Restriction map for
pF1KB5550
(Circular) (Six-base) MAPSORT of: pF1KB5550.seq Check: 2203 from: 1 to: 5075
pF1KB5550 5075 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1117 2113 1117
Size: 996 4079
Acc65I G'GTAC_C
Cuts at: 2091 2091
Size: 5075
AclI AA'CG_TT
Cuts at: 4915 4915
Size: 5075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 554 1667 1733 1892 2900 3332 3685 4347
Size: 216 1113 66 159 1008 432 353 662
Cuts at: 4347 338
Size: 1066
Fragments arranged by size:
1113 1066 1008 662 432 353 216 159
66
AflIII A'CryG_T
Cuts at: 2427 4227 4508 2427
Size: 1800 281 2994
Fragments arranged by size:
2994 1800 281
AleI CACnn'nnGTG
Cuts at: 1432 1432
Size: 5075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 430 462 430
Size: 32 5043
AlwNI CAG_nnn'CTG
Cuts at: 1227 1471 2380 3818 1227
Size: 244 909 1438 2484
Fragments arranged by size:
2484 1438 909 244
ApaI G_GGCC'C
Cuts at: 1002 1002
Size: 5075
ApaLI G'TGCA_C
Cuts at: 3913 3913
Size: 5075
ApoI r'AATT_y
Cuts at: 539 1084 1208 1565 2079 4428 4684 539
Size: 545 124 357 514 2349 256 930
Fragments arranged by size:
2349 930 545 514 357 256 124
AseI AT'TA_AT
Cuts at: 20 20
Size: 5075
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
AvaI C'yCGr_G
Cuts at: 370 1076 2095 370
Size: 706 1019 3350
Fragments arranged by size:
3350 1019 706
BamHI G'GATC_C
Cuts at: 2100 2100
Size: 5075
BanI G'GyrC_C
Cuts at: 1434 2091 2505 2753 2788 1434
Size: 657 414 248 35 3721
Fragments arranged by size:
3721 657 414 248 35
BanII G_rGCy'C
Cuts at: 1002 1097 2089 3119 1002
Size: 95 992 1030 2958
Fragments arranged by size:
2958 1030 992 95
BbeI G_GCGC'C
Cuts at: 2509 2757 2509
Size: 248 4827
BbsI GAAGACnn'nnnn_
Cuts at: 822 881 931 998 1061 1655 1766 1955 822
Size: 59 50 67 63 594 111 189 3942
Fragments arranged by size:
3942 594 189 111 67 63 59 50
BbvCI CC'TCA_GC
Cuts at: 578 1754 578
Size: 1176 3899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 120 154 180 214 120
Size: 34 26 34 4981
Fragments arranged by size:
4981 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2973 4029 5075 2040
Size: 933 1056 1046 2040
Fragments arranged by size:
2040 1056 1046 933
BclI T'GATC_A
Cuts at: 1329 2595 1329
Size: 1266 3809
BglI GCCn_nnn'nGGC
Cuts at: 997 997
Size: 5075
BglII A'GATC_T
Cuts at: 1454 2590 4627 1454
Size: 1136 2037 1902
Fragments arranged by size:
2037 1902 1136
BlpI GC'TnA_GC
Cuts at: 145 2187 145
Size: 2042 3033
Bme1580I G_kGCm'C
Cuts at: 193 1002 2700 2793 3917 193
Size: 809 1698 93 1124 1351
Fragments arranged by size:
1698 1351 1124 809 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 5075
BmrI ACTGGGnnnn_n'
Cuts at: 2287 2368 2703 4847 2287
Size: 81 335 2144 2515
Fragments arranged by size:
2515 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5075
Bpu10I CC'TnA_GC
Cuts at: 578 1754 578
Size: 1176 3899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1756 2251 3130 3844 4142 1756
Size: 495 879 714 298 2689
Fragments arranged by size:
2689 879 714 495 298
BsaI GGTCTCn'nnnn_
Cuts at: 1060 1876 4779 1060
Size: 816 2903 1356
Fragments arranged by size:
2903 1356 816
BsaAI yAC'GTr
Cuts at: 413 2428 413
Size: 2015 3060
BsaBI GATnn'nnATC
Cuts at: 6 860 1681 2589 4626 6
Size: 854 821 908 2037 455
Fragments arranged by size:
2037 908 854 821 455
BsaHI Gr'CG_yC
Cuts at: 389 2506 2754 3456 4952 389
Size: 2117 248 702 1496 512
Fragments arranged by size:
2117 1496 702 512 248
BsaWI w'CCGG_w
Cuts at: 1112 2264 2785 3463 3874 4021 4295 1112
Size: 1152 521 678 411 147 274 1892
Fragments arranged by size:
1892 1152 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 513 543 513
Size: 30 5045
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 181 1568 178
Size: 3 1387 3685
Fragments arranged by size:
3685 1387 3
BseYI C'CCAG_C
Cuts at: 1851 2500 3258 3923 4331 1851
Size: 649 758 665 408 2595
Fragments arranged by size:
2595 758 665 649 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 982 2039 451
Size: 531 1057 3487
Fragments arranged by size:
3487 1057 531
BsiEI CG_ry'CG
Cuts at: 104 252 408 2617 2663 3893 104
Size: 148 156 2209 46 1230 1286
Fragments arranged by size:
2209 1286 1230 156 148 46
BsiHKAI G_wGCw'C
Cuts at: 1097 1322 2089 2867 3057 3917 1097
Size: 225 767 778 190 860 2255
Fragments arranged by size:
2255 860 778 767 225 190
Bsp1286I G_dGCh'C
Cuts at: 193 1002 1097 1322 2089 2700 2793 2867 3057
Size: 809 95 225 767 611 93 74 190
Cuts at: 3057 3119 3917 193
Size: 62 798 1351
Fragments arranged by size:
1351 809 798 767 611 225 190 95
93 74 62
BspHI T'CATG_A
Cuts at: 1403 3507 1403
Size: 2104 2971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2125 2339 2641 3022 2125
Size: 214 302 381 4178
Fragments arranged by size:
4178 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3367 3421 4905 5070 50
Size: 3317 54 1484 165 55
Fragments arranged by size:
3317 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2987 2987
Size: 5075
BsrFI r'CCGG_y
Cuts at: 3073 3254 3073
Size: 181 4894
BsrGI T'GTAC_A
Cuts at: 914 914
Size: 5075
BssSI C'ACGA_G
Cuts at: 1580 3346 4054 1580
Size: 1766 708 2601
Fragments arranged by size:
2601 1766 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2452 2452
Size: 5075
BstBI TT'CG_AA
Cuts at: 833 3436 833
Size: 2603 2472
BstEII G'GTnAC_C
Cuts at: 600 1342 600
Size: 742 4333
BstYI r'GATC_y
Cuts at: 1 1104 1454 2100 2567 2590 2925 3171 3477
Size: 1103 350 646 467 23 335 246 306
Cuts at: 3477 3489 3575 4627 1
Size: 12 86 1052 449
Fragments arranged by size:
1103 1052 646 467 449 350 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 548 1263 1886 548
Size: 715 623 3737
Fragments arranged by size:
3737 715 623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 145 249 353 3025 3206 3369 145
Size: 104 104 2672 181 163 1851
Fragments arranged by size:
2672 1851 181 163 104 104
BtsI GCAGTG_nn'
Cuts at: 2318 2318
Size: 5075
DraI TTT'AAA
Cuts at: 2074 2074
Size: 5075
DrdI GACnn_nn'nnGTC
Cuts at: 826 889 2781 4125 826
Size: 63 1892 1344 1776
Fragments arranged by size:
1892 1776 1344 63
EaeI y'GGCC_r
Cuts at: 125 249 405 842 2660 2834 3225 3252 4597
Size: 124 156 437 1818 174 391 27 1345
Cuts at: 4597 125
Size: 603
Fragments arranged by size:
1818 1345 603 437 391 174 156 124
27
EagI C'GGCC_G
Cuts at: 249 405 2660 249
Size: 156 2255 2664
Fragments arranged by size:
2664 2255 156
EarI CTCTTCn'nnn_
Cuts at: 812 1085 2036 3098 3308 812
Size: 273 951 1062 210 2579
Fragments arranged by size:
2579 1062 951 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 803 3283 4025 4171 4885 803
Size: 2480 742 146 714 993
Fragments arranged by size:
2480 993 742 714 146
EcoICRI GAG'CTC
Cuts at: 1095 2087 1095
Size: 992 4083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 554 1667 1733 1892 2900 3332 3685 4347
Size: 216 1113 66 159 1008 432 353 662
Cuts at: 4347 338
Size: 1066
Fragments arranged by size:
1113 1066 1008 662 432 353 216 159
66
EcoO109I rG'GnC_Cy
Cuts at: 999 1587 2023 2214 999
Size: 588 436 191 3860
Fragments arranged by size:
3860 588 436 191
EcoRI G'AATT_C
Cuts at: 1208 1565 2079 1208
Size: 357 514 4204
Fragments arranged by size:
4204 514 357
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 5075
FspI TGC'GCA
Cuts at: 2856 2856
Size: 5075
HaeII r_GCGC'y
Cuts at: 2509 2757 3987 4758 2509
Size: 248 1230 771 2826
Fragments arranged by size:
2826 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 513 545 1059 1091 2917 2947 2949 2979 513
Size: 32 514 32 1826 30 2 30 2609
Fragments arranged by size:
2609 1826 514 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2114 2114
Size: 5075
KasI G'GCGC_C
Cuts at: 2505 2753 2505
Size: 248 4827
KpnI G_GTAC'C
Cuts at: 2095 2095
Size: 5075
MluI A'CGCG_T
Cuts at: 4508 4508
Size: 5075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 1124 1781 3834 4018 389
Size: 735 657 2053 184 1446
Fragments arranged by size:
2053 1446 735 657 184
MscI TGG'CCA
Cuts at: 844 2836 844
Size: 1992 3083
MslI CAynn'nnrTG
Cuts at: 562 769 866 1432 1934 3064 3191 562
Size: 207 97 566 502 1130 127 2446
Fragments arranged by size:
2446 1130 566 502 207 127 97
MspA1I CmG'CkG
Cuts at: 577 721 1645 2186 2500 2860 3644 3889 577
Size: 144 924 541 314 360 784 245 1763
Fragments arranged by size:
1763 924 784 541 360 314 245 144
NaeI GCC'GGC
Cuts at: 3256 3256
Size: 5075
NarI GG'CG_CC
Cuts at: 2506 2754 2506
Size: 248 4827
NcoI C'CATG_G
Cuts at: 548 1886 548
Size: 1338 3737
NgoMIV G'CCGG_C
Cuts at: 3254 3254
Size: 5075
NspI r_CATG'y
Cuts at: 2128 2628 4231 2128
Size: 500 1603 2972
Fragments arranged by size:
2972 1603 500
PciI A'CATG_T
Cuts at: 4227 4227
Size: 5075
PflMI CCAn_nnn'nTGG
Cuts at: 1264 1264
Size: 5075
PmeI GTTT'AAAC
Cuts at: 2074 2074
Size: 5075
PmlI CAC'GTG
Cuts at: 413 413
Size: 5075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 5043
PpuMI rG'GwC_Cy
Cuts at: 1587 1587
Size: 5075
PspOMI G'GGCC_C
Cuts at: 998 998
Size: 5075
PstI C_TGCA'G
Cuts at: 2122 2807 2122
Size: 685 4390
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5075
PvuII CAG'CTG
Cuts at: 721 1645 2500 2860 721
Size: 924 855 360 2936
Fragments arranged by size:
2936 924 855 360
RsrII CG'GwC_CG
Cuts at: 3270 3270
Size: 5075
SacI G_AGCT'C
Cuts at: 1097 2089 1097
Size: 992 4083
SalI G'TCGA_C
Cuts at: 2112 2112
Size: 5075
SanDI GG'GwC_CC
Cuts at: 1587 1587
Size: 5075
SapI GCTCTTCn'nnn_
Cuts at: 1085 3098 3308 1085
Size: 2013 210 2852
Fragments arranged by size:
2852 2013 210
SbfI CC_TGCA'GG
Cuts at: 2122 2122
Size: 5075
ScaI AGT'ACT
Cuts at: 4592 4592
Size: 5075
SexAI A'CCwGG_T
Cuts at: 1346 1346
Size: 5075
SfcI C'TryA_G
Cuts at: 33 1286 1463 1898 2118 2803 3771 3962 33
Size: 1253 177 435 220 685 968 191 1146
Fragments arranged by size:
1253 1146 968 685 435 220 191 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 997 997
Size: 5075
SfoI GGC'GCC
Cuts at: 2507 2755 2507
Size: 248 4827
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
SmaI CCC'GGG
Cuts at: 2097 2097
Size: 5075
SmlI C'TyrA_G
Cuts at: 1771 2230 3145 3859 4121 1771
Size: 459 915 714 262 2725
Fragments arranged by size:
2725 915 714 459 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5075
SphI G_CATG'C
Cuts at: 2128 2628 2128
Size: 500 4575
StyI C'CwwG_G
Cuts at: 548 1420 1886 2209 2563 548
Size: 872 466 323 354 3060
Fragments arranged by size:
3060 872 466 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 772 856 2658 2791 3250 3459 772
Size: 84 1802 133 459 209 2388
Fragments arranged by size:
2388 1802 459 209 133 84
TatI w'GTAC_w
Cuts at: 914 4590 914
Size: 3676 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4592 4624 4592
Size: 32 5043
Tth111I GACn'n_nGTC
Cuts at: 2872 2872
Size: 5075
XbaI T'CTAG_A
Cuts at: 2106 2106
Size: 5075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 2570
Size: 5075
XmaI C'CCGG_G
Cuts at: 2095 2095
Size: 5075
XmnI GAAnn'nnTTC
Cuts at: 1184 1985 1184
Size: 801 4274
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
AvrII BaeI BfrBI BmtI BpmI BsiWI BsmI BsmBI
BspEI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspXI PsrI SacII SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI