Restriction map for
pF1KB5544
(Circular) (Six-base) MAPSORT of: pF1KB5544.seq Check: 2225 from: 1 to: 7448
pF1KB5544 7448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1188 1188
Size: 7448
AatII G_ACGT'C
Cuts at: 1552 1552
Size: 7448
AccI GT'mk_AC
Cuts at: 522 2896 4376 4486 522
Size: 2374 1480 110 3484
Fragments arranged by size:
3484 2374 1480 110
Acc65I G'GTAC_C
Cuts at: 4464 4464
Size: 7448
AclI AA'CG_TT
Cuts at: 7288 7288
Size: 7448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1490 2252 3067 3403 3503 3638 5273 5705
Size: 1236 762 815 336 100 135 1635 432
Cuts at: 5705 6452 6720 254
Size: 747 268 982
Fragments arranged by size:
1635 1236 982 815 762 747 432 336
268 135 100
AfeI AGC'GCT
Cuts at: 2677 2677
Size: 7448
AflIII A'CryG_T
Cuts at: 4800 5904 6881 4800
Size: 1104 977 5367
Fragments arranged by size:
5367 1104 977
AgeI A'CCGG_T
Cuts at: 5836 5836
Size: 7448
AhdI GACnn_n'nnGTC
Cuts at: 435 435
Size: 7448
AleI CACnn'nnGTG
Cuts at: 885 2131 2618 3287 885
Size: 1246 487 669 5046
Fragments arranged by size:
5046 1246 669 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 262 3305 262
Size: 3043 4405
AlwNI CAG_nnn'CTG
Cuts at: 411 1041 1179 4218 4417 4753 6320 411
Size: 630 138 3039 199 336 1567 1539
Fragments arranged by size:
3039 1567 1539 630 336 199 138
ApaI G_GGCC'C
Cuts at: 219 1600 1799 3271 219
Size: 1381 199 1472 4396
Fragments arranged by size:
4396 1472 1381 199
ApaLI G'TGCA_C
Cuts at: 3280 6218 3280
Size: 2938 4510
ApoI r'AATT_y
Cuts at: 553 3763 4452 6801 7057 553
Size: 3210 689 2349 256 944
Fragments arranged by size:
3210 2349 944 689 256
AseI AT'TA_AT
Cuts at: 20 2828 3725 20
Size: 2808 897 3743
Fragments arranged by size:
3743 2808 897
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7448
AvaI C'yCGr_G
Cuts at: 1530 2531 3202 3677 4468 1530
Size: 1001 671 475 791 4510
Fragments arranged by size:
4510 1001 791 671 475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 852 885 3470 3503 852
Size: 33 2585 33 4797
Fragments arranged by size:
4797 2585 33 33
BamHI G'GATC_C
Cuts at: 750 807 4473 750
Size: 57 3666 3725
Fragments arranged by size:
3725 3666 57
BanI G'GyrC_C
Cuts at: 298 1834 3310 4464 4878 5126 5161 298
Size: 1536 1476 1154 414 248 35 2585
Fragments arranged by size:
2585 1536 1476 1154 414 248 35
BanII G_rGCy'C
Cuts at: 219 589 1600 1799 1976 2627 3271 4462 5492
Size: 370 1011 199 177 651 644 1191 1030
Cuts at: 5492 219
Size: 2175
Fragments arranged by size:
2175 1191 1030 1011 651 644 370 199
177
BbeI G_GCGC'C
Cuts at: 4882 5130 4882
Size: 248 7200
BbsI GAAGACnn'nnnn_
Cuts at: 431 906 1300 1529 2240 2522 2985 431
Size: 475 394 229 711 282 463 4894
Fragments arranged by size:
4894 711 475 463 394 282 229
BbvCI CC'TCA_GC
Cuts at: 1734 3243 1734
Size: 1509 5939
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1635 1669 90
Size: 34 1511 34 5869
Fragments arranged by size:
5869 1511 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5346 6107 7448 5346
Size: 761 1341 5346
Fragments arranged by size:
5346 1341 761
BclI T'GATC_A
Cuts at: 2766 2991 4262 4968 2766
Size: 225 1271 706 5246
Fragments arranged by size:
5246 1271 706 225
BglI GCCn_nnn'nGGC
Cuts at: 1516 1627 1516
Size: 111 7337
BglII A'GATC_T
Cuts at: 4963 7000 4963
Size: 2037 5411
BlpI GC'TnA_GC
Cuts at: 579 4330 4560 579
Size: 3751 230 3467
Fragments arranged by size:
3751 3467 230
Bme1580I G_kGCm'C
Cuts at: 219 1487 1600 1799 1837 2138 3271 3284 4197
Size: 1268 113 199 38 301 1133 13 913
Cuts at: 4197 5073 5166 6222 219
Size: 876 93 1056 1445
Fragments arranged by size:
1445 1268 1133 1056 913 876 301 199
113 93 38 13
BmgBI CAC'GTC
Cuts at: 326 326
Size: 7448
BmrI ACTGGGnnnn_n'
Cuts at: 294 327 570 1129 1598 4660 4741 5076 7220
Size: 33 243 559 469 3062 81 335 2144
Cuts at: 7220 294
Size: 522
Fragments arranged by size:
3062 2144 559 522 469 335 243 81
33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1929 5815 1929
Size: 3886 3562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1227 1590 1775 2285 2412 2934 4240 4301 1227
Size: 363 185 510 127 522 1306 61 4374
Fragments arranged by size:
4374 1306 522 510 363 185 127 61
Bpu10I CC'TnA_GC
Cuts at: 311 1734 3243 311
Size: 1423 1509 4516
Fragments arranged by size:
4516 1509 1423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 4624 5503 5995 6293 970
Size: 3654 879 492 298 2125
Fragments arranged by size:
3654 2125 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 790 915 7152 790
Size: 125 6237 1086
Fragments arranged by size:
6237 1086 125
BsaAI yAC'GTr
Cuts at: 4801 4801
Size: 7448
BsaBI GATnn'nnATC
Cuts at: 6 4962 6999 6
Size: 4956 2037 455
Fragments arranged by size:
4956 2037 455
BsaHI Gr'CG_yC
Cuts at: 1549 4879 5127 5829 7325 1549
Size: 3330 248 702 1496 1672
Fragments arranged by size:
3330 1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 917 2571 4637 5158 5836 6110 6257 6668 917
Size: 1654 2066 521 678 274 147 411 1697
Fragments arranged by size:
2066 1697 1654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1240 1270 1282 1312 1479 1509 2385 2415 1240
Size: 30 12 30 167 30 876 30 6273
Fragments arranged by size:
6273 876 167 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1593 1677 1909 1969 2167 2480 3249 3439 1593
Size: 84 232 60 198 313 769 190 5602
Fragments arranged by size:
5602 769 313 232 198 190 84 60
BseYI C'CCAG_C
Cuts at: 1696 1975 4211 4873 5631 6208 6704 1696
Size: 279 2236 662 758 577 496 2440
Fragments arranged by size:
2440 2236 758 662 577 496 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 268 728 1192 3487 205
Size: 63 460 464 2295 4166
Fragments arranged by size:
4166 2295 464 460 63
BsiEI CG_ry'CG
Cuts at: 104 1730 4990 5036 6244 104
Size: 1626 3260 46 1208 1308
Fragments arranged by size:
3260 1626 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 317 1892 2384 2627 3055 3284 3618 3678
Size: 147 1575 492 243 428 229 334 60
Cuts at: 3678 4462 5240 5430 6222 170
Size: 784 778 190 792 1396
Fragments arranged by size:
1575 1396 792 784 778 492 428 334
243 229 190 147 60
BsmI GAATG_Cn'
Cuts at: 2709 2709
Size: 7448
BsmBI CGTCTCn'nnnn_
Cuts at: 1499 1997 1499
Size: 498 6950
Bsp1286I G_dGCh'C
Cuts at: 170 219 317 589 1487 1600 1799 1837 1892
Size: 49 98 272 898 113 199 38 55
Cuts at: 1892 1976 2138 2384 2627 3055 3271 3284 3618
Size: 84 162 246 243 428 216 13 334
Cuts at: 3618 3678 4197 4462 5073 5166 5240 5430 5492
Size: 60 519 265 611 93 74 190 62
Cuts at: 5492 6222 170
Size: 730 1396
Fragments arranged by size:
1396 898 730 611 519 428 334 272
265 246 243 216 199 190 162 113
98 93 84 74 62 60 55 49
38 13
BspEI T'CCGG_A
Cuts at: 917 2571 6668 917
Size: 1654 4097 1697
Fragments arranged by size:
4097 1697 1654
BspHI T'CATG_A
Cuts at: 786 1241 4155 6624 786
Size: 455 2914 2469 1610
Fragments arranged by size:
2914 2469 1610 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1188 4498 4712 5014 5395 1188
Size: 3310 214 302 381 3241
Fragments arranged by size:
3310 3241 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1884 1959 5740 5794 7278 7443 50
Size: 1834 75 3781 54 1484 165 55
Fragments arranged by size:
3781 1834 1484 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 579 2372 2870 3694 3765 4331 5360 579
Size: 1793 498 824 71 566 1029 2667
Fragments arranged by size:
2667 1793 1029 824 566 498 71
BsrFI r'CCGG_y
Cuts at: 1657 2092 5446 5627 5836 1657
Size: 435 3354 181 209 3269
Fragments arranged by size:
3354 3269 435 209 181
BssSI C'ACGA_G
Cuts at: 223 1891 5719 6077 223
Size: 1668 3828 358 1594
Fragments arranged by size:
3828 1668 1594 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1041 3786 4825 1041
Size: 2745 1039 3664
Fragments arranged by size:
3664 2745 1039
BstBI TT'CG_AA
Cuts at: 5809 5809
Size: 7448
BstEII G'GTnAC_C
Cuts at: 1638 1938 2118 1638
Size: 300 180 6968
Fragments arranged by size:
6968 300 180
BstXI CCAn_nnnn'nTGG
Cuts at: 574 574
Size: 7448
BstYI r'GATC_y
Cuts at: 1 251 750 807 3238 4473 4940 4963 5298
Size: 250 499 57 2431 1235 467 23 335
Cuts at: 5298 5544 6556 6642 6654 7000 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2431 1235 1012 499 467 449 346 335
250 246 86 57 23 12
BtgI C'CryG_G
Cuts at: 704 762 974 1580 2214 704
Size: 58 212 606 634 5938
Fragments arranged by size:
5938 634 606 212 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5398 5579 5742 5398
Size: 181 163 7104
Fragments arranged by size:
7104 181 163
BtsI GCAGTG_nn'
Cuts at: 251 1192 2051 2699 3282 3294 4691 251
Size: 941 859 648 583 12 1397 3008
Fragments arranged by size:
3008 1397 941 859 648 583 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3581 3616 3581
Size: 35 7413
DraI TTT'AAA
Cuts at: 4447 4447
Size: 7448
DraIII CAC_nnn'GTG
Cuts at: 1706 3584 1706
Size: 1878 5570
DrdI GACnn_nn'nnGTC
Cuts at: 505 5154 6012 505
Size: 4649 858 1941
Fragments arranged by size:
4649 1941 858
EaeI y'GGCC_r
Cuts at: 707 1727 3001 3895 5033 5207 5598 5625 6970
Size: 1020 1274 894 1138 174 391 27 1345
Cuts at: 6970 707
Size: 1185
Fragments arranged by size:
1345 1274 1185 1138 1020 894 391 174
27
EagI C'GGCC_G
Cuts at: 1727 5033 1727
Size: 3306 4142
EarI CTCTTCn'nnn_
Cuts at: 1509 1924 3043 3657 4115 5471 5681 1509
Size: 415 1119 614 458 1356 210 3276
Fragments arranged by size:
3276 1356 1119 614 458 415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1056 2093 5656 5966 6112 7258 1056
Size: 1037 3563 310 146 1146 1246
Fragments arranged by size:
3563 1246 1146 1037 310 146
EcoICRI GAG'CTC
Cuts at: 2625 4460 2625
Size: 1835 5613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1227 1490 1590 1775 2252 2285 2412 2934
Size: 973 263 100 185 477 33 127 522
Cuts at: 2934 3067 3403 3503 3638 4240 4301 5273 5705
Size: 133 336 100 135 602 61 972 432
Cuts at: 5705 6452 6720 254
Size: 747 268 982
Fragments arranged by size:
982 973 972 747 602 522 477 432
336 268 263 185 135 133 127 100
100 61 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2795 4131 2300
Size: 495 1336 5617
Fragments arranged by size:
5617 1336 495
EcoO109I rG'GnC_Cy
Cuts at: 215 216 825 1597 1795 2884 3268 4587 215
Size: 1 609 772 198 1089 384 1319 3076
Fragments arranged by size:
3076 1319 1089 772 609 384 198 1
EcoRI G'AATT_C
Cuts at: 4452 4452
Size: 7448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 816 3484 4345 5467 816
Size: 2668 861 1122 2797
Fragments arranged by size:
2797 2668 1122 861
FspI TGC'GCA
Cuts at: 1402 5229 1402
Size: 3827 3621
HaeII r_GCGC'y
Cuts at: 2679 4882 5130 6152 7131 2679
Size: 2203 248 1022 979 2996
Fragments arranged by size:
2996 2203 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 453 2310 2342 3153 3185 3810 3842 3950
Size: 32 1857 32 811 32 625 32 108
Cuts at: 3950 3982 5290 5320 5322 5352 421
Size: 32 1308 30 2 30 2517
Fragments arranged by size:
2517 1857 1308 811 625 108 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 197 3366 3516 4487 197
Size: 3169 150 971 3158
Fragments arranged by size:
3169 3158 971 150
HpaI GTT'AAC
Cuts at: 3366 3516 3366
Size: 150 7298
KasI G'GCGC_C
Cuts at: 4878 5126 4878
Size: 248 7200
KpnI G_GTAC'C
Cuts at: 4468 4468
Size: 7448
MluI A'CGCG_T
Cuts at: 6881 6881
Size: 7448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1369 3437 6119 6303 1369
Size: 2068 2682 184 2514
Fragments arranged by size:
2682 2514 2068 184
MscI TGG'CCA
Cuts at: 709 3003 3897 5209 709
Size: 2294 894 1312 2948
Fragments arranged by size:
2948 2294 1312 894
MslI CAynn'nnrTG
Cuts at: 572 885 1162 1168 2131 2377 2507 2618 2950
Size: 313 277 6 963 246 130 111 332
Cuts at: 2950 3287 3509 3847 5437 5564 572
Size: 337 222 338 1590 127 2456
Fragments arranged by size:
2456 1590 963 338 337 332 313 277
246 222 130 127 111 6
MspA1I CmG'CkG
Cuts at: 338 583 1265 2216 2263 3501 4559 4873 5233
Size: 245 682 951 47 1238 1058 314 360
Cuts at: 5233 6246 6491 338
Size: 1013 245 1295
Fragments arranged by size:
1295 1238 1058 1013 951 682 360 314
245 245 47
NaeI GCC'GGC
Cuts at: 1659 2094 5629 1659
Size: 435 3535 3478
Fragments arranged by size:
3535 3478 435
NarI GG'CG_CC
Cuts at: 4879 5127 4879
Size: 248 7200
NcoI C'CATG_G
Cuts at: 704 1580 704
Size: 876 6572
NdeI CA'TA_TG
Cuts at: 2763 2763
Size: 7448
NgoMIV G'CCGG_C
Cuts at: 1657 2092 5627 1657
Size: 435 3535 3478
Fragments arranged by size:
3535 3478 435
NspI r_CATG'y
Cuts at: 1401 3067 3336 4501 5001 5908 1401
Size: 1666 269 1165 500 907 2941
Fragments arranged by size:
2941 1666 1165 907 500 269
PasI CC'CwG_GG
Cuts at: 843 843
Size: 7448
PciI A'CATG_T
Cuts at: 5904 5904
Size: 7448
PflMI CCAn_nnn'nTGG
Cuts at: 3700 3870 4218 3700
Size: 170 348 6930
Fragments arranged by size:
6930 348 170
PfoI T'CCnGG_A
Cuts at: 821 1081 1204 821
Size: 260 123 7065
Fragments arranged by size:
7065 260 123
PmeI GTTT'AAAC
Cuts at: 4447 4447
Size: 7448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 401 433 3634 3666 6587 6619 401
Size: 32 3201 32 2921 32 1230
Fragments arranged by size:
3201 2921 1230 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 825 2884 825
Size: 2059 5389
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 7448
PspOMI G'GGCC_C
Cuts at: 215 1596 1795 3267 215
Size: 1381 199 1472 4396
Fragments arranged by size:
4396 1472 1381 199
PspXI vC'TCGA_Gb
Cuts at: 2531 3677 2531
Size: 1146 6302
PstI C_TGCA'G
Cuts at: 1199 2706 3289 4495 5180 1199
Size: 1507 583 1206 685 3467
Fragments arranged by size:
3467 1507 1206 685 583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7448
PvuII CAG'CTG
Cuts at: 338 1265 3501 4873 5233 338
Size: 927 2236 1372 360 2553
Fragments arranged by size:
2553 2236 1372 927 360
RsrII CG'GwC_CG
Cuts at: 5643 5643
Size: 7448
SacI G_AGCT'C
Cuts at: 2627 4462 2627
Size: 1835 5613
SacII CC_GC'GG
Cuts at: 2217 2217
Size: 7448
SalI G'TCGA_C
Cuts at: 4485 4485
Size: 7448
SapI GCTCTTCn'nnn_
Cuts at: 3043 5471 5681 3043
Size: 2428 210 4810
Fragments arranged by size:
4810 2428 210
SbfI CC_TGCA'GG
Cuts at: 4495 4495
Size: 7448
ScaI AGT'ACT
Cuts at: 3221 6965 3221
Size: 3744 3704
SfcI C'TryA_G
Cuts at: 33 1195 1260 2702 3285 4491 5176 6169 6360
Size: 1162 65 1442 583 1206 685 993 191
Cuts at: 6360 33
Size: 1121
Fragments arranged by size:
1442 1206 1162 1121 993 685 583 191
65
SfiI GGCCn_nnn'nGGCC
Cuts at: 1627 1627
Size: 7448
SfoI GGC'GCC
Cuts at: 4880 5128 4880
Size: 248 7200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7448
SmaI CCC'GGG
Cuts at: 4470 4470
Size: 7448
SmlI C'TyrA_G
Cuts at: 949 2531 3677 4603 5518 6010 6272 949
Size: 1582 1146 926 915 492 262 2125
Fragments arranged by size:
2125 1582 1146 926 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7448
SphI G_CATG'C
Cuts at: 3336 4501 5001 3336
Size: 1165 500 5783
Fragments arranged by size:
5783 1165 500
SspI AAT'ATT
Cuts at: 1451 1451
Size: 7448
StuI AGG'CCT
Cuts at: 1780 2102 3928 4126 4278 1780
Size: 322 1826 198 152 4950
Fragments arranged by size:
4950 1826 322 198 152
StyI C'CwwG_G
Cuts at: 704 1455 1580 1781 1820 2417 2880 3134 4582
Size: 751 125 201 39 597 463 254 1448
Cuts at: 4582 4936 704
Size: 354 3216
Fragments arranged by size:
3216 1448 751 597 463 354 254 201
125 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1170 1247 1251 2143 2237 2893 5031 5164 5623
Size: 77 4 892 94 656 2138 133 459
Cuts at: 5623 5832 1170
Size: 209 2786
Fragments arranged by size:
2786 2138 892 656 459 209 133 94
77 4
TatI w'GTAC_w
Cuts at: 2680 3219 3431 3890 6963 2680
Size: 539 212 459 3073 3165
Fragments arranged by size:
3165 3073 539 459 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1479 1511 2241 2273 2933 2965 6965 6997 1479
Size: 32 730 32 660 32 4000 32 1930
Fragments arranged by size:
4000 1930 730 660 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 991 2116 4027 5245 991
Size: 1125 1911 1218 3194
Fragments arranged by size:
3194 1911 1218 1125
XbaI T'CTAG_A
Cuts at: 4479 4479
Size: 7448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1779 4943 1779
Size: 3164 4284
XhoI C'TCGA_G
Cuts at: 2531 3677 2531
Size: 1146 6302
XmaI C'CCGG_G
Cuts at: 4468 4468
Size: 7448
XmnI GAAnn'nnTTC
Cuts at: 2552 2588 3464 3651 3805 2552
Size: 36 876 187 154 6195
Fragments arranged by size:
6195 876 187 154 36
ZraI GAC'GTC
Cuts at: 1550 1550
Size: 7448
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BfrBI BmtI BsiWI BsrGI
BssHII BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PsrI SanDI SexAI SgrAI SnaBI SrfI SwaI