Restriction map for
pF1KB5507
(Circular) (Six-base) MAPSORT of: pF1KB5507.seq Check: 6945 from: 1 to: 4901
pF1KB5507 4901 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4901
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 4741 4741
Size: 4901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 984 2726 3158 3905 4173 379
Size: 605 1742 432 747 268 1107
Fragments arranged by size:
1742 1107 747 605 432 268
AflIII A'CryG_T
Cuts at: 1562 2253 3357 4334 1562
Size: 691 1104 977 2129
Fragments arranged by size:
2129 1104 977 691
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1282 1282
Size: 4901
AlwNI CAG_nnn'CTG
Cuts at: 508 1122 1650 2206 3773 508
Size: 614 528 556 1567 1636
Fragments arranged by size:
1636 1567 614 556 528
ApaLI G'TGCA_C
Cuts at: 566 3671 566
Size: 3105 1796
ApoI r'AATT_y
Cuts at: 414 1079 1508 1905 4254 4510 414
Size: 665 429 397 2349 256 805
Fragments arranged by size:
2349 805 665 429 397 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4901
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 115 656 988 1921 115
Size: 541 332 933 3095
Fragments arranged by size:
3095 933 541 332
BamHI G'GATC_C
Cuts at: 954 1926 954
Size: 972 3929
BanI G'GyrC_C
Cuts at: 1917 2331 2579 2614 1917
Size: 414 248 35 4204
Fragments arranged by size:
4204 414 248 35
BanII G_rGCy'C
Cuts at: 116 176 1915 2945 116
Size: 60 1739 1030 2072
Fragments arranged by size:
2072 1739 1030 60
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbsI GAAGACnn'nnnn_
Cuts at: 1430 1430
Size: 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1245 1279 1245
Size: 34 4867
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 939 2421 939
Size: 1482 3419
BglII A'GATC_T
Cuts at: 1014 2416 4453 1014
Size: 1402 2037 1462
Fragments arranged by size:
2037 1462 1402
BlpI GC'TnA_GC
Cuts at: 175 2013 175
Size: 1838 3063
Bme1580I G_kGCm'C
Cuts at: 570 2526 2619 3675 570
Size: 1956 93 1056 1796
Fragments arranged by size:
1956 1796 1056 93
BmgBI CAC'GTC
Cuts at: 404 1563 404
Size: 1159 3742
BmrI ACTGGGnnnn_n'
Cuts at: 297 1396 1530 2113 2194 2529 4673 297
Size: 1099 134 583 81 335 2144 525
Fragments arranged by size:
2144 1099 583 525 335 134 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 4901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1144 1234 1514 961
Size: 183 90 280 4348
Fragments arranged by size:
4348 280 183 90
Bpu10I CC'TnA_GC
Cuts at: 343 1637 343
Size: 1294 3607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2956 3448 3746 2077
Size: 879 492 298 3232
Fragments arranged by size:
3232 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4605 4605
Size: 4901
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 2415 4452 6
Size: 2409 2037 455
Fragments arranged by size:
2409 2037 455
BsaHI Gr'CG_yC
Cuts at: 2332 2580 3282 4778 2332
Size: 248 702 1496 2455
Fragments arranged by size:
2455 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2090 2611 3289 3563 3710 4121 2090
Size: 521 678 274 147 411 2870
Fragments arranged by size:
2870 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1281 1311 1281
Size: 30 4871
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1481 1728 1481
Size: 247 4654
BseYI C'CCAG_C
Cuts at: 501 2326 3084 3661 4157 501
Size: 1825 758 577 496 1245
Fragments arranged by size:
1825 1245 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 1645
Size: 4901
BsiEI CG_ry'CG
Cuts at: 104 145 206 2443 2489 3697 104
Size: 41 61 2237 46 1208 1308
Fragments arranged by size:
2237 1308 1208 61 46 41
BsiHKAI G_wGCw'C
Cuts at: 570 1106 1915 2693 2883 3675 570
Size: 536 809 778 190 792 1796
Fragments arranged by size:
1796 809 792 778 536 190
BsmI GAATG_Cn'
Cuts at: 894 894
Size: 4901
Bsp1286I G_dGCh'C
Cuts at: 116 176 570 1106 1915 2526 2619 2693 2883
Size: 60 394 536 809 611 93 74 190
Cuts at: 2883 2945 3675 116
Size: 62 730 1342
Fragments arranged by size:
1342 809 730 611 536 394 190 93
74 62 60
BspEI T'CCGG_A
Cuts at: 4121 4121
Size: 4901
BspHI T'CATG_A
Cuts at: 4077 4077
Size: 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 518 1436 1951 2165 2467 2848 518
Size: 918 515 214 302 381 2571
Fragments arranged by size:
2571 918 515 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1600 3193 3247 4731 4896 50
Size: 1550 1593 54 1484 165 55
Fragments arranged by size:
1593 1550 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 2813 1604
Size: 1209 3692
BsrFI r'CCGG_y
Cuts at: 1388 2899 3080 3289 1388
Size: 1511 181 209 3000
Fragments arranged by size:
3000 1511 209 181
BssSI C'ACGA_G
Cuts at: 395 865 3172 3530 395
Size: 470 2307 358 1766
Fragments arranged by size:
2307 1766 470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1650 2278 1650
Size: 628 4273
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstEII G'GTnAC_C
Cuts at: 487 875 487
Size: 388 4513
BstYI r'GATC_y
Cuts at: 1 496 681 954 1014 1926 2393 2416 2751
Size: 495 185 273 60 912 467 23 335
Cuts at: 2751 2997 4009 4095 4107 4453 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 912 495 467 449 346 335 273
246 185 86 60 23 12
BtgI C'CryG_G
Cuts at: 107 145 1275 107
Size: 38 1130 3733
Fragments arranged by size:
3733 1130 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 996 2851 3032 3195 996
Size: 1855 181 163 2702
Fragments arranged by size:
2702 1855 181 163
BtsI GCAGTG_nn'
Cuts at: 570 1585 2144 570
Size: 1015 559 3327
Fragments arranged by size:
3327 1015 559
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 455 2607 3465 455
Size: 2152 858 1891
Fragments arranged by size:
2152 1891 858
EaeI y'GGCC_r
Cuts at: 203 1601 2486 2660 3051 3078 4423 203
Size: 1398 885 174 391 27 1345 681
Fragments arranged by size:
1398 1345 885 681 391 174 27
EagI C'GGCC_G
Cuts at: 203 2486 203
Size: 2283 2618
EarI CTCTTCn'nnn_
Cuts at: 737 938 1661 1839 2924 3134 737
Size: 201 723 178 1085 210 2504
Fragments arranged by size:
2504 1085 723 210 201 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3109 3419 3565 4711 3109
Size: 310 146 1146 3299
Fragments arranged by size:
3299 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1913 1913
Size: 4901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 961 984 1144 1234 1514 2726 3158 3905
Size: 582 23 160 90 280 1212 432 747
Cuts at: 3905 4173 379
Size: 268 1107
Fragments arranged by size:
1212 1107 747 582 432 280 268 160
90 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1692 1692
Size: 4901
EcoO109I rG'GnC_Cy
Cuts at: 196 1239 1647 2040 196
Size: 1043 408 393 3057
Fragments arranged by size:
3057 1043 408 393
EcoRI G'AATT_C
Cuts at: 1508 1905 1508
Size: 397 4504
EcoRV GAT'ATC
Cuts at: 1035 1164 1035
Size: 129 4772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 952 1099 2920 952
Size: 147 1821 2933
Fragments arranged by size:
2933 1821 147
FspI TGC'GCA
Cuts at: 2682 2682
Size: 4901
HaeII r_GCGC'y
Cuts at: 2335 2583 3605 4584 2335
Size: 248 1022 979 2652
Fragments arranged by size:
2652 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 760 792 2743 2773 2775 2805 481
Size: 32 247 32 1951 30 2 30 2577
Fragments arranged by size:
2577 1951 247 32 32 30 30 2
HincII GTy'rAC
Cuts at: 533 806 1940 533
Size: 273 1134 3494
Fragments arranged by size:
3494 1134 273
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 3572 3756 200
Size: 3372 184 1345
Fragments arranged by size:
3372 1345 184
MscI TGG'CCA
Cuts at: 2662 2662
Size: 4901
MslI CAynn'nnrTG
Cuts at: 595 865 1109 1789 2890 3017 595
Size: 270 244 680 1101 127 2479
Fragments arranged by size:
2479 1101 680 270 244 127
MspA1I CmG'CkG
Cuts at: 147 918 2012 2326 2686 3699 3944 147
Size: 771 1094 314 360 1013 245 1104
Fragments arranged by size:
1104 1094 1013 771 360 314 245
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NcoI C'CATG_G
Cuts at: 107 1275 107
Size: 1168 3733
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NspI r_CATG'y
Cuts at: 1788 1954 2454 3361 1788
Size: 166 500 907 3328
Fragments arranged by size:
3328 907 500 166
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1672 1704 4040 4072 1672
Size: 32 2336 32 2501
Fragments arranged by size:
2501 2336 32 32
PpuMI rG'GwC_Cy
Cuts at: 1239 1239
Size: 4901
PstI C_TGCA'G
Cuts at: 1433 1646 1948 2633 1433
Size: 213 302 685 3701
Fragments arranged by size:
3701 685 302 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 918 2326 2686 918
Size: 1408 360 3133
Fragments arranged by size:
3133 1408 360
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 1915 1915
Size: 4901
SacII CC_GC'GG
Cuts at: 148 148
Size: 4901
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SanDI GG'GwC_CC
Cuts at: 1239 1239
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 938 1839 2924 3134 938
Size: 901 1085 210 2705
Fragments arranged by size:
2705 1085 901 210
SbfI CC_TGCA'GG
Cuts at: 1433 1948 1433
Size: 515 4386
ScaI AGT'ACT
Cuts at: 4418 4418
Size: 4901
SexAI A'CCwGG_T
Cuts at: 1379 1379
Size: 4901
SfcI C'TryA_G
Cuts at: 33 894 906 1429 1642 1944 2629 3622 3813
Size: 861 12 523 213 302 685 993 191
Cuts at: 3813 33
Size: 1121
Fragments arranged by size:
1121 993 861 685 523 302 213 191
12
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 988 2056 2971 3463 3725 988
Size: 1068 915 492 262 2164
Fragments arranged by size:
2164 1068 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 1788 1954 2454 1788
Size: 166 500 4235
Fragments arranged by size:
4235 500 166
StuI AGG'CCT
Cuts at: 703 703
Size: 4901
StyI C'CwwG_G
Cuts at: 107 407 1275 2035 2389 107
Size: 300 868 760 354 2619
Fragments arranged by size:
2619 868 760 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2484 2617 3076 3285 2484
Size: 133 459 209 4100
Fragments arranged by size:
4100 459 209 133
TatI w'GTAC_w
Cuts at: 4416 4416
Size: 4901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1281 1313 4418 4450 1281
Size: 32 3105 32 1732
Fragments arranged by size:
3105 1732 32 32
Tth111I GACn'n_nGTC
Cuts at: 2698 2698
Size: 4901
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2396 2396
Size: 4901
XhoI C'TCGA_G
Cuts at: 988 988
Size: 4901
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI