragments arranged by size:
2454 685 512 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3851
PvuII CAG'CTG
Cuts at: 1276 1636 1276
Size: 360 3491
RsrII CG'GwC_CG
Cuts at: 2046 2046
Size: 3851
SacI G_AGCT'C
Cuts at: 865 865
Size: 3851
SalI G'TCGA_C
Cuts at: 888 888
Size: 3851
SapI GCTCTTCn'nnn_
Cuts at: 288 1874 2084 288
Size: 1586 210 2055
Fragments arranged by size:
2055 1586 210
SbfI CC_TGCA'GG
Cuts at: 898 898
Size: 3851
ScaI AGT'ACT
Cuts at: 3368 3368
Size: 3851
SfcI C'TryA_G
Cuts at: 33 182 382 678 894 1579 2572 2763 33
Size: 149 200 296 216 685 993 191 1121
Fragments arranged by size:
1121 993 685 296 216 200 191 149
SfoI GGC'GCC
Cuts at: 273 1283 1531 273
Size: 1010 248 2593
Fragments arranged by size:
2593 1010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3851
SmaI CCC'GGG
Cuts at: 873 873
Size: 3851
SmlI C'TyrA_G
Cuts at: 1006 1921 2413 2675 1006
Size: 915 492 262 2182
Fragments arranged by size:
2182 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3851
SphI G_CATG'C
Cuts at: 904 1404 904
Size: 500 3351
StuI AGG'CCT
Cuts at: 676 676
Size: 3851
StyI C'CwwG_G
Cuts at: 985 1339 985
Size: 354 3497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 620 1434 1567 2026 2235 620
Size: 814 133 459 209 2236
Fragments arranged by size:
2236 814 459 209 133
TatI w'GTAC_w
Cuts at: 3366 3366
Size: 3851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 3819
Tth111I GACn'n_nGTC
Cuts at: 1648 1648
Size: 3851
XbaI T'CTAG_A
Cuts at: 882 882
Size: 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1346 1346
Size: 3851
XmaI C'CCGG_G
Cuts at: 871 871
Size: 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI