Restriction map for
pF1KB5528
(Circular) (Six-base) MAPSORT of: pF1KB5528.seq Check: 4084 from: 1 to: 7382
pF1KB5528 7382 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1600 1600
Size: 7382
AccI GT'mk_AC
Cuts at: 4420 4420
Size: 7382
Acc65I G'GTAC_C
Cuts at: 4398 4398
Size: 7382
AclI AA'CG_TT
Cuts at: 7222 7222
Size: 7382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1198 3475 3613 3927 5207 5639 6386 6654
Size: 753 2277 138 314 1280 432 747 268
Cuts at: 6654 445
Size: 1173
Fragments arranged by size:
2277 1280 1173 753 747 432 314 268
138
AflII C'TTAA_G
Cuts at: 616 616
Size: 7382
AflIII A'CryG_T
Cuts at: 4734 5838 6815 4734
Size: 1104 977 5301
Fragments arranged by size:
5301 1104 977
AgeI A'CCGG_T
Cuts at: 5770 5770
Size: 7382
AleI CACnn'nnGTG
Cuts at: 1886 1886
Size: 7382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 803 3817 4262 803
Size: 3014 445 3923
Fragments arranged by size:
3923 3014 445
AlwNI CAG_nnn'CTG
Cuts at: 3639 4687 6254 3639
Size: 1048 1567 4767
Fragments arranged by size:
4767 1567 1048
ApaLI G'TGCA_C
Cuts at: 1615 2412 4282 6152 1615
Size: 797 1870 1870 2845
Fragments arranged by size:
2845 1870 1870 797
ApoI r'AATT_y
Cuts at: 621 954 2184 2907 3201 4209 4386 6735 6991
Size: 333 1230 723 294 1008 177 2349 256
Cuts at: 6991 621
Size: 1012
Fragments arranged by size:
2349 1230 1012 1008 723 333 294 256
177
AseI AT'TA_AT
Cuts at: 20 20
Size: 7382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7382
AvaI C'yCGr_G
Cuts at: 4402 4402
Size: 7382
BamHI G'GATC_C
Cuts at: 132 4407 132
Size: 4275 3107
BanI G'GyrC_C
Cuts at: 4398 4812 5060 5095 4398
Size: 414 248 35 6685
Fragments arranged by size:
6685 414 248 35
BanII G_rGCy'C
Cuts at: 1490 3377 3754 4396 5426 1490
Size: 1887 377 642 1030 3446
Fragments arranged by size:
3446 1887 1030 642 377
BbeI G_GCGC'C
Cuts at: 4816 5064 4816
Size: 248 7134
BbsI GAAGACnn'nnnn_
Cuts at: 605 740 2931 605
Size: 135 2191 5056
Fragments arranged by size:
5056 2191 135
BbvCI CC'TCA_GC
Cuts at: 2195 2195
Size: 7382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2632 2666 4094 4128 2632
Size: 34 1428 34 5886
Fragments arranged by size:
5886 1428 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2118 5280 6041 7382 2118
Size: 3162 761 1341 2118
Fragments arranged by size:
3162 2118 1341 761
BclI T'GATC_A
Cuts at: 4362 4902 4362
Size: 540 6842
BfrBI ATG'CAT
Cuts at: 2127 2127
Size: 7382
BglII A'GATC_T
Cuts at: 4897 6934 4897
Size: 2037 5345
BlpI GC'TnA_GC
Cuts at: 254 4494 254
Size: 4240 3142
Bme1580I G_kGCm'C
Cuts at: 1619 1683 2416 4286 5007 5100 6156 1619
Size: 64 733 1870 721 93 1056 2845
Fragments arranged by size:
2845 1870 1056 733 721 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 1093 1655 2234 2874 2977 3136 4594 4675 5010
Size: 562 579 640 103 159 1458 81 335
Cuts at: 5010 7154 1093
Size: 2144 1321
Fragments arranged by size:
2144 1458 1321 640 579 562 335 159
103 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1900 5749 1900
Size: 3849 3533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 2099 2120 2510 2696 164
Size: 1935 21 390 186 4850
Fragments arranged by size:
4850 1935 390 186 21
Bpu10I CC'TnA_GC
Cuts at: 2195 2195
Size: 7382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2421 2688 2790 4558 5437 5929 6227 2421
Size: 267 102 1768 879 492 298 3576
Fragments arranged by size:
3576 1768 879 492 298 267 102
BsaI GGTCTCn'nnnn_
Cuts at: 658 3015 3582 7086 658
Size: 2357 567 3504 954
Fragments arranged by size:
3504 2357 954 567
BsaAI yAC'GTr
Cuts at: 4735 4735
Size: 7382
BsaBI GATnn'nnATC
Cuts at: 6 4896 6933 6
Size: 4890 2037 455
Fragments arranged by size:
4890 2037 455
BsaHI Gr'CG_yC
Cuts at: 4813 5061 5763 7259 4813
Size: 248 702 1496 4936
Fragments arranged by size:
4936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 4571 5092 5770 6044 6191 6602 2153
Size: 2418 521 678 274 147 411 2933
Fragments arranged by size:
2933 2418 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1506 1536 2832 2862 1506
Size: 30 1296 30 6026
Fragments arranged by size:
6026 1296 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1547 2076 2079 2117 2948 4007 1547
Size: 529 3 38 831 1059 4922
Fragments arranged by size:
4922 1059 831 529 38 3
BseYI C'CCAG_C
Cuts at: 3453 3973 4235 4807 5565 6142 6638 3453
Size: 520 262 572 758 577 496 4197
Fragments arranged by size:
4197 758 577 572 520 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 2662 2948 3262 1124
Size: 1538 286 314 5244
Fragments arranged by size:
5244 1538 314 286
BsiEI CG_ry'CG
Cuts at: 104 4924 4970 6178 104
Size: 4820 46 1208 1308
Fragments arranged by size:
4820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1619 2416 3754 4286 4396 5174 5364 6156 1619
Size: 797 1338 532 110 778 190 792 2845
Fragments arranged by size:
2845 1338 797 792 778 532 190 110
BsmI GAATG_Cn'
Cuts at: 3338 3569 3626 3338
Size: 231 57 7094
Fragments arranged by size:
7094 231 57
BsmBI CGTCTCn'nnnn_
Cuts at: 210 491 210
Size: 281 7101
Bsp1286I G_dGCh'C
Cuts at: 1490 1619 1683 2416 3377 3754 4286 4396 5007
Size: 129 64 733 961 377 532 110 611
Cuts at: 5007 5100 5174 5364 5426 6156 1490
Size: 93 74 190 62 730 2716
Fragments arranged by size:
2716 961 733 730 611 532 377 190
129 110 93 74 64 62
BspEI T'CCGG_A
Cuts at: 6602 6602
Size: 7382
BspHI T'CATG_A
Cuts at: 1547 3584 6558 1547
Size: 2037 2974 2371
Fragments arranged by size:
2974 2371 2037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 548 1600 2336 2970 4432 4646 4948 5329
Size: 343 1052 736 634 1462 214 302 381
Cuts at: 5329 205
Size: 2258
Fragments arranged by size:
2258 1462 1052 736 634 381 343 302
214
BsrBI CCG'CTC
Cuts at: 50 5674 5728 7212 7377 50
Size: 5624 54 1484 165 55
Fragments arranged by size:
5624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2986 4112 5294 2986
Size: 1126 1182 5074
Fragments arranged by size:
5074 1182 1126
BsrFI r'CCGG_y
Cuts at: 2887 5380 5561 5770 2887
Size: 2493 181 209 4499
Fragments arranged by size:
4499 2493 209 181
BssSI C'ACGA_G
Cuts at: 803 3572 5653 6011 803
Size: 2769 2081 358 2174
Fragments arranged by size:
2769 2174 2081 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2169 3048 3639 4759 2169
Size: 879 591 1120 4792
Fragments arranged by size:
4792 1120 879 591
BstBI TT'CG_AA
Cuts at: 5743 5743
Size: 7382
BstXI CCAn_nnnn'nTGG
Cuts at: 3084 3312 3084
Size: 228 7154
BstYI r'GATC_y
Cuts at: 1 132 964 2485 4068 4407 4874 4897 5232
Size: 131 832 1521 1583 339 467 23 335
Cuts at: 5232 5478 6490 6576 6588 6934 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1583 1521 1012 832 467 449 346 339
335 246 131 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 444 2856 444
Size: 2412 4970
BtgI C'CryG_G
Cuts at: 1625 2918 3077 3155 1625
Size: 1293 159 78 5852
Fragments arranged by size:
5852 1293 159 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5332 5513 5676 5332
Size: 181 163 7038
Fragments arranged by size:
7038 181 163
BtsI GCAGTG_nn'
Cuts at: 207 787 1917 2678 4274 4305 4625 207
Size: 580 1130 761 1596 31 320 2964
Fragments arranged by size:
2964 1596 1130 761 580 320 31
ClaI AT'CG_AT
Cuts at: 1107 1107
Size: 7382
DraI TTT'AAA
Cuts at: 323 4381 323
Size: 4058 3324
DraIII CAC_nnn'GTG
Cuts at: 2971 2971
Size: 7382
DrdI GACnn_nn'nnGTC
Cuts at: 344 5088 5946 344
Size: 4744 858 1780
Fragments arranged by size:
4744 1780 858
EaeI y'GGCC_r
Cuts at: 140 1083 3102 4198 4214 4967 5141 5532 5559
Size: 943 2019 1096 16 753 174 391 27
Cuts at: 5559 6904 140
Size: 1345 618
Fragments arranged by size:
2019 1345 1096 943 753 618 391 174
27 16
EagI C'GGCC_G
Cuts at: 4967 4967
Size: 7382
EarI CTCTTCn'nnn_
Cuts at: 1165 2474 2999 3197 3290 5405 5615 1165
Size: 1309 525 198 93 2115 210 2932
Fragments arranged by size:
2932 2115 1309 525 210 198 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5590 5900 6046 7192 5590
Size: 310 146 1146 5780
Fragments arranged by size:
5780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3752 4394 3752
Size: 642 6740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 445 1198 2099 2120 2510 2696 3475 3613
Size: 281 753 901 21 390 186 779 138
Cuts at: 3613 3927 5207 5639 6386 6654 164
Size: 314 1280 432 747 268 892
Fragments arranged by size:
1280 901 892 779 753 747 432 390
314 281 268 186 138 21
EcoNI CCTnn'n_nnAGG
Cuts at: 972 1442 1607 2855 3557 972
Size: 470 165 1248 702 4797
Fragments arranged by size:
4797 1248 702 470 165
EcoO109I rG'GnC_Cy
Cuts at: 558 1352 1741 1939 3463 3997 4126 4357 4521
Size: 794 389 198 1524 534 129 231 164
Cuts at: 4521 558
Size: 3419
Fragments arranged by size:
3419 1524 794 534 389 231 198 164
129
EcoRI G'AATT_C
Cuts at: 2184 2907 3201 4386 2184
Size: 723 294 1185 5180
Fragments arranged by size:
5180 1185 723 294
EcoRV GAT'ATC
Cuts at: 263 263
Size: 7382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1093 1958 5401 1093
Size: 865 3443 3074
Fragments arranged by size:
3443 3074 865
FspI TGC'GCA
Cuts at: 5163 5163
Size: 7382
HaeII r_GCGC'y
Cuts at: 4816 5064 6086 7065 4816
Size: 248 1022 979 5133
Fragments arranged by size:
5133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 424 456 820 852 1024 1056 1506
Size: 32 145 32 364 32 172 32 450
Cuts at: 1506 1538 1542 1574 2344 2376 2772 2804 2826
Size: 32 4 32 770 32 396 32 22
Cuts at: 2826 2832 2858 2864 3566 3598 5224 5254 5256
Size: 6 26 6 702 32 1626 30 2
Cuts at: 5256 5286 247
Size: 30 2343
Fragments arranged by size:
2343 1626 770 702 450 396 364 172
145 32 32 32 32 32 32 32
32 32 30 30 26 22 6 6
4 2
HincII GTy'rAC
Cuts at: 567 4421 567
Size: 3854 3528
HindIII A'AGCT_T
Cuts at: 1070 1070
Size: 7382
HpaI GTT'AAC
Cuts at: 567 567
Size: 7382
KasI G'GCGC_C
Cuts at: 4812 5060 4812
Size: 248 7134
KpnI G_GTAC'C
Cuts at: 4402 4402
Size: 7382
MluI A'CGCG_T
Cuts at: 6815 6815
Size: 7382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1236 3257 3843 6053 6237 1236
Size: 2021 586 2210 184 2381
Fragments arranged by size:
2381 2210 2021 586 184
MscI TGG'CCA
Cuts at: 142 1085 3104 4200 4216 5143 142
Size: 943 2019 1096 16 927 2381
Fragments arranged by size:
2381 2019 1096 943 927 16
MslI CAynn'nnrTG
Cuts at: 1312 1886 3082 4289 4336 4351 5371 5498 1312
Size: 574 1196 1207 47 15 1020 127 3196
Fragments arranged by size:
3196 1207 1196 1020 574 127 47 15
MspA1I CmG'CkG
Cuts at: 2194 3276 4493 4807 5167 6180 6425 2194
Size: 1082 1217 314 360 1013 245 3151
Fragments arranged by size:
3151 1217 1082 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2889 5563 2889
Size: 2674 4708
NarI GG'CG_CC
Cuts at: 4813 5061 4813
Size: 248 7134
NcoI C'CATG_G
Cuts at: 1625 2918 3077 3155 1625
Size: 1293 159 78 5852
Fragments arranged by size:
5852 1293 159 78
NgoMIV G'CCGG_C
Cuts at: 2887 5561 2887
Size: 2674 4708
NsiI A_TGCA'T
Cuts at: 2129 2129
Size: 7382
NspI r_CATG'y
Cuts at: 1452 3150 4350 4435 4935 5842 1452
Size: 1698 1200 85 500 907 2992
Fragments arranged by size:
2992 1698 1200 907 500 85
PasI CC'CwG_GG
Cuts at: 1438 3756 1438
Size: 2318 5064
PciI A'CATG_T
Cuts at: 5838 5838
Size: 7382
PflMI CCAn_nnn'nTGG
Cuts at: 4048 4078 4048
Size: 30 7352
PfoI T'CCnGG_A
Cuts at: 180 652 180
Size: 472 6910
PmeI GTTT'AAAC
Cuts at: 4381 4381
Size: 7382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2598 2630 6521 6553 2598
Size: 32 3891 32 3427
Fragments arranged by size:
3891 3427 32 32
PpuMI rG'GwC_Cy
Cuts at: 558 1939 3997 4126 4357 558
Size: 1381 2058 129 231 3583
Fragments arranged by size:
3583 2058 1381 231 129
PshAI GACnn'nnGTC
Cuts at: 2003 2003
Size: 7382
PsiI TTA'TAA
Cuts at: 2942 2942
Size: 7382
PstI C_TGCA'G
Cuts at: 202 1976 2177 2217 3644 4269 4429 5114 202
Size: 1774 201 40 1427 625 160 685 2470
Fragments arranged by size:
2470 1774 1427 685 625 201 160 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7382
PvuII CAG'CTG
Cuts at: 2194 3276 4807 5167 2194
Size: 1082 1531 360 4409
Fragments arranged by size:
4409 1531 1082 360
RsrII CG'GwC_CG
Cuts at: 5577 5577
Size: 7382
SacI G_AGCT'C
Cuts at: 3754 4396 3754
Size: 642 6740
SalI G'TCGA_C
Cuts at: 4419 4419
Size: 7382
SanDI GG'GwC_CC
Cuts at: 4126 4126
Size: 7382
SapI GCTCTTCn'nnn_
Cuts at: 5405 5615 5405
Size: 210 7172
SbfI CC_TGCA'GG
Cuts at: 4429 4429
Size: 7382
ScaI AGT'ACT
Cuts at: 6899 6899
Size: 7382
SexAI A'CCwGG_T
Cuts at: 560 3012 560
Size: 2452 4930
SfcI C'TryA_G
Cuts at: 33 198 413 635 869 1130 1972 2173 2213
Size: 165 215 222 234 261 842 201 40
Cuts at: 2213 2371 3640 4265 4425 5110 6103 6294 33
Size: 158 1269 625 160 685 993 191 1121
Fragments arranged by size:
1269 1121 993 842 685 625 261 234
222 215 201 191 165 160 158 40
SfoI GGC'GCC
Cuts at: 4814 5062 4814
Size: 248 7134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7382
SmaI CCC'GGG
Cuts at: 4404 4404
Size: 7382
SmlI C'TyrA_G
Cuts at: 616 2436 2703 2805 4537 5452 5944 6206 616
Size: 1820 267 102 1732 915 492 262 1792
Fragments arranged by size:
1820 1792 1732 915 492 267 262 102
SpeI A'CTAG_T
Cuts at: 67 2530 67
Size: 2463 4919
SphI G_CATG'C
Cuts at: 1452 4350 4435 4935 1452
Size: 2898 85 500 3899
Fragments arranged by size:
3899 2898 500 85
SspI AAT'ATT
Cuts at: 1773 1773
Size: 7382
StuI AGG'CCT
Cuts at: 978 3063 3189 3311 3729 3940 978
Size: 2085 126 122 418 211 4420
Fragments arranged by size:
4420 2085 418 211 126 122
StyI C'CwwG_G
Cuts at: 1326 1625 2918 3077 3155 3393 3558 4516 4870
Size: 299 1293 159 78 238 165 958 354
Cuts at: 4870 1326
Size: 3838
Fragments arranged by size:
3838 1293 958 354 299 238 165 159
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2752 2803 2959 2987 3584 4965 5098 5557 5766
Size: 51 156 28 597 1381 133 459 209
Cuts at: 5766 2752
Size: 4368
Fragments arranged by size:
4368 1381 597 459 209 156 133 51
28
TatI w'GTAC_w
Cuts at: 6897 6897
Size: 7382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 1645 1677 6899 6931 1118
Size: 32 495 32 5222 32 1569
Fragments arranged by size:
5222 1569 495 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 188 5179 188
Size: 4991 2391
XbaI T'CTAG_A
Cuts at: 2505 4413 2505
Size: 1908 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1815 2964 4877 1815
Size: 1149 1913 4320
Fragments arranged by size:
4320 1913 1149
XmaI C'CCGG_G
Cuts at: 4402 4402
Size: 7382
XmnI GAAnn'nnTTC
Cuts at: 2610 2610
Size: 7382
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
CspCI FseI FspAI MfeI NdeI NheI NotI NruI
PacI PmlI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI