Restriction map for
pF1KB5511
(Circular) (Six-base) MAPSORT of: pF1KB5511.seq Check: 1610 from: 1 to: 5294
pF1KB5511 5294 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 313 1661 313
Size: 1348 3946
AatII G_ACGT'C
Cuts at: 319 319
Size: 5294
AccI GT'mk_AC
Cuts at: 2332 2332
Size: 5294
Acc65I G'GTAC_C
Cuts at: 2310 2310
Size: 5294
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 469 1227 3119 3551 3904 4566 370
Size: 99 758 1892 432 353 662 1098
Fragments arranged by size:
1892 1098 758 662 432 353 99
AfeI AGC'GCT
Cuts at: 244 244
Size: 5294
AflIII A'CryG_T
Cuts at: 2646 4446 4727 2646
Size: 1800 281 3213
Fragments arranged by size:
3213 1800 281
AleI CACnn'nnGTG
Cuts at: 1795 1795
Size: 5294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1227 1507 1227
Size: 280 5014
AlwNI CAG_nnn'CTG
Cuts at: 735 2599 4037 735
Size: 1864 1438 1992
Fragments arranged by size:
1992 1864 1438
ApaI G_GGCC'C
Cuts at: 437 1162 2013 437
Size: 725 851 3718
Fragments arranged by size:
3718 851 725
ApaLI G'TGCA_C
Cuts at: 4132 4132
Size: 5294
ApoI r'AATT_y
Cuts at: 1948 2298 4647 4903 1948
Size: 350 2349 256 2339
Fragments arranged by size:
2349 2339 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 437 1687 2314 437
Size: 1250 627 3417
Fragments arranged by size:
3417 1250 627
AvrII C'CTAG_G
Cuts at: 285 285
Size: 5294
BamHI G'GATC_C
Cuts at: 2319 2319
Size: 5294
BanI G'GyrC_C
Cuts at: 2254 2310 2724 2972 3007 2254
Size: 56 414 248 35 4541
Fragments arranged by size:
4541 414 248 56 35
BanII G_rGCy'C
Cuts at: 437 446 500 568 1162 1277 1371 2013 2181
Size: 9 54 68 594 115 94 642 168
Cuts at: 2181 2240 2308 3338 437
Size: 59 68 1030 2393
Fragments arranged by size:
2393 1030 642 594 168 115 94 68
68 59 54 9
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 474 474
Size: 5294
BbvCI CC'TCA_GC
Cuts at: 491 598 491
Size: 107 5187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5260
BciVI GTATCCnnnnn_n'
Cuts at: 542 3192 4248 5294 542
Size: 2650 1056 1046 542
Fragments arranged by size:
2650 1056 1046 542
BclI T'GATC_A
Cuts at: 1475 2814 1475
Size: 1339 3955
BglI GCCn_nnn'nGGC
Cuts at: 941 941
Size: 5294
BglII A'GATC_T
Cuts at: 662 1515 2809 4846 662
Size: 853 1294 2037 1110
Fragments arranged by size:
2037 1294 1110 853
BlpI GC'TnA_GC
Cuts at: 246 2406 246
Size: 2160 3134
Bme1580I G_kGCm'C
Cuts at: 437 1162 1454 1491 2013 2257 2919 3012 4136
Size: 725 292 37 522 244 662 93 1124
Cuts at: 4136 437
Size: 1595
Fragments arranged by size:
1595 1124 725 662 522 292 244 93
37
BmgBI CAC'GTC
Cuts at: 417 1260 417
Size: 843 4451
BmrI ACTGGGnnnn_n'
Cuts at: 2007 2506 2587 2922 5066 2007
Size: 499 81 335 2144 2235
Fragments arranged by size:
2235 2144 499 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2076 3661 2076
Size: 1585 3709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 541 590 1978 382
Size: 159 49 1388 3698
Fragments arranged by size:
3698 1388 159 49
Bpu10I CC'TnA_GC
Cuts at: 491 598 1100 491
Size: 107 502 4685
Fragments arranged by size:
4685 502 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 922 2470 3349 4063 4361 748
Size: 174 1548 879 714 298 1681
Fragments arranged by size:
1681 1548 879 714 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 211 517 1850 4998 211
Size: 306 1333 3148 507
Fragments arranged by size:
3148 1333 507 306
BsaAI yAC'GTr
Cuts at: 1410 2647 1410
Size: 1237 4057
BsaBI GATnn'nnATC
Cuts at: 6 697 2808 4845 6
Size: 691 2111 2037 455
Fragments arranged by size:
2111 2037 691 455
BsaHI Gr'CG_yC
Cuts at: 170 316 1756 2725 2973 3675 5171 170
Size: 146 1440 969 248 702 1496 293
Fragments arranged by size:
1496 1440 969 702 293 248 146
BsaWI w'CCGG_w
Cuts at: 534 2483 3004 3682 4093 4240 4514 534
Size: 1949 521 678 411 147 274 1314
Fragments arranged by size:
1949 1314 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 514 544 514
Size: 30 5264
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 1834 508
Size: 1326 3968
BseYI C'CCAG_C
Cuts at: 135 2719 3477 4142 4550 135
Size: 2584 758 665 408 879
Fragments arranged by size:
2584 879 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 1215 1397 1441 1639 891
Size: 324 182 44 198 4546
Fragments arranged by size:
4546 324 198 182 44
BsiEI CG_ry'CG
Cuts at: 104 2836 2882 4112 104
Size: 2732 46 1230 1286
Fragments arranged by size:
2732 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 446 500 764 1832 2308 3086 3276 4136 446
Size: 54 264 1068 476 778 190 860 1604
Fragments arranged by size:
1604 1068 860 778 476 264 190 54
BsmI GAATG_Cn'
Cuts at: 1185 1185
Size: 5294
BsmBI CGTCTCn'nnnn_
Cuts at: 1747 1747
Size: 5294
Bsp1286I G_dGCh'C
Cuts at: 437 446 500 568 764 1162 1277 1371 1454
Size: 9 54 68 196 398 115 94 83
Cuts at: 1454 1491 1832 2013 2181 2240 2257 2308 2919
Size: 37 341 181 168 59 17 51 611
Cuts at: 2919 3012 3086 3276 3338 4136 437
Size: 93 74 190 62 798 1595
Fragments arranged by size:
1595 798 611 398 341 196 190 181
168 115 94 93 83 74 68 62
59 54 51 37 17 9
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 5294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 313 1661 2344 2558 2860 3241 313
Size: 1348 683 214 302 381 2366
Fragments arranged by size:
2366 1348 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1935 3586 3640 5124 5289 50
Size: 1885 1651 54 1484 165 55
Fragments arranged by size:
1885 1651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1316 1964 3206 1316
Size: 648 1242 3404
Fragments arranged by size:
3404 1242 648
BsrFI r'CCGG_y
Cuts at: 1214 1601 3292 3473 1214
Size: 387 1691 181 3035
Fragments arranged by size:
3035 1691 387 181
BssSI C'ACGA_G
Cuts at: 1081 3565 4273 1081
Size: 2484 708 2102
Fragments arranged by size:
2484 2102 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2671 2671
Size: 5294
BstBI TT'CG_AA
Cuts at: 896 3655 896
Size: 2759 2535
BstXI CCAn_nnnn'nTGG
Cuts at: 280 972 1197 1734 280
Size: 692 225 537 3840
Fragments arranged by size:
3840 692 537 225
BstYI r'GATC_y
Cuts at: 1 269 384 662 1515 2319 2786 2809 3144
Size: 268 115 278 853 804 467 23 335
Cuts at: 3144 3390 3696 3708 3794 4846 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 853 804 467 449 335 306 278
268 246 115 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1163 1163
Size: 5294
BtgI C'CryG_G
Cuts at: 828 965 828
Size: 137 5157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2088 2250 3244 3425 3588 2088
Size: 162 994 181 163 3794
Fragments arranged by size:
3794 994 181 163 162
BtsI GCAGTG_nn'
Cuts at: 1491 2537 1491
Size: 1046 4248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 987 1022 987
Size: 35 5259
DraI TTT'AAA
Cuts at: 2293 2293
Size: 5294
DraIII CAC_nnn'GTG
Cuts at: 914 914
Size: 5294
DrdI GACnn_nn'nnGTC
Cuts at: 3000 4344 3000
Size: 1344 3950
EaeI y'GGCC_r
Cuts at: 152 206 611 1614 1836 2879 3053 3444 3471
Size: 54 405 1003 222 1043 174 391 27
Cuts at: 3471 4816 152
Size: 1345 630
Fragments arranged by size:
1345 1043 1003 630 405 391 222 174
54 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 403 676 1103 1525 3317 3527 403
Size: 273 427 422 1792 210 2170
Fragments arranged by size:
2170 1792 427 422 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3502 4244 4390 5104 3502
Size: 742 146 714 3692
Fragments arranged by size:
3692 742 714 146
EcoICRI GAG'CTC
Cuts at: 444 498 2306 444
Size: 54 1808 3432
Fragments arranged by size:
3432 1808 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 382 469 541 590 1227 1978 3119 3551
Size: 12 87 72 49 637 751 1141 432
Cuts at: 3551 3904 4566 370
Size: 353 662 1098
Fragments arranged by size:
1141 1098 751 662 637 432 353 87
72 49 12
EcoNI CCTnn'n_nnAGG
Cuts at: 289 1669 289
Size: 1380 3914
EcoO109I rG'GnC_Cy
Cuts at: 433 434 1159 2009 2433 433
Size: 1 725 850 424 3294
Fragments arranged by size:
3294 850 725 424 1
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 684 3313 684
Size: 2629 2665
FspI TGC'GCA
Cuts at: 931 3075 931
Size: 2144 3150
HaeII r_GCGC'y
Cuts at: 246 280 2728 2976 4206 4977 246
Size: 34 2448 248 1230 771 563
Fragments arranged by size:
2448 1230 771 563 248 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 516 548 563 595 1055 1087 1126 1158 1492
Size: 32 15 32 460 32 39 32 334
Cuts at: 1492 1524 3136 3166 3168 3198 516
Size: 32 1612 30 2 30 2612
Fragments arranged by size:
2612 1612 460 334 39 32 32 32
32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 2333 2333
Size: 5294
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 2314 2314
Size: 5294
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1323 4053 4237 1323
Size: 2730 184 2380
Fragments arranged by size:
2730 2380 184
MscI TGG'CCA
Cuts at: 208 1616 1838 3055 208
Size: 1408 222 1217 2447
Fragments arranged by size:
2447 1408 1217 222
MslI CAynn'nnrTG
Cuts at: 1795 1912 2078 2228 3283 3410 1795
Size: 117 166 150 1055 127 3679
Fragments arranged by size:
3679 1055 166 150 127 117
MspA1I CmG'CkG
Cuts at: 648 716 1026 1229 1326 2405 2719 3079 3863
Size: 68 310 203 97 1079 314 360 784
Cuts at: 3863 4108 648
Size: 245 1834
Fragments arranged by size:
1834 1079 784 360 314 310 245 203
97 68
NaeI GCC'GGC
Cuts at: 1216 3475 1216
Size: 2259 3035
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NcoI C'CATG_G
Cuts at: 828 828
Size: 5294
NgoMIV G'CCGG_C
Cuts at: 1214 3473 1214
Size: 2259 3035
NspI r_CATG'y
Cuts at: 1062 2347 2847 4450 1062
Size: 1285 500 1603 1906
Fragments arranged by size:
1906 1603 1285 500
PciI A'CATG_T
Cuts at: 4446 4446
Size: 5294
PflMI CCAn_nnn'nTGG
Cuts at: 735 735
Size: 5294
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 5262
PspOMI G'GGCC_C
Cuts at: 433 1158 2009 433
Size: 725 851 3718
Fragments arranged by size:
3718 851 725
PstI C_TGCA'G
Cuts at: 854 1508 2341 3026 854
Size: 654 833 685 3122
Fragments arranged by size:
3122 833 685 654
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5294
PvuII CAG'CTG
Cuts at: 648 1229 2719 3079 648
Size: 581 1490 360 2863
Fragments arranged by size:
2863 1490 581 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 446 500 2308 446
Size: 54 1808 3432
Fragments arranged by size:
3432 1808 54
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5294
SapI GCTCTTCn'nnn_
Cuts at: 3317 3527 3317
Size: 210 5084
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 850 1504 2337 3022 3990 4181 33
Size: 817 654 833 685 968 191 1146
Fragments arranged by size:
1146 968 833 817 685 654 191
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 439 2316 439
Size: 1877 3417
SmlI C'TyrA_G
Cuts at: 763 937 2449 3364 4078 4340 763
Size: 174 1512 915 714 262 1717
Fragments arranged by size:
1717 1512 915 714 262 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4794
StyI C'CwwG_G
Cuts at: 285 746 828 1611 2182 2428 2782 285
Size: 461 82 783 571 246 354 2797
Fragments arranged by size:
2797 783 571 461 354 246 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 1285 1663 2877 3010 3469 3678 235
Size: 1050 378 1214 133 459 209 1851
Fragments arranged by size:
1851 1214 1050 459 378 209 133
TatI w'GTAC_w
Cuts at: 1706 1961 4809 1706
Size: 255 2848 2191
Fragments arranged by size:
2848 2191 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4811 4843 4811
Size: 32 5262
Tth111I GACn'n_nGTC
Cuts at: 3091 3091
Size: 5294
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 474 534 666 736 2189 2789 474
Size: 60 132 70 1453 600 2979
Fragments arranged by size:
2979 1453 600 132 70 60
XmaI C'CCGG_G
Cuts at: 437 2314 437
Size: 1877 3417
XmnI GAAnn'nnTTC
Cuts at: 454 454
Size: 5294
ZraI GAC'GTC
Cuts at: 317 317
Size: 5294
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI AscI BaeI BfrBI BmtI
BsiWI BspEI BsrGI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI