Restriction map for
pF1KB5569
(Circular) (Six-base) MAPSORT of: pF1KB5569.seq Check: 6628 from: 1 to: 4520
pF1KB5569 4520 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1558 1558
Size: 4520
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 379 816 2345 2777 3524 3792 259
Size: 120 437 1529 432 747 268 987
Fragments arranged by size:
1529 987 747 437 432 268 120
AflIII A'CryG_T
Cuts at: 1872 2976 3953 1872
Size: 1104 977 2439
Fragments arranged by size:
2439 1104 977
AgeI A'CCGG_T
Cuts at: 2908 2908
Size: 4520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1256 1256
Size: 4520
AlwNI CAG_nnn'CTG
Cuts at: 349 1825 3392 349
Size: 1476 1567 1477
Fragments arranged by size:
1567 1477 1476
ApaLI G'TGCA_C
Cuts at: 3290 3290
Size: 4520
ApoI r'AATT_y
Cuts at: 313 1105 1444 1466 1524 3873 4129 313
Size: 792 339 22 58 2349 256 704
Fragments arranged by size:
2349 792 704 339 256 58 22
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1540 1540
Size: 4520
BamHI G'GATC_C
Cuts at: 1329 1545 1329
Size: 216 4304
BanI G'GyrC_C
Cuts at: 1536 1950 2198 2233 1536
Size: 414 248 35 3823
Fragments arranged by size:
3823 414 248 35
BanII G_rGCy'C
Cuts at: 1049 1534 2564 1049
Size: 485 1030 3005
Fragments arranged by size:
3005 1030 485
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4486
BciVI GTATCCnnnnn_n'
Cuts at: 1177 2418 3179 4520 1177
Size: 1241 761 1341 1177
Fragments arranged by size:
1341 1241 1177 761
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 1086 1086
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 2145 2238 3294 2145
Size: 93 1056 3371
Fragments arranged by size:
3371 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 277 344 1161 1732 1813 2148 4292 277
Size: 67 817 571 81 335 2144 505
Fragments arranged by size:
2144 817 571 505 335 81 67
BmtI G_CTAG'C
Cuts at: 853 853
Size: 4520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 659 1320 1462 549
Size: 110 661 142 3607
Fragments arranged by size:
3607 661 142 110
Bpu10I CC'TnA_GC
Cuts at: 854 854
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1696 2575 3067 3365 1696
Size: 879 492 298 2851
Fragments arranged by size:
2851 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 567 4224 567
Size: 3657 863
BsaAI yAC'GTr
Cuts at: 1873 1873
Size: 4520
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 1951 2199 2901 4397 1951
Size: 248 702 1496 2074
Fragments arranged by size:
2074 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1127 1709 2230 2908 3182 3329 3740 1127
Size: 582 521 678 274 147 411 1907
Fragments arranged by size:
1907 678 582 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 261 291 261
Size: 30 4490
BseYI C'CCAG_C
Cuts at: 168 431 1945 2703 3280 3776 168
Size: 263 1514 758 577 496 912
Fragments arranged by size:
1514 912 758 577 496 263
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3316 104
Size: 1958 46 1208 1308
Fragments arranged by size:
1958 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1049 1534 2312 2502 3294 1049
Size: 485 778 190 792 2275
Fragments arranged by size:
2275 792 778 485 190
Bsp1286I G_dGCh'C
Cuts at: 1049 1534 2145 2238 2312 2502 2564 3294 1049
Size: 485 611 93 74 190 62 730 2275
Fragments arranged by size:
2275 730 611 485 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3740 3740
Size: 4520
BspHI T'CATG_A
Cuts at: 584 759 1397 3696 584
Size: 175 638 2299 1408
Fragments arranged by size:
2299 1408 638 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1570 1784 2086 2467 1570
Size: 214 302 381 3623
Fragments arranged by size:
3623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2432 2432
Size: 4520
BsrFI r'CCGG_y
Cuts at: 2518 2699 2908 2518
Size: 181 209 4130
Fragments arranged by size:
4130 209 181
BssSI C'ACGA_G
Cuts at: 2791 3149 2791
Size: 358 4162
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 1897
Size: 4520
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 778 778
Size: 4520
BstYI r'GATC_y
Cuts at: 1 1329 1545 2012 2035 2370 2616 3628 3714
Size: 1328 216 467 23 335 246 1012 86
Cuts at: 3714 3726 4072 1
Size: 12 346 449
Fragments arranged by size:
1328 1012 467 449 346 335 246 216
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 124 1763 124
Size: 1639 2881
ClaI AT'CG_AT
Cuts at: 1238 1238
Size: 4520
DraI TTT'AAA
Cuts at: 999 1519 999
Size: 520 4000
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3084 2226
Size: 858 3662
EaeI y'GGCC_r
Cuts at: 768 1069 1374 2105 2279 2670 2697 4042 768
Size: 301 305 731 174 391 27 1345 1246
Fragments arranged by size:
1345 1246 731 391 305 301 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 1456 2543 2753 1456
Size: 1087 210 3223
Fragments arranged by size:
3223 1087 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1047 1532 1047
Size: 485 4035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 379 549 659 816 1320 1462 2345 2777
Size: 120 170 110 157 504 142 883 432
Cuts at: 2777 3524 3792 259
Size: 747 268 987
Fragments arranged by size:
987 883 747 504 432 268 170 157
142 120 110
EcoNI CCTnn'n_nnAGG
Cuts at: 415 415
Size: 4520
EcoO109I rG'GnC_Cy
Cuts at: 1659 1659
Size: 4520
EcoRI G'AATT_C
Cuts at: 313 1524 313
Size: 1211 3309
EcoRV GAT'ATC
Cuts at: 590 801 590
Size: 211 4309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 972 2539 972
Size: 1567 2953
FspI TGC'GCA
Cuts at: 1018 2301 1018
Size: 1283 3237
FspAI rTGC'GCAy
Cuts at: 1018 1018
Size: 4520
HaeII r_GCGC'y
Cuts at: 1954 2202 3224 4203 1954
Size: 248 1022 979 2271
Fragments arranged by size:
2271 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1109 1141 2362 2392 2394 2424 1109
Size: 32 1221 30 2 30 3205
Fragments arranged by size:
3205 1221 32 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 546 3191 3375 546
Size: 2645 184 1691
Fragments arranged by size:
2645 1691 184
MscI TGG'CCA
Cuts at: 1071 1376 2281 1071
Size: 305 905 3310
Fragments arranged by size:
3310 905 305
MslI CAynn'nnrTG
Cuts at: 698 1238 2509 2636 698
Size: 540 1271 127 2582
Fragments arranged by size:
2582 1271 540 127
MspA1I CmG'CkG
Cuts at: 1631 1945 2305 3318 3563 1631
Size: 314 360 1013 245 2588
Fragments arranged by size:
2588 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NheI G'CTAG_C
Cuts at: 849 849
Size: 4520
NspI r_CATG'y
Cuts at: 1017 1573 2073 2980 1017
Size: 556 500 907 2557
Fragments arranged by size:
2557 907 556 500
PciI A'CATG_T
Cuts at: 2976 2976
Size: 4520
PflMI CCAn_nnn'nTGG
Cuts at: 679 679
Size: 4520
PfoI T'CCnGG_A
Cuts at: 1365 1365
Size: 4520
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4488
PstI C_TGCA'G
Cuts at: 1507 1567 2252 1507
Size: 60 685 3775
Fragments arranged by size:
3775 685 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 1945 2305 1945
Size: 360 4160
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 1049 1534 1049
Size: 485 4035
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 1150 4037 1150
Size: 2887 1633
SfcI C'TryA_G
Cuts at: 33 453 1200 1503 1563 2248 3241 3432 33
Size: 420 747 303 60 685 993 191 1121
Fragments arranged by size:
1121 993 747 685 420 303 191 60
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 1675 2590 3082 3344 1675
Size: 915 492 262 2851
Fragments arranged by size:
2851 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1573 2073 1573
Size: 500 4020
StyI C'CwwG_G
Cuts at: 557 1190 1654 2008 557
Size: 633 464 354 3069
Fragments arranged by size:
3069 633 464 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2103 2236 2695 2904 2103
Size: 133 459 209 3719
Fragments arranged by size:
3719 459 209 133
TatI w'GTAC_w
Cuts at: 734 1148 4035 734
Size: 414 2887 1219
Fragments arranged by size:
2887 1219 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 261 293 1157 1189 4037 4069 261
Size: 32 864 32 2848 32 712
Fragments arranged by size:
2848 864 712 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 205 2317 205
Size: 2112 2408
XbaI T'CTAG_A
Cuts at: 177 1551 177
Size: 1374 3146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 883 2015 883
Size: 1132 3388
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
XmnI GAAnn'nnTTC
Cuts at: 517 517
Size: 4520
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI CspCI DraIII FseI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI