Restriction map for
pF1KB5522
(Circular) (Six-base) MAPSORT of: pF1KB5522.seq Check: 4707 from: 1 to: 4259
pF1KB5522 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 2084 2516 2869 3531 963
Size: 1121 432 353 662 1691
Fragments arranged by size:
1691 1121 662 432 353
AfeI AGC'GCT
Cuts at: 532 532
Size: 4259
AflIII A'CryG_T
Cuts at: 1611 3411 3692 1611
Size: 1800 281 2178
Fragments arranged by size:
2178 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 605 1564 3002 605
Size: 959 1438 1862
Fragments arranged by size:
1862 1438 959
ApaI G_GGCC'C
Cuts at: 116 116
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3097 3097
Size: 4259
ApoI r'AATT_y
Cuts at: 331 1123 1153 1263 3612 3868 331
Size: 792 30 110 2349 256 722
Fragments arranged by size:
2349 792 722 256 110 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 1101 1279 1101
Size: 178 4081
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 915 1021 1275 1689 1937 1972 915
Size: 106 254 414 248 35 3202
Fragments arranged by size:
3202 414 254 248 106 35
BanII G_rGCy'C
Cuts at: 116 1273 2303 116
Size: 1157 1030 2072
Fragments arranged by size:
2072 1157 1030
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbsI GAAGACnn'nnnn_
Cuts at: 726 891 967 1220 726
Size: 165 76 253 3765
Fragments arranged by size:
3765 253 165 76
BciVI GTATCCnnnnn_n'
Cuts at: 2157 3213 4259 2157
Size: 1056 1046 2157
Fragments arranged by size:
2157 1056 1046
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 913 913
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1371 1371
Size: 4259
Bme1580I G_kGCm'C
Cuts at: 116 918 1102 1884 1977 3101 116
Size: 802 184 782 93 1124 1274
Fragments arranged by size:
1274 1124 802 782 184 93
BmrI ACTGGGnnnn_n'
Cuts at: 447 1471 1552 1887 4031 447
Size: 1024 81 335 2144 675
Fragments arranged by size:
2144 1024 675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4259
Bpu10I CC'TnA_GC
Cuts at: 892 892
Size: 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1435 2314 3028 3326 856
Size: 579 879 714 298 1789
Fragments arranged by size:
1789 879 714 579 298
BsaI GGTCTCn'nnnn_
Cuts at: 159 1210 3963 159
Size: 1051 2753 455
Fragments arranged by size:
2753 1051 455
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1448 1969 2647 3058 3205 3479 1076
Size: 372 521 678 411 147 274 1856
Fragments arranged by size:
1856 678 521 411 372 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 1165
Size: 30 4229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 502
Size: 4259
BseYI C'CCAG_C
Cuts at: 608 911 1684 2442 3107 3515 608
Size: 303 773 758 665 408 1352
Fragments arranged by size:
1352 773 758 665 408 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 708
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3077 104
Size: 1697 46 1230 1286
Fragments arranged by size:
1697 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1013 1273 2051 2241 3101 1013
Size: 260 778 190 860 2171
Fragments arranged by size:
2171 860 778 260 190
Bsp1286I G_dGCh'C
Cuts at: 116 918 1013 1102 1273 1884 1977 2051 2241
Size: 802 95 89 171 611 93 74 190
Cuts at: 2241 2303 3101 116
Size: 62 798 1274
Fragments arranged by size:
1274 802 798 611 190 171 95 93
89 74 62
BspEI T'CCGG_A
Cuts at: 1076 1076
Size: 4259
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 1309 1523 1825 2206 419
Size: 890 214 302 381 2472
Fragments arranged by size:
2472 890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 696 921 985 1106 2551 2605 4089 4254
Size: 646 225 64 121 1445 54 1484 165
Cuts at: 4254 50
Size: 55
Fragments arranged by size:
1484 1445 646 225 165 121 64 55
54
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 1144 2257 2438 1144
Size: 1113 181 2965
Fragments arranged by size:
2965 1113 181
BssSI C'ACGA_G
Cuts at: 2530 3238 2530
Size: 708 3551
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1284 1751 1774 2109 2355 2661 2673 2759
Size: 1283 467 23 335 246 306 12 86
Cuts at: 2759 3811 1
Size: 1052 449
Fragments arranged by size:
1283 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 728 107
Size: 621 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 504 888 1125 2209 2390 2553 504
Size: 384 237 1084 181 163 2210
Fragments arranged by size:
2210 1084 384 237 181 163
BtsI GCAGTG_nn'
Cuts at: 1502 1502
Size: 4259
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1224 1965 3309 1224
Size: 741 1344 2174
Fragments arranged by size:
2174 1344 741
EaeI y'GGCC_r
Cuts at: 1844 2018 2409 2436 3781 1844
Size: 174 391 27 1345 2322
Fragments arranged by size:
2322 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 592 724 928 982 1251 2282 2492 592
Size: 132 204 54 269 1031 210 2359
Fragments arranged by size:
2359 1031 269 210 204 132 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 571 2467 3209 3355 4069 571
Size: 1896 742 146 714 761
Fragments arranged by size:
1896 761 742 714 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 963 2084 2516 2869 3531 310
Size: 653 1121 432 353 662 1038
Fragments arranged by size:
1121 1038 662 653 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 789 789
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 112 1398 112
Size: 1286 2973
EcoRI G'AATT_C
Cuts at: 1153 1263 1153
Size: 110 4149
EcoRV GAT'ATC
Cuts at: 177 177
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 534 605 868 1693 1941 3171 3942 534
Size: 71 263 825 248 1230 771 851
Fragments arranged by size:
1230 851 825 771 263 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1124 1156 1165 1197 2101 2131 2133 2163 1124
Size: 32 9 32 904 30 2 30 3220
Fragments arranged by size:
3220 904 32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 168 1242 1298 168
Size: 1074 56 3129
Fragments arranged by size:
3129 1074 56
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3018 3202 3018
Size: 184 4075
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 2248 2375 2248
Size: 127 4132
MspA1I CmG'CkG
Cuts at: 129 677 1370 1684 2044 2828 3073 129
Size: 548 693 314 360 784 245 1315
Fragments arranged by size:
1315 784 693 548 360 314 245
NaeI GCC'GGC
Cuts at: 1146 2440 1146
Size: 1294 2965
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 107 107
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 1144 2438 1144
Size: 1294 2965
NspI r_CATG'y
Cuts at: 1312 1812 3415 1312
Size: 500 1603 2156
Fragments arranged by size:
2156 1603 500
PciI A'CATG_T
Cuts at: 3411 3411
Size: 4259
PfoI T'CCnGG_A
Cuts at: 287 287
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 4227
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 4259
PstI C_TGCA'G
Cuts at: 775 1306 1991 775
Size: 531 685 3043
Fragments arranged by size:
3043 685 531
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 677 1684 2044 677
Size: 1007 360 2892
Fragments arranged by size:
2892 1007 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 928 2282 2492 928
Size: 1354 210 2695
Fragments arranged by size:
2695 1354 210
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 991 3776 991
Size: 2785 1474
SfcI C'TryA_G
Cuts at: 33 771 1302 1987 2955 3146 33
Size: 738 531 685 968 191 1146
Fragments arranged by size:
1146 968 738 685 531 191
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 871 1414 2329 3043 3305 871
Size: 543 915 714 262 1825
Fragments arranged by size:
1825 915 714 543 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StyI C'CwwG_G
Cuts at: 107 785 833 1393 1747 107
Size: 678 48 560 354 2619
Fragments arranged by size:
2619 678 560 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 619 1842 1975 2434 2643 141
Size: 478 1223 133 459 209 1757
Fragments arranged by size:
1757 1223 478 459 209 133
TatI w'GTAC_w
Cuts at: 347 767 989 3774 347
Size: 420 222 2785 832
Fragments arranged by size:
2785 832 420 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 163 2056 163
Size: 1893 2366
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 126 1754 126
Size: 1628 2631
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI