Restriction map for
pF1KB5523
(Circular) (Six-base) MAPSORT of: pF1KB5523.seq Check: 1507 from: 1 to: 10529
pF1KB5523 10529 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1530 3300 1530
Size: 1770 8759
AatII G_ACGT'C
Cuts at: 4713 4713
Size: 10529
AccI GT'mk_AC
Cuts at: 7567 7567
Size: 10529
Acc65I G'GTAC_C
Cuts at: 7545 7545
Size: 10529
AclI AA'CG_TT
Cuts at: 7523 10369 7523
Size: 2846 7683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 287 337 398 650 847 1316 2914 3547
Size: 4 50 61 252 197 469 1598 633
Cuts at: 3547 3590 3605 5269 5692 5854 6028 8354 8786
Size: 43 15 1664 423 162 174 2326 432
Cuts at: 8786 9139 9801 283
Size: 353 662 1011
Fragments arranged by size:
2326 1664 1598 1011 662 633 469 432
423 353 252 197 174 162 61 50
43 15 4
AfeI AGC'GCT
Cuts at: 883 1619 3748 4135 883
Size: 736 2129 387 7277
Fragments arranged by size:
7277 2129 736 387
AflII C'TTAA_G
Cuts at: 1624 1624
Size: 10529
AflIII A'CryG_T
Cuts at: 6249 7881 9681 9962 6249
Size: 1632 1800 281 6816
Fragments arranged by size:
6816 1800 1632 281
AhdI GACnn_n'nnGTC
Cuts at: 3579 4161 5436 3579
Size: 582 1275 8672
Fragments arranged by size:
8672 1275 582
AleI CACnn'nnGTG
Cuts at: 1328 1328
Size: 10529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1528 2810 2988 3253 4441 4643 7033 1528
Size: 1282 178 265 1188 202 2390 5024
Fragments arranged by size:
5024 2390 1282 1188 265 202 178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7303 7335 7303
Size: 32 10497
AlwNI CAG_nnn'CTG
Cuts at: 225 648 825 984 1386 2445 2571 2763 3768
Size: 423 177 159 402 1059 126 192 1005
Cuts at: 3768 3918 4320 5067 5511 5640 5703 6312 7404
Size: 150 402 747 444 129 63 609 1092
Cuts at: 7404 7834 9272 225
Size: 430 1438 1482
Fragments arranged by size:
1482 1438 1092 1059 1005 747 609 444
430 423 402 402 192 177 159 150
129 126 63
ApaI G_GGCC'C
Cuts at: 1809 7353 1809
Size: 5544 4985
ApaLI G'TGCA_C
Cuts at: 4047 9367 4047
Size: 5320 5209
ApoI r'AATT_y
Cuts at: 676 1690 7087 7533 9882 10138 676
Size: 1014 5397 446 2349 256 1067
Fragments arranged by size:
5397 2349 1067 1014 446 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10529
AvaI C'yCGr_G
Cuts at: 3056 3200 4018 4496 5690 7409 7549 3056
Size: 144 818 478 1194 1719 140 6036
Fragments arranged by size:
6036 1719 1194 818 478 144 140
BamHI G'GATC_C
Cuts at: 4848 4922 6767 7242 7554 4848
Size: 74 1845 475 312 7823
Fragments arranged by size:
7823 1845 475 312 74
BanI G'GyrC_C
Cuts at: 7545 7959 8207 8242 7545
Size: 414 248 35 9832
Fragments arranged by size:
9832 414 248 35
BanII G_rGCy'C
Cuts at: 1809 1862 1970 2423 2522 5473 7353 7394 7475
Size: 53 108 453 99 2951 1880 41 81
Cuts at: 7475 7543 8573 1809
Size: 68 1030 3765
Fragments arranged by size:
3765 2951 1880 1030 453 108 99 81
68 53 41
BbeI G_GCGC'C
Cuts at: 7963 8211 7963
Size: 248 10281
BbsI GAAGACnn'nnnn_
Cuts at: 1037 1355 1592 1643 2879 3578 4037 4526 4577
Size: 318 237 51 1236 699 459 489 51
Cuts at: 4577 6112 6125 1037
Size: 1535 13 5441
Fragments arranged by size:
5441 1535 1236 699 489 459 318 237
51 51 13
BbvCI CC'TCA_GC
Cuts at: 1055 2882 4229 6526 7354 1055
Size: 1827 1347 2297 828 4230
Fragments arranged by size:
4230 2297 1827 1347 828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2140 2174 2860 2894 5277 5311 5794 5828 2140
Size: 34 686 34 2383 34 483 34 6841
Fragments arranged by size:
6841 2383 686 483 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 278 8427 9483 10529 278
Size: 8149 1056 1046 278
Fragments arranged by size:
8149 1056 1046 278
BclI T'GATC_A
Cuts at: 782 2075 4556 7292 8049 782
Size: 1293 2481 2736 757 3262
Fragments arranged by size:
3262 2736 2481 1293 757
BglI GCCn_nnn'nGGC
Cuts at: 2395 4501 5005 5149 6271 6403 7348 2395
Size: 2106 504 144 1122 132 945 5576
Fragments arranged by size:
5576 2106 1122 945 504 144 132
BglII A'GATC_T
Cuts at: 1014 2232 2919 8044 10081 1014
Size: 1218 687 5125 2037 1462
Fragments arranged by size:
5125 2037 1462 1218 687
BlpI GC'TnA_GC
Cuts at: 1964 7641 1964
Size: 5677 4852
Bme1580I G_kGCm'C
Cuts at: 1662 1809 4051 7353 8154 8247 9371 1662
Size: 147 2242 3302 801 93 1124 2820
Fragments arranged by size:
3302 2820 2242 1124 801 147 93
BmgBI CAC'GTC
Cuts at: 588 2501 6066 588
Size: 1913 3565 5051
Fragments arranged by size:
5051 3565 1913
BmrI ACTGGGnnnn_n'
Cuts at: 1143 3017 3183 3352 7741 7822 8157 10301 1143
Size: 1874 166 169 4389 81 335 2144 1371
Fragments arranged by size:
4389 2144 1874 1371 335 169 166 81
BmtI G_CTAG'C
Cuts at: 1816 4210 1816
Size: 2394 8135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5964 8896 5964
Size: 2932 7597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 670 1027 1486 2239 2785 3235 3478 3598
Size: 375 357 459 753 546 450 243 120
Cuts at: 3598 4089 4261 4417 6010 6367 6652 6663 6700
Size: 491 172 156 1593 357 285 11 37
Cuts at: 6700 7051 295
Size: 351 3773
Fragments arranged by size:
3773 1593 753 546 491 459 450 375
357 357 351 285 243 172 156 120
37 11
Bpu10I CC'TnA_GC
Cuts at: 1055 2882 4141 4229 5013 6526 7354 1055
Size: 1827 1259 88 784 1513 828 4230
Fragments arranged by size:
4230 1827 1513 1259 828 784 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 2398 7705 8584 9298 9596 1846
Size: 552 5307 879 714 298 2779
Fragments arranged by size:
5307 2779 879 714 552 298
BsaI GGTCTCn'nnnn_
Cuts at: 956 6119 6631 6769 10233 956
Size: 5163 512 138 3464 1252
Fragments arranged by size:
5163 3464 1252 512 138
BsaAI yAC'GTr
Cuts at: 3168 7377 7452 7494 7882 3168
Size: 4209 75 42 388 5815
Fragments arranged by size:
5815 4209 388 75 42
BsaBI GATnn'nnATC
Cuts at: 6 236 8043 10080 6
Size: 230 7807 2037 455
Fragments arranged by size:
7807 2037 455 230
BsaHI Gr'CG_yC
Cuts at: 216 4710 6509 7960 8208 8910 10406 216
Size: 4494 1799 1451 248 702 1496 339
Fragments arranged by size:
4494 1799 1496 1451 702 339 248
BsaWI w'CCGG_w
Cuts at: 4222 4259 4919 7718 8239 8917 9328 9475 9749
Size: 37 660 2799 521 678 411 147 274
Cuts at: 9749 4222
Size: 5002
Fragments arranged by size:
5002 2799 678 660 521 411 274 147
37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 389 419 7417 7447 389
Size: 30 6998 30 3471
Fragments arranged by size:
6998 3471 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 787 1462 2509 3232 3472 3616 5449 5638 5953
Size: 675 1047 723 240 144 1833 189 315
Cuts at: 5953 6139 6454 6510 6649 6724 6781 6940 7087
Size: 186 315 56 139 75 57 159 147
Cuts at: 7087 7342 7402 787
Size: 255 60 3914
Fragments arranged by size:
3914 1833 1047 723 675 315 315 255
240 189 186 159 147 144 139 75
60 57 56
BseYI C'CCAG_C
Cuts at: 636 1232 1288 1808 2747 2783 6175 6740 6836
Size: 596 56 520 939 36 3392 565 96
Cuts at: 6836 6980 7005 7954 8712 9377 9785 636
Size: 144 25 949 758 665 408 1380
Fragments arranged by size:
3392 1380 949 939 758 665 596 565
520 408 144 96 56 36 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 472 583 2266 3628 4109 4409 5389 5524
Size: 69 111 1683 1362 481 300 980 135
Cuts at: 5524 5880 6160 403
Size: 356 280 4772
Fragments arranged by size:
4772 1683 1362 980 481 356 300 280
135 111 69
BsiEI CG_ry'CG
Cuts at: 104 3141 5487 8071 8117 9347 104
Size: 3037 2346 2584 46 1230 1286
Fragments arranged by size:
3037 2584 2346 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1325 1862 1970 3494 4051 5660 6962 7394 7475
Size: 537 108 1524 557 1609 1302 432 81
Cuts at: 7475 7543 8321 8511 9371 1325
Size: 68 778 190 860 2483
Fragments arranged by size:
2483 1609 1524 1302 860 778 557 537
432 190 108 81 68
BsiWI C'GTAC_G
Cuts at: 3164 3164
Size: 10529
BsmI GAATG_Cn'
Cuts at: 2655 2655
Size: 10529
BsmBI CGTCTCn'nnnn_
Cuts at: 2491 7437 2491
Size: 4946 5583
Bsp1286I G_dGCh'C
Cuts at: 1325 1662 1809 1862 1970 2423 2522 3494 4051
Size: 337 147 53 108 453 99 972 557
Cuts at: 4051 5473 5660 6962 7353 7394 7475 7543 8154
Size: 1422 187 1302 391 41 81 68 611
Cuts at: 8154 8247 8321 8511 8573 9371 1325
Size: 93 74 190 62 798 2483
Fragments arranged by size:
2483 1422 1302 972 798 611 557 453
391 337 190 187 147 108 99 93
81 74 68 62 53 41
BspHI T'CATG_A
Cuts at: 708 6290 7289 8961 708
Size: 5582 999 1672 2276
Fragments arranged by size:
5582 2276 1672 999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 1520 1530 3300 3471 4043 4136 5201 7190
Size: 1361 10 1770 171 572 93 1065 1989
Cuts at: 7190 7579 7793 8095 8476 159
Size: 389 214 302 381 2212
Fragments arranged by size:
2212 1989 1770 1361 1065 572 389 381
302 214 171 93 10
BsrBI CCG'CTC
Cuts at: 50 1456 3445 6518 7508 8821 8875 10359 10524
Size: 1406 1989 3073 990 1313 54 1484 165
Cuts at: 10524 50
Size: 55
Fragments arranged by size:
3073 1989 1484 1406 1313 990 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1733 1779 2169 2522 3427 4234 4692 6203 8441
Size: 46 390 353 905 807 458 1511 2238
Cuts at: 8441 1733
Size: 3821
Fragments arranged by size:
3821 2238 1511 905 807 458 390 353
46
BsrFI r'CCGG_y
Cuts at: 2975 3156 3283 4648 4679 4975 5559 8527 8708
Size: 181 127 1365 31 296 584 2968 181
Cuts at: 8708 2975
Size: 4796
Fragments arranged by size:
4796 2968 1365 584 296 181 181 127
31
BssSI C'ACGA_G
Cuts at: 997 1783 2392 2704 8800 9508 997
Size: 786 609 312 6096 708 2018
Fragments arranged by size:
6096 2018 786 708 609 312
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 2605 3615 5211 7906 1390
Size: 1215 1010 1596 2695 4013
Fragments arranged by size:
4013 2695 1596 1215 1010
BstBI TT'CG_AA
Cuts at: 8890 8890
Size: 10529
BstEII G'GTnAC_C
Cuts at: 3063 3063
Size: 10529
BstXI CCAn_nnnn'nTGG
Cuts at: 1284 2347 1284
Size: 1063 9466
BstYI r'GATC_y
Cuts at: 1 645 1014 1142 1914 2232 2919 4848 4922
Size: 644 369 128 772 318 687 1929 74
Cuts at: 4922 5627 6767 7040 7242 7554 8021 8044 8379
Size: 705 1140 273 202 312 467 23 335
Cuts at: 8379 8625 8931 8943 9029 10081 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1929 1140 1052 772 705 687 644 467
449 369 335 318 312 306 273 246
202 128 86 74 23 12
Bsu36I CC'TnA_GG
Cuts at: 211 770 5512 211
Size: 559 4742 5228
Fragments arranged by size:
5228 4742 559
BtgI C'CryG_G
Cuts at: 107 2423 2654 2993 3679 3710 4926 6620 6920
Size: 2316 231 339 686 31 1216 1694 300
Cuts at: 6920 6968 7187 107
Size: 48 219 3449
Fragments arranged by size:
3449 2316 1694 1216 686 339 300 231
219 48 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4626 5383 8479 8660 8823 4626
Size: 757 3096 181 163 6332
Fragments arranged by size:
6332 3096 757 181 163
BtsI GCAGTG_nn'
Cuts at: 1384 1507 3235 3294 4986 5295 6215 6991 7772
Size: 123 1728 59 1692 309 920 776 781
Cuts at: 7772 1384
Size: 4141
Fragments arranged by size:
4141 1728 1692 920 781 776 309 123
59
ClaI AT'CG_AT
Cuts at: 240 4271 240
Size: 4031 6498
DraI TTT'AAA
Cuts at: 7528 7528
Size: 10529
DraIII CAC_nnn'GTG
Cuts at: 3711 3711
Size: 10529
DrdI GACnn_nn'nnGTC
Cuts at: 5866 7429 8235 9579 5866
Size: 1563 806 1344 6816
Fragments arranged by size:
6816 1563 1344 806
EaeI y'GGCC_r
Cuts at: 957 1739 2010 2349 2447 3713 3770 4650 5484
Size: 782 271 339 98 1266 57 880 834
Cuts at: 5484 5786 6120 6314 6557 7257 8114 8288 8679
Size: 302 334 194 243 700 857 174 391
Cuts at: 8679 8706 10051 957
Size: 27 1345 1435
Fragments arranged by size:
1435 1345 1266 880 857 834 782 700
391 339 334 302 271 243 194 174
98 57 27
EagI C'GGCC_G
Cuts at: 5484 8114 5484
Size: 2630 7899
EarI CTCTTCn'nnn_
Cuts at: 263 626 677 1525 2479 3043 3367 3851 4595
Size: 363 51 848 954 564 324 484 744
Cuts at: 4595 5702 5741 5955 6289 6388 6809 8552 8762
Size: 1107 39 214 334 99 421 1743 210
Cuts at: 8762 263
Size: 2030
Fragments arranged by size:
2030 1743 1107 954 848 744 564 484
421 363 334 324 214 210 99 51
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6076 8737 9479 9625 10339 6076
Size: 2661 742 146 714 6266
Fragments arranged by size:
6266 2661 742 714 146
EcoICRI GAG'CTC
Cuts at: 1860 1968 7392 7473 7541 1860
Size: 108 5424 81 68 4848
Fragments arranged by size:
5424 4848 108 81 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 287 295 337 398 650 670 847 1027
Size: 4 8 42 61 252 20 177 180
Cuts at: 1027 1316 1486 2239 2785 2914 3235 3478 3547
Size: 289 170 753 546 129 321 243 69
Cuts at: 3547 3590 3598 3605 4089 4261 4417 5269 5692
Size: 43 8 7 484 172 156 852 423
Cuts at: 5692 5854 6010 6028 6367 6652 6663 6700 7051
Size: 162 156 18 339 285 11 37 351
Cuts at: 7051 8354 8786 9139 9801 283
Size: 1303 432 353 662 1011
Fragments arranged by size:
1303 1011 852 753 662 546 484 432
423 353 351 339 321 289 285 252
243 180 177 172 170 162 156 156
129 69 61 43 42 37 20 18
11 8 8 7 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1444 2275 4399 1444
Size: 831 2124 7574
Fragments arranged by size:
7574 2124 831
EcoO109I rG'GnC_Cy
Cuts at: 171 831 2760 3522 3915 3948 4155 5142 5253
Size: 660 1929 762 393 33 207 987 111
Cuts at: 5253 5490 5964 6378 6627 6800 6824 6906 6930
Size: 237 474 414 249 173 24 82 24
Cuts at: 6930 7077 7350 7668 171
Size: 147 273 318 3032
Fragments arranged by size:
3032 1929 987 762 660 474 414 393
318 273 249 237 207 173 147 111
82 33 24 24
EcoRI G'AATT_C
Cuts at: 7087 7533 7087
Size: 446 10083
EcoRV GAT'ATC
Cuts at: 3084 3084
Size: 10529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 6250 8548 1639
Size: 4611 2298 3620
Fragments arranged by size:
4611 3620 2298
FspI TGC'GCA
Cuts at: 1483 5552 7015 8310 1483
Size: 4069 1463 1295 3702
Fragments arranged by size:
4069 3702 1463 1295
FspAI rTGC'GCAy
Cuts at: 7015 7015
Size: 10529
HaeII r_GCGC'y
Cuts at: 885 1462 1621 3750 3803 4002 4137 5343 7343
Size: 577 159 2129 53 199 135 1206 2000
Cuts at: 7343 7963 8211 9441 10212 885
Size: 620 248 1230 771 1202
Fragments arranged by size:
2129 2000 1230 1206 1202 771 620 577
248 199 159 135 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2800 2832 2860 2892 2904 2936 3243 3275 3565
Size: 32 28 32 12 32 307 32 290
Cuts at: 3565 3597 3884 3916 4147 4179 4416 4448 4599
Size: 32 287 32 231 32 237 32 151
Cuts at: 4599 4631 4723 4755 5422 5454 5897 5929 6016
Size: 32 92 32 667 32 443 32 87
Cuts at: 6016 6048 7138 7170 8371 8401 8403 8433 2800
Size: 32 1090 32 1201 30 2 30 4896
Fragments arranged by size:
4896 1201 1090 667 443 307 290 287
237 231 151 92 87 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 28 12 2
HincII GTy'rAC
Cuts at: 2031 7568 2031
Size: 5537 4992
HindIII A'AGCT_T
Cuts at: 349 1904 2125 2698 2741 2906 5846 6340 6488
Size: 1555 221 573 43 165 2940 494 148
Cuts at: 6488 349
Size: 4390
Fragments arranged by size:
4390 2940 1555 573 494 221 165 148
43
KasI G'GCGC_C
Cuts at: 7959 8207 7959
Size: 248 10281
KpnI G_GTAC'C
Cuts at: 7549 7549
Size: 10529
MfeI C'AATT_G
Cuts at: 617 617
Size: 10529
MluI A'CGCG_T
Cuts at: 9962 9962
Size: 10529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 895 1946 3347 3444 4791 6623 9288 9472 895
Size: 1051 1401 97 1347 1832 2665 184 1952
Fragments arranged by size:
2665 1952 1832 1401 1347 1051 184 97
MscI TGG'CCA
Cuts at: 1741 2012 2351 3715 5788 6316 7259 8290 1741
Size: 271 339 1364 2073 528 943 1031 3980
Fragments arranged by size:
3980 2073 1364 1031 943 528 339 271
MslI CAynn'nnrTG
Cuts at: 1328 2357 3827 7015 8518 8645 1328
Size: 1029 1470 3188 1503 127 3212
Fragments arranged by size:
3212 3188 1503 1470 1029 127
MspA1I CmG'CkG
Cuts at: 822 1232 3768 4320 4452 5050 5637 5679 5686
Size: 410 2536 552 132 598 587 42 7
Cuts at: 5686 5700 6422 6555 6699 6996 7005 7640 7954
Size: 14 722 133 144 297 9 635 314
Cuts at: 7954 8314 9098 9343 822
Size: 360 784 245 2008
Fragments arranged by size:
2536 2008 784 722 635 598 587 552
410 360 314 297 245 144 133 132
42 14 9 7
NaeI GCC'GGC
Cuts at: 2977 3158 3285 4650 4681 4977 5561 8710 2977
Size: 181 127 1365 31 296 584 3149 4796
Fragments arranged by size:
4796 3149 1365 584 296 181 127 31
NarI GG'CG_CC
Cuts at: 7960 8208 7960
Size: 248 10281
NcoI C'CATG_G
Cuts at: 107 2423 2654 6620 6920 7187 107
Size: 2316 231 3966 300 267 3449
Fragments arranged by size:
3966 3449 2316 300 267 231
NgoMIV G'CCGG_C
Cuts at: 2975 3156 3283 4648 4679 4975 5559 8708 2975
Size: 181 127 1365 31 296 584 3149 4796
Fragments arranged by size:
4796 3149 1365 584 296 181 127 31
NheI G'CTAG_C
Cuts at: 1812 4206 1812
Size: 2394 8135
NspI r_CATG'y
Cuts at: 658 1171 2269 3366 4909 7014 7020 7582 8082
Size: 513 1098 1097 1543 2105 6 562 500
Cuts at: 8082 9685 658
Size: 1603 1502
Fragments arranged by size:
2105 1603 1543 1502 1098 1097 562 513
500 6
PasI CC'CwG_GG
Cuts at: 3647 7170 3647
Size: 3523 7006
PciI A'CATG_T
Cuts at: 9681 9681
Size: 10529
PflMI CCAn_nnn'nTGG
Cuts at: 558 648 3792 3848 6312 558
Size: 90 3144 56 2464 4775
Fragments arranged by size:
4775 3144 2464 90 56
PfoI T'CCnGG_A
Cuts at: 152 2477 2818 3212 4151 4595 5417 5440 5576
Size: 2325 341 394 939 444 822 23 136
Cuts at: 5576 6076 6938 7229 152
Size: 500 862 291 3452
Fragments arranged by size:
3452 2325 939 862 822 500 444 394
341 291 136 23
PmeI GTTT'AAAC
Cuts at: 7528 7528
Size: 10529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8975 9007 8975
Size: 32 10497
PpuMI rG'GwC_Cy
Cuts at: 171 2760 3522 3948 4155 5253 5490 6378 6800
Size: 2589 762 426 207 1098 237 888 422
Cuts at: 6800 6824 6906 171
Size: 24 82 3794
Fragments arranged by size:
3794 2589 1098 888 762 426 422 237
207 82 24
PsiI TTA'TAA
Cuts at: 323 1910 323
Size: 1587 8942
PspOMI G'GGCC_C
Cuts at: 1805 7349 1805
Size: 5544 4985
PstI C_TGCA'G
Cuts at: 548 845 1368 1517 1835 2682 2780 4040 4133
Size: 297 523 149 318 847 98 1260 93
Cuts at: 4133 4451 4622 6003 6185 6689 7576 8261 548
Size: 318 171 1381 182 504 887 685 2816
Fragments arranged by size:
2816 1381 1260 887 847 685 523 504
318 318 297 182 171 149 98 93
PvuI CG_AT'CG
Cuts at: 104 3141 104
Size: 3037 7492
PvuII CAG'CTG
Cuts at: 1232 3768 4320 4452 5679 5686 6422 6555 6996
Size: 2536 552 132 1227 7 736 133 441
Cuts at: 6996 7005 7954 8314 1232
Size: 9 949 360 3447
Fragments arranged by size:
3447 2536 1227 949 736 552 441 360
133 132 9 7
RsrII CG'GwC_CG
Cuts at: 8724 8724
Size: 10529
SacI G_AGCT'C
Cuts at: 1862 1970 7394 7475 7543 1862
Size: 108 5424 81 68 4848
Fragments arranged by size:
5424 4848 108 81 68
SalI G'TCGA_C
Cuts at: 7566 7566
Size: 10529
SanDI GG'GwC_CC
Cuts at: 6824 6824
Size: 10529
SapI GCTCTTCn'nnn_
Cuts at: 263 626 1525 2479 8552 8762 263
Size: 363 899 954 6073 210 2030
Fragments arranged by size:
6073 2030 954 899 363 210
SbfI CC_TGCA'GG
Cuts at: 4040 7576 4040
Size: 3536 6993
ScaI AGT'ACT
Cuts at: 568 2791 10046 568
Size: 2223 7255 1051
Fragments arranged by size:
7255 2223 1051
SexAI A'CCwGG_T
Cuts at: 6610 6610
Size: 10529
SfcI C'TryA_G
Cuts at: 33 544 841 1364 1513 1831 2678 2776 3415
Size: 511 297 523 149 318 847 98 639
Cuts at: 3415 3760 4036 4129 4447 4618 5999 6181 6652
Size: 345 276 93 318 171 1381 182 471
Cuts at: 6652 6685 7572 8257 9225 9416 33
Size: 33 887 685 968 191 1146
Fragments arranged by size:
1381 1146 968 887 847 685 639 523
511 471 345 318 318 297 276 191
182 171 149 98 93 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 6271 6271
Size: 10529
SfoI GGC'GCC
Cuts at: 7961 8209 7961
Size: 248 10281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10529
SmaI CCC'GGG
Cuts at: 3202 4498 5692 7411 7551 3202
Size: 1296 1194 1719 140 6180
Fragments arranged by size:
6180 1719 1296 1194 140
SmlI C'TyrA_G
Cuts at: 1624 1861 2413 7684 8599 9313 9575 1624
Size: 237 552 5271 915 714 262 2578
Fragments arranged by size:
5271 2578 915 714 552 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10529
SphI G_CATG'C
Cuts at: 4909 7014 7020 7582 8082 4909
Size: 2105 6 562 500 7356
Fragments arranged by size:
7356 2105 562 500 6
StuI AGG'CCT
Cuts at: 4066 4405 4504 4624 6187 4066
Size: 339 99 120 1563 8408
Fragments arranged by size:
8408 1563 339 120 99
StyI C'CwwG_G
Cuts at: 107 1151 1601 1997 2042 2423 2654 3981 4211
Size: 1044 450 396 45 381 231 1327 230
Cuts at: 4211 4661 5714 6260 6473 6620 6920 7187 7382
Size: 450 1053 546 213 147 300 267 195
Cuts at: 7382 7663 8017 107
Size: 281 354 2619
Fragments arranged by size:
2619 1327 1053 1044 546 450 450 396
381 354 300 281 267 231 230 213
195 147 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 194 1224 2318 3334 4102 8112 8245 8704 8913
Size: 1030 1094 1016 768 4010 133 459 209
Cuts at: 8913 194
Size: 1810
Fragments arranged by size:
4010 1810 1094 1030 1016 768 459 209
133
TatI w'GTAC_w
Cuts at: 566 1938 2789 3810 6950 10044 566
Size: 1372 851 1021 3140 3094 1051
Fragments arranged by size:
3140 3094 1372 1051 1021 851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6241 6273 7227 7259 10046 10078 6241
Size: 32 954 32 2787 32 6692
Fragments arranged by size:
6692 2787 954 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1036 8326 1036
Size: 7290 3239
XbaI T'CTAG_A
Cuts at: 1026 7560 1026
Size: 6534 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 408 2049 6627 8024 408
Size: 1641 4578 1397 2913
Fragments arranged by size:
4578 2913 1641 1397
XmaI C'CCGG_G
Cuts at: 3200 4496 5690 7409 7549 3200
Size: 1296 1194 1719 140 6180
Fragments arranged by size:
6180 1719 1296 1194 140
XmnI GAAnn'nnTTC
Cuts at: 296 686 1619 296
Size: 390 933 9206
Fragments arranged by size:
9206 933 390
ZraI GAC'GTC
Cuts at: 4711 4711
Size: 10529
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AscI AvrII BaeI BfrBI BspEI BsrGI BssHII
BstZ17I CspCI FseI HpaI NdeI NotI NruI NsiI
PacI PmlI PshAI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI SwaI XhoI