Restriction map for
pF1KB5521
(Circular) (Six-base) MAPSORT of: pF1KB5521.seq Check: 8244 from: 1 to: 4457
pF1KB5521 4457 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1495 1495
Size: 4457
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 1033 2282 2714 3461 3729 846
Size: 187 1249 432 747 268 1574
Fragments arranged by size:
1574 1249 747 432 268 187
AfeI AGC'GCT
Cuts at: 897 897
Size: 4457
AflIII A'CryG_T
Cuts at: 240 1809 2913 3890 240
Size: 1569 1104 977 807
Fragments arranged by size:
1569 1104 977 807
AgeI A'CCGG_T
Cuts at: 2845 2845
Size: 4457
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 616
Size: 32 4425
AlwNI CAG_nnn'CTG
Cuts at: 1762 3329 1762
Size: 1567 2890
ApaLI G'TGCA_C
Cuts at: 3227 3227
Size: 4457
ApoI r'AATT_y
Cuts at: 709 1002 1025 1461 3810 4066 709
Size: 293 23 436 2349 256 1100
Fragments arranged by size:
2349 1100 436 293 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 1477 1477
Size: 4457
AvrII C'CTAG_G
Cuts at: 373 373
Size: 4457
BamHI G'GATC_C
Cuts at: 942 1482 942
Size: 540 3917
BanI G'GyrC_C
Cuts at: 1473 1887 2135 2170 1473
Size: 414 248 35 3760
Fragments arranged by size:
3760 414 248 35
BanII G_rGCy'C
Cuts at: 616 821 1451 1471 2501 616
Size: 205 630 20 1030 2572
Fragments arranged by size:
2572 1030 630 205 20
BbeI G_GCGC'C
Cuts at: 1891 2139 1891
Size: 248 4209
BbsI GAAGACnn'nnnn_
Cuts at: 667 667
Size: 4457
BbvCI CC'TCA_GC
Cuts at: 899 899
Size: 4457
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3116 4457 2355
Size: 761 1341 2355
Fragments arranged by size:
2355 1341 761
BclI T'GATC_A
Cuts at: 1977 1977
Size: 4457
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 1569 1569
Size: 4457
Bme1580I G_kGCm'C
Cuts at: 2082 2175 3231 2082
Size: 93 1056 3308
Fragments arranged by size:
3308 1056 93
BmgBI CAC'GTC
Cuts at: 872 872
Size: 4457
BmrI ACTGGGnnnn_n'
Cuts at: 1669 1750 2085 4229 1669
Size: 81 335 2144 1897
Fragments arranged by size:
2144 1897 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 2824 1206
Size: 1618 2839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 315
Size: 4457
Bpu10I CC'TnA_GC
Cuts at: 899 899
Size: 4457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 505 630 1446 1633 2512 3004 3302 161
Size: 344 125 816 187 879 492 298 1316
Fragments arranged by size:
1316 879 816 492 344 298 187 125
BsaI GGTCTCn'nnnn_
Cuts at: 293 4161 293
Size: 3868 589
BsaAI yAC'GTr
Cuts at: 1810 1810
Size: 4457
BsaBI GATnn'nnATC
Cuts at: 6 1971 4008 6
Size: 1965 2037 455
Fragments arranged by size:
2037 1965 455
BsaHI Gr'CG_yC
Cuts at: 1888 2136 2838 4334 1888
Size: 248 702 1496 2011
Fragments arranged by size:
2011 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3119 3266 3677 1646
Size: 521 678 274 147 411 2426
Fragments arranged by size:
2426 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 916 829
Size: 87 4370
BseYI C'CCAG_C
Cuts at: 650 892 1416 1882 2640 3217 3713 650
Size: 242 524 466 758 577 496 1394
Fragments arranged by size:
1394 758 577 524 496 466 242
BsiEI CG_ry'CG
Cuts at: 104 1138 1999 2045 3253 104
Size: 1034 861 46 1208 1308
Fragments arranged by size:
1308 1208 1034 861 46
BsiHKAI G_wGCw'C
Cuts at: 821 1471 2249 2439 3231 821
Size: 650 778 190 792 2047
Fragments arranged by size:
2047 792 778 650 190
Bsp1286I G_dGCh'C
Cuts at: 616 821 1451 1471 2082 2175 2249 2439 2501
Size: 205 630 20 611 93 74 190 62
Cuts at: 2501 3231 616
Size: 730 1842
Fragments arranged by size:
1842 730 630 611 205 190 93 74
62 20
BspEI T'CCGG_A
Cuts at: 3677 3677
Size: 4457
BspHI T'CATG_A
Cuts at: 219 3633 219
Size: 3414 1043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1507 1721 2023 2404 1507
Size: 214 302 381 3560
Fragments arranged by size:
3560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1044 2749 2803 4287 4452 50
Size: 994 1705 54 1484 165 55
Fragments arranged by size:
1705 1484 994 165 55 54
BsrDI GCAATG_nn'
Cuts at: 320 1192 2369 320
Size: 872 1177 2408
Fragments arranged by size:
2408 1177 872
BsrFI r'CCGG_y
Cuts at: 771 2455 2636 2845 771
Size: 1684 181 209 2383
Fragments arranged by size:
2383 1684 209 181
BssSI C'ACGA_G
Cuts at: 749 2728 3086 749
Size: 1979 358 2120
Fragments arranged by size:
2120 1979 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1834 1834
Size: 4457
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 700 700
Size: 4457
BstYI r'GATC_y
Cuts at: 1 489 942 1061 1482 1949 1972 2307 2553
Size: 488 453 119 421 467 23 335 246
Cuts at: 2553 3565 3651 3663 4009 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 488 467 453 449 421 346 335
246 119 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1037 1037
Size: 4457
BtgI C'CryG_G
Cuts at: 668 668
Size: 4457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 121 426 995 1700 121
Size: 305 569 705 2878
Fragments arranged by size:
2878 705 569 305
ClaI AT'CG_AT
Cuts at: 562 562
Size: 4457
DraI TTT'AAA
Cuts at: 1456 1456
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3021 2163
Size: 858 3599
EaeI y'GGCC_r
Cuts at: 639 2042 2216 2607 2634 3979 639
Size: 1403 174 391 27 1345 1117
Fragments arranged by size:
1403 1345 1117 391 174 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 159 598 806 1009 2480 2690 159
Size: 439 208 203 1471 210 1926
Fragments arranged by size:
1926 1471 439 210 208 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 531 2665 2975 3121 4267 531
Size: 2134 310 146 1146 721
Fragments arranged by size:
2134 1146 721 310 146
EcoICRI GAG'CTC
Cuts at: 819 1469 819
Size: 650 3807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 846 1033 2282 2714 3461 3729 315
Size: 531 187 1249 432 747 268 1043
Fragments arranged by size:
1249 1043 747 531 432 268 187
EcoNI CCTnn'n_nnAGG
Cuts at: 164 164
Size: 4457
EcoO109I rG'GnC_Cy
Cuts at: 181 1596 181
Size: 1415 3042
EcoRI G'AATT_C
Cuts at: 1002 1461 1002
Size: 459 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 624 2476 169
Size: 455 1852 2150
Fragments arranged by size:
2150 1852 455
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 899 1891 2139 3161 4140 899
Size: 992 248 1022 979 1216
Fragments arranged by size:
1216 1022 992 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2299 2329 2331 2361 2299
Size: 30 2 30 4395
Fragments arranged by size:
4395 30 30 2
HincII GTy'rAC
Cuts at: 1496 1496
Size: 4457
KasI G'GCGC_C
Cuts at: 1887 2135 1887
Size: 248 4209
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1304 3128 3312 1304
Size: 1824 184 2449
Fragments arranged by size:
2449 1824 184
MscI TGG'CCA
Cuts at: 641 2218 641
Size: 1577 2880
MslI CAynn'nnrTG
Cuts at: 218 673 704 1215 1318 2446 2573 218
Size: 455 31 511 103 1128 127 2102
Fragments arranged by size:
2102 1128 511 455 127 103 31
MspA1I CmG'CkG
Cuts at: 1392 1568 1882 2242 3255 3500 1392
Size: 176 314 360 1013 245 2349
Fragments arranged by size:
2349 1013 360 314 245 176
NaeI GCC'GGC
Cuts at: 773 2638 773
Size: 1865 2592
NarI GG'CG_CC
Cuts at: 1888 2136 1888
Size: 248 4209
NcoI C'CATG_G
Cuts at: 668 668
Size: 4457
NdeI CA'TA_TG
Cuts at: 1092 1359 1092
Size: 267 4190
NgoMIV G'CCGG_C
Cuts at: 771 2636 771
Size: 1865 2592
NspI r_CATG'y
Cuts at: 230 244 525 1510 2010 2917 230
Size: 14 281 985 500 907 1770
Fragments arranged by size:
1770 985 907 500 281 14
PciI A'CATG_T
Cuts at: 240 2913 240
Size: 2673 1784
PflMI CCAn_nnn'nTGG
Cuts at: 293 293
Size: 4457
PfoI T'CCnGG_A
Cuts at: 159 159
Size: 4457
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4425
PpuMI rG'GwC_Cy
Cuts at: 181 181
Size: 4457
PstI C_TGCA'G
Cuts at: 1277 1504 2189 1277
Size: 227 685 3545
Fragments arranged by size:
3545 685 227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 1882 2242 1882
Size: 360 4097
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 821 1471 821
Size: 650 3807
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 598 2480 2690 598
Size: 1882 210 2365
Fragments arranged by size:
2365 1882 210
SbfI CC_TGCA'GG
Cuts at: 1277 1504 1277
Size: 227 4230
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SfcI C'TryA_G
Cuts at: 33 1273 1500 2185 3178 3369 33
Size: 1240 227 685 993 191 1121
Fragments arranged by size:
1240 1121 993 685 227 191
SfoI GGC'GCC
Cuts at: 1889 2137 1889
Size: 248 4209
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 176 484 609 1425 1612 2527 3019 3281 176
Size: 308 125 816 187 915 492 262 1352
Fragments arranged by size:
1352 915 816 492 308 262 187 125
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1510 2010 1510
Size: 500 3957
SspI AAT'ATT
Cuts at: 445 445
Size: 4457
StyI C'CwwG_G
Cuts at: 137 373 668 1591 1945 137
Size: 236 295 923 354 2649
Fragments arranged by size:
2649 923 354 295 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2040 2173 2632 2841 2040
Size: 133 459 209 3656
Fragments arranged by size:
3656 459 209 133
TatI w'GTAC_w
Cuts at: 1251 1376 3972 1251
Size: 125 2596 1736
Fragments arranged by size:
2596 1736 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 927 959 1053 1085 3974 4006 927
Size: 32 94 32 2889 32 1378
Fragments arranged by size:
2889 1378 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1952 1952
Size: 4457
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
XmnI GAAnn'nnTTC
Cuts at: 1051 1051
Size: 4457
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
BaeI BcgI BfrBI BglI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI