Restriction map for
pF1KB5513
(Circular) (Six-base) MAPSORT of: pF1KB5513.seq Check: 7639 from: 1 to: 5213
pF1KB5513 5213 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 2251 2251
Size: 5213
Acc65I G'GTAC_C
Cuts at: 1966 2229 1966
Size: 263 4950
AclI AA'CG_TT
Cuts at: 5053 5053
Size: 5213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1806 1909 3038 3470 4217 4485 1806
Size: 103 1129 432 747 268 2534
Fragments arranged by size:
2534 1129 747 432 268 103
AfeI AGC'GCT
Cuts at: 1489 1489
Size: 5213
AflIII A'CryG_T
Cuts at: 893 2565 3669 4646 893
Size: 1672 1104 977 1460
Fragments arranged by size:
1672 1460 1104 977
AgeI A'CCGG_T
Cuts at: 3601 3601
Size: 5213
AlwNI CAG_nnn'CTG
Cuts at: 879 948 1269 2518 4085 879
Size: 69 321 1249 1567 2007
Fragments arranged by size:
2007 1567 1249 321 69
ApaI G_GGCC'C
Cuts at: 302 302
Size: 5213
ApaLI G'TGCA_C
Cuts at: 3983 3983
Size: 5213
ApoI r'AATT_y
Cuts at: 1075 1934 1974 2217 4566 4822 1075
Size: 859 40 243 2349 256 1466
Fragments arranged by size:
2349 1466 859 256 243 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 5213
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
AvaI C'yCGr_G
Cuts at: 317 473 2149 2233 317
Size: 156 1676 84 3297
Fragments arranged by size:
3297 1676 156 84
AvrII C'CTAG_G
Cuts at: 411 411
Size: 5213
BamHI G'GATC_C
Cuts at: 2145 2238 2145
Size: 93 5120
BanI G'GyrC_C
Cuts at: 700 1966 2229 2643 2891 2926 700
Size: 1266 263 414 248 35 2987
Fragments arranged by size:
2987 1266 414 263 248 35
BanII G_rGCy'C
Cuts at: 166 302 411 1213 1340 2174 2227 3257 166
Size: 136 109 802 127 834 53 1030 2122
Fragments arranged by size:
2122 1030 834 802 136 127 109 53
BbeI G_GCGC'C
Cuts at: 2647 2895 2647
Size: 248 4965
BbsI GAAGACnn'nnnn_
Cuts at: 728 728
Size: 5213
BbvCI CC'TCA_GC
Cuts at: 1450 1450
Size: 5213
BciVI GTATCCnnnnn_n'
Cuts at: 3111 3872 5213 3111
Size: 761 1341 3111
Fragments arranged by size:
3111 1341 761
BclI T'GATC_A
Cuts at: 1271 2733 1271
Size: 1462 3751
BglI GCCn_nnn'nGGC
Cuts at: 130 1729 130
Size: 1599 3614
BglII A'GATC_T
Cuts at: 347 1124 1748 2728 4765 347
Size: 777 624 980 2037 795
Fragments arranged by size:
2037 980 795 777 624
BlpI GC'TnA_GC
Cuts at: 2325 2325
Size: 5213
Bme1580I G_kGCm'C
Cuts at: 302 893 2838 2931 3987 302
Size: 591 1945 93 1056 1528
Fragments arranged by size:
1945 1528 1056 591 93
BmrI ACTGGGnnnn_n'
Cuts at: 357 1719 2425 2506 2841 4985 357
Size: 1362 706 81 335 2144 585
Fragments arranged by size:
2144 1362 706 585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 5213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 291 657 670 1141 1221 1300 247
Size: 44 366 13 471 80 79 4160
Fragments arranged by size:
4160 471 366 80 79 44 13
Bpu10I CC'TnA_GC
Cuts at: 1450 1450
Size: 5213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 3268 3760 4058 2389
Size: 879 492 298 3544
Fragments arranged by size:
3544 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4917 4917
Size: 5213
BsaAI yAC'GTr
Cuts at: 2566 2566
Size: 5213
BsaBI GATnn'nnATC
Cuts at: 6 955 2727 4764 6
Size: 949 1772 2037 455
Fragments arranged by size:
2037 1772 949 455
BsaHI Gr'CG_yC
Cuts at: 2644 2892 3594 5090 2644
Size: 248 702 1496 2767
Fragments arranged by size:
2767 1496 702 248
BsaWI w'CCGG_w
Cuts at: 730 907 2402 2923 3601 3875 4022 4433 730
Size: 177 1495 521 678 274 147 411 1510
Fragments arranged by size:
1510 1495 678 521 411 274 177 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 392 931 1051 1297 1300 1315 1435 392
Size: 539 120 246 3 15 120 4170
Fragments arranged by size:
4170 539 246 120 120 15 3
BseYI C'CCAG_C
Cuts at: 159 1881 2638 3396 3973 4469 159
Size: 1722 757 758 577 496 903
Fragments arranged by size:
1722 903 758 757 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1305
Size: 5213
BsiEI CG_ry'CG
Cuts at: 104 152 2755 2801 4009 104
Size: 48 2603 46 1208 1308
Fragments arranged by size:
2603 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 1523 2174 2227 3005 3195 3987 1523
Size: 651 53 778 190 792 2749
Fragments arranged by size:
2749 792 778 651 190 53
BsmI GAATG_Cn'
Cuts at: 612 612
Size: 5213
Bsp1286I G_dGCh'C
Cuts at: 166 302 411 893 1213 1340 1523 2174 2227
Size: 136 109 482 320 127 183 651 53
Cuts at: 2227 2838 2931 3005 3195 3257 3987 166
Size: 611 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 651 611 482 320 190 183
136 127 109 93 74 62 53
BspEI T'CCGG_A
Cuts at: 4433 4433
Size: 5213
BspHI T'CATG_A
Cuts at: 4389 4389
Size: 5213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1999 2263 2477 2779 3160 1999
Size: 264 214 302 381 4052
Fragments arranged by size:
4052 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 1316 3505 3559 5043 5208 50
Size: 1266 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 1266 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 737 3125 437
Size: 300 2388 2525
Fragments arranged by size:
2525 2388 300
BsrFI r'CCGG_y
Cuts at: 3211 3392 3601 3211
Size: 181 209 4823
Fragments arranged by size:
4823 209 181
BssSI C'ACGA_G
Cuts at: 169 1558 3484 3842 169
Size: 1389 1926 358 1540
Fragments arranged by size:
1926 1540 1389 358
BstAPI GCAn_nnn'nTGC
Cuts at: 439 2590 439
Size: 2151 3062
BstBI TT'CG_AA
Cuts at: 719 1574 3574 719
Size: 855 2000 2358
Fragments arranged by size:
2358 2000 855
BstEII G'GTnAC_C
Cuts at: 903 903
Size: 5213
BstYI r'GATC_y
Cuts at: 1 347 518 1124 1748 2145 2238 2705 2728
Size: 346 171 606 624 397 93 467 23
Cuts at: 2728 3063 3309 4321 4407 4419 4765 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 624 606 467 449 397 346 346
335 246 171 93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1102 1102
Size: 5213
BtgI C'CryG_G
Cuts at: 107 341 848 1217 107
Size: 234 507 369 4103
Fragments arranged by size:
4103 507 369 234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 102 3163 3344 3507 102
Size: 3061 181 163 1808
Fragments arranged by size:
3061 1808 181 163
BtsI GCAGTG_nn'
Cuts at: 2456 2456
Size: 5213
ClaI AT'CG_AT
Cuts at: 2078 2078
Size: 5213
DraI TTT'AAA
Cuts at: 2212 2212
Size: 5213
DrdI GACnn_nn'nnGTC
Cuts at: 2919 3777 2919
Size: 858 4355
EaeI y'GGCC_r
Cuts at: 122 149 263 2798 2972 3363 3390 4735 122
Size: 27 114 2535 174 391 27 1345 600
Fragments arranged by size:
2535 1345 600 391 174 114 27 27
EagI C'GGCC_G
Cuts at: 149 2798 149
Size: 2649 2564
EarI CTCTTCn'nnn_
Cuts at: 247 1664 3236 3446 247
Size: 1417 1572 210 2014
Fragments arranged by size:
2014 1572 1417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3421 3731 3877 5023 3421
Size: 310 146 1146 3611
Fragments arranged by size:
3611 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2172 2225 2172
Size: 53 5160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 291 657 670 1141 1221 1300 1806 1909
Size: 44 366 13 471 80 79 506 103
Cuts at: 1909 3038 3470 4217 4485 247
Size: 1129 432 747 268 975
Fragments arranged by size:
1129 975 747 506 471 432 366 268
103 80 79 44 13
EcoNI CCTnn'n_nnAGG
Cuts at: 193 1513 193
Size: 1320 3893
EcoO109I rG'GnC_Cy
Cuts at: 249 298 299 2352 249
Size: 49 1 2053 3110
Fragments arranged by size:
3110 2053 49 1
EcoRI G'AATT_C
Cuts at: 1075 1974 2217 1075
Size: 899 243 4071
Fragments arranged by size:
4071 899 243
EcoRV GAT'ATC
Cuts at: 1806 1806
Size: 5213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 5213
FspI TGC'GCA
Cuts at: 2994 2994
Size: 5213
HaeII r_GCGC'y
Cuts at: 211 339 644 848 1491 1800 2647 2895 3917
Size: 128 305 204 643 309 847 248 1022
Cuts at: 3917 4896 211
Size: 979 528
Fragments arranged by size:
1022 979 847 643 528 309 305 248
204 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1245 1277 1795 1827 2010 2042 2167 2199 3055
Size: 32 518 32 183 32 125 32 856
Cuts at: 3055 3085 3087 3117 1245
Size: 30 2 30 3341
Fragments arranged by size:
3341 856 518 183 125 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1851 1956 2029 2252 1851
Size: 105 73 223 4812
Fragments arranged by size:
4812 223 105 73
KasI G'GCGC_C
Cuts at: 2643 2891 2643
Size: 248 4965
KpnI G_GTAC'C
Cuts at: 1970 2233 1970
Size: 263 4950
MluI A'CGCG_T
Cuts at: 893 4646 893
Size: 3753 1460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3884 4068 3884
Size: 184 5029
MscI TGG'CCA
Cuts at: 2974 2974
Size: 5213
MslI CAynn'nnrTG
Cuts at: 1067 1186 1558 3202 3329 1067
Size: 119 372 1644 127 2951
Fragments arranged by size:
2951 1644 372 127 119
MspA1I CmG'CkG
Cuts at: 850 1219 2142 2324 2638 2998 4011 4256 850
Size: 369 923 182 314 360 1013 245 1807
Fragments arranged by size:
1807 1013 923 369 360 314 245 182
NaeI GCC'GGC
Cuts at: 3394 3394
Size: 5213
NarI GG'CG_CC
Cuts at: 2644 2892 2644
Size: 248 4965
NcoI C'CATG_G
Cuts at: 107 107
Size: 5213
NgoMIV G'CCGG_C
Cuts at: 3392 3392
Size: 5213
NspI r_CATG'y
Cuts at: 1386 1838 1857 2266 2766 3673 1386
Size: 452 19 409 500 907 2926
Fragments arranged by size:
2926 907 500 452 409 19
PasI CC'CwG_GG
Cuts at: 279 968 279
Size: 689 4524
PciI A'CATG_T
Cuts at: 3669 3669
Size: 5213
PfoI T'CCnGG_A
Cuts at: 245 500 1511 245
Size: 255 1011 3947
Fragments arranged by size:
3947 1011 255
PmeI GTTT'AAAC
Cuts at: 2212 2212
Size: 5213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 5181
PpuMI rG'GwC_Cy
Cuts at: 249 249
Size: 5213
PspOMI G'GGCC_C
Cuts at: 298 298
Size: 5213
PspXI vC'TCGA_Gb
Cuts at: 473 473
Size: 5213
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1249 1281 1249
Size: 32 5181
PstI C_TGCA'G
Cuts at: 1769 2260 2945 1769
Size: 491 685 4037
Fragments arranged by size:
4037 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5213
PvuII CAG'CTG
Cuts at: 2142 2638 2998 2142
Size: 496 360 4357
Fragments arranged by size:
4357 496 360
RsrII CG'GwC_CG
Cuts at: 3408 3408
Size: 5213
SacI G_AGCT'C
Cuts at: 2174 2227 2174
Size: 53 5160
SacII CC_GC'GG
Cuts at: 851 1220 851
Size: 369 4844
SalI G'TCGA_C
Cuts at: 2250 2250
Size: 5213
SapI GCTCTTCn'nnn_
Cuts at: 247 3236 3446 247
Size: 2989 210 2014
Fragments arranged by size:
2989 2014 210
SbfI CC_TGCA'GG
Cuts at: 2260 2260
Size: 5213
ScaI AGT'ACT
Cuts at: 2204 4730 2204
Size: 2526 2687
SfcI C'TryA_G
Cuts at: 33 940 1025 1765 2256 2941 3934 4125 33
Size: 907 85 740 491 685 993 191 1121
Fragments arranged by size:
1121 993 907 740 685 491 191 85
SfoI GGC'GCC
Cuts at: 2645 2893 2645
Size: 248 4965
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5213
SmaI CCC'GGG
Cuts at: 319 2235 319
Size: 1916 3297
SmlI C'TyrA_G
Cuts at: 473 2368 3283 3775 4037 473
Size: 1895 915 492 262 1649
Fragments arranged by size:
1895 1649 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5213
SphI G_CATG'C
Cuts at: 2266 2766 2266
Size: 500 4713
StyI C'CwwG_G
Cuts at: 107 402 411 587 819 2347 2701 107
Size: 295 9 176 232 1528 354 2619
Fragments arranged by size:
2619 1528 354 295 232 176 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 543 891 2796 2929 3388 3597 543
Size: 348 1905 133 459 209 2159
Fragments arranged by size:
2159 1905 459 348 209 133
TatI w'GTAC_w
Cuts at: 1550 2202 4728 1550
Size: 652 2526 2035
Fragments arranged by size:
2526 2035 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4730 4762 4730
Size: 32 5181
Tth111I GACn'n_nGTC
Cuts at: 3010 3010
Size: 5213
XbaI T'CTAG_A
Cuts at: 2244 2244
Size: 5213
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 370 2708 370
Size: 2338 2875
XhoI C'TCGA_G
Cuts at: 473 473
Size: 5213
XmaI C'CCGG_G
Cuts at: 317 2233 317
Size: 1916 3297
XmnI GAAnn'nnTTC
Cuts at: 1246 1894 1246
Size: 648 4565
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI