Restriction map for
pF1KB5551
(Circular) (Six-base) MAPSORT of: pF1KB5551.seq Check: 450 from: 1 to: 5153
pF1KB5551 5153 bp
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1946 1946
Size: 5153
AatII G_ACGT'C
Cuts at: 1148 1148
Size: 5153
AccI GT'mk_AC
Cuts at: 1925 2191 1925
Size: 266 4887
Acc65I G'GTAC_C
Cuts at: 2169 2169
Size: 5153
AclI AA'CG_TT
Cuts at: 4993 4993
Size: 5153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 934 2978 3410 4157 4425 231
Size: 703 2044 432 747 268 959
Fragments arranged by size:
2044 959 747 703 432 268
AflIII A'CryG_T
Cuts at: 2138 2505 3609 4586 2138
Size: 367 1104 977 2705
Fragments arranged by size:
2705 1104 977 367
AgeI A'CCGG_T
Cuts at: 3541 3541
Size: 5153
AhdI GACnn_n'nnGTC
Cuts at: 1419 1419
Size: 5153
AleI CACnn'nnGTG
Cuts at: 1448 1529 1723 1448
Size: 81 194 4878
Fragments arranged by size:
4878 194 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 677 1547 677
Size: 870 4283
AlwNI CAG_nnn'CTG
Cuts at: 1317 1938 2458 4025 1317
Size: 621 520 1567 2445
Fragments arranged by size:
2445 1567 621 520
ApaI G_GGCC'C
Cuts at: 1714 1714
Size: 5153
ApaLI G'TGCA_C
Cuts at: 1522 3923 1522
Size: 2401 2752
ApoI r'AATT_y
Cuts at: 284 2157 4506 4762 284
Size: 1873 2349 256 675
Fragments arranged by size:
2349 1873 675 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5153
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
AvaI C'yCGr_G
Cuts at: 271 1904 2065 2173 271
Size: 1633 161 108 3251
Fragments arranged by size:
3251 1633 161 108
BamHI G'GATC_C
Cuts at: 2178 2178
Size: 5153
BanI G'GyrC_C
Cuts at: 703 1132 1641 1717 1728 1984 2169 2583 2831
Size: 429 509 76 11 256 185 414 248
Cuts at: 2831 2866 703
Size: 35 2990
Fragments arranged by size:
2990 509 429 414 256 248 185 76
35 11
BanII G_rGCy'C
Cuts at: 1304 1714 2066 2167 3197 1304
Size: 410 352 101 1030 3260
Fragments arranged by size:
3260 1030 410 352 101
BbeI G_GCGC'C
Cuts at: 707 2587 2835 707
Size: 1880 248 3025
Fragments arranged by size:
3025 1880 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 893 1966 2000 859
Size: 34 1073 34 4012
Fragments arranged by size:
4012 1073 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3051 3812 5153 3051
Size: 761 1341 3051
Fragments arranged by size:
3051 1341 761
BclI T'GATC_A
Cuts at: 110 2673 110
Size: 2563 2590
BglII A'GATC_T
Cuts at: 2668 4705 2668
Size: 2037 3116
BlpI GC'TnA_GC
Cuts at: 2060 2265 2060
Size: 205 4948
Bme1580I G_kGCm'C
Cuts at: 1207 1526 1646 1714 1989 2778 2871 3927 1207
Size: 319 120 68 275 789 93 1056 2433
Fragments arranged by size:
2433 1056 789 319 275 120 93 68
BmgBI CAC'GTC
Cuts at: 597 2139 597
Size: 1542 3611
BmrI ACTGGGnnnn_n'
Cuts at: 1659 2365 2446 2781 4925 1659
Size: 706 81 335 2144 1887
Fragments arranged by size:
2144 1887 706 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3520
Size: 5153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 712
Size: 5153
Bpu10I CC'TnA_GC
Cuts at: 481 1366 481
Size: 885 4268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2329 3208 3700 3998 2329
Size: 879 492 298 3484
Fragments arranged by size:
3484 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 539 1546 4857 229
Size: 310 1007 3311 525
Fragments arranged by size:
3311 1007 525 310
BsaAI yAC'GTr
Cuts at: 132 678 1074 2506 132
Size: 546 396 1432 2779
Fragments arranged by size:
2779 1432 546 396
BsaBI GATnn'nnATC
Cuts at: 6 2667 4704 6
Size: 2661 2037 455
Fragments arranged by size:
2661 2037 455
BsaHI Gr'CG_yC
Cuts at: 419 704 1145 2584 2832 3534 5030 419
Size: 285 441 1439 248 702 1496 542
Fragments arranged by size:
1496 1439 702 542 441 285 248
BsaWI w'CCGG_w
Cuts at: 983 2342 2863 3541 3815 3962 4373 983
Size: 1359 521 678 274 147 411 1763
Fragments arranged by size:
1763 1359 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 5123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 291 406 475 769 844 1189 1326 1329 1861
Size: 115 69 294 75 345 137 3 532
Cuts at: 1861 291
Size: 3583
Fragments arranged by size:
3583 532 345 294 137 115 75 69
3
BseYI C'CCAG_C
Cuts at: 707 723 1086 1116 1370 1883 2578 3336 3913
Size: 16 363 30 254 513 695 758 577
Cuts at: 3913 4409 707
Size: 496 1451
Fragments arranged by size:
1451 758 695 577 513 496 363 254
30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2053
Size: 5153
BsiEI CG_ry'CG
Cuts at: 104 794 1566 1632 2695 2741 3949 104
Size: 690 772 66 1063 46 1208 1308
Fragments arranged by size:
1308 1208 1063 772 690 66 46
BsiHKAI G_wGCw'C
Cuts at: 656 1526 1961 2066 2081 2167 2945 3135 3927
Size: 870 435 105 15 86 778 190 792
Cuts at: 3927 656
Size: 1882
Fragments arranged by size:
1882 870 792 778 435 190 105 86
15
BsmI GAATG_Cn'
Cuts at: 839 839
Size: 5153
BsmBI CGTCTCn'nnnn_
Cuts at: 1786 1786
Size: 5153
Bsp1286I G_dGCh'C
Cuts at: 656 1207 1304 1526 1646 1714 1961 1989 2066
Size: 551 97 222 120 68 247 28 77
Cuts at: 2066 2081 2167 2778 2871 2945 3135 3197 3927
Size: 15 86 611 93 74 190 62 730
Cuts at: 3927 656
Size: 1882
Fragments arranged by size:
1882 730 611 551 247 222 190 120
97 93 86 77 74 68 62 28
15
BspEI T'CCGG_A
Cuts at: 4373 4373
Size: 5153
BspHI T'CATG_A
Cuts at: 4329 4329
Size: 5153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1946 2203 2417 2719 3100 1946
Size: 257 214 302 381 3999
Fragments arranged by size:
3999 381 302 257 214
BsrBI CCG'CTC
Cuts at: 50 1755 1820 3445 3499 4983 5148 50
Size: 1705 65 1625 54 1484 165 55
Fragments arranged by size:
1705 1625 1484 165 65 55 54
BsrDI GCAATG_nn'
Cuts at: 3065 3065
Size: 5153
BsrFI r'CCGG_y
Cuts at: 789 908 1561 3151 3332 3541 789
Size: 119 653 1590 181 209 2401
Fragments arranged by size:
2401 1590 653 209 181 119
BsrGI T'GTAC_A
Cuts at: 890 890
Size: 5153
BssSI C'ACGA_G
Cuts at: 3424 3782 3424
Size: 358 4795
BstAPI GCAn_nnn'nTGC
Cuts at: 1317 2530 1317
Size: 1213 3940
BstBI TT'CG_AA
Cuts at: 3514 3514
Size: 5153
BstYI r'GATC_y
Cuts at: 1 600 2178 2645 2668 3003 3249 4261 4347
Size: 599 1578 467 23 335 246 1012 86
Cuts at: 4347 4359 4705 1
Size: 12 346 449
Fragments arranged by size:
1578 1012 599 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 458 458
Size: 5153
BtgI C'CryG_G
Cuts at: 665 2072 665
Size: 1407 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1473 3103 3284 3447 1473
Size: 1630 181 163 3179
Fragments arranged by size:
3179 1630 181 163
BtsI GCAGTG_nn'
Cuts at: 2396 2396
Size: 5153
DraI TTT'AAA
Cuts at: 2152 2152
Size: 5153
DrdI GACnn_nn'nnGTC
Cuts at: 1144 2859 3717 1144
Size: 1715 858 2580
Fragments arranged by size:
2580 1715 858
EaeI y'GGCC_r
Cuts at: 164 473 527 787 791 881 1400 1563 1583
Size: 309 54 260 4 90 519 163 20
Cuts at: 1583 1629 2021 2738 2912 3303 3330 4675 164
Size: 46 392 717 174 391 27 1345 642
Fragments arranged by size:
1345 717 642 519 392 391 309 260
174 163 90 54 46 27 20 4
EagI C'GGCC_G
Cuts at: 791 1563 1629 2738 791
Size: 772 66 1109 3206
Fragments arranged by size:
3206 1109 772 66
EarI CTCTTCn'nnn_
Cuts at: 250 910 3176 3386 250
Size: 660 2266 210 2017
Fragments arranged by size:
2266 2017 660 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1660 3361 3671 3817 4963 1660
Size: 1701 310 146 1146 1850
Fragments arranged by size:
1850 1701 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2064 2165 2064
Size: 101 5052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 712 934 2978 3410 4157 4425 231
Size: 481 222 2044 432 747 268 959
Fragments arranged by size:
2044 959 747 481 432 268 222
EcoNI CCTnn'n_nnAGG
Cuts at: 412 1171 412
Size: 759 4394
EcoO109I rG'GnC_Cy
Cuts at: 1711 2292 1711
Size: 581 4572
EcoRI G'AATT_C
Cuts at: 2157 2157
Size: 5153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 196 957 3172 196
Size: 761 2215 2177
Fragments arranged by size:
2215 2177 761
FseI GG_CCGG'CC
Cuts at: 793 1565 793
Size: 772 4381
FspI TGC'GCA
Cuts at: 1324 1657 2934 1324
Size: 333 1277 3543
Fragments arranged by size:
3543 1277 333
HaeII r_GCGC'y
Cuts at: 707 1011 2587 2835 3857 4836 707
Size: 304 1576 248 1022 979 1024
Fragments arranged by size:
1576 1024 1022 979 304 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 1162 1194 1405 1437 2995 3025 3027
Size: 32 1003 32 211 32 1558 30 2
Cuts at: 3027 3057 127
Size: 30 2223
Fragments arranged by size:
2223 1558 1003 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1110 2192 1110
Size: 1082 4071
KasI G'GCGC_C
Cuts at: 703 2583 2831 703
Size: 1880 248 3025
Fragments arranged by size:
3025 1880 248
KpnI G_GTAC'C
Cuts at: 2173 2173
Size: 5153
MluI A'CGCG_T
Cuts at: 4586 4586
Size: 5153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 3824 4008 1205
Size: 2619 184 2350
Fragments arranged by size:
2619 2350 184
MscI TGG'CCA
Cuts at: 529 1402 1585 2023 2914 529
Size: 873 183 438 891 2768
Fragments arranged by size:
2768 891 873 438 183
MslI CAynn'nnrTG
Cuts at: 358 659 1200 1448 1529 1651 1705 1723 3142
Size: 301 541 248 81 122 54 18 1419
Cuts at: 3142 3269 358
Size: 127 2242
Fragments arranged by size:
2242 1419 541 301 248 127 122 81
54 18
MspA1I CmG'CkG
Cuts at: 451 1116 2264 2578 2938 3951 4196 451
Size: 665 1148 314 360 1013 245 1408
Fragments arranged by size:
1408 1148 1013 665 360 314 245
NaeI GCC'GGC
Cuts at: 791 910 1563 3334 791
Size: 119 653 1771 2610
Fragments arranged by size:
2610 1771 653 119
NarI GG'CG_CC
Cuts at: 704 2584 2832 704
Size: 1880 248 3025
Fragments arranged by size:
3025 1880 248
NcoI C'CATG_G
Cuts at: 665 2072 665
Size: 1407 3746
NgoMIV G'CCGG_C
Cuts at: 789 908 1561 3332 789
Size: 119 653 1771 2610
Fragments arranged by size:
2610 1771 653 119
NspI r_CATG'y
Cuts at: 447 2206 2706 3613 447
Size: 1759 500 907 1987
Fragments arranged by size:
1987 1759 907 500
PciI A'CATG_T
Cuts at: 3609 3609
Size: 5153
PflMI CCAn_nnn'nTGG
Cuts at: 1592 1592
Size: 5153
PfoI T'CCnGG_A
Cuts at: 1748 1748
Size: 5153
PmeI GTTT'AAAC
Cuts at: 2152 2152
Size: 5153
PmlI CAC'GTG
Cuts at: 132 1074 132
Size: 942 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 4292
Size: 32 5121
PspOMI G'GGCC_C
Cuts at: 1710 1710
Size: 5153
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 631 663 2029 2061 631
Size: 32 1366 32 3723
Fragments arranged by size:
3723 1366 32 32
PstI C_TGCA'G
Cuts at: 390 587 972 1412 1472 2200 2885 390
Size: 197 385 440 60 728 685 2658
Fragments arranged by size:
2658 728 685 440 385 197 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5153
PvuII CAG'CTG
Cuts at: 1116 2578 2938 1116
Size: 1462 360 3331
Fragments arranged by size:
3331 1462 360
RsrII CG'GwC_CG
Cuts at: 3348 3348
Size: 5153
SacI G_AGCT'C
Cuts at: 2066 2167 2066
Size: 101 5052
SalI G'TCGA_C
Cuts at: 2190 2190
Size: 5153
SapI GCTCTTCn'nnn_
Cuts at: 910 3176 3386 910
Size: 2266 210 2677
Fragments arranged by size:
2677 2266 210
SbfI CC_TGCA'GG
Cuts at: 1412 1472 2200 1412
Size: 60 728 4365
Fragments arranged by size:
4365 728 60
ScaI AGT'ACT
Cuts at: 4670 4670
Size: 5153
SfcI C'TryA_G
Cuts at: 33 386 583 968 1408 1468 2196 2881 3874
Size: 353 197 385 440 60 728 685 993
Cuts at: 3874 4065 33
Size: 191 1121
Fragments arranged by size:
1121 993 728 685 440 385 353 197
191 60
SfoI GGC'GCC
Cuts at: 705 2585 2833 705
Size: 1880 248 3025
Fragments arranged by size:
3025 1880 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5153
SmaI CCC'GGG
Cuts at: 1906 2175 1906
Size: 269 4884
SmlI C'TyrA_G
Cuts at: 2308 3223 3715 3977 2308
Size: 915 492 262 3484
Fragments arranged by size:
3484 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5153
SphI G_CATG'C
Cuts at: 2206 2706 2206
Size: 500 4653
StyI C'CwwG_G
Cuts at: 413 665 884 1502 1556 1604 2072 2117 2287
Size: 252 219 618 54 48 468 45 170
Cuts at: 2287 2641 413
Size: 354 2925
Fragments arranged by size:
2925 618 468 354 252 219 170 54
48 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1120 1208 1362 1897 1967 2736 2869 3328 3537
Size: 88 154 535 70 769 133 459 209
Cuts at: 3537 1120
Size: 2736
Fragments arranged by size:
2736 769 535 459 209 154 133 88
70
TatI w'GTAC_w
Cuts at: 890 1802 4668 890
Size: 912 2866 1375
Fragments arranged by size:
2866 1375 912
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4670 4702 4670
Size: 32 5121
Tth111I GACn'n_nGTC
Cuts at: 1147 2950 1147
Size: 1803 3350
XbaI T'CTAG_A
Cuts at: 2184 2184
Size: 5153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 891 2648 114
Size: 777 1757 2619
Fragments arranged by size:
2619 1757 777
XmaI C'CCGG_G
Cuts at: 1904 2173 1904
Size: 269 4884
XmnI GAAnn'nnTTC
Cuts at: 958 2041 958
Size: 1083 4070
ZraI GAC'GTC
Cuts at: 1146 1146
Size: 5153
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BbsI BbvCI
BfrBI BglI BmtI BsiWI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI