Restriction map for
pF1KB5554
(Circular) (Six-base) MAPSORT of: pF1KB5554.seq Check: 8201 from: 1 to: 4007
pF1KB5554 4007 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 143 1045 143
Size: 902 3105
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 568 1832 2264 3011 3279 325
Size: 243 1264 432 747 268 1053
Fragments arranged by size:
1264 1053 747 432 268 243
AflIII A'CryG_T
Cuts at: 729 1359 2463 3440 729
Size: 630 1104 977 1296
Fragments arranged by size:
1296 1104 977 630
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AhdI GACnn_n'nnGTC
Cuts at: 841 879 841
Size: 38 3969
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 332 2777 332
Size: 2445 1562
ApoI r'AATT_y
Cuts at: 413 1011 3360 3616 413
Size: 598 2349 256 804
Fragments arranged by size:
2349 804 598 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 954 1023 1437 1685 1720 954
Size: 69 414 248 35 3241
Fragments arranged by size:
3241 414 248 69 35
BanII G_rGCy'C
Cuts at: 266 619 1021 2051 266
Size: 353 402 1030 2222
Fragments arranged by size:
2222 1030 402 353
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BbsI GAAGACnn'nnnn_
Cuts at: 137 793 137
Size: 656 3351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 458 690 724 424
Size: 34 232 34 3707
Fragments arranged by size:
3707 232 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 266 1905 2666 4007 266
Size: 1639 761 1341 266
Fragments arranged by size:
1639 1341 761 266
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BfrBI ATG'CAT
Cuts at: 711 711
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 336 1632 1725 2781 336
Size: 1296 93 1056 1562
Fragments arranged by size:
1562 1296 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1219 1300 1635 3779 1219
Size: 81 335 2144 1447
Fragments arranged by size:
2144 1447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 527
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 307 1360 307
Size: 1053 2954
BsaBI GATnn'nnATC
Cuts at: 6 236 1521 3558 6
Size: 230 1285 2037 455
Fragments arranged by size:
2037 1285 455 230
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1432 2190 2767 3263 1432
Size: 758 577 496 2176
Fragments arranged by size:
2176 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 626
Size: 4007
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 266 336 619 641 1021 1799 1989 2781 266
Size: 70 283 22 380 778 190 792 1492
Fragments arranged by size:
1492 792 778 380 283 190 70 22
Bsp1286I G_dGCh'C
Cuts at: 266 336 619 641 1021 1632 1725 1799 1989
Size: 70 283 22 380 611 93 74 190
Cuts at: 1989 2051 2781 266
Size: 62 730 1492
Fragments arranged by size:
1492 730 611 380 283 190 93 74
70 62 22
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1057 1271 1573 1954 1057
Size: 214 302 381 3110
Fragments arranged by size:
3110 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 184 636 1384 184
Size: 452 748 2807
Fragments arranged by size:
2807 748 452
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstYI r'GATC_y
Cuts at: 1 1032 1499 1522 1857 2103 3115 3201 3213
Size: 1031 467 23 335 246 1012 86 12
Cuts at: 3213 3559 1
Size: 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 995 995
Size: 4007
BtgI C'CryG_G
Cuts at: 320 320
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
DraI TTT'AAA
Cuts at: 290 747 1006 290
Size: 457 259 3291
Fragments arranged by size:
3291 457 259
DraIII CAC_nnn'GTG
Cuts at: 636 636
Size: 4007
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 317 1592 1766 2157 2184 3529 317
Size: 1275 174 391 27 1345 795
Fragments arranged by size:
1345 1275 795 391 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 264 617 1019 264
Size: 353 402 3252
Fragments arranged by size:
3252 402 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 527 568 1832 2264 3011 3279 325
Size: 202 41 1264 432 747 268 1053
Fragments arranged by size:
1264 1053 747 432 268 202 41
EcoO109I rG'GnC_Cy
Cuts at: 686 1146 686
Size: 460 3547
EcoRI G'AATT_C
Cuts at: 413 1011 413
Size: 598 3409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 1441 1689 2711 3690 1441
Size: 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 827 859 865 897 1849 1879 1881 1911 827
Size: 32 6 32 952 30 2 30 2923
Fragments arranged by size:
2923 952 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 884 1046 884
Size: 162 3845
HindIII A'AGCT_T
Cuts at: 209 209
Size: 4007
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 390 2678 2862 390
Size: 2288 184 1535
Fragments arranged by size:
2288 1535 184
MscI TGG'CCA
Cuts at: 1768 1768
Size: 4007
MslI CAynn'nnrTG
Cuts at: 1996 2123 1996
Size: 127 3880
MspA1I CmG'CkG
Cuts at: 1118 1432 1792 2805 3050 1118
Size: 314 360 1013 245 2075
Fragments arranged by size:
2075 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NsiI A_TGCA'T
Cuts at: 713 713
Size: 4007
NspI r_CATG'y
Cuts at: 733 1060 1560 2467 733
Size: 327 500 907 2273
Fragments arranged by size:
2273 907 500 327
PciI A'CATG_T
Cuts at: 729 2463 729
Size: 1734 2273
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PstI C_TGCA'G
Cuts at: 789 1054 1739 789
Size: 265 685 3057
Fragments arranged by size:
3057 685 265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 266 619 1021 266
Size: 353 402 3252
Fragments arranged by size:
3252 402 353
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 581 3524 581
Size: 2943 1064
SfcI C'TryA_G
Cuts at: 33 785 896 1050 1735 2728 2919 33
Size: 752 111 154 685 993 191 1121
Fragments arranged by size:
1121 993 752 685 191 154 111
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
StyI C'CwwG_G
Cuts at: 1141 1495 1141
Size: 354 3653
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1590 1723 2182 2391 1590
Size: 133 459 209 3206
Fragments arranged by size:
3206 459 209 133
TatI w'GTAC_w
Cuts at: 579 3522 579
Size: 2943 1064
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 3975
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI