Restriction map for
pF1KB5566
(Circular) (Six-base) MAPSORT of: pF1KB5566.seq Check: 5673 from: 1 to: 3905
pF1KB5566 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 853 943 853
Size: 90 3815
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1730 2162 2909 3177 1730
Size: 432 747 268 2458
Fragments arranged by size:
2458 747 432 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 797 2293 797
Size: 1496 2409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 781 781
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 687 1210 2777 687
Size: 523 1567 1815
Fragments arranged by size:
1815 1567 523
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 925 925
Size: 3905
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 369 402 369
Size: 33 3872
BamHI G'GATC_C
Cuts at: 204 325 930 204
Size: 121 605 3179
Fragments arranged by size:
3179 605 121
BanI G'GyrC_C
Cuts at: 397 921 1335 1583 1618 397
Size: 524 414 248 35 2684
Fragments arranged by size:
2684 524 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 884 1425 884
Size: 541 3364
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 680 721 1017 680
Size: 41 296 3568
Fragments arranged by size:
3568 296 41
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 876 1117 1198 1533 3677 876
Size: 241 81 335 2144 1104
Fragments arranged by size:
2144 1104 335 241 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1960 2452 2750 1081
Size: 879 492 298 2236
Fragments arranged by size:
2236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 853 3609 853
Size: 2756 1149
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 1419 3456 6
Size: 1413 2037 455
Fragments arranged by size:
2037 1413 455
BsaHI Gr'CG_yC
Cuts at: 211 1336 1584 2286 3782 211
Size: 1125 248 702 1496 334
Fragments arranged by size:
1496 1125 702 334 248
BsaWI w'CCGG_w
Cuts at: 133 797 1094 1615 2293 2567 2714 3125 133
Size: 664 297 521 678 274 147 411 913
Fragments arranged by size:
913 678 664 521 411 297 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 478
Size: 3905
BseYI C'CCAG_C
Cuts at: 321 393 1330 2088 2665 3161 321
Size: 72 937 758 577 496 1065
Fragments arranged by size:
1065 937 758 577 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 200
Size: 3905
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 919 1697 1887 2679 919
Size: 778 190 792 2145
Fragments arranged by size:
2145 792 778 190
BsmI GAATG_Cn'
Cuts at: 176 176
Size: 3905
Bsp1286I G_dGCh'C
Cuts at: 919 1530 1623 1697 1887 1949 2679 919
Size: 611 93 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 133 3125 133
Size: 2992 913
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1169 1471 1852 955
Size: 214 302 381 3008
Fragments arranged by size:
3008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 536 797 1903 2084 2293 536
Size: 261 1106 181 209 2148
Fragments arranged by size:
2148 1106 261 209 181
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 3905
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 717 1282 717
Size: 565 3340
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 3905
BstYI r'GATC_y
Cuts at: 1 204 325 930 1397 1420 1755 2001 3013
Size: 203 121 605 467 23 335 246 1012
Cuts at: 3013 3099 3111 3457 1
Size: 86 12 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 203
121 86 23 12
BtgI C'CryG_G
Cuts at: 107 254 569 107
Size: 147 315 3443
Fragments arranged by size:
3443 315 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 444 653 1490 1664 2055 2082 3427 444
Size: 209 837 174 391 27 1345 922
Fragments arranged by size:
1345 922 837 391 209 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 311 2113 2423 2569 3715 311
Size: 1802 310 146 1146 501
Fragments arranged by size:
1802 1146 501 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1730 2162 2909 3177 724
Size: 1006 432 747 268 1452
Fragments arranged by size:
1452 1006 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 266 1044 266
Size: 778 3127
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3905
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 543 1339 1587 2609 3588 543
Size: 796 248 1022 979 860
Fragments arranged by size:
1022 979 860 796 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 218 228 250 1747 1777 1779 1809 196
Size: 22 10 22 1497 30 2 30 2292
Fragments arranged by size:
2292 1497 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 378 944 378
Size: 566 3339
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 655 1666 655
Size: 1011 2894
MslI CAynn'nnrTG
Cuts at: 763 1894 2021 763
Size: 1131 127 2647
Fragments arranged by size:
2647 1131 127
MspA1I CmG'CkG
Cuts at: 1016 1330 1690 2703 2948 1016
Size: 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 360 314 245
NaeI GCC'GGC
Cuts at: 538 2086 538
Size: 1548 2357
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 107 254 569 107
Size: 147 315 3443
Fragments arranged by size:
3443 315 147
NgoMIV G'CCGG_C
Cuts at: 536 2084 536
Size: 1548 2357
NspI r_CATG'y
Cuts at: 321 624 762 958 1458 2365 321
Size: 303 138 196 500 907 1861
Fragments arranged by size:
1861 907 500 303 196 138
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PpuMI rG'GwC_Cy
Cuts at: 266 266
Size: 3905
PshAI GACnn'nnGTC
Cuts at: 271 849 271
Size: 578 3327
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 131 2100 131
Size: 1969 1936
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 489 854 948 1633 2626 2817 33
Size: 456 365 94 685 993 191 1121
Fragments arranged by size:
1121 993 685 456 365 191 94
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 1060 1975 2467 2729 1060
Size: 915 492 262 2236
Fragments arranged by size:
2236 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 762 958 1458 762
Size: 196 500 3209
Fragments arranged by size:
3209 500 196
StyI C'CwwG_G
Cuts at: 107 254 338 569 1039 1393 107
Size: 147 84 231 470 354 2619
Fragments arranged by size:
2619 470 354 231 147 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 745 1488 1621 2080 2289 385
Size: 360 743 133 459 209 2001
Fragments arranged by size:
2001 743 459 360 209 133
TatI w'GTAC_w
Cuts at: 530 770 3420 530
Size: 240 2650 1015
Fragments arranged by size:
2650 1015 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 886 1400 886
Size: 514 3391
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI