Restriction map for
pF1KB5530
(Circular) (Six-base) MAPSORT of: pF1KB5530.seq Check: 9716 from: 1 to: 3473
pF1KB5530 3473 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 511 511
Size: 3473
Acc65I G'GTAC_C
Cuts at: 489 489
Size: 3473
AclI AA'CG_TT
Cuts at: 3313 3313
Size: 3473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1730 2477 2745 1298
Size: 432 747 268 2026
Fragments arranged by size:
2026 747 432 268
AflIII A'CryG_T
Cuts at: 825 1929 2906 825
Size: 1104 977 1392
Fragments arranged by size:
1392 1104 977
AgeI A'CCGG_T
Cuts at: 1861 1861
Size: 3473
AlwNI CAG_nnn'CTG
Cuts at: 778 2345 778
Size: 1567 1906
ApaLI G'TGCA_C
Cuts at: 2243 2243
Size: 3473
ApoI r'AATT_y
Cuts at: 477 2826 3082 477
Size: 2349 256 868
Fragments arranged by size:
2349 868 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3473
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3473
AvaI C'yCGr_G
Cuts at: 220 493 220
Size: 273 3200
BamHI G'GATC_C
Cuts at: 498 498
Size: 3473
BanI G'GyrC_C
Cuts at: 361 489 903 1151 1186 361
Size: 128 414 248 35 2648
Fragments arranged by size:
2648 414 248 128 35
BanII G_rGCy'C
Cuts at: 487 1517 487
Size: 1030 2443
BbeI G_GCGC'C
Cuts at: 907 1155 907
Size: 248 3225
BbsI GAAGACnn'nnnn_
Cuts at: 380 380
Size: 3473
BciVI GTATCCnnnnn_n'
Cuts at: 1371 2132 3473 1371
Size: 761 1341 1371
Fragments arranged by size:
1371 1341 761
BclI T'GATC_A
Cuts at: 993 993
Size: 3473
BglII A'GATC_T
Cuts at: 988 3025 988
Size: 2037 1436
BlpI GC'TnA_GC
Cuts at: 585 585
Size: 3473
Bme1580I G_kGCm'C
Cuts at: 1098 1191 2247 1098
Size: 93 1056 2324
Fragments arranged by size:
2324 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 685 766 1101 3245 685
Size: 81 335 2144 913
Fragments arranged by size:
2144 913 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1840 1840
Size: 3473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1528 2020 2318 649
Size: 879 492 298 1804
Fragments arranged by size:
1804 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3177 3177
Size: 3473
BsaAI yAC'GTr
Cuts at: 826 826
Size: 3473
BsaBI GATnn'nnATC
Cuts at: 6 463 987 3024 6
Size: 457 524 2037 455
Fragments arranged by size:
2037 524 457 455
BsaHI Gr'CG_yC
Cuts at: 185 904 1152 1854 3350 185
Size: 719 248 702 1496 308
Fragments arranged by size:
1496 719 702 308 248
BsaWI w'CCGG_w
Cuts at: 662 1183 1861 2135 2282 2693 662
Size: 521 678 274 147 411 1442
Fragments arranged by size:
1442 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 139 898 1656 2233 2729 139
Size: 759 758 577 496 883
Fragments arranged by size:
883 759 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1015 1061 2269 104
Size: 911 46 1208 1308
Fragments arranged by size:
1308 1208 911 46
BsiHKAI G_wGCw'C
Cuts at: 487 1265 1455 2247 487
Size: 778 190 792 1713
Fragments arranged by size:
1713 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 487 1098 1191 1265 1455 1517 2247 487
Size: 611 93 74 190 62 730 1713
Fragments arranged by size:
1713 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2693 2693
Size: 3473
BspHI T'CATG_A
Cuts at: 201 2649 201
Size: 2448 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 523 737 1039 1420 523
Size: 214 302 381 2576
Fragments arranged by size:
2576 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1765 1819 3303 3468 50
Size: 1715 54 1484 165 55
Fragments arranged by size:
1715 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1385 1385
Size: 3473
BsrFI r'CCGG_y
Cuts at: 121 1471 1652 1861 121
Size: 1350 181 209 1733
Fragments arranged by size:
1733 1350 209 181
BssSI C'ACGA_G
Cuts at: 1744 2102 1744
Size: 358 3115
BstAPI GCAn_nnn'nTGC
Cuts at: 850 850
Size: 3473
BstBI TT'CG_AA
Cuts at: 1834 1834
Size: 3473
BstYI r'GATC_y
Cuts at: 1 498 965 988 1323 1569 2581 2667 2679
Size: 497 467 23 335 246 1012 86 12
Cuts at: 2679 3025 1
Size: 346 449
Fragments arranged by size:
1012 497 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 170 305 170
Size: 135 3338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1423 1604 1767 1423
Size: 181 163 3129
Fragments arranged by size:
3129 181 163
BtsI GCAGTG_nn'
Cuts at: 716 716
Size: 3473
DraI TTT'AAA
Cuts at: 472 472
Size: 3473
DrdI GACnn_nn'nnGTC
Cuts at: 1179 2037 1179
Size: 858 2615
EaeI y'GGCC_r
Cuts at: 129 173 1058 1232 1623 1650 2995 129
Size: 44 885 174 391 27 1345 607
Fragments arranged by size:
1345 885 607 391 174 44 27
EagI C'GGCC_G
Cuts at: 1058 1058
Size: 3473
EarI CTCTTCn'nnn_
Cuts at: 238 1496 1706 238
Size: 1258 210 2005
Fragments arranged by size:
2005 1258 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1681 1991 2137 3283 1681
Size: 310 146 1146 1871
Fragments arranged by size:
1871 1146 310 146
EcoICRI GAG'CTC
Cuts at: 485 485
Size: 3473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1730 2477 2745 1298
Size: 432 747 268 2026
Fragments arranged by size:
2026 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 612 612
Size: 3473
EcoRI G'AATT_C
Cuts at: 477 477
Size: 3473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1492 1492
Size: 3473
FspI TGC'GCA
Cuts at: 1254 1254
Size: 3473
HaeII r_GCGC'y
Cuts at: 907 1155 2177 3156 907
Size: 248 1022 979 1224
Fragments arranged by size:
1224 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1315 1345 1347 1377 1315
Size: 30 2 30 3411
Fragments arranged by size:
3411 30 30 2
HincII GTy'rAC
Cuts at: 512 512
Size: 3473
KasI G'GCGC_C
Cuts at: 903 1151 903
Size: 248 3225
KpnI G_GTAC'C
Cuts at: 493 493
Size: 3473
MluI A'CGCG_T
Cuts at: 2906 2906
Size: 3473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 2144 2328 266
Size: 1878 184 1411
Fragments arranged by size:
1878 1411 184
MscI TGG'CCA
Cuts at: 175 1234 175
Size: 1059 2414
MslI CAynn'nnrTG
Cuts at: 1462 1589 1462
Size: 127 3346
MspA1I CmG'CkG
Cuts at: 128 584 898 1258 2271 2516 128
Size: 456 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 456 360 314 245
NaeI GCC'GGC
Cuts at: 123 1654 123
Size: 1531 1942
NarI GG'CG_CC
Cuts at: 904 1152 904
Size: 248 3225
NcoI C'CATG_G
Cuts at: 305 305
Size: 3473
NgoMIV G'CCGG_C
Cuts at: 121 1652 121
Size: 1531 1942
NspI r_CATG'y
Cuts at: 433 526 1026 1933 433
Size: 93 500 907 1973
Fragments arranged by size:
1973 907 500 93
PciI A'CATG_T
Cuts at: 1929 1929
Size: 3473
PmeI GTTT'AAAC
Cuts at: 472 472
Size: 3473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2612 2644 2612
Size: 32 3441
PstI C_TGCA'G
Cuts at: 520 1205 520
Size: 685 2788
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3473
PvuII CAG'CTG
Cuts at: 898 1258 898
Size: 360 3113
RsrII CG'GwC_CG
Cuts at: 1668 1668
Size: 3473
SacI G_AGCT'C
Cuts at: 487 487
Size: 3473
SalI G'TCGA_C
Cuts at: 510 510
Size: 3473
SapI GCTCTTCn'nnn_
Cuts at: 238 1496 1706 238
Size: 1258 210 2005
Fragments arranged by size:
2005 1258 210
SbfI CC_TGCA'GG
Cuts at: 520 520
Size: 3473
ScaI AGT'ACT
Cuts at: 2990 2990
Size: 3473
SfcI C'TryA_G
Cuts at: 33 516 1201 2194 2385 33
Size: 483 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 191
SfoI GGC'GCC
Cuts at: 905 1153 905
Size: 248 3225
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3473
SmaI CCC'GGG
Cuts at: 222 495 222
Size: 273 3200
SmlI C'TyrA_G
Cuts at: 628 1543 2035 2297 628
Size: 915 492 262 1804
Fragments arranged by size:
1804 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3473
SphI G_CATG'C
Cuts at: 526 1026 526
Size: 500 2973
StyI C'CwwG_G
Cuts at: 305 607 961 305
Size: 302 354 2817
Fragments arranged by size:
2817 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1056 1189 1648 1857 1056
Size: 133 459 209 2672
Fragments arranged by size:
2672 459 209 133
TatI w'GTAC_w
Cuts at: 2988 2988
Size: 3473
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2990 3022 2990
Size: 32 3441
Tth111I GACn'n_nGTC
Cuts at: 1270 1270
Size: 3473
XbaI T'CTAG_A
Cuts at: 504 504
Size: 3473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 968 168
Size: 800 2673
XmaI C'CCGG_G
Cuts at: 220 493 220
Size: 273 3200
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI