Restriction map for
pF1KB5508
(Circular) (Six-base) MAPSORT of: pF1KB5508.seq Check: 8100 from: 1 to: 4742
pF1KB5508 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 803 863 1780 803
Size: 60 917 3765
Fragments arranged by size:
3765 917 60
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 1069 1249 2567 2999 3352 4014 142
Size: 927 180 1318 432 353 662 870
Fragments arranged by size:
1318 927 870 662 432 353 180
AflIII A'CryG_T
Cuts at: 2094 3894 4175 2094
Size: 1800 281 2661
Fragments arranged by size:
2661 1800 281
AleI CACnn'nnGTG
Cuts at: 1324 1324
Size: 4742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 339 543 792 960 1191 2047 3485 339
Size: 204 249 168 231 856 1438 1596
Fragments arranged by size:
1596 1438 856 249 231 204 168
ApaI G_GGCC'C
Cuts at: 1011 1369 1011
Size: 358 4384
ApaLI G'TGCA_C
Cuts at: 3580 3580
Size: 4742
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 151 570 847 1762 151
Size: 419 277 915 3131
Fragments arranged by size:
3131 915 419 277
BamHI G'GATC_C
Cuts at: 1767 1767
Size: 4742
BanI G'GyrC_C
Cuts at: 354 427 586 911 1354 1488 1698 1758 2172
Size: 73 159 325 443 134 210 60 414
Cuts at: 2172 2420 2455 354
Size: 248 35 2641
Fragments arranged by size:
2641 443 414 325 248 210 159 134
73 60 35
BanII G_rGCy'C
Cuts at: 383 1011 1369 1420 1729 1756 2786 383
Size: 628 358 51 309 27 1030 2339
Fragments arranged by size:
2339 1030 628 358 309 51 27
BbeI G_GCGC'C
Cuts at: 915 1492 2176 2424 915
Size: 577 684 248 3233
Fragments arranged by size:
3233 684 577 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 189 223 429 463 189
Size: 34 206 34 4468
Fragments arranged by size:
4468 206 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 586 2640 3696 4742 586
Size: 2054 1056 1046 586
Fragments arranged by size:
2054 1056 1046 586
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 1724 1724
Size: 4742
BglII A'GATC_T
Cuts at: 692 1472 2257 4294 692
Size: 780 785 2037 1140
Fragments arranged by size:
2037 1140 785 780
BlpI GC'TnA_GC
Cuts at: 1507 1582 1854 1507
Size: 75 272 4395
Fragments arranged by size:
4395 272 75
Bme1580I G_kGCm'C
Cuts at: 138 359 430 437 525 844 902 1011 1357
Size: 221 71 7 88 319 58 109 346
Cuts at: 1357 1369 2367 2460 3584 138
Size: 12 998 93 1124 1296
Fragments arranged by size:
1296 1124 998 346 319 221 109 93
88 71 58 12 7
BmrI ACTGGGnnnn_n'
Cuts at: 1954 2035 2370 4514 1954
Size: 81 335 2144 2182
Fragments arranged by size:
2182 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1080 1434 3109 1080
Size: 354 1675 2713
Fragments arranged by size:
2713 1675 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 277 364 565 709 946 1264 1333 235
Size: 42 87 201 144 237 318 69 3644
Fragments arranged by size:
3644 318 237 201 144 87 69 42
Bpu10I CC'TnA_GC
Cuts at: 377 377
Size: 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1918 2797 3511 3809 166
Size: 1752 879 714 298 1099
Fragments arranged by size:
1752 1099 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4446 4446
Size: 4742
BsaAI yAC'GTr
Cuts at: 2095 2095
Size: 4742
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 912 1489 1716 2173 2421 3123 4619 912
Size: 577 227 457 248 702 1496 1035
Fragments arranged by size:
1496 1035 702 577 457 248 227
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3541 3688 3962 1931
Size: 521 678 411 147 274 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 1711 1741 856
Size: 30 825 30 3857
Fragments arranged by size:
3857 825 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 883 943 1321 1426 232
Size: 651 60 378 105 3548
Fragments arranged by size:
3548 651 378 105 60
BseYI C'CCAG_C
Cuts at: 524 825 884 1563 2167 2925 3590 3998 524
Size: 301 59 679 604 758 665 408 1268
Fragments arranged by size:
1268 758 679 665 604 408 301 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 1354
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 626 2284 2330 3560 104
Size: 522 1658 46 1230 1286
Fragments arranged by size:
1658 1286 1230 522 46
BsiHKAI G_wGCw'C
Cuts at: 224 761 1321 1756 2534 2724 3584 224
Size: 537 560 435 778 190 860 1382
Fragments arranged by size:
1382 860 778 560 537 435 190
BsmBI CGTCTCn'nnnn_
Cuts at: 775 775
Size: 4742
Bsp1286I G_dGCh'C
Cuts at: 138 224 359 383 430 437 525 761 844
Size: 86 135 24 47 7 88 236 83
Cuts at: 844 902 1011 1321 1357 1369 1420 1729 1756
Size: 58 109 310 36 12 51 309 27
Cuts at: 1756 2367 2460 2534 2724 2786 3584 138
Size: 611 93 74 190 62 798 1296
Fragments arranged by size:
1296 798 611 310 309 236 190 135
109 93 88 86 83 74 62 58
51 47 36 27 24 12 7
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 347 1691 1792 2006 2308 2689 347
Size: 1344 101 214 302 381 2400
Fragments arranged by size:
2400 1344 381 302 214 101
BsrBI CCG'CTC
Cuts at: 50 1380 1511 3034 3088 4572 4737 50
Size: 1330 131 1523 54 1484 165 55
Fragments arranged by size:
1523 1484 1330 165 131 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 249 298 908 2740 2921 249
Size: 49 610 1832 181 2070
Fragments arranged by size:
2070 1832 610 181 49
BssHII G'CGCG_C
Cuts at: 809 809
Size: 4742
BssSI C'ACGA_G
Cuts at: 895 3013 3721 895
Size: 2118 708 1916
Fragments arranged by size:
2118 1916 708
BstAPI GCAn_nnn'nTGC
Cuts at: 354 795 1191 2119 354
Size: 441 396 928 2977
Fragments arranged by size:
2977 928 441 396
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstEII G'GTnAC_C
Cuts at: 168 1443 168
Size: 1275 3467
BstXI CCAn_nnnn'nTGG
Cuts at: 291 291
Size: 4742
BstYI r'GATC_y
Cuts at: 1 692 746 1472 1767 2234 2257 2592 2838
Size: 691 54 726 295 467 23 335 246
Cuts at: 2838 3144 3156 3242 4294 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 726 691 467 449 335 306 295
246 86 54 23 12
BtgI C'CryG_G
Cuts at: 284 606 1397 1665 284
Size: 322 791 268 3361
Fragments arranged by size:
3361 791 322 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1260 1601 2692 2873 3036 1260
Size: 341 1091 181 163 2966
Fragments arranged by size:
2966 1091 341 181 163
BtsI GCAGTG_nn'
Cuts at: 1985 1985
Size: 4742
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3792 2448
Size: 1344 3398
EaeI y'GGCC_r
Cuts at: 296 300 401 623 1631 2327 2501 2892 2919
Size: 4 101 222 1008 696 174 391 27
Cuts at: 2919 4264 296
Size: 1345 774
Fragments arranged by size:
1345 1008 774 696 391 222 174 101
27 4
EagI C'GGCC_G
Cuts at: 623 2327 623
Size: 1704 3038
EarI CTCTTCn'nnn_
Cuts at: 922 946 997 1613 2765 2975 922
Size: 24 51 616 1152 210 2689
Fragments arranged by size:
2689 1152 616 210 51 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1719 2950 3692 3838 4552 1719
Size: 1231 742 146 714 1909
Fragments arranged by size:
1909 1231 742 714 146
EcoICRI GAG'CTC
Cuts at: 1754 1754
Size: 4742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 235 277 364 565 709 946 1069 1249
Size: 93 42 87 201 144 237 123 180
Cuts at: 1249 1264 1333 2567 2999 3352 4014 142
Size: 15 69 1234 432 353 662 870
Fragments arranged by size:
1234 870 662 432 353 237 201 180
144 123 93 87 69 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1051 1645 1051
Size: 594 4148
EcoO109I rG'GnC_Cy
Cuts at: 236 373 540 936 957 1007 1384 1881 236
Size: 137 167 396 21 50 377 497 3097
Fragments arranged by size:
3097 497 396 377 167 137 50 21
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 2761 342
Size: 2419 2323
FseI GG_CCGG'CC
Cuts at: 302 302
Size: 4742
FspI TGC'GCA
Cuts at: 680 982 2523 680
Size: 302 1541 2899
Fragments arranged by size:
2899 1541 302
FspAI rTGC'GCAy
Cuts at: 680 680
Size: 4742
HaeII r_GCGC'y
Cuts at: 826 915 1346 1492 2176 2424 3654 4425 826
Size: 89 431 146 684 248 1230 771 1143
Fragments arranged by size:
1230 1143 771 684 431 248 146 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 691 723 2584 2614 2616 2646 691
Size: 32 1861 30 2 30 2787
Fragments arranged by size:
2787 1861 32 30 30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 911 1488 2172 2420 911
Size: 577 684 248 3233
Fragments arranged by size:
3233 684 577 248
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3501 3685 3501
Size: 184 4558
MscI TGG'CCA
Cuts at: 1633 2503 1633
Size: 870 3872
MslI CAynn'nnrTG
Cuts at: 1324 1360 2731 2858 1324
Size: 36 1371 127 3208
Fragments arranged by size:
3208 1371 127 36
MspA1I CmG'CkG
Cuts at: 116 213 608 792 1098 1137 1853 2167 2527
Size: 97 395 184 306 39 716 314 360
Cuts at: 2527 3311 3556 116
Size: 784 245 1302
Fragments arranged by size:
1302 784 716 395 360 314 306 245
184 97 39
NaeI GCC'GGC
Cuts at: 251 300 2923 251
Size: 49 2623 2070
Fragments arranged by size:
2623 2070 49
NarI GG'CG_CC
Cuts at: 912 1489 2173 2421 912
Size: 577 684 248 3233
Fragments arranged by size:
3233 684 577 248
NcoI C'CATG_G
Cuts at: 284 1397 284
Size: 1113 3629
NgoMIV G'CCGG_C
Cuts at: 249 298 2921 249
Size: 49 2623 2070
Fragments arranged by size:
2623 2070 49
NspI r_CATG'y
Cuts at: 1608 1795 2295 3898 1608
Size: 187 500 1603 2452
Fragments arranged by size:
2452 1603 500 187
PasI CC'CwG_GG
Cuts at: 1156 1156
Size: 4742
PciI A'CATG_T
Cuts at: 3894 3894
Size: 4742
PflMI CCAn_nnn'nTGG
Cuts at: 1529 1529
Size: 4742
PfoI T'CCnGG_A
Cuts at: 542 542
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 540 936 1384 540
Size: 396 448 3898
Fragments arranged by size:
3898 448 396
PspOMI G'GGCC_C
Cuts at: 1007 1365 1007
Size: 358 4384
PstI C_TGCA'G
Cuts at: 1789 2474 1789
Size: 685 4057
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 116 792 1098 1137 2167 2527 116
Size: 676 306 39 1030 360 2331
Fragments arranged by size:
2331 1030 676 360 306 39
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1756 1756
Size: 4742
SacII CC_GC'GG
Cuts at: 609 609
Size: 4742
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SexAI A'CCwGG_T
Cuts at: 995 995
Size: 4742
SfcI C'TryA_G
Cuts at: 33 1785 2470 3438 3629 33
Size: 1752 685 968 191 1146
Fragments arranged by size:
1752 1146 968 685 191
SfoI GGC'GCC
Cuts at: 913 1490 2174 2422 913
Size: 577 684 248 3233
Fragments arranged by size:
3233 684 577 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 572 1764 572
Size: 1192 3550
SmlI C'TyrA_G
Cuts at: 181 1897 2812 3526 3788 181
Size: 1716 915 714 262 1135
Fragments arranged by size:
1716 1135 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1608 1795 2295 1608
Size: 187 500 4055
Fragments arranged by size:
4055 500 187
StyI C'CwwG_G
Cuts at: 284 1397 1448 1517 1876 2230 284
Size: 1113 51 69 359 354 2796
Fragments arranged by size:
2796 1113 359 354 69 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1372 1428 2325 2458 2917 3126 1372
Size: 56 897 133 459 209 2988
Fragments arranged by size:
2988 897 459 209 133 56
TatI w'GTAC_w
Cuts at: 305 4257 305
Size: 3952 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1711 1743 4259 4291 1711
Size: 32 2516 32 2162
Fragments arranged by size:
2516 2162 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 1530 1560 2237 291
Size: 1239 30 677 2796
Fragments arranged by size:
2796 1239 677 30
XmaI C'CCGG_G
Cuts at: 570 1762 570
Size: 1192 3550
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsiWI
BsmI BspEI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI