Restriction map for
pF1KB5556
(Circular) (Six-base) MAPSORT of: pF1KB5556.seq Check: 9411 from: 1 to: 4106
pF1KB5556 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 746 746
Size: 4106
AccI GT'mk_AC
Cuts at: 614 1144 614
Size: 530 3576
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 567 802 1931 2363 2716 3378 274
Size: 293 235 1129 432 353 662 1002
Fragments arranged by size:
1129 1002 662 432 353 293 235
AflIII A'CryG_T
Cuts at: 1458 3258 3539 1458
Size: 1800 281 2025
Fragments arranged by size:
2025 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 849 849
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 399 780 1411 2849 399
Size: 381 631 1438 1656
Fragments arranged by size:
1656 1438 631 381
ApaLI G'TGCA_C
Cuts at: 2944 2944
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 328 805 1126 328
Size: 477 321 3308
Fragments arranged by size:
3308 477 321
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 666 1064 1122 1536 1784 1819 666
Size: 398 58 414 248 35 2953
Fragments arranged by size:
2953 414 398 248 58 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3076
BbeI G_GCGC'C
Cuts at: 670 1540 1788 670
Size: 870 248 2988
Fragments arranged by size:
2988 870 248
BbvCI CC'TCA_GC
Cuts at: 625 625
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 2004 3060 4106 2004
Size: 1056 1046 2004
Fragments arranged by size:
2004 1056 1046
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglI GCCn_nnn'nGGC
Cuts at: 556 691 556
Size: 135 3971
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 649 1218 649
Size: 569 3537
Bme1580I G_kGCm'C
Cuts at: 368 406 806 1731 1824 2948 368
Size: 38 400 925 93 1124 1526
Fragments arranged by size:
1526 1124 925 400 93 38
BmgBI CAC'GTC
Cuts at: 303 500 303
Size: 197 3909
BmrI ACTGGGnnnn_n'
Cuts at: 235 387 1318 1399 1734 3878 235
Size: 152 931 81 335 2144 463
Fragments arranged by size:
2144 931 463 335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 949 967 355
Size: 594 18 3494
Fragments arranged by size:
3494 594 18
Bpu10I CC'TnA_GC
Cuts at: 625 1009 625
Size: 384 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1282 2161 2875 3173 298
Size: 984 879 714 298 1231
Fragments arranged by size:
1231 984 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 322 667 991 1537 1785 2487 3983 322
Size: 345 324 546 248 702 1496 445
Fragments arranged by size:
1496 702 546 445 345 324 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2905 3052 3326 1295
Size: 521 678 411 147 274 2075
Fragments arranged by size:
2075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 976 1006 976
Size: 30 4076
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 802 277
Size: 525 3581
BseYI C'CCAG_C
Cuts at: 490 710 743 1531 2289 2954 3362 490
Size: 220 33 788 758 665 408 1234
Fragments arranged by size:
1234 788 758 665 408 220 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 580 778 454
Size: 126 198 3782
Fragments arranged by size:
3782 198 126
BsiEI CG_ry'CG
Cuts at: 104 686 1648 1694 2924 104
Size: 582 962 46 1230 1286
Fragments arranged by size:
1286 1230 962 582 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1898 2088 2948 1120
Size: 778 190 860 2278
Fragments arranged by size:
2278 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 368 406 806 1120 1731 1824 1898 2088 2150
Size: 38 400 314 611 93 74 190 62
Cuts at: 2150 2948 368
Size: 798 1526
Fragments arranged by size:
1526 798 611 400 314 190 93 74
62 38
BspHI T'CATG_A
Cuts at: 1043 2538 1043
Size: 1495 2611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 746 1156 1370 1672 2053 746
Size: 410 214 302 381 2799
Fragments arranged by size:
2799 410 381 302 214
BsrBI CCG'CTC
Cuts at: 50 176 861 2398 2452 3936 4101 50
Size: 126 685 1537 54 1484 165 55
Fragments arranged by size:
1537 1484 685 165 126 55 54
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 513 609 685 2104 2285 513
Size: 96 76 1419 181 2334
Fragments arranged by size:
2334 1419 181 96 76
BssHII G'CGCG_C
Cuts at: 453 644 453
Size: 191 3915
BssSI C'ACGA_G
Cuts at: 2377 3085 2377
Size: 708 3398
BstAPI GCAn_nnn'nTGC
Cuts at: 738 1089 1483 738
Size: 351 394 3361
Fragments arranged by size:
3361 394 351
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 846 846
Size: 4106
BstYI r'GATC_y
Cuts at: 1 203 1131 1598 1621 1956 2202 2508 2520
Size: 202 928 467 23 335 246 306 12
Cuts at: 2520 2606 3658 1
Size: 86 1052 449
Fragments arranged by size:
1052 928 467 449 335 306 246 202
86 23 12
BtgI C'CryG_G
Cuts at: 542 766 995 542
Size: 224 229 3653
Fragments arranged by size:
3653 229 224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 882 2056 2237 2400 882
Size: 1174 181 163 2588
Fragments arranged by size:
2588 1174 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 3156 1812
Size: 1344 2762
EaeI y'GGCC_r
Cuts at: 233 461 683 998 1691 1865 2256 2283 3628
Size: 228 222 315 693 174 391 27 1345
Cuts at: 3628 233
Size: 711
Fragments arranged by size:
1345 711 693 391 315 228 222 174
27
EagI C'GGCC_G
Cuts at: 683 1691 683
Size: 1008 3098
EarI CTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 886 2314 3056 3202 3916 886
Size: 1428 742 146 714 1076
Fragments arranged by size:
1428 1076 742 714 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 355 567 802 949 967 1931 2363 2716
Size: 81 212 235 147 18 964 432 353
Cuts at: 2716 3378 274
Size: 662 1002
Fragments arranged by size:
1002 964 662 432 353 235 212 147
81 18
EcoNI CCTnn'n_nnAGG
Cuts at: 784 784
Size: 4106
EcoO109I rG'GnC_Cy
Cuts at: 777 1245 777
Size: 468 3638
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FseI GG_CCGG'CC
Cuts at: 689 689
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 342 590 670 1540 1788 3018 3789 342
Size: 248 80 870 248 1230 771 659
Fragments arranged by size:
1230 870 771 659 248 248 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 835 867 976 1008 1948 1978 1980 2010 835
Size: 32 109 32 940 30 2 30 2931
Fragments arranged by size:
2931 940 109 32 32 30 30 2
HincII GTy'rAC
Cuts at: 519 1056 1145 519
Size: 537 89 3480
Fragments arranged by size:
3480 537 89
HpaI GTT'AAC
Cuts at: 1056 1056
Size: 4106
KasI G'GCGC_C
Cuts at: 666 1536 1784 666
Size: 870 248 2988
Fragments arranged by size:
2988 870 248
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2865 3049 2865
Size: 184 3922
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 844 2095 2222 844
Size: 1251 127 2728
Fragments arranged by size:
2728 1251 127
MspA1I CmG'CkG
Cuts at: 544 569 575 656 768 1217 1531 1891 2675
Size: 25 6 81 112 449 314 360 784
Cuts at: 2675 2920 544
Size: 245 1730
Fragments arranged by size:
1730 784 449 360 314 245 112 81
25 6
NaeI GCC'GGC
Cuts at: 687 2287 687
Size: 1600 2506
NarI GG'CG_CC
Cuts at: 667 1537 1785 667
Size: 870 248 2988
Fragments arranged by size:
2988 870 248
NgoMIV G'CCGG_C
Cuts at: 685 2285 685
Size: 1600 2506
NspI r_CATG'y
Cuts at: 1008 1159 1659 3262 1008
Size: 151 500 1603 1852
Fragments arranged by size:
1852 1603 500 151
PciI A'CATG_T
Cuts at: 3258 3258
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 4074
PstI C_TGCA'G
Cuts at: 776 1153 1838 776
Size: 377 685 3044
Fragments arranged by size:
3044 685 377
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SacII CC_GC'GG
Cuts at: 545 769 545
Size: 224 3882
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 189 772 1149 1834 2802 2993 33
Size: 156 583 377 685 968 191 1146
Fragments arranged by size:
1146 968 685 583 377 191 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 691 691
Size: 4106
SfoI GGC'GCC
Cuts at: 668 1538 1786 668
Size: 870 248 2988
Fragments arranged by size:
2988 870 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 313 1261 2176 2890 3152 313
Size: 948 915 714 262 1267
Fragments arranged by size:
1267 948 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 1240 1594 1240
Size: 354 3752
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1052 1689 1822 2281 2490 406
Size: 646 637 133 459 209 2022
Fragments arranged by size:
2022 646 637 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1057 1601 1057
Size: 544 3562
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI
BsmI BsmBI BspEI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FspAI HindIII MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI