Restriction map for
pF1KB5555
(Circular) (Six-base) MAPSORT of: pF1KB5555.seq Check: 5055 from: 1 to: 4469
pF1KB5555 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2294 2726 3079 3741 2294
Size: 432 353 662 3022
Fragments arranged by size:
3022 662 432 353
AflIII A'CryG_T
Cuts at: 1821 3621 3902 1821
Size: 1800 281 2388
Fragments arranged by size:
2388 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 153 917 153
Size: 764 3705
AleI CACnn'nnGTG
Cuts at: 661 1435 661
Size: 774 3695
AlwNI CAG_nnn'CTG
Cuts at: 903 1067 1212 1774 3212 903
Size: 164 145 562 1438 2160
Fragments arranged by size:
2160 1438 562 164 145
ApaI G_GGCC'C
Cuts at: 910 1122 910
Size: 212 4257
ApaLI G'TGCA_C
Cuts at: 1159 3307 1159
Size: 2148 2321
ApoI r'AATT_y
Cuts at: 810 1183 1473 3822 4078 810
Size: 373 290 2349 256 1201
Fragments arranged by size:
2349 1201 373 290 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 216 1489 216
Size: 1273 3196
BamHI G'GATC_C
Cuts at: 331 1494 331
Size: 1163 3306
BanI G'GyrC_C
Cuts at: 1485 1899 2147 2182 1485
Size: 414 248 35 3772
Fragments arranged by size:
3772 414 248 35
BanII G_rGCy'C
Cuts at: 910 1122 1483 2513 910
Size: 212 361 1030 2866
Fragments arranged by size:
2866 1030 361 212
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 767 767
Size: 4469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 227 193
Size: 34 4435
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3423 4469 2367
Size: 1056 1046 2367
Fragments arranged by size:
2367 1056 1046
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglII A'GATC_T
Cuts at: 1984 4021 1984
Size: 2037 2432
BlpI GC'TnA_GC
Cuts at: 1111 1581 1111
Size: 470 3999
Bme1580I G_kGCm'C
Cuts at: 849 910 1122 1163 2094 2187 3311 849
Size: 61 212 41 931 93 1124 2007
Fragments arranged by size:
2007 1124 931 212 93 61 41
BmrI ACTGGGnnnn_n'
Cuts at: 1681 1762 2097 4241 1681
Size: 81 335 2144 1909
Fragments arranged by size:
2144 1909 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 996 2836 996
Size: 1840 2629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 991 1329 553
Size: 438 338 3693
Fragments arranged by size:
3693 438 338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3238 3536 1645
Size: 879 714 298 2578
Fragments arranged by size:
2578 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4173 4173
Size: 4469
BsaAI yAC'GTr
Cuts at: 1437 1822 1437
Size: 385 4084
BsaBI GATnn'nnATC
Cuts at: 6 565 893 1983 4020 6
Size: 559 328 1090 2037 455
Fragments arranged by size:
2037 1090 559 455 328
BsaHI Gr'CG_yC
Cuts at: 1900 2148 2850 4346 1900
Size: 248 702 1496 2023
Fragments arranged by size:
2023 1496 702 248
BsaWI w'CCGG_w
Cuts at: 345 1658 2179 2857 3268 3415 3689 345
Size: 1313 521 678 411 147 274 1125
Fragments arranged by size:
1313 1125 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 1363 1393 118
Size: 30 1215 30 3194
Fragments arranged by size:
3194 1215 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 437 486 803 437
Size: 49 317 4103
Fragments arranged by size:
4103 317 49
BseYI C'CCAG_C
Cuts at: 881 1067 1894 2652 3317 3725 881
Size: 186 827 758 665 408 1625
Fragments arranged by size:
1625 827 758 665 408 186
BsiEI CG_ry'CG
Cuts at: 104 1229 2011 2057 3287 104
Size: 1125 782 46 1230 1286
Fragments arranged by size:
1286 1230 1125 782 46
BsiHKAI G_wGCw'C
Cuts at: 1163 1483 2261 2451 3311 1163
Size: 320 778 190 860 2321
Fragments arranged by size:
2321 860 778 320 190
BsiWI C'GTAC_G
Cuts at: 173 173
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 849 910 1122 1163 1483 2094 2187 2261 2451
Size: 61 212 41 320 611 93 74 190
Cuts at: 2451 2513 3311 849
Size: 62 798 2007
Fragments arranged by size:
2007 798 611 320 212 190 93 74
62 61 41
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1519 1733 2035 2416 1519
Size: 214 302 381 3572
Fragments arranged by size:
3572 381 302 214
BsrBI CCG'CTC
Cuts at: 50 181 1077 2761 2815 4299 4464 50
Size: 131 896 1684 54 1484 165 55
Fragments arranged by size:
1684 1484 896 165 131 55 54
BsrDI GCAATG_nn'
Cuts at: 532 1199 2381 532
Size: 667 1182 2620
Fragments arranged by size:
2620 1182 667
BsrFI r'CCGG_y
Cuts at: 2467 2648 2467
Size: 181 4288
BsrGI T'GTAC_A
Cuts at: 1241 1241
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3448 2740
Size: 708 3761
BstAPI GCAn_nnn'nTGC
Cuts at: 1846 1846
Size: 4469
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstXI CCAn_nnnn'nTGG
Cuts at: 328 328
Size: 4469
BstYI r'GATC_y
Cuts at: 1 331 888 899 947 1494 1961 1984 2319
Size: 330 557 11 48 547 467 23 335
Cuts at: 2319 2565 2871 2883 2969 4021 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 557 547 467 449 335 330 306
246 86 48 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 688 688
Size: 4469
BtgI C'CryG_G
Cuts at: 107 1194 1385 107
Size: 1087 191 3191
Fragments arranged by size:
3191 1087 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 700 1712 700
Size: 1012 3457
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 503 503
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2175 3519 1233
Size: 942 1344 2183
Fragments arranged by size:
2183 1344 942
EaeI y'GGCC_r
Cuts at: 1058 2054 2228 2619 2646 3991 1058
Size: 996 174 391 27 1345 1536
Fragments arranged by size:
1536 1345 996 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 780 2492 2702 780
Size: 1712 210 2547
Fragments arranged by size:
2547 1712 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 401 2677 3419 3565 4279 401
Size: 2276 742 146 714 591
Fragments arranged by size:
2276 742 714 591 146
EcoICRI GAG'CTC
Cuts at: 1481 1481
Size: 4469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 991 1329 2294 2726 3079 3741 553
Size: 438 338 965 432 353 662 1281
Fragments arranged by size:
1281 965 662 438 432 353 338
EcoO109I rG'GnC_Cy
Cuts at: 1119 1608 1119
Size: 489 3980
EcoRI G'AATT_C
Cuts at: 810 1473 810
Size: 663 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 645 809 1193 1320 2488 645
Size: 164 384 127 1168 2626
Fragments arranged by size:
2626 1168 384 164 127
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 992 1068 1903 2151 3381 4152 992
Size: 76 835 248 1230 771 1309
Fragments arranged by size:
1309 1230 835 771 248 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 139 150 171 467 499 724 737 756
Size: 21 11 21 296 32 225 13 19
Cuts at: 756 769 903 935 2311 2341 2343 2373 118
Size: 13 134 32 1376 30 2 30 2214
Fragments arranged by size:
2214 1376 296 225 134 32 32 30
30 21 21 19 13 13 11 2
HincII GTy'rAC
Cuts at: 300 1508 300
Size: 1208 3261
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 1007 1249 3228 3412 422
Size: 585 242 1979 184 1479
Fragments arranged by size:
1979 1479 585 242 184
MscI TGG'CCA
Cuts at: 1060 2230 1060
Size: 1170 3299
MslI CAynn'nnrTG
Cuts at: 326 661 1435 2458 2585 326
Size: 335 774 1023 127 2210
Fragments arranged by size:
2210 1023 774 335 127
MspA1I CmG'CkG
Cuts at: 954 1001 1580 1894 2254 3038 3283 954
Size: 47 579 314 360 784 245 2140
Fragments arranged by size:
2140 784 579 360 314 245 47
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 107 107
Size: 4469
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 520 1272 1522 2022 3625 520
Size: 752 250 500 1603 1364
Fragments arranged by size:
1603 1364 752 500 250
PciI A'CATG_T
Cuts at: 3621 3621
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 503 591 1067 503
Size: 88 476 3905
Fragments arranged by size:
3905 476 88
PfoI T'CCnGG_A
Cuts at: 968 968
Size: 4469
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PmlI CAC'GTG
Cuts at: 1437 1437
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 4437
PspOMI G'GGCC_C
Cuts at: 906 1118 906
Size: 212 4257
PstI C_TGCA'G
Cuts at: 358 1217 1516 2201 358
Size: 859 299 685 2626
Fragments arranged by size:
2626 859 685 299
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 954 1001 1894 2254 954
Size: 47 893 360 3169
Fragments arranged by size:
3169 893 360 47
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1483 1483
Size: 4469
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 2492 2702 2492
Size: 210 4259
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 354 1213 1512 2197 3165 3356 33
Size: 321 859 299 685 968 191 1146
Fragments arranged by size:
1146 968 859 685 321 299 191
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 1624 2539 3253 3515 1624
Size: 915 714 262 2578
Fragments arranged by size:
2578 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
StyI C'CwwG_G
Cuts at: 107 197 837 1603 1957 107
Size: 90 640 766 354 2619
Fragments arranged by size:
2619 766 640 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1243 1447 2052 2185 2644 2853 1243
Size: 204 605 133 459 209 2859
Fragments arranged by size:
2859 605 459 209 204 133
TatI w'GTAC_w
Cuts at: 551 1241 3984 551
Size: 690 2743 1036
Fragments arranged by size:
2743 1036 690
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 4437
Tth111I GACn'n_nGTC
Cuts at: 778 1230 2266 778
Size: 452 1036 2981
Fragments arranged by size:
2981 1036 452
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 272 592 1964 272
Size: 320 1372 2777
Fragments arranged by size:
2777 1372 320
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsmI BsmBI BspEI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI