Restriction map for
pF1KB5549
(Circular) (Six-base) MAPSORT of: pF1KB5549.seq Check: 2522 from: 1 to: 7253
pF1KB5549 7253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1188 1188
Size: 7253
AccI GT'mk_AC
Cuts at: 2259 3647 4162 4291 2259
Size: 1388 515 129 5221
Fragments arranged by size:
5221 1388 515 129
Acc65I G'GTAC_C
Cuts at: 3902 4269 3902
Size: 367 6886
AclI AA'CG_TT
Cuts at: 7093 7093
Size: 7253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1484 1895 3074 3484 4238 5078 5510 6257 6525
Size: 411 1179 410 754 840 432 747 268
Cuts at: 6525 1484
Size: 2212
Fragments arranged by size:
2212 1179 840 754 747 432 411 410
268
AflIII A'CryG_T
Cuts at: 632 4245 4605 5709 6686 632
Size: 3613 360 1104 977 1199
Fragments arranged by size:
3613 1199 1104 977 360
AgeI A'CCGG_T
Cuts at: 5641 5641
Size: 7253
AleI CACnn'nnGTG
Cuts at: 631 1468 631
Size: 837 6416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3956 3956
Size: 7253
AlwNI CAG_nnn'CTG
Cuts at: 1291 1321 1525 1873 2641 4216 4558 6125 1291
Size: 30 204 348 768 1575 342 1567 2419
Fragments arranged by size:
2419 1575 1567 768 348 342 204 30
ApaI G_GGCC'C
Cuts at: 464 464
Size: 7253
ApaLI G'TGCA_C
Cuts at: 6023 6023
Size: 7253
ApoI r'AATT_y
Cuts at: 1774 1904 2013 2238 3133 3351 4148 4257 6606
Size: 130 109 225 895 218 797 109 2349
Cuts at: 6606 6862 1774
Size: 256 2165
Fragments arranged by size:
2349 2165 895 797 256 225 218 130
109 109
AseI AT'TA_AT
Cuts at: 20 1328 2564 20
Size: 1308 1236 4709
Fragments arranged by size:
4709 1308 1236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7253
AvaI C'yCGr_G
Cuts at: 194 814 2645 4273 194
Size: 620 1831 1628 3174
Fragments arranged by size:
3174 1831 1628 620
BamHI G'GATC_C
Cuts at: 839 4278 839
Size: 3439 3814
BanI G'GyrC_C
Cuts at: 247 810 3902 4269 4683 4931 4966 247
Size: 563 3092 367 414 248 35 2534
Fragments arranged by size:
3092 2534 563 414 367 248 35
BanII G_rGCy'C
Cuts at: 190 464 3240 4267 5297 190
Size: 274 2776 1027 1030 2146
Fragments arranged by size:
2776 2146 1030 1027 274
BbeI G_GCGC'C
Cuts at: 251 4687 4935 251
Size: 4436 248 2569
Fragments arranged by size:
4436 2569 248
BbsI GAAGACnn'nnnn_
Cuts at: 1052 1052
Size: 7253
BbvCI CC'TCA_GC
Cuts at: 310 310
Size: 7253
BciVI GTATCCnnnnn_n'
Cuts at: 2288 3094 3291 5151 5912 7253 2288
Size: 806 197 1860 761 1341 2288
Fragments arranged by size:
2288 1860 1341 806 761 197
BclI T'GATC_A
Cuts at: 1226 4773 1226
Size: 3547 3706
BfrBI ATG'CAT
Cuts at: 522 1797 522
Size: 1275 5978
BglI GCCn_nnn'nGGC
Cuts at: 138 141 199 256 338 492 138
Size: 3 58 57 82 154 6899
Fragments arranged by size:
6899 154 82 58 57 3
BglII A'GATC_T
Cuts at: 3712 4768 6805 3712
Size: 1056 2037 4160
Fragments arranged by size:
4160 2037 1056
BlpI GC'TnA_GC
Cuts at: 4365 4365
Size: 7253
Bme1580I G_kGCm'C
Cuts at: 464 678 815 4878 4971 6027 464
Size: 214 137 4063 93 1056 1690
Fragments arranged by size:
4063 1690 1056 214 137 93
BmgBI CAC'GTC
Cuts at: 633 633
Size: 7253
BmrI ACTGGGnnnn_n'
Cuts at: 1421 2566 3515 4465 4546 4881 7025 1421
Size: 1145 949 950 81 335 2144 1649
Fragments arranged by size:
2144 1649 1145 950 949 335 81
BmtI G_CTAG'C
Cuts at: 1541 2771 1541
Size: 1230 6023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5620 5620
Size: 7253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2612 902
Size: 1710 5543
Bpu10I CC'TnA_GC
Cuts at: 310 4117 4186 310
Size: 3807 69 3377
Fragments arranged by size:
3807 3377 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1665 2437 3224 4429 5308 5800 6098 1665
Size: 772 787 1205 879 492 298 2820
Fragments arranged by size:
2820 1205 879 787 772 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 526 3325 6957 526
Size: 2799 3632 822
Fragments arranged by size:
3632 2799 822
BsaAI yAC'GTr
Cuts at: 4606 4606
Size: 7253
BsaBI GATnn'nnATC
Cuts at: 6 4767 6804 6
Size: 4761 2037 455
Fragments arranged by size:
4761 2037 455
BsaHI Gr'CG_yC
Cuts at: 248 2492 2604 4684 4932 5634 7130 248
Size: 2244 112 2080 248 702 1496 371
Fragments arranged by size:
2244 2080 1496 702 371 248 112
BsaWI w'CCGG_w
Cuts at: 3633 3980 4442 4963 5641 5915 6062 6473 3633
Size: 347 462 521 678 274 147 411 4413
Fragments arranged by size:
4413 678 521 462 411 347 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 286
Size: 30 7223
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 2862 2865 3572 307
Size: 2555 3 707 3988
Fragments arranged by size:
3988 2555 707 3
BseYI C'CCAG_C
Cuts at: 180 646 3162 4678 5436 6013 6509 180
Size: 466 2516 1516 758 577 496 924
Fragments arranged by size:
2516 1516 924 758 577 496 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3662 3662
Size: 7253
BsiEI CG_ry'CG
Cuts at: 104 120 133 260 276 326 366 385 492
Size: 16 13 127 16 50 40 19 107
Cuts at: 492 581 729 747 4795 4841 6049 104
Size: 89 148 18 4048 46 1208 1308
Fragments arranged by size:
4048 1308 1208 148 127 107 89 50
46 40 19 18 16 16 13
BsiHKAI G_wGCw'C
Cuts at: 190 2995 3240 4267 5045 5235 6027 190
Size: 2805 245 1027 778 190 792 1416
Fragments arranged by size:
2805 1416 1027 792 778 245 190
BsmI GAATG_Cn'
Cuts at: 1308 2348 1308
Size: 1040 6213
BsmBI CGTCTCn'nnnn_
Cuts at: 2611 2611
Size: 7253
Bsp1286I G_dGCh'C
Cuts at: 190 464 678 815 2995 3240 4267 4878 4971
Size: 274 214 137 2180 245 1027 611 93
Cuts at: 4971 5045 5235 5297 6027 190
Size: 74 190 62 730 1416
Fragments arranged by size:
2180 1416 1027 730 611 274 245 214
190 137 93 74 62
BspEI T'CCGG_A
Cuts at: 3633 6473 3633
Size: 2840 4413
BspHI T'CATG_A
Cuts at: 3936 4128 6429 3936
Size: 192 2301 4760
Fragments arranged by size:
4760 2301 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 647 1188 2123 4303 4517 4819 5200 647
Size: 541 935 2180 214 302 381 2700
Fragments arranged by size:
2700 2180 935 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 116 129 368 5545 5599 7083 7248 50
Size: 66 13 239 5177 54 1484 165 55
Fragments arranged by size:
5177 1484 239 165 66 55 54 13
BsrDI GCAATG_nn'
Cuts at: 5165 5165
Size: 7253
BsrFI r'CCGG_y
Cuts at: 380 409 491 580 784 4165 5251 5432 5641
Size: 29 82 89 204 3381 1086 181 209
Cuts at: 5641 380
Size: 1992
Fragments arranged by size:
3381 1992 1086 209 204 181 89 82
29
BssHII G'CGCG_C
Cuts at: 822 822
Size: 7253
BssSI C'ACGA_G
Cuts at: 3281 5524 5882 3281
Size: 2243 358 4652
Fragments arranged by size:
4652 2243 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4630 4630
Size: 7253
BstBI TT'CG_AA
Cuts at: 5614 5614
Size: 7253
BstXI CCAn_nnnn'nTGG
Cuts at: 1317 1470 2438 3510 1317
Size: 153 968 1072 5060
Fragments arranged by size:
5060 1072 968 153
BstYI r'GATC_y
Cuts at: 1 839 1288 2043 3712 4278 4745 4768 5103
Size: 838 449 755 1669 566 467 23 335
Cuts at: 5103 5349 6361 6447 6459 6805 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1669 1012 838 755 566 467 449 449
346 335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 3648 3648
Size: 7253
Bsu36I CC'TnA_GG
Cuts at: 431 431
Size: 7253
BtgI C'CryG_G
Cuts at: 107 326 591 3087 107
Size: 219 265 2496 4273
Fragments arranged by size:
4273 2496 265 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3876 5203 5384 5547 3876
Size: 1327 181 163 5582
Fragments arranged by size:
5582 1327 181 163
BtsI GCAGTG_nn'
Cuts at: 4496 4496
Size: 7253
ClaI AT'CG_AT
Cuts at: 2901 2901
Size: 7253
DraI TTT'AAA
Cuts at: 972 4252 972
Size: 3280 3973
DraIII CAC_nnn'GTG
Cuts at: 2574 2574
Size: 7253
DrdI GACnn_nn'nnGTC
Cuts at: 1876 4959 5817 1876
Size: 3083 858 3312
Fragments arranged by size:
3312 3083 858
EaeI y'GGCC_r
Cuts at: 117 130 257 323 363 382 489 578 726
Size: 13 127 66 40 19 107 89 148
Cuts at: 726 744 3624 4838 5012 5403 5430 6775 117
Size: 18 2880 1214 174 391 27 1345 595
Fragments arranged by size:
2880 1345 1214 595 391 174 148 127
107 89 66 40 27 19 18 13
EagI C'GGCC_G
Cuts at: 117 130 257 323 363 382 489 578 726
Size: 13 127 66 40 19 107 89 148
Cuts at: 726 744 4838 117
Size: 18 4094 2532
Fragments arranged by size:
4094 2532 148 127 107 89 66 40
19 18 13
EarI CTCTTCn'nnn_
Cuts at: 284 1812 3722 4214 5276 5486 284
Size: 1528 1910 492 1062 210 2051
Fragments arranged by size:
2051 1910 1528 1062 492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 601 5461 5771 5917 7063 232
Size: 369 4860 310 146 1146 422
Fragments arranged by size:
4860 1146 422 369 310 146
EcoICRI GAG'CTC
Cuts at: 188 3238 4265 188
Size: 3050 1027 3176
Fragments arranged by size:
3176 3050 1027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1484 1895 2612 3074 3484 4238 5078 5510
Size: 582 411 717 462 410 754 840 432
Cuts at: 5510 6257 6525 902
Size: 747 268 1630
Fragments arranged by size:
1630 840 754 747 717 582 462 432
411 410 268
EcoNI CCTnn'n_nnAGG
Cuts at: 805 805
Size: 7253
EcoO109I rG'GnC_Cy
Cuts at: 445 597 4392 445
Size: 152 3795 3306
Fragments arranged by size:
3795 3306 152
EcoRI G'AATT_C
Cuts at: 1904 4257 1904
Size: 2353 4900
EcoRV GAT'ATC
Cuts at: 1736 1736
Size: 7253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 920 4122 5272 920
Size: 3202 1150 2901
Fragments arranged by size:
3202 2901 1150
FseI GG_CCGG'CC
Cuts at: 384 495 384
Size: 111 7142
FspI TGC'GCA
Cuts at: 5034 5034
Size: 7253
HaeII r_GCGC'y
Cuts at: 251 310 2696 3868 4687 4935 5957 6936 251
Size: 59 2386 1172 819 248 1022 979 568
Fragments arranged by size:
2386 1172 1022 979 819 568 248 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 523 555 1513 1545 2028 2060 2447
Size: 32 205 32 958 32 483 32 387
Cuts at: 2447 2479 5095 5125 5127 5157 286
Size: 32 2616 30 2 30 2382
Fragments arranged by size:
2616 2382 958 483 387 205 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2139 2706 3183 4292 2139
Size: 567 477 1109 5100
Fragments arranged by size:
5100 1109 567 477
HindIII A'AGCT_T
Cuts at: 762 986 762
Size: 224 7029
HpaI GTT'AAC
Cuts at: 2139 2139
Size: 7253
KasI G'GCGC_C
Cuts at: 247 4683 4931 247
Size: 4436 248 2569
Fragments arranged by size:
4436 2569 248
KpnI G_GTAC'C
Cuts at: 3906 4273 3906
Size: 367 6886
MfeI C'AATT_G
Cuts at: 3175 3175
Size: 7253
MluI A'CGCG_T
Cuts at: 6686 6686
Size: 7253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 1794 2232 2821 3084 5924 6108 278
Size: 1516 438 589 263 2840 184 1423
Fragments arranged by size:
2840 1516 1423 589 438 263 184
MscI TGG'CCA
Cuts at: 3626 5014 3626
Size: 1388 5865
MslI CAynn'nnrTG
Cuts at: 631 1088 1255 1315 1468 1802 3092 3508 3938
Size: 457 167 60 153 334 1290 416 430
Cuts at: 3938 4100 4130 5242 5369 631
Size: 162 30 1112 127 2515
Fragments arranged by size:
2515 1290 1112 457 430 416 334 167
162 153 127 60 30
MspA1I CmG'CkG
Cuts at: 328 387 480 593 725 2839 3166 4364 4678
Size: 59 93 113 132 2114 327 1198 314
Cuts at: 4678 5038 6051 6296 328
Size: 360 1013 245 1285
Fragments arranged by size:
2114 1285 1198 1013 360 327 314 245
132 113 93 59
NaeI GCC'GGC
Cuts at: 382 411 493 582 5434 382
Size: 29 82 89 4852 2201
Fragments arranged by size:
4852 2201 89 82 29
NarI GG'CG_CC
Cuts at: 248 4684 4932 248
Size: 4436 248 2569
Fragments arranged by size:
4436 2569 248
NcoI C'CATG_G
Cuts at: 107 3087 107
Size: 2980 4273
NgoMIV G'CCGG_C
Cuts at: 380 409 491 580 5432 380
Size: 29 82 89 4852 2201
Fragments arranged by size:
4852 2201 89 82 29
NheI G'CTAG_C
Cuts at: 1537 2767 1537
Size: 1230 6023
NotI GC'GGCC_GC
Cuts at: 130 323 726 744 130
Size: 193 403 18 6639
Fragments arranged by size:
6639 403 193 18
NsiI A_TGCA'T
Cuts at: 524 1799 524
Size: 1275 5978
NspI r_CATG'y
Cuts at: 522 4249 4306 4806 5713 522
Size: 3727 57 500 907 2062
Fragments arranged by size:
3727 2062 907 500 57
PacI TTA_AT'TAA
Cuts at: 2825 2825
Size: 7253
PciI A'CATG_T
Cuts at: 4245 5709 4245
Size: 1464 5789
PfoI T'CCnGG_A
Cuts at: 1035 1380 2616 3552 4199 1035
Size: 345 1236 936 647 4089
Fragments arranged by size:
4089 1236 936 647 345
PmeI GTTT'AAAC
Cuts at: 4252 4252
Size: 7253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 253 285 2489 2521 6392 6424 253
Size: 32 2204 32 3871 32 1082
Fragments arranged by size:
3871 2204 1082 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 597 597
Size: 7253
PshAI GACnn'nnGTC
Cuts at: 737 3374 737
Size: 2637 4616
PsiI TTA'TAA
Cuts at: 2627 2627
Size: 7253
PspOMI G'GGCC_C
Cuts at: 460 460
Size: 7253
PspXI vC'TCGA_Gb
Cuts at: 2645 2645
Size: 7253
PstI C_TGCA'G
Cuts at: 4300 4985 4300
Size: 685 6568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7253
PvuII CAG'CTG
Cuts at: 3166 4678 5038 3166
Size: 1512 360 5381
Fragments arranged by size:
5381 1512 360
RsrII CG'GwC_CG
Cuts at: 5448 5448
Size: 7253
SacI G_AGCT'C
Cuts at: 190 3240 4267 190
Size: 3050 1027 3176
Fragments arranged by size:
3176 3050 1027
SacII CC_GC'GG
Cuts at: 329 594 329
Size: 265 6988
SalI G'TCGA_C
Cuts at: 4290 4290
Size: 7253
SanDI GG'GwC_CC
Cuts at: 597 597
Size: 7253
SapI GCTCTTCn'nnn_
Cuts at: 5276 5486 5276
Size: 210 7043
SbfI CC_TGCA'GG
Cuts at: 4300 4300
Size: 7253
ScaI AGT'ACT
Cuts at: 6770 6770
Size: 7253
SexAI A'CCwGG_T
Cuts at: 1761 1761
Size: 7253
SfcI C'TryA_G
Cuts at: 33 942 4296 4981 5974 6165 33
Size: 909 3354 685 993 191 1121
Fragments arranged by size:
3354 1121 993 909 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 338 338
Size: 7253
SfoI GGC'GCC
Cuts at: 249 4685 4933 249
Size: 4436 248 2569
Fragments arranged by size:
4436 2569 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7253
SgrAI Cr'CCGG_yG
Cuts at: 784 784
Size: 7253
SmaI CCC'GGG
Cuts at: 196 4275 196
Size: 4079 3174
SmlI C'TyrA_G
Cuts at: 1644 2416 2645 3239 4408 5323 5815 6077 1644
Size: 772 229 594 1169 915 492 262 2820
Fragments arranged by size:
2820 1169 915 772 594 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7253
SphI G_CATG'C
Cuts at: 4306 4806 4306
Size: 500 6753
SrfI GCCC'GGGC
Cuts at: 196 196
Size: 7253
SspI AAT'ATT
Cuts at: 1560 2290 3549 1560
Size: 730 1259 5264
Fragments arranged by size:
5264 1259 730
StyI C'CwwG_G
Cuts at: 107 136 1691 2231 3087 3997 4387 4741 107
Size: 29 1555 540 856 910 390 354 2619
Fragments arranged by size:
2619 1555 910 856 540 390 354 29
SwaI ATTT'AAAT
Cuts at: 972 972
Size: 7253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 2585 4836 4969 5428 5637 381
Size: 2204 2251 133 459 209 1997
Fragments arranged by size:
2251 2204 1997 459 209 133
TatI w'GTAC_w
Cuts at: 1781 2100 3253 3435 3804 3855 6768 1781
Size: 319 1153 182 369 51 2913 2266
Fragments arranged by size:
2913 2266 1153 369 319 182 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1793 1825 2998 3030 6770 6802 1793
Size: 32 1173 32 3740 32 2244
Fragments arranged by size:
3740 2244 1173 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 537 5050 537
Size: 4513 2740
XbaI T'CTAG_A
Cuts at: 2508 4284 2508
Size: 1776 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4748 4748
Size: 7253
XhoI C'TCGA_G
Cuts at: 2645 2645
Size: 7253
XmaI C'CCGG_G
Cuts at: 194 4273 194
Size: 4079 3174
XmnI GAAnn'nnTTC
Cuts at: 1244 2291 1244
Size: 1047 6206
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NsiI
NspI PacI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BsiWI BsrGI BstEII CspCI FspAI NdeI NruI
PasI PflMI PmlI PsrI SnaBI StuI ZraI