Restriction map for
pF1KB5499
(Circular) (Six-base) MAPSORT of: pF1KB5499.seq Check: 3277 from: 1 to: 7940
pF1KB5499 7940 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 410 1803 410
Size: 1393 6547
AccI GT'mk_AC
Cuts at: 1049 4978 1049
Size: 3929 4011
Acc65I G'GTAC_C
Cuts at: 4956 4956
Size: 7940
AclI AA'CG_TT
Cuts at: 7780 7780
Size: 7940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1730 1965 2677 3059 3070 4175 4325 5765
Size: 288 235 712 382 11 1105 150 1440
Cuts at: 5765 6197 6944 7212 1442
Size: 432 747 268 2170
Fragments arranged by size:
2170 1440 1105 747 712 432 382 288
268 235 150 11
AfeI AGC'GCT
Cuts at: 253 3007 4410 253
Size: 2754 1403 3783
Fragments arranged by size:
3783 2754 1403
AflII C'TTAA_G
Cuts at: 1911 1911
Size: 7940
AflIII A'CryG_T
Cuts at: 308 310 5292 6396 7373 308
Size: 2 4982 1104 977 875
Fragments arranged by size:
4982 1104 977 875 2
AgeI A'CCGG_T
Cuts at: 2693 6328 2693
Size: 3635 4305
AleI CACnn'nnGTG
Cuts at: 547 3087 547
Size: 2540 5400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2763 4832 2763
Size: 2069 5871
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 649 681 649
Size: 32 7908
AlwNI CAG_nnn'CTG
Cuts at: 3843 4341 4447 5245 6812 3843
Size: 498 106 798 1567 4971
Fragments arranged by size:
4971 1567 798 498 106
ApaI G_GGCC'C
Cuts at: 620 620
Size: 7940
ApaLI G'TGCA_C
Cuts at: 225 2481 6710 225
Size: 2256 4229 1455
Fragments arranged by size:
4229 2256 1455
ApoI r'AATT_y
Cuts at: 730 1003 2035 3399 4944 7293 7549 730
Size: 273 1032 1364 1545 2349 256 1121
Fragments arranged by size:
2349 1545 1364 1121 1032 273 256
AseI AT'TA_AT
Cuts at: 20 4658 20
Size: 4638 3302
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7940
AvaI C'yCGr_G
Cuts at: 146 262 325 1574 1720 3860 4127 4960 146
Size: 116 63 1249 146 2140 267 833 3126
Fragments arranged by size:
3126 2140 1249 833 267 146 116 63
BamHI G'GATC_C
Cuts at: 4965 4965
Size: 7940
BanI G'GyrC_C
Cuts at: 1234 1250 2401 2500 4726 4956 5370 5618 5653
Size: 16 1151 99 2226 230 414 248 35
Cuts at: 5653 1234
Size: 3521
Fragments arranged by size:
3521 2226 1151 414 248 230 99 35
16
BanII G_rGCy'C
Cuts at: 620 1321 2525 2932 3934 4954 5984 620
Size: 701 1204 407 1002 1020 1030 2576
Fragments arranged by size:
2576 1204 1030 1020 1002 701 407
BbeI G_GCGC'C
Cuts at: 1238 5374 5622 1238
Size: 4136 248 3556
Fragments arranged by size:
4136 3556 248
BbsI GAAGACnn'nnnn_
Cuts at: 617 1057 1892 1971 4894 617
Size: 440 835 79 2923 3663
Fragments arranged by size:
3663 2923 835 440 79
BbvCI CC'TCA_GC
Cuts at: 4648 4648
Size: 7940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4594 4628 4594
Size: 34 7906
BciVI GTATCCnnnnn_n'
Cuts at: 4173 4683 5838 6599 7940 4173
Size: 510 1155 761 1341 4173
Fragments arranged by size:
4173 1341 1155 761 510
BclI T'GATC_A
Cuts at: 3237 5460 3237
Size: 2223 5717
BglI GCCn_nnn'nGGC
Cuts at: 133 150 178 193 1199 2981 3883 3919 4336
Size: 17 28 15 1006 1782 902 36 417
Cuts at: 4336 4369 4390 133
Size: 33 21 3683
Fragments arranged by size:
3683 1782 1006 902 417 36 33 28
21 17 15
BglII A'GATC_T
Cuts at: 3905 4473 5455 7492 3905
Size: 568 982 2037 4353
Fragments arranged by size:
4353 2037 982 568
BlpI GC'TnA_GC
Cuts at: 3923 3928 5052 3923
Size: 5 1124 6811
Fragments arranged by size:
6811 1124 5
Bme1580I G_kGCm'C
Cuts at: 207 229 620 2485 3516 5565 5658 6714 207
Size: 22 391 1865 1031 2049 93 1056 1433
Fragments arranged by size:
2049 1865 1433 1056 1031 391 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 764 3299 5152 5233 5568 7712 764
Size: 2535 1853 81 335 2144 992
Fragments arranged by size:
2535 2144 1853 992 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1746 2036 3268 6307 1746
Size: 290 1232 3039 3379
Fragments arranged by size:
3379 3039 1232 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2456 3238 3973 4102 4810 4894 2168
Size: 288 782 735 129 708 84 5214
Fragments arranged by size:
5214 782 735 708 288 129 84
Bpu10I CC'TnA_GC
Cuts at: 1937 4648 1937
Size: 2711 5229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 901 1918 3583 4580 5116 5995 6487 6785
Size: 360 1017 1665 997 536 879 492 298
Cuts at: 6785 541
Size: 1696
Fragments arranged by size:
1696 1665 1017 997 879 536 492 360
298
BsaI GGTCTCn'nnnn_
Cuts at: 1954 2293 2540 7644 1954
Size: 339 247 5104 2250
Fragments arranged by size:
5104 2250 339 247
BsaAI yAC'GTr
Cuts at: 311 2077 2838 5293 311
Size: 1766 761 2455 2958
Fragments arranged by size:
2958 2455 1766 761
BsaBI GATnn'nnATC
Cuts at: 6 3544 5454 7491 6
Size: 3538 1910 2037 455
Fragments arranged by size:
3538 2037 1910 455
BsaHI Gr'CG_yC
Cuts at: 386 1235 5371 5619 6321 7817 386
Size: 849 4136 248 702 1496 509
Fragments arranged by size:
4136 1496 849 702 509 248
BsaWI w'CCGG_w
Cuts at: 2693 3467 5129 5650 6328 6602 6749 7160 2693
Size: 774 1662 521 678 274 147 411 3473
Fragments arranged by size:
3473 1662 774 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 649 679 878 908 2464 2494 484
Size: 30 135 30 199 30 1556 30 5930
Fragments arranged by size:
5930 1556 199 135 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1107 1644 2397 3886 4276 4636 4861 1107
Size: 537 753 1489 390 360 225 4186
Fragments arranged by size:
4186 1489 753 537 390 360 225
BseYI C'CCAG_C
Cuts at: 496 793 4331 4677 5365 6123 6700 7196 496
Size: 297 3538 346 688 758 577 496 1240
Fragments arranged by size:
3538 1240 758 688 577 496 346 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 502 508 2160 2388 2616 361
Size: 141 6 1652 228 228 5685
Fragments arranged by size:
5685 1652 228 228 141 6
BsiEI CG_ry'CG
Cuts at: 104 188 247 1087 4256 5482 5528 6736 104
Size: 84 59 840 3169 1226 46 1208 1308
Fragments arranged by size:
3169 1308 1226 1208 840 84 59 46
BsiHKAI G_wGCw'C
Cuts at: 229 2136 2485 2525 4954 5732 5922 6714 229
Size: 1907 349 40 2429 778 190 792 1455
Fragments arranged by size:
2429 1907 1455 792 778 349 190 40
BsmI GAATG_Cn'
Cuts at: 1126 1162 1605 2656 3118 4428 1126
Size: 36 443 1051 462 1310 4638
Fragments arranged by size:
4638 1310 1051 462 443 36
BsmBI CGTCTCn'nnnn_
Cuts at: 559 559
Size: 7940
Bsp1286I G_dGCh'C
Cuts at: 207 229 620 1321 2136 2485 2525 2932 3516
Size: 22 391 701 815 349 40 407 584
Cuts at: 3516 3934 4954 5565 5658 5732 5922 5984 6714
Size: 418 1020 611 93 74 190 62 730
Cuts at: 6714 207
Size: 1433
Fragments arranged by size:
1433 1020 815 730 701 611 584 418
407 391 349 190 93 74 62 40
22
BspEI T'CCGG_A
Cuts at: 3467 7160 3467
Size: 3693 4247
BspHI T'CATG_A
Cuts at: 4853 7116 4853
Size: 2263 5677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 1803 3755 4990 5204 5506 5887 410
Size: 1393 1952 1235 214 302 381 2463
Fragments arranged by size:
2463 1952 1393 1235 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3534 6232 6286 7770 7935 50
Size: 3484 2698 54 1484 165 55
Fragments arranged by size:
3484 2698 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1146 1784 2292 5852 1146
Size: 638 508 3560 3234
Fragments arranged by size:
3560 3234 638 508
BsrFI r'CCGG_y
Cuts at: 132 1082 2693 2959 3212 3886 5938 6119 6328
Size: 950 1611 266 253 674 2052 181 209
Cuts at: 6328 132
Size: 1744
Fragments arranged by size:
2052 1744 1611 950 674 266 253 209
181
BsrGI T'GTAC_A
Cuts at: 1618 1618
Size: 7940
BssSI C'ACGA_G
Cuts at: 6211 6569 6211
Size: 358 7582
BstAPI GCAn_nnn'nTGC
Cuts at: 1439 1589 2756 5317 1439
Size: 150 1167 2561 4062
Fragments arranged by size:
4062 2561 1167 150
BstBI TT'CG_AA
Cuts at: 6301 6301
Size: 7940
BstEII G'GTnAC_C
Cuts at: 351 360 351
Size: 9 7931
BstXI CCAn_nnnn'nTGG
Cuts at: 2854 2854
Size: 7940
BstYI r'GATC_y
Cuts at: 1 3073 3840 3905 4473 4965 5432 5455 5790
Size: 3072 767 65 568 492 467 23 335
Cuts at: 5790 6036 7048 7134 7146 7492 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3072 1012 767 568 492 467 449 346
335 246 86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 3068 3068
Size: 7940
BtgI C'CryG_G
Cuts at: 191 215 678 1087 1194 191
Size: 24 463 409 107 6937
Fragments arranged by size:
6937 463 409 107 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5890 6071 6234 5890
Size: 181 163 7596
Fragments arranged by size:
7596 181 163
BtsI GCAGTG_nn'
Cuts at: 2376 2604 2672 2824 2950 4353 5183 2376
Size: 228 68 152 126 1403 830 5133
Fragments arranged by size:
5133 1403 830 228 152 126 68
ClaI AT'CG_AT
Cuts at: 2609 2609
Size: 7940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 294 329 2058 2093 2147 2182 2456 2491 294
Size: 35 1729 35 54 35 274 35 5743
Fragments arranged by size:
5743 1729 274 54 35 35 35 35
DraI TTT'AAA
Cuts at: 4939 4939
Size: 7940
DraIII CAC_nnn'GTG
Cuts at: 458 1334 1336 458
Size: 876 2 7062
Fragments arranged by size:
7062 876 2
DrdI GACnn_nn'nnGTC
Cuts at: 2261 5646 6504 2261
Size: 3385 858 3697
Fragments arranged by size:
3697 3385 858
EaeI y'GGCC_r
Cuts at: 170 185 244 299 479 1084 1191 2844 2961
Size: 15 59 55 180 605 107 1653 117
Cuts at: 2961 4253 5525 5699 6090 6117 7462 170
Size: 1292 1272 174 391 27 1345 648
Fragments arranged by size:
1653 1345 1292 1272 648 605 391 180
174 117 107 59 55 27 15
EagI C'GGCC_G
Cuts at: 185 244 1084 4253 5525 185
Size: 59 840 3169 1272 2600
Fragments arranged by size:
3169 2600 1272 840 59
EarI CTCTTCn'nnn_
Cuts at: 1299 2229 3476 3644 3791 5963 6173 1299
Size: 930 1247 168 147 2172 210 3066
Fragments arranged by size:
3066 2172 1247 930 210 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 311 1258 4372 4408 6148 6458 6604 7750 311
Size: 947 3114 36 1740 310 146 1146 501
Fragments arranged by size:
3114 1740 1146 947 501 310 146 36
EcoICRI GAG'CTC
Cuts at: 2523 4952 2523
Size: 2429 5511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1730 1965 2168 2456 2677 3059 3070 3238
Size: 288 235 203 288 221 382 11 168
Cuts at: 3238 3973 4102 4175 4325 4810 4894 5765 6197
Size: 735 129 73 150 485 84 871 432
Cuts at: 6197 6944 7212 1442
Size: 747 268 2170
Fragments arranged by size:
2170 871 747 735 485 432 382 288
288 268 235 221 203 168 150 129
84 73 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1734 2315 3367 1734
Size: 581 1052 6307
Fragments arranged by size:
6307 1052 581
EcoO109I rG'GnC_Cy
Cuts at: 617 3621 4032 4146 4338 5079 617
Size: 3004 411 114 192 741 3478
Fragments arranged by size:
3478 3004 741 411 192 114
EcoRI G'AATT_C
Cuts at: 730 4944 730
Size: 4214 3726
EcoRV GAT'ATC
Cuts at: 1746 4842 1746
Size: 3096 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1995 5959 1995
Size: 3964 3976
FspI TGC'GCA
Cuts at: 5721 5721
Size: 7940
HaeII r_GCGC'y
Cuts at: 255 800 1238 3009 4412 5374 5622 6644 7623
Size: 545 438 1771 1403 962 248 1022 979
Cuts at: 7623 255
Size: 572
Fragments arranged by size:
1771 1403 1022 979 962 572 545 438
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1812 1844 2292 2324 2507 2539 5782 5812 5814
Size: 32 448 32 183 32 3243 30 2
Cuts at: 5814 5844 1812
Size: 30 3908
Fragments arranged by size:
3908 3243 448 183 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 273 3018 3393 3731 4979 273
Size: 2745 375 338 1248 3234
Fragments arranged by size:
3234 2745 1248 375 338
HindIII A'AGCT_T
Cuts at: 530 3661 530
Size: 3131 4809
KasI G'GCGC_C
Cuts at: 1234 5370 5618 1234
Size: 4136 248 3556
Fragments arranged by size:
4136 3556 248
KpnI G_GTAC'C
Cuts at: 4960 4960
Size: 7940
MfeI C'AATT_G
Cuts at: 1524 2760 1524
Size: 1236 6704
MluI A'CGCG_T
Cuts at: 7373 7373
Size: 7940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2604 6611 6795 2604
Size: 4007 184 3749
Fragments arranged by size:
4007 3749 184
MscI TGG'CCA
Cuts at: 301 1193 2846 5701 301
Size: 892 1653 2855 2540
Fragments arranged by size:
2855 2540 1653 892
MslI CAynn'nnrTG
Cuts at: 547 3087 3976 4567 5929 6056 547
Size: 2540 889 591 1362 127 2431
Fragments arranged by size:
2540 2431 1362 889 591 127
MspA1I CmG'CkG
Cuts at: 193 680 1358 2798 3927 4677 4686 5051 5365
Size: 487 678 1440 1129 750 9 365 314
Cuts at: 5365 5725 6738 6983 193
Size: 360 1013 245 1150
Fragments arranged by size:
1440 1150 1129 1013 750 678 487 365
360 314 245 9
NaeI GCC'GGC
Cuts at: 134 6121 134
Size: 5987 1953
NarI GG'CG_CC
Cuts at: 1235 5371 5619 1235
Size: 4136 248 3556
Fragments arranged by size:
4136 3556 248
NcoI C'CATG_G
Cuts at: 215 1194 215
Size: 979 6961
NdeI CA'TA_TG
Cuts at: 1041 1041
Size: 7940
NgoMIV G'CCGG_C
Cuts at: 132 6119 132
Size: 5987 1953
NotI GC'GGCC_GC
Cuts at: 185 244 185
Size: 59 7881
NspI r_CATG'y
Cuts at: 261 4993 5493 6400 261
Size: 4732 500 907 1801
Fragments arranged by size:
4732 1801 907 500
PasI CC'CwG_GG
Cuts at: 2553 4341 2553
Size: 1788 6152
PciI A'CATG_T
Cuts at: 6396 6396
Size: 7940
PflMI CCAn_nnn'nTGG
Cuts at: 458 2925 458
Size: 2467 5473
PfoI T'CCnGG_A
Cuts at: 2433 3254 2433
Size: 821 7119
PmeI GTTT'AAAC
Cuts at: 4939 4939
Size: 7940
PmlI CAC'GTG
Cuts at: 311 2838 311
Size: 2527 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 649 681 7079 7111 649
Size: 32 6398 32 1478
Fragments arranged by size:
6398 1478 32 32
PpuMI rG'GwC_Cy
Cuts at: 3621 4032 3621
Size: 411 7529
PshAI GACnn'nnGTC
Cuts at: 358 358
Size: 7940
PspOMI G'GGCC_C
Cuts at: 616 616
Size: 7940
PstI C_TGCA'G
Cuts at: 407 830 2335 2400 2888 4514 4987 5672 407
Size: 423 1505 65 488 1626 473 685 2675
Fragments arranged by size:
2675 1626 1505 685 488 473 423 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7940
PvuII CAG'CTG
Cuts at: 1358 2798 3927 4677 4686 5365 5725 1358
Size: 1440 1129 750 9 679 360 3573
Fragments arranged by size:
3573 1440 1129 750 679 360 9
RsrII CG'GwC_CG
Cuts at: 6135 6135
Size: 7940
SacI G_AGCT'C
Cuts at: 2525 4954 2525
Size: 2429 5511
SacII CC_GC'GG
Cuts at: 194 681 194
Size: 487 7453
SalI G'TCGA_C
Cuts at: 4977 4977
Size: 7940
SanDI GG'GwC_CC
Cuts at: 3621 3621
Size: 7940
SapI GCTCTTCn'nnn_
Cuts at: 1299 3791 5963 6173 1299
Size: 2492 2172 210 3066
Fragments arranged by size:
3066 2492 2172 210
SbfI CC_TGCA'GG
Cuts at: 4987 4987
Size: 7940
ScaI AGT'ACT
Cuts at: 637 7457 637
Size: 6820 1120
SexAI A'CCwGG_T
Cuts at: 454 454
Size: 7940
SfcI C'TryA_G
Cuts at: 33 403 639 826 950 1305 1635 2331 2396
Size: 370 236 187 124 355 330 696 65
Cuts at: 2396 2884 3415 3614 4510 4983 5668 6661 6852
Size: 488 531 199 896 473 685 993 191
Cuts at: 6852 33
Size: 1121
Fragments arranged by size:
1121 993 896 696 685 531 488 473
370 355 330 236 199 191 187 124
65
SfiI GGCCn_nnn'nGGCC
Cuts at: 133 1199 3883 133
Size: 1066 2684 4190
Fragments arranged by size:
4190 2684 1066
SfoI GGC'GCC
Cuts at: 1236 5372 5620 1236
Size: 4136 248 3556
Fragments arranged by size:
4136 3556 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7940
SmaI CCC'GGG
Cuts at: 148 4962 148
Size: 4814 3126
SmlI C'TyrA_G
Cuts at: 556 916 1720 1897 1911 3562 4595 5095 6010
Size: 360 804 177 14 1651 1033 500 915
Cuts at: 6010 6502 6764 556
Size: 492 262 1732
Fragments arranged by size:
1732 1651 1033 915 804 500 492 360
262 177 14
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7940
SphI G_CATG'C
Cuts at: 261 4993 5493 261
Size: 4732 500 2708
Fragments arranged by size:
4732 2708 500
StuI AGG'CCT
Cuts at: 715 2016 715
Size: 1301 6639
StyI C'CwwG_G
Cuts at: 215 668 1194 1272 2008 2924 3282 4142 4436
Size: 453 526 78 736 916 358 860 294
Cuts at: 4436 5074 5428 215
Size: 638 354 2727
Fragments arranged by size:
2727 916 860 736 638 526 453 358
354 294 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 1670 2154 3164 5523 5656 6115 6324 442
Size: 1228 484 1010 2359 133 459 209 2058
Fragments arranged by size:
2359 2058 1228 1010 484 459 209 133
TatI w'GTAC_w
Cuts at: 635 721 1618 3864 7455 635
Size: 86 897 2246 3591 1120
Fragments arranged by size:
3591 2246 1120 897 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 2073 2105 2464 2475 2496 2507 7457
Size: 32 1163 32 359 11 21 11 4950
Cuts at: 7457 7489 878
Size: 32 1329
Fragments arranged by size:
4950 1329 1163 359 32 32 32 21
11 11
Tth111I GACn'n_nGTC
Cuts at: 1510 5737 1510
Size: 4227 3713
XbaI T'CTAG_A
Cuts at: 4971 4971
Size: 7940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 640 3028 5435 640
Size: 2388 2407 3145
Fragments arranged by size:
3145 2407 2388
XhoI C'TCGA_G
Cuts at: 1720 1720
Size: 7940
XmaI C'CCGG_G
Cuts at: 146 4960 146
Size: 4814 3126
XmnI GAAnn'nnTTC
Cuts at: 1264 1264
Size: 7940
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AhdI AscI AvrII BaeI BfrBI BmgBI BmtI
BsiWI BssHII BstZ17I FseI FspAI HpaI NheI NruI
NsiI PacI PsiI PspXI PsrI SgrAI SnaBI SrfI
SspI SwaI ZraI