Restriction map for
pF1KB5490
(Circular) (Six-base) MAPSORT of: pF1KB5490.seq Check: 6561 from: 1 to: 4595
pF1KB5490 4595 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1633 1633
Size: 4595
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 737 2420 2852 3599 3867 445
Size: 292 1683 432 747 268 1173
Fragments arranged by size:
1683 1173 747 432 292 268
AflIII A'CryG_T
Cuts at: 163 1361 1947 3051 4028 163
Size: 1198 586 1104 977 730
Fragments arranged by size:
1198 1104 977 730 586
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AhdI GACnn_n'nnGTC
Cuts at: 1278 1435 1278
Size: 157 4438
AlwNI CAG_nnn'CTG
Cuts at: 576 690 844 1087 1900 3467 576
Size: 114 154 243 813 1567 1704
Fragments arranged by size:
1704 1567 813 243 154 114
ApaLI G'TGCA_C
Cuts at: 1190 3365 1190
Size: 2175 2420
ApoI r'AATT_y
Cuts at: 243 564 954 981 1517 1541 1599 3948 4204
Size: 321 390 27 536 24 58 2349 256
Cuts at: 4204 243
Size: 634
Fragments arranged by size:
2349 634 536 390 321 256 58 27
24
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 1615 1615
Size: 4595
AvrII C'CTAG_G
Cuts at: 1222 1222
Size: 4595
BamHI G'GATC_C
Cuts at: 1305 1620 1305
Size: 315 4280
BanI G'GyrC_C
Cuts at: 1327 1611 2025 2273 2308 1327
Size: 284 414 248 35 3614
Fragments arranged by size:
3614 414 284 248 35
BanII G_rGCy'C
Cuts at: 1451 1609 2639 1451
Size: 158 1030 3407
Fragments arranged by size:
3407 1030 158
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbsI GAAGACnn'nnnn_
Cuts at: 743 1499 1549 743
Size: 756 50 3789
Fragments arranged by size:
3789 756 50
BciVI GTATCCnnnnn_n'
Cuts at: 535 2493 3254 4595 535
Size: 1958 761 1341 535
Fragments arranged by size:
1958 1341 761 535
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BglII A'GATC_T
Cuts at: 125 420 2110 4147 125
Size: 295 1690 2037 573
Fragments arranged by size:
2037 1690 573 295
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 1194 1332 2220 2313 3369 1194
Size: 138 888 93 1056 2420
Fragments arranged by size:
2420 1056 888 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 720 1807 1888 2223 4367 720
Size: 1087 81 335 2144 948
Fragments arranged by size:
2144 1087 948 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 4595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 647
Size: 4595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1771 2650 3142 3440 1771
Size: 879 492 298 2926
Fragments arranged by size:
2926 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4299 4299
Size: 4595
BsaAI yAC'GTr
Cuts at: 1948 1948
Size: 4595
BsaBI GATnn'nnATC
Cuts at: 6 2109 4146 6
Size: 2103 2037 455
Fragments arranged by size:
2103 2037 455
BsaHI Gr'CG_yC
Cuts at: 2026 2274 2976 4472 2026
Size: 248 702 1496 2149
Fragments arranged by size:
2149 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 833 863 833
Size: 30 4565
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1053 1056 1053
Size: 3 4592
BseYI C'CCAG_C
Cuts at: 2020 2778 3355 3851 2020
Size: 758 577 496 2764
Fragments arranged by size:
2764 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 703 161
Size: 542 4053
BsiEI CG_ry'CG
Cuts at: 104 2137 2183 3391 104
Size: 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 710 825 1194 1609 2387 2577 3369 710
Size: 115 369 415 778 190 792 1936
Fragments arranged by size:
1936 792 778 415 369 190 115
BsmI GAATG_Cn'
Cuts at: 1117 1388 1117
Size: 271 4324
Bsp1286I G_dGCh'C
Cuts at: 710 825 1194 1332 1451 1609 2220 2313 2387
Size: 115 369 138 119 158 611 93 74
Cuts at: 2387 2577 2639 3369 710
Size: 190 62 730 1936
Fragments arranged by size:
1936 730 611 369 190 158 138 119
115 93 74 62
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 3771 3771
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1002 1645 1859 2161 2542 1002
Size: 643 214 302 381 3055
Fragments arranged by size:
3055 643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 2507 173
Size: 2334 2261
BsrFI r'CCGG_y
Cuts at: 2593 2774 2983 2593
Size: 181 209 4205
Fragments arranged by size:
4205 209 181
BssSI C'ACGA_G
Cuts at: 1288 2866 3224 1288
Size: 1578 358 2659
Fragments arranged by size:
2659 1578 358
BstAPI GCAn_nnn'nTGC
Cuts at: 778 1972 778
Size: 1194 3401
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstXI CCAn_nnnn'nTGG
Cuts at: 889 889
Size: 4595
BstYI r'GATC_y
Cuts at: 1 125 420 728 1242 1305 1563 1620 2087
Size: 124 295 308 514 63 258 57 467
Cuts at: 2087 2110 2445 2691 3703 3789 3801 4147 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 514 467 449 346 335 308 295
258 246 124 86 63 57 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2545 2726 2889 2545
Size: 181 163 4251
Fragments arranged by size:
4251 181 163
BtsI GCAGTG_nn'
Cuts at: 1838 1838
Size: 4595
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DraIII CAC_nnn'GTG
Cuts at: 1355 1355
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 2301 3159 2301
Size: 858 3737
EaeI y'GGCC_r
Cuts at: 2180 2354 2745 2772 4117 2180
Size: 174 391 27 1345 2658
Fragments arranged by size:
2658 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 848 2618 2828 848
Size: 1770 210 2615
Fragments arranged by size:
2615 1770 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2803 3113 3259 4405 2803
Size: 310 146 1146 2993
Fragments arranged by size:
2993 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1607 1607
Size: 4595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 647 737 2420 2852 3599 3867 445
Size: 202 90 1683 432 747 268 1173
Fragments arranged by size:
1683 1173 747 432 268 202 90
EcoO109I rG'GnC_Cy
Cuts at: 157 618 687 1734 157
Size: 461 69 1047 3018
Fragments arranged by size:
3018 1047 461 69
EcoRI G'AATT_C
Cuts at: 243 1517 1599 243
Size: 1274 82 3239
Fragments arranged by size:
3239 1274 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 373 1166 1431 2614 373
Size: 793 265 1183 2354
Fragments arranged by size:
2354 1183 793 265
FspI TGC'GCA
Cuts at: 2376 2376
Size: 4595
HaeII r_GCGC'y
Cuts at: 2029 2277 3299 4278 2029
Size: 248 1022 979 2346
Fragments arranged by size:
2346 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 713 745 1264 1296 1421 1453 2437 2467 2469
Size: 32 519 32 125 32 984 30 2
Cuts at: 2469 2499 713
Size: 30 2809
Fragments arranged by size:
2809 984 519 125 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1634 1634
Size: 4595
HindIII A'AGCT_T
Cuts at: 437 437
Size: 4595
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MfeI C'AATT_G
Cuts at: 1490 1490
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 132 406 605 3266 3450 132
Size: 274 199 2661 184 1277
Fragments arranged by size:
2661 1277 274 199 184
MscI TGG'CCA
Cuts at: 2356 2356
Size: 4595
MslI CAynn'nnrTG
Cuts at: 1021 1205 1256 2584 2711 1021
Size: 184 51 1328 127 2905
Fragments arranged by size:
2905 1328 184 127 51
MspA1I CmG'CkG
Cuts at: 1706 2020 2380 3393 3638 1706
Size: 314 360 1013 245 2663
Fragments arranged by size:
2663 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NdeI CA'TA_TG
Cuts at: 182 1311 1524 182
Size: 1129 213 3253
Fragments arranged by size:
3253 1129 213
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NspI r_CATG'y
Cuts at: 167 1322 1365 1648 2148 3055 167
Size: 1155 43 283 500 907 1707
Fragments arranged by size:
1707 1155 907 500 283 43
PciI A'CATG_T
Cuts at: 163 1361 3051 163
Size: 1198 1690 1707
Fragments arranged by size:
1707 1690 1198
PfoI T'CCnGG_A
Cuts at: 1054 1439 1054
Size: 385 4210
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4563
PpuMI rG'GwC_Cy
Cuts at: 157 618 157
Size: 461 4134
PstI C_TGCA'G
Cuts at: 1642 2327 1642
Size: 685 3910
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 2020 2380 2020
Size: 360 4235
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1609 1609
Size: 4595
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 2618 2828 2618
Size: 210 4385
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 641 4112 641
Size: 3471 1124
SfcI C'TryA_G
Cuts at: 33 1638 2323 3316 3507 33
Size: 1605 685 993 191 1121
Fragments arranged by size:
1605 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 1750 2665 3157 3419 1750
Size: 915 492 262 2926
Fragments arranged by size:
2926 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 1648 2148 1648
Size: 500 4095
SspI AAT'ATT
Cuts at: 330 1174 330
Size: 844 3751
StyI C'CwwG_G
Cuts at: 1222 1301 1729 2083 1222
Size: 79 428 354 3734
Fragments arranged by size:
3734 428 354 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2178 2311 2770 2979 2178
Size: 133 459 209 3794
Fragments arranged by size:
3794 459 209 133
TatI w'GTAC_w
Cuts at: 639 4110 639
Size: 3471 1124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4563
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
XmnI GAAnn'nnTTC
Cuts at: 1388 1388
Size: 4595
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI