Restriction map for
pF1KB5543
(Circular) (Six-base) MAPSORT of: pF1KB5543.seq Check: 1031 from: 1 to: 4151
pF1KB5543 4151 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1976 2408 3155 3423 1976
Size: 432 747 268 2704
Fragments arranged by size:
2704 747 432 268
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 877 1456 3023 877
Size: 579 1567 2005
Fragments arranged by size:
2005 1567 579
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 790 1003 1155 3504 3760 790
Size: 213 152 2349 256 1181
Fragments arranged by size:
2349 1181 256 213 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1171 1171
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 1165 2195 1165
Size: 1030 3121
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 545 545
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 377 1671 377
Size: 1294 2857
BglII A'GATC_T
Cuts at: 417 1666 3703 417
Size: 1249 2037 865
Fragments arranged by size:
2037 1249 865
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 1776 1869 2925 1776
Size: 93 1056 3002
Fragments arranged by size:
3002 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 887 1363 1444 1779 3923 887
Size: 476 81 335 2144 1115
Fragments arranged by size:
2144 1115 476 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 275 851 857 162
Size: 113 576 6 3456
Fragments arranged by size:
3456 576 113 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 1327 2206 2698 2996 545
Size: 782 879 492 298 1700
Fragments arranged by size:
1700 879 782 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 956 956
Size: 4151
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1165 1943 2133 2925 1165
Size: 778 190 792 2391
Fragments arranged by size:
2391 792 778 190
BsmI GAATG_Cn'
Cuts at: 225 225
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 1165 1776 1869 1943 2133 2195 2925 1165
Size: 611 93 74 190 62 730 2391
Fragments arranged by size:
2391 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 717 3327 717
Size: 2610 1541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 284 2443 2497 3981 4146 50
Size: 234 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 139 2422 2780 139
Size: 2283 358 1510
Fragments arranged by size:
2283 1510 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 381 608 381
Size: 227 3924
BstYI r'GATC_y
Cuts at: 1 417 627 948 1176 1643 1666 2001 2247
Size: 416 210 321 228 467 23 335 246
Cuts at: 2247 3259 3345 3357 3703 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 416 346 335 321 246
228 210 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 281 316 1394 281
Size: 35 1078 3038
Fragments arranged by size:
3038 1078 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 492 527 492
Size: 35 4116
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 804 1736 1910 2301 2328 3673 804
Size: 932 174 391 27 1345 1282
Fragments arranged by size:
1345 1282 932 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 572 933 2174 2384 572
Size: 361 1241 210 2339
Fragments arranged by size:
2339 1241 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 275 851 857 1976 2408 3155 3423 162
Size: 113 576 6 1119 432 747 268 890
Fragments arranged by size:
1119 890 747 576 432 268 113 6
EcoO109I rG'GnC_Cy
Cuts at: 162 1290 162
Size: 1128 3023
EcoRI G'AATT_C
Cuts at: 1003 1155 1003
Size: 152 3999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 141 2170 141
Size: 2029 2122
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1993 2023 2025 2055 1993
Size: 30 2 30 4089
Fragments arranged by size:
4089 30 30 2
HincII GTy'rAC
Cuts at: 201 1190 201
Size: 989 3162
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 976 2822 3006 976
Size: 1846 184 2121
Fragments arranged by size:
2121 1846 184
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 379 394 806 2140 2267 379
Size: 15 412 1334 127 2263
Fragments arranged by size:
2263 1334 412 127 15
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 829 829
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PsiI TTA'TAA
Cuts at: 1100 1100
Size: 4151
PstI C_TGCA'G
Cuts at: 861 1198 1883 861
Size: 337 685 3129
Fragments arranged by size:
3129 685 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 572 2174 2384 572
Size: 1602 210 2339
Fragments arranged by size:
2339 1602 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 461 3668 461
Size: 3207 944
SexAI A'CCwGG_T
Cuts at: 660 660
Size: 4151
SfcI C'TryA_G
Cuts at: 33 323 857 923 1194 1879 2872 3063 33
Size: 290 534 66 271 685 993 191 1121
Fragments arranged by size:
1121 993 685 534 290 271 191 66
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 560 1306 2221 2713 2975 560
Size: 746 915 492 262 1736
Fragments arranged by size:
1736 915 746 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 107 1285 1639 107
Size: 1178 354 2619
Fragments arranged by size:
2619 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 459 3666 459
Size: 3207 944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI