Restriction map for
pF1KB5565
(Circular) (Six-base) MAPSORT of: pF1KB5565.seq Check: 2861 from: 1 to: 5324
pF1KB5565 5324 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 968 2362 968
Size: 1394 3930
Acc65I G'GTAC_C
Cuts at: 2340 2340
Size: 5324
AclI AA'CG_TT
Cuts at: 5164 5164
Size: 5324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 2032 3149 3581 3934 4596 1451
Size: 581 1117 432 353 662 2179
Fragments arranged by size:
2179 1117 662 581 432 353
AflIII A'CryG_T
Cuts at: 2676 4476 4757 2676
Size: 1800 281 3243
Fragments arranged by size:
3243 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 138 2629 4067 138
Size: 2491 1438 1395
Fragments arranged by size:
2491 1438 1395
ApaLI G'TGCA_C
Cuts at: 577 4162 577
Size: 3585 1739
ApoI r'AATT_y
Cuts at: 490 533 600 758 802 955 1319 1639 2055
Size: 43 67 158 44 153 364 320 416
Cuts at: 2055 2179 2328 4677 4933 490
Size: 124 149 2349 256 881
Fragments arranged by size:
2349 881 416 364 320 256 158 153
149 124 67 44 43
AseI AT'TA_AT
Cuts at: 20 350 382 863 20
Size: 330 32 481 4481
Fragments arranged by size:
4481 481 330 32
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
AvaI C'yCGr_G
Cuts at: 201 2344 201
Size: 2143 3181
BamHI G'GATC_C
Cuts at: 1569 2349 1569
Size: 780 4544
BanI G'GyrC_C
Cuts at: 2340 2754 3002 3037 2340
Size: 414 248 35 4627
Fragments arranged by size:
4627 414 248 35
BanII G_rGCy'C
Cuts at: 2338 3368 2338
Size: 1030 4294
BbeI G_GCGC'C
Cuts at: 2758 3006 2758
Size: 248 5076
BbsI GAAGACnn'nnnn_
Cuts at: 2162 2162
Size: 5324
BbvCI CC'TCA_GC
Cuts at: 131 131
Size: 5324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 958 992 958
Size: 34 5290
BciVI GTATCCnnnnn_n'
Cuts at: 578 1408 3222 4278 5324 578
Size: 830 1814 1056 1046 578
Fragments arranged by size:
1814 1056 1046 830 578
BclI T'GATC_A
Cuts at: 411 678 2844 411
Size: 267 2166 2891
Fragments arranged by size:
2891 2166 267
BfrBI ATG'CAT
Cuts at: 2235 2235
Size: 5324
BglII A'GATC_T
Cuts at: 2839 4876 2839
Size: 2037 3287
BlpI GC'TnA_GC
Cuts at: 1157 2436 1157
Size: 1279 4045
Bme1580I G_kGCm'C
Cuts at: 581 2949 3042 4166 581
Size: 2368 93 1124 1739
Fragments arranged by size:
2368 1739 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2536 2617 2952 5096 2536
Size: 81 335 2144 2764
Fragments arranged by size:
2764 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 216 3691 216
Size: 3475 1849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1981 2244 646
Size: 1335 263 3726
Fragments arranged by size:
3726 1335 263
Bpu10I CC'TnA_GC
Cuts at: 131 131
Size: 5324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 3379 4093 4391 2500
Size: 879 714 298 3433
Fragments arranged by size:
3433 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5028 5028
Size: 5324
BsaAI yAC'GTr
Cuts at: 2677 2677
Size: 5324
BsaBI GATnn'nnATC
Cuts at: 6 677 989 2838 4875 6
Size: 671 312 1849 2037 455
Fragments arranged by size:
2037 1849 671 455 312
BsaHI Gr'CG_yC
Cuts at: 2755 3003 3705 5201 2755
Size: 248 702 1496 2878
Fragments arranged by size:
2878 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2513 3034 3712 4123 4270 4544 2513
Size: 521 678 411 147 274 3293
Fragments arranged by size:
3293 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 324 354 324
Size: 30 5294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1343 1343
Size: 5324
BseYI C'CCAG_C
Cuts at: 2749 3507 4172 4580 2749
Size: 758 665 408 3493
Fragments arranged by size:
3493 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 927
Size: 5324
BsiEI CG_ry'CG
Cuts at: 104 2866 2912 4142 104
Size: 2762 46 1230 1286
Fragments arranged by size:
2762 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 581 2338 3116 3306 4166 581
Size: 1757 778 190 860 1739
Fragments arranged by size:
1757 1739 860 778 190
BsmI GAATG_Cn'
Cuts at: 448 448
Size: 5324
Bsp1286I G_dGCh'C
Cuts at: 581 2338 2949 3042 3116 3306 3368 4166 581
Size: 1757 611 93 74 190 62 798 1739
Fragments arranged by size:
1757 1739 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1122 1890 2106 3756 1122
Size: 768 216 1650 2690
Fragments arranged by size:
2690 1650 768 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2374 2588 2890 3271 2374
Size: 214 302 381 4427
Fragments arranged by size:
4427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3616 3670 5154 5319 50
Size: 3566 54 1484 165 55
Fragments arranged by size:
3566 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1346 1994 2132 2294 3236 1346
Size: 648 138 162 942 3434
Fragments arranged by size:
3434 942 648 162 138
BsrFI r'CCGG_y
Cuts at: 3322 3503 3322
Size: 181 5143
BsrGI T'GTAC_A
Cuts at: 1619 1619
Size: 5324
BssSI C'ACGA_G
Cuts at: 3595 4303 3595
Size: 708 4616
BstAPI GCAn_nnn'nTGC
Cuts at: 2701 2701
Size: 5324
BstBI TT'CG_AA
Cuts at: 3685 3685
Size: 5324
BstXI CCAn_nnnn'nTGG
Cuts at: 1227 1227
Size: 5324
BstYI r'GATC_y
Cuts at: 1 1569 2313 2349 2816 2839 3174 3420 3726
Size: 1568 744 36 467 23 335 246 306
Cuts at: 3726 3738 3824 4876 1
Size: 12 86 1052 449
Fragments arranged by size:
1568 1052 744 467 449 335 306 246
86 36 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3274 3455 3618 3274
Size: 181 163 4980
Fragments arranged by size:
4980 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 2306 2567 1658
Size: 648 261 4415
Fragments arranged by size:
4415 648 261
ClaI AT'CG_AT
Cuts at: 267 267
Size: 5324
DraI TTT'AAA
Cuts at: 800 1316 2323 800
Size: 516 1007 3801
Fragments arranged by size:
3801 1007 516
DraIII CAC_nnn'GTG
Cuts at: 513 513
Size: 5324
DrdI GACnn_nn'nnGTC
Cuts at: 3030 4374 3030
Size: 1344 3980
EaeI y'GGCC_r
Cuts at: 541 2909 3083 3474 3501 4846 541
Size: 2368 174 391 27 1345 1019
Fragments arranged by size:
2368 1345 1019 391 174 27
EagI C'GGCC_G
Cuts at: 2909 2909
Size: 5324
EarI CTCTTCn'nnn_
Cuts at: 2099 3347 3557 2099
Size: 1248 210 3866
Fragments arranged by size:
3866 1248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3532 4274 4420 5134 3532
Size: 742 146 714 3722
Fragments arranged by size:
3722 742 714 146
EcoICRI GAG'CTC
Cuts at: 2336 2336
Size: 5324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1451 1981 2032 2244 3149 3581 3934 4596
Size: 805 530 51 212 905 432 353 662
Cuts at: 4596 646
Size: 1374
Fragments arranged by size:
1374 905 805 662 530 432 353 212
51
EcoO109I rG'GnC_Cy
Cuts at: 309 2463 309
Size: 2154 3170
EcoRI G'AATT_C
Cuts at: 600 955 2328 600
Size: 355 1373 3596
Fragments arranged by size:
3596 1373 355
EcoRV GAT'ATC
Cuts at: 1938 1938
Size: 5324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1105 3343 1105
Size: 2238 3086
FspI TGC'GCA
Cuts at: 3105 3105
Size: 5324
HaeII r_GCGC'y
Cuts at: 151 2758 3006 4236 5007 151
Size: 2607 248 1230 771 468
Fragments arranged by size:
2607 1230 771 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1335 1367 3166 3196 3198 3228 1335
Size: 32 1799 30 2 30 3431
Fragments arranged by size:
3431 1799 32 30 30 2
HincII GTy'rAC
Cuts at: 636 969 1064 2363 636
Size: 333 95 1299 3597
Fragments arranged by size:
3597 1299 333 95
KasI G'GCGC_C
Cuts at: 2754 3002 2754
Size: 248 5076
KpnI G_GTAC'C
Cuts at: 2344 2344
Size: 5324
MfeI C'AATT_G
Cuts at: 2193 2193
Size: 5324
MluI A'CGCG_T
Cuts at: 4757 4757
Size: 5324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4083 4267 4083
Size: 184 5140
MscI TGG'CCA
Cuts at: 543 3085 543
Size: 2542 2782
MslI CAynn'nnrTG
Cuts at: 1231 2108 2258 2299 3313 3440 1231
Size: 877 150 41 1014 127 3115
Fragments arranged by size:
3115 1014 877 150 127 41
MspA1I CmG'CkG
Cuts at: 135 2435 2749 3109 3893 4138 135
Size: 2300 314 360 784 245 1321
Fragments arranged by size:
2300 1321 784 360 314 245
NaeI GCC'GGC
Cuts at: 3505 3505
Size: 5324
NarI GG'CG_CC
Cuts at: 2755 3003 2755
Size: 248 5076
NcoI C'CATG_G
Cuts at: 107 107
Size: 5324
NdeI CA'TA_TG
Cuts at: 630 630
Size: 5324
NgoMIV G'CCGG_C
Cuts at: 3503 3503
Size: 5324
NsiI A_TGCA'T
Cuts at: 2237 2237
Size: 5324
NspI r_CATG'y
Cuts at: 2377 2877 4480 2377
Size: 500 1603 3221
Fragments arranged by size:
3221 1603 500
PacI TTA_AT'TAA
Cuts at: 1508 1508
Size: 5324
PciI A'CATG_T
Cuts at: 4476 4476
Size: 5324
PflMI CCAn_nnn'nTGG
Cuts at: 513 923 1391 513
Size: 410 468 4446
Fragments arranged by size:
4446 468 410
PfoI T'CCnGG_A
Cuts at: 662 1985 662
Size: 1323 4001
PmeI GTTT'AAAC
Cuts at: 2323 2323
Size: 5324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 5292
PpuMI rG'GwC_Cy
Cuts at: 309 309
Size: 5324
PsiI TTA'TAA
Cuts at: 1685 1685
Size: 5324
PspXI vC'TCGA_Gb
Cuts at: 201 201
Size: 5324
PstI C_TGCA'G
Cuts at: 981 2371 3056 981
Size: 1390 685 3249
Fragments arranged by size:
3249 1390 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5324
PvuII CAG'CTG
Cuts at: 135 2749 3109 135
Size: 2614 360 2350
Fragments arranged by size:
2614 2350 360
RsrII CG'GwC_CG
Cuts at: 3519 3519
Size: 5324
SacI G_AGCT'C
Cuts at: 2338 2338
Size: 5324
SalI G'TCGA_C
Cuts at: 967 2361 967
Size: 1394 3930
SapI GCTCTTCn'nnn_
Cuts at: 3347 3557 3347
Size: 210 5114
SbfI CC_TGCA'GG
Cuts at: 2371 2371
Size: 5324
ScaI AGT'ACT
Cuts at: 4841 4841
Size: 5324
SfcI C'TryA_G
Cuts at: 33 236 782 977 2367 3052 4020 4211 33
Size: 203 546 195 1390 685 968 191 1146
Fragments arranged by size:
1390 1146 968 685 546 203 195 191
SfoI GGC'GCC
Cuts at: 2756 3004 2756
Size: 248 5076
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
SmaI CCC'GGG
Cuts at: 2346 2346
Size: 5324
SmlI C'TyrA_G
Cuts at: 201 2479 3394 4108 4370 201
Size: 2278 915 714 262 1155
Fragments arranged by size:
2278 1155 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5324
SphI G_CATG'C
Cuts at: 2377 2877 2377
Size: 500 4824
SspI AAT'ATT
Cuts at: 289 289
Size: 5324
StyI C'CwwG_G
Cuts at: 107 312 1306 2458 2812 107
Size: 205 994 1152 354 2619
Fragments arranged by size:
2619 1152 994 354 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1597 2907 3040 3499 3708 169
Size: 1428 1310 133 459 209 1785
Fragments arranged by size:
1785 1428 1310 459 209 133
TatI w'GTAC_w
Cuts at: 1499 1619 4839 1499
Size: 120 3220 1984
Fragments arranged by size:
3220 1984 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 324 356 1243 1275 4841 4873 107
Size: 32 185 32 887 32 3566 32 558
Fragments arranged by size:
3566 887 558 185 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3121 3121
Size: 5324
XbaI T'CTAG_A
Cuts at: 2355 2355
Size: 5324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 772 1802 2219 2819 772
Size: 1030 417 600 3277
Fragments arranged by size:
3277 1030 600 417
XhoI C'TCGA_G
Cuts at: 201 201
Size: 5324
XmaI C'CCGG_G
Cuts at: 2344 2344
Size: 5324
XmnI GAAnn'nnTTC
Cuts at: 479 2159 2267 479
Size: 1680 108 3536
Fragments arranged by size:
3536 1680 108
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BglI BmgBI BmtI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I CspCI
EcoNI FseI FspAI HindIII HpaI NheI NotI NruI
PasI PmlI PshAI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI