Restriction map for
pF1KB5487
(Circular) (Six-base) MAPSORT of: pF1KB5487.seq Check: 4693 from: 1 to: 3857
pF1KB5487 3857 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 677 895 677
Size: 218 3639
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 817 1682 2114 2861 3129 340
Size: 477 865 432 747 268 1068
Fragments arranged by size:
1068 865 747 477 432 268
AflIII A'CryG_T
Cuts at: 125 1209 2313 3290 125
Size: 1084 1104 977 692
Fragments arranged by size:
1104 1084 977 692
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 445 795 1162 2729 445
Size: 350 367 1567 1573
Fragments arranged by size:
1573 1567 367 350
ApaLI G'TGCA_C
Cuts at: 129 2627 129
Size: 2498 1359
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 739 877 739
Size: 138 3719
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 187 226 532 873 1287 1535 1570 187
Size: 39 306 341 414 248 35 2474
Fragments arranged by size:
2474 414 341 306 248 39 35
BanII G_rGCy'C
Cuts at: 871 1901 871
Size: 1030 2827
BbeI G_GCGC'C
Cuts at: 191 230 1291 1539 191
Size: 39 1061 248 2509
Fragments arranged by size:
2509 1061 248 39
BbvCI CC'TCA_GC
Cuts at: 599 599
Size: 3857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3823
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 133 535 1482 1575 2631 133
Size: 402 947 93 1056 1359
Fragments arranged by size:
1359 1056 947 402 93
BmgBI CAC'GTC
Cuts at: 693 693
Size: 3857
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BmtI G_CTAG'C
Cuts at: 619 619
Size: 3857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 356
Size: 3857
Bpu10I CC'TnA_GC
Cuts at: 400 599 400
Size: 199 3658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 513 1210 513
Size: 697 3160
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 188 227 1288 1536 2238 3734 188
Size: 39 1061 248 702 1496 311
Fragments arranged by size:
1496 1061 702 311 248 39
BsaWI w'CCGG_w
Cuts at: 260 1046 1567 2245 2519 2666 3077 260
Size: 786 521 678 274 147 411 1040
Fragments arranged by size:
1040 786 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 816 816
Size: 3857
BseYI C'CCAG_C
Cuts at: 434 1282 2040 2617 3113 434
Size: 848 758 577 496 1178
Fragments arranged by size:
1178 848 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3857
BsiEI CG_ry'CG
Cuts at: 104 198 1399 1445 2653 104
Size: 94 1201 46 1208 1308
Fragments arranged by size:
1308 1208 1201 94 46
BsiHKAI G_wGCw'C
Cuts at: 133 871 1649 1839 2631 133
Size: 738 778 190 792 1359
Fragments arranged by size:
1359 792 778 738 190
BsmI GAATG_Cn'
Cuts at: 428 428
Size: 3857
Bsp1286I G_dGCh'C
Cuts at: 133 535 871 1482 1575 1649 1839 1901 2631
Size: 402 336 611 93 74 190 62 730
Cuts at: 2631 133
Size: 1359
Fragments arranged by size:
1359 730 611 402 336 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 907 1121 1423 1804 295
Size: 612 214 302 381 2348
Fragments arranged by size:
2348 612 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 193 1855 2036 2245 193
Size: 1662 181 209 1805
Fragments arranged by size:
1805 1662 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1234
Size: 3857
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstXI CCAn_nnnn'nTGG
Cuts at: 237 237
Size: 3857
BstYI r'GATC_y
Cuts at: 1 882 1349 1372 1707 1953 2965 3051 3063
Size: 881 467 23 335 246 1012 86 12
Cuts at: 3063 3409 1
Size: 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 164 218 164
Size: 54 3803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 120 1807 1988 2151 120
Size: 1687 181 163 1826
Fragments arranged by size:
1826 1687 181 163
BtsI GCAGTG_nn'
Cuts at: 414 1100 414
Size: 686 3171
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 195 557 1442 1616 2007 2034 3379 195
Size: 362 885 174 391 27 1345 673
Fragments arranged by size:
1345 885 673 391 362 174 27
EagI C'GGCC_G
Cuts at: 195 1442 195
Size: 1247 2610
EarI CTCTTCn'nnn_
Cuts at: 359 793 1880 2090 359
Size: 434 1087 210 2126
Fragments arranged by size:
2126 1087 434 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 356 817 1682 2114 2861 3129 340
Size: 16 461 865 432 747 268 1068
Fragments arranged by size:
1068 865 747 461 432 268 16
EcoO109I rG'GnC_Cy
Cuts at: 792 996 792
Size: 204 3653
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 191 230 686 1291 1539 2561 3540 191
Size: 39 456 605 248 1022 979 508
Fragments arranged by size:
1022 979 605 508 456 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1699 1729 1731 1761 1699
Size: 30 2 30 3795
Fragments arranged by size:
3795 30 30 2
HincII GTy'rAC
Cuts at: 594 896 594
Size: 302 3555
KasI G'GCGC_C
Cuts at: 187 226 1287 1535 187
Size: 39 1061 248 2509
Fragments arranged by size:
2509 1061 248 39
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 2528 2712 197
Size: 2331 184 1342
Fragments arranged by size:
2331 1342 184
MscI TGG'CCA
Cuts at: 559 1618 559
Size: 1059 2798
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 166 233 417 968 1282 1642 2655 2900 166
Size: 67 184 551 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 551 360 314 245 184 67
NaeI GCC'GGC
Cuts at: 195 2038 195
Size: 1843 2014
NarI GG'CG_CC
Cuts at: 188 227 1288 1536 188
Size: 39 1061 248 2509
Fragments arranged by size:
2509 1061 248 39
NgoMIV G'CCGG_C
Cuts at: 193 2036 193
Size: 1843 2014
NheI G'CTAG_C
Cuts at: 615 615
Size: 3857
NspI r_CATG'y
Cuts at: 129 627 789 910 1410 2317 129
Size: 498 162 121 500 907 1669
Fragments arranged by size:
1669 907 500 498 162 121
PciI A'CATG_T
Cuts at: 125 2313 125
Size: 2188 1669
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PmlI CAC'GTG
Cuts at: 513 513
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PpuMI rG'GwC_Cy
Cuts at: 792 792
Size: 3857
PsiI TTA'TAA
Cuts at: 668 668
Size: 3857
PstI C_TGCA'G
Cuts at: 409 614 904 1589 409
Size: 205 290 685 2677
Fragments arranged by size:
2677 685 290 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 417 1282 1642 417
Size: 865 360 2632
Fragments arranged by size:
2632 865 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SacII CC_GC'GG
Cuts at: 167 167
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 359 1880 2090 359
Size: 1521 210 2126
Fragments arranged by size:
2126 1521 210
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 405 610 678 900 1585 2578 2769 33
Size: 372 205 68 222 685 993 191 1121
Fragments arranged by size:
1121 993 685 372 222 205 191 68
SfoI GGC'GCC
Cuts at: 189 228 1289 1537 189
Size: 39 1061 248 2509
Fragments arranged by size:
2509 1061 248 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 1012 1927 2419 2681 1012
Size: 915 492 262 2188
Fragments arranged by size:
2188 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 627 910 1410 627
Size: 283 500 3074
Fragments arranged by size:
3074 500 283
StyI C'CwwG_G
Cuts at: 991 1345 991
Size: 354 3503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 795 1440 1573 2032 2241 795
Size: 645 133 459 209 2411
Fragments arranged by size:
2411 645 459 209 133
TatI w'GTAC_w
Cuts at: 733 3372 733
Size: 2639 1218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 3374 3406 242
Size: 32 3100 32 693
Fragments arranged by size:
3100 693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PmeI PmlI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI