Restriction map for
pF1KB5558
(Circular) (Six-base) MAPSORT of: pF1KB5558.seq Check: 566 from: 1 to: 4832
pF1KB5558 4832 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1546 1546
Size: 4832
AccI GT'mk_AC
Cuts at: 239 1870 239
Size: 1631 3201
Acc65I G'GTAC_C
Cuts at: 1848 1848
Size: 4832
AclI AA'CG_TT
Cuts at: 4672 4672
Size: 4832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 953 976 1339 2657 3089 3836 4104 623
Size: 330 23 363 1318 432 747 268 1351
Fragments arranged by size:
1351 1318 747 432 363 330 268 23
AflIII A'CryG_T
Cuts at: 2184 3288 4265 2184
Size: 1104 977 2751
Fragments arranged by size:
2751 1104 977
AgeI A'CCGG_T
Cuts at: 144 3220 144
Size: 3076 1756
AlwNI CAG_nnn'CTG
Cuts at: 2137 3704 2137
Size: 1567 3265
ApaLI G'TGCA_C
Cuts at: 3602 3602
Size: 4832
ApoI r'AATT_y
Cuts at: 199 820 857 1526 1751 1836 4185 4441 199
Size: 621 37 669 225 85 2349 256 590
Fragments arranged by size:
2349 669 621 590 256 225 85 37
AseI AT'TA_AT
Cuts at: 20 794 20
Size: 774 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
AvaI C'yCGr_G
Cuts at: 176 215 1852 176
Size: 39 1637 3156
Fragments arranged by size:
3156 1637 39
BamHI G'GATC_C
Cuts at: 1857 1857
Size: 4832
BanI G'GyrC_C
Cuts at: 1848 2262 2510 2545 1848
Size: 414 248 35 4135
Fragments arranged by size:
4135 414 248 35
BanII G_rGCy'C
Cuts at: 1846 2876 1846
Size: 1030 3802
BbeI G_GCGC'C
Cuts at: 2266 2514 2266
Size: 248 4584
BbsI GAAGACnn'nnnn_
Cuts at: 982 1118 982
Size: 136 4696
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4798
BciVI GTATCCnnnnn_n'
Cuts at: 877 2730 3491 4832 877
Size: 1853 761 1341 877
Fragments arranged by size:
1853 1341 877 761
BclI T'GATC_A
Cuts at: 2352 2352
Size: 4832
BfrBI ATG'CAT
Cuts at: 1448 1448
Size: 4832
BglII A'GATC_T
Cuts at: 2347 4384 2347
Size: 2037 2795
BlpI GC'TnA_GC
Cuts at: 1944 1944
Size: 4832
Bme1580I G_kGCm'C
Cuts at: 1437 2457 2550 3606 1437
Size: 1020 93 1056 2663
Fragments arranged by size:
2663 1056 1020 93
BmrI ACTGGGnnnn_n'
Cuts at: 2044 2125 2460 4604 2044
Size: 81 335 2144 2272
Fragments arranged by size:
2272 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 4832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 998 1457 1649 998
Size: 459 192 4181
Fragments arranged by size:
4181 459 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2008 2887 3379 3677 2008
Size: 879 492 298 3163
Fragments arranged by size:
3163 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 135 4536 135
Size: 4401 431
BsaAI yAC'GTr
Cuts at: 2185 2185
Size: 4832
BsaBI GATnn'nnATC
Cuts at: 6 2346 4383 6
Size: 2340 2037 455
Fragments arranged by size:
2340 2037 455
BsaHI Gr'CG_yC
Cuts at: 2263 2511 3213 4709 2263
Size: 248 702 1496 2386
Fragments arranged by size:
2386 1496 702 248
BsaWI w'CCGG_w
Cuts at: 144 2021 2542 3220 3494 3641 4052 144
Size: 1877 521 678 274 147 411 924
Fragments arranged by size:
1877 924 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1298 1328 1622 1652 1298
Size: 30 294 30 4478
Fragments arranged by size:
4478 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 155 155
Size: 4832
BseYI C'CCAG_C
Cuts at: 433 2257 3015 3592 4088 433
Size: 1824 758 577 496 1177
Fragments arranged by size:
1824 1177 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1094 227
Size: 867 3965
BsiEI CG_ry'CG
Cuts at: 104 2374 2420 3628 104
Size: 2270 46 1208 1308
Fragments arranged by size:
2270 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1846 2624 2814 3606 1846
Size: 778 190 792 3072
Fragments arranged by size:
3072 792 778 190
BsmI GAATG_Cn'
Cuts at: 1448 1557 1448
Size: 109 4723
Bsp1286I G_dGCh'C
Cuts at: 1437 1846 2457 2550 2624 2814 2876 3606 1437
Size: 409 611 93 74 190 62 730 2663
Fragments arranged by size:
2663 730 611 409 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4052 4052
Size: 4832
BspHI T'CATG_A
Cuts at: 1125 4008 1125
Size: 2883 1949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 891 1546 1882 2096 2398 2779 891
Size: 655 336 214 302 381 2944
Fragments arranged by size:
2944 655 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 3124 3178 4662 4827 50
Size: 3074 54 1484 165 55
Fragments arranged by size:
3074 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 680 1541 1793 2744 680
Size: 861 252 951 2768
Fragments arranged by size:
2768 951 861 252
BsrFI r'CCGG_y
Cuts at: 144 2830 3011 3220 144
Size: 2686 181 209 1756
Fragments arranged by size:
2686 1756 209 181
BssSI C'ACGA_G
Cuts at: 3103 3461 3103
Size: 358 4474
BstAPI GCAn_nnn'nTGC
Cuts at: 619 2209 619
Size: 1590 3242
BstBI TT'CG_AA
Cuts at: 3193 3193
Size: 4832
BstEII G'GTnAC_C
Cuts at: 116 116
Size: 4832
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 4832
BstYI r'GATC_y
Cuts at: 1 1567 1857 2324 2347 2682 2928 3940 4026
Size: 1566 290 467 23 335 246 1012 86
Cuts at: 4026 4038 4384 1
Size: 12 346 449
Fragments arranged by size:
1566 1012 467 449 346 335 290 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2782 2963 3126 2782
Size: 181 163 4488
Fragments arranged by size:
4488 181 163
BtsI GCAGTG_nn'
Cuts at: 449 1082 2075 449
Size: 633 993 3206
Fragments arranged by size:
3206 993 633
DraI TTT'AAA
Cuts at: 1831 1831
Size: 4832
DrdI GACnn_nn'nnGTC
Cuts at: 2538 3396 2538
Size: 858 3974
EaeI y'GGCC_r
Cuts at: 260 2417 2591 2982 3009 4354 260
Size: 2157 174 391 27 1345 738
Fragments arranged by size:
2157 1345 738 391 174 27
EagI C'GGCC_G
Cuts at: 2417 2417
Size: 4832
EarI CTCTTCn'nnn_
Cuts at: 1082 1757 2855 3065 1082
Size: 675 1098 210 2849
Fragments arranged by size:
2849 1098 675 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3040 3350 3496 4642 3040
Size: 310 146 1146 3230
Fragments arranged by size:
3230 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1844 1844
Size: 4832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 953 976 998 1339 1457 1649 2657 3089
Size: 330 23 22 341 118 192 1008 432
Cuts at: 3089 3836 4104 623
Size: 747 268 1351
Fragments arranged by size:
1351 1008 747 432 341 330 268 192
118 23 22
EcoO109I rG'GnC_Cy
Cuts at: 1770 1971 1770
Size: 201 4631
EcoRI G'AATT_C
Cuts at: 820 1836 820
Size: 1016 3816
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 2851 504
Size: 2347 2485
FspI TGC'GCA
Cuts at: 2613 2613
Size: 4832
HaeII r_GCGC'y
Cuts at: 2266 2514 3536 4515 2266
Size: 248 1022 979 2583
Fragments arranged by size:
2583 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1089 1121 2674 2704 2706 2736 1089
Size: 32 1553 30 2 30 3185
Fragments arranged by size:
3185 1553 32 30 30 2
HincII GTy'rAC
Cuts at: 1724 1871 1724
Size: 147 4685
HpaI GTT'AAC
Cuts at: 1724 1724
Size: 4832
KasI G'GCGC_C
Cuts at: 2262 2510 2262
Size: 248 4584
KpnI G_GTAC'C
Cuts at: 1852 1852
Size: 4832
MfeI C'AATT_G
Cuts at: 356 356
Size: 4832
MluI A'CGCG_T
Cuts at: 4265 4265
Size: 4832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 666 1122 3503 3687 666
Size: 456 2381 184 1811
Fragments arranged by size:
2381 1811 456 184
MscI TGG'CCA
Cuts at: 2593 2593
Size: 4832
MslI CAynn'nnrTG
Cuts at: 2821 2948 2821
Size: 127 4705
MspA1I CmG'CkG
Cuts at: 1943 2257 2617 3630 3875 1943
Size: 314 360 1013 245 2900
Fragments arranged by size:
2900 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3013 3013
Size: 4832
NarI GG'CG_CC
Cuts at: 2263 2511 2263
Size: 248 4584
NgoMIV G'CCGG_C
Cuts at: 3011 3011
Size: 4832
NsiI A_TGCA'T
Cuts at: 1450 1450
Size: 4832
NspI r_CATG'y
Cuts at: 1824 1885 2385 3292 1824
Size: 61 500 907 3364
Fragments arranged by size:
3364 907 500 61
PciI A'CATG_T
Cuts at: 3288 3288
Size: 4832
PflMI CCAn_nnn'nTGG
Cuts at: 1021 1021
Size: 4832
PfoI T'CCnGG_A
Cuts at: 824 824
Size: 4832
PmeI GTTT'AAAC
Cuts at: 1831 1831
Size: 4832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4800
PpuMI rG'GwC_Cy
Cuts at: 1770 1770
Size: 4832
PshAI GACnn'nnGTC
Cuts at: 147 147
Size: 4832
PstI C_TGCA'G
Cuts at: 1879 2564 1879
Size: 685 4147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4832
PvuII CAG'CTG
Cuts at: 2257 2617 2257
Size: 360 4472
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 4832
SacI G_AGCT'C
Cuts at: 1846 1846
Size: 4832
SalI G'TCGA_C
Cuts at: 1869 1869
Size: 4832
SapI GCTCTTCn'nnn_
Cuts at: 2855 3065 2855
Size: 210 4622
SbfI CC_TGCA'GG
Cuts at: 1879 1879
Size: 4832
ScaI AGT'ACT
Cuts at: 4349 4349
Size: 4832
SfcI C'TryA_G
Cuts at: 33 473 1727 1875 2560 3553 3744 33
Size: 440 1254 148 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 440 191 148
SfoI GGC'GCC
Cuts at: 2264 2512 2264
Size: 248 4584
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
SmaI CCC'GGG
Cuts at: 1854 1854
Size: 4832
SmlI C'TyrA_G
Cuts at: 1987 2902 3394 3656 1987
Size: 915 492 262 3163
Fragments arranged by size:
3163 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1252 67
Size: 1185 3647
SphI G_CATG'C
Cuts at: 1885 2385 1885
Size: 500 4332
StyI C'CwwG_G
Cuts at: 479 1457 1966 2320 479
Size: 978 509 354 2991
Fragments arranged by size:
2991 978 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1771 1780 2415 2548 3007 3216 1771
Size: 9 635 133 459 209 3387
Fragments arranged by size:
3387 635 459 209 133 9
TatI w'GTAC_w
Cuts at: 459 958 4347 459
Size: 499 3389 944
Fragments arranged by size:
3389 944 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 275 307 1298 1330 1622 1654 1737 1769 4349
Size: 32 991 32 292 32 83 32 2580
Cuts at: 4349 4381 275
Size: 32 726
Fragments arranged by size:
2580 991 726 292 83 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2629 2629
Size: 4832
XbaI T'CTAG_A
Cuts at: 1863 1863
Size: 4832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2327
Size: 4832
XmaI C'CCGG_G
Cuts at: 1852 1852
Size: 4832
XmnI GAAnn'nnTTC
Cuts at: 1115 1115
Size: 4832
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII NcoI
NdeI NheI NotI NruI PacI PasI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI