Restriction map for
pF1KB5571
(Circular) (Six-base) MAPSORT of: pF1KB5571.seq Check: 1189 from: 1 to: 5867
pF1KB5571 5867 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 2452 2905 2452
Size: 453 5414
Acc65I G'GTAC_C
Cuts at: 1531 2883 1531
Size: 1352 4515
AclI AA'CG_TT
Cuts at: 5707 5707
Size: 5867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 2291 3692 4124 4871 5139 950
Size: 1341 1401 432 747 268 1678
Fragments arranged by size:
1678 1401 1341 747 432 268
AflIII A'CryG_T
Cuts at: 3219 4323 5300 3219
Size: 1104 977 3786
Fragments arranged by size:
3786 1104 977
AgeI A'CCGG_T
Cuts at: 4255 4255
Size: 5867
AleI CACnn'nnGTG
Cuts at: 2282 2282
Size: 5867
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1664 1696 1664
Size: 32 5835
AlwNI CAG_nnn'CTG
Cuts at: 2749 3172 4739 2749
Size: 423 1567 3877
Fragments arranged by size:
3877 1567 423
ApaLI G'TGCA_C
Cuts at: 4637 4637
Size: 5867
ApoI r'AATT_y
Cuts at: 498 1921 2871 5220 5476 498
Size: 1423 950 2349 256 889
Fragments arranged by size:
2349 1423 950 889 256
AseI AT'TA_AT
Cuts at: 20 2264 20
Size: 2244 3623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5867
AvaI C'yCGr_G
Cuts at: 2887 2887
Size: 5867
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1023 1056 1023
Size: 33 5834
BamHI G'GATC_C
Cuts at: 2892 2892
Size: 5867
BanI G'GyrC_C
Cuts at: 1531 2883 3297 3545 3580 1531
Size: 1352 414 248 35 3818
Fragments arranged by size:
3818 1352 414 248 35
BanII G_rGCy'C
Cuts at: 2881 3911 2881
Size: 1030 4837
BbeI G_GCGC'C
Cuts at: 3301 3549 3301
Size: 248 5619
BbsI GAAGACnn'nnnn_
Cuts at: 938 1168 2291 2427 938
Size: 230 1123 136 4378
Fragments arranged by size:
4378 1123 230 136
BciVI GTATCCnnnnn_n'
Cuts at: 1024 3765 4526 5867 1024
Size: 2741 761 1341 1024
Fragments arranged by size:
2741 1341 1024 761
BclI T'GATC_A
Cuts at: 797 3387 797
Size: 2590 3277
BfrBI ATG'CAT
Cuts at: 2306 2306
Size: 5867
BglII A'GATC_T
Cuts at: 2226 2490 3382 5419 2226
Size: 264 892 2037 2674
Fragments arranged by size:
2674 2037 892 264
BlpI GC'TnA_GC
Cuts at: 1986 2979 1986
Size: 993 4874
Bme1580I G_kGCm'C
Cuts at: 3492 3585 4641 3492
Size: 93 1056 4718
Fragments arranged by size:
4718 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2094 3079 3160 3495 5639 2094
Size: 985 81 335 2144 2322
Fragments arranged by size:
2322 2144 985 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4234 4234
Size: 5867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 467 1056 1065 1583 197
Size: 270 589 9 518 4481
Fragments arranged by size:
4481 589 518 270 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1115 1492 1934 2424 3043 3922 4414 4712 1115
Size: 377 442 490 619 879 492 298 2270
Fragments arranged by size:
2270 879 619 492 490 442 377 298
BsaI GGTCTCn'nnnn_
Cuts at: 2033 5571 2033
Size: 3538 2329
BsaAI yAC'GTr
Cuts at: 3220 3220
Size: 5867
BsaBI GATnn'nnATC
Cuts at: 6 1227 1292 3381 5418 6
Size: 1221 65 2089 2037 455
Fragments arranged by size:
2089 2037 1221 455 65
BsaHI Gr'CG_yC
Cuts at: 3298 3546 4248 5744 3298
Size: 248 702 1496 3421
Fragments arranged by size:
3421 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3056 3577 4255 4529 4676 5087 3056
Size: 521 678 274 147 411 3836
Fragments arranged by size:
3836 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1155 1185 1581 1611 1155
Size: 30 396 30 5411
Fragments arranged by size:
5411 396 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2108 2491 2108
Size: 383 5484
BseYI C'CCAG_C
Cuts at: 2456 3292 4050 4627 5123 2456
Size: 836 758 577 496 3200
Fragments arranged by size:
3200 836 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3409 3455 4663 104
Size: 3305 46 1208 1308
Fragments arranged by size:
3305 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2374 2881 3659 3849 4641 2374
Size: 507 778 190 792 3600
Fragments arranged by size:
3600 792 778 507 190
BsmI GAATG_Cn'
Cuts at: 717 1402 1990 717
Size: 685 588 4594
Fragments arranged by size:
4594 685 588
Bsp1286I G_dGCh'C
Cuts at: 2374 2881 3492 3585 3659 3849 3911 4641 2374
Size: 507 611 93 74 190 62 730 3600
Fragments arranged by size:
3600 730 611 507 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5087 5087
Size: 5867
BspHI T'CATG_A
Cuts at: 5043 5043
Size: 5867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2917 3131 3433 3814 2917
Size: 214 302 381 4970
Fragments arranged by size:
4970 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2481 4159 4213 5697 5862 50
Size: 2431 1678 54 1484 165 55
Fragments arranged by size:
2431 1678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1165 3779 1165
Size: 2614 3253
BsrFI r'CCGG_y
Cuts at: 3865 4046 4255 3865
Size: 181 209 5477
Fragments arranged by size:
5477 209 181
BsrGI T'GTAC_A
Cuts at: 219 1146 1868 219
Size: 927 722 4218
Fragments arranged by size:
4218 927 722
BssSI C'ACGA_G
Cuts at: 1153 4138 4496 1153
Size: 2985 358 2524
Fragments arranged by size:
2985 2524 358
BstAPI GCAn_nnn'nTGC
Cuts at: 712 2731 3244 712
Size: 2019 513 3335
Fragments arranged by size:
3335 2019 513
BstBI TT'CG_AA
Cuts at: 4228 4228
Size: 5867
BstEII G'GTnAC_C
Cuts at: 2105 2105
Size: 5867
BstXI CCAn_nnnn'nTGG
Cuts at: 1103 1103
Size: 5867
BstYI r'GATC_y
Cuts at: 1 183 1116 1392 2076 2226 2443 2490 2689
Size: 182 933 276 684 150 217 47 199
Cuts at: 2689 2892 3359 3382 3717 3963 4975 5061 5073
Size: 203 467 23 335 246 1012 86 12
Cuts at: 5073 5419 1
Size: 346 449
Fragments arranged by size:
1012 933 684 467 449 346 335 276
246 217 203 199 182 150 86 47
23 12
Bsu36I CC'TnA_GG
Cuts at: 332 2858 332
Size: 2526 3341
BtgI C'CryG_G
Cuts at: 2390 2390
Size: 5867
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1525 3817 3998 4161 1525
Size: 2292 181 163 3231
Fragments arranged by size:
3231 2292 181 163
BtsI GCAGTG_nn'
Cuts at: 239 2289 2343 3110 239
Size: 2050 54 767 2996
Fragments arranged by size:
2996 2050 767 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1138 1173 1138
Size: 35 5832
DraI TTT'AAA
Cuts at: 587 2866 587
Size: 2279 3588
DrdI GACnn_nn'nnGTC
Cuts at: 3573 4431 3573
Size: 858 5009
EaeI y'GGCC_r
Cuts at: 2482 3452 3626 4017 4044 5389 2482
Size: 970 174 391 27 1345 2960
Fragments arranged by size:
2960 1345 970 391 174 27
EagI C'GGCC_G
Cuts at: 3452 3452
Size: 5867
EarI CTCTTCn'nnn_
Cuts at: 2012 3890 4100 2012
Size: 1878 210 3779
Fragments arranged by size:
3779 1878 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 767 4075 4385 4531 5677 767
Size: 3308 310 146 1146 957
Fragments arranged by size:
3308 1146 957 310 146
EcoICRI GAG'CTC
Cuts at: 2879 2879
Size: 5867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 467 950 1056 1065 1583 2291 3692 4124
Size: 270 483 106 9 518 708 1401 432
Cuts at: 4124 4871 5139 197
Size: 747 268 925
Fragments arranged by size:
1401 925 747 708 518 483 432 270
268 106 9
EcoNI CCTnn'n_nnAGG
Cuts at: 721 1667 721
Size: 946 4921
EcoO109I rG'GnC_Cy
Cuts at: 1672 3006 1672
Size: 1334 4533
EcoRI G'AATT_C
Cuts at: 1921 2871 1921
Size: 950 4917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3886 3886
Size: 5867
FspI TGC'GCA
Cuts at: 3648 3648
Size: 5867
HaeII r_GCGC'y
Cuts at: 3301 3549 4571 5550 3301
Size: 248 1022 979 3618
Fragments arranged by size:
3618 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 799 831 925 957 1069 1101 1155 1187 1260
Size: 32 94 32 112 32 54 32 73
Cuts at: 1260 1292 2218 2250 2652 2684 3709 3739 3741
Size: 32 926 32 402 32 1025 30 2
Cuts at: 3741 3771 799
Size: 30 2895
Fragments arranged by size:
2895 1025 926 402 112 94 73 54
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 113 684 2906 113
Size: 571 2222 3074
Fragments arranged by size:
3074 2222 571
KasI G'GCGC_C
Cuts at: 3297 3545 3297
Size: 248 5619
KpnI G_GTAC'C
Cuts at: 1535 2887 1535
Size: 1352 4515
MluI A'CGCG_T
Cuts at: 5300 5300
Size: 5867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4538 4722 4538
Size: 184 5683
MscI TGG'CCA
Cuts at: 3628 3628
Size: 5867
MslI CAynn'nnrTG
Cuts at: 287 1972 2282 2513 3856 3983 287
Size: 1685 310 231 1343 127 2171
Fragments arranged by size:
2171 1685 1343 310 231 127
MspA1I CmG'CkG
Cuts at: 1342 2978 3292 3652 4665 4910 1342
Size: 1636 314 360 1013 245 2299
Fragments arranged by size:
2299 1636 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4048 4048
Size: 5867
NarI GG'CG_CC
Cuts at: 3298 3546 3298
Size: 248 5619
NgoMIV G'CCGG_C
Cuts at: 4046 4046
Size: 5867
NsiI A_TGCA'T
Cuts at: 2308 2308
Size: 5867
NspI r_CATG'y
Cuts at: 1422 2310 2811 2856 2920 3420 4327 1422
Size: 888 501 45 64 500 907 2962
Fragments arranged by size:
2962 907 888 501 500 64 45
PciI A'CATG_T
Cuts at: 4323 4323
Size: 5867
PmeI GTTT'AAAC
Cuts at: 2866 2866
Size: 5867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5006 5038 5006
Size: 32 5835
PpuMI rG'GwC_Cy
Cuts at: 1672 1672
Size: 5867
PsiI TTA'TAA
Cuts at: 865 865
Size: 5867
PstI C_TGCA'G
Cuts at: 246 2566 2727 2914 3599 246
Size: 2320 161 187 685 2514
Fragments arranged by size:
2514 2320 685 187 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5867
PvuII CAG'CTG
Cuts at: 1342 3292 3652 1342
Size: 1950 360 3557
Fragments arranged by size:
3557 1950 360
RsrII CG'GwC_CG
Cuts at: 4062 4062
Size: 5867
SacI G_AGCT'C
Cuts at: 2881 2881
Size: 5867
SalI G'TCGA_C
Cuts at: 2904 2904
Size: 5867
SapI GCTCTTCn'nnn_
Cuts at: 3890 4100 3890
Size: 210 5657
SbfI CC_TGCA'GG
Cuts at: 2914 2914
Size: 5867
ScaI AGT'ACT
Cuts at: 2548 5384 2548
Size: 2836 3031
SfcI C'TryA_G
Cuts at: 33 167 242 1137 2406 2562 2723 2910 3595
Size: 134 75 895 1269 156 161 187 685
Cuts at: 3595 4588 4779 33
Size: 993 191 1121
Fragments arranged by size:
1269 1121 993 895 685 191 187 161
156 134 75
SfoI GGC'GCC
Cuts at: 3299 3547 3299
Size: 248 5619
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5867
SmaI CCC'GGG
Cuts at: 2889 2889
Size: 5867
SmlI C'TyrA_G
Cuts at: 1130 1471 1949 2439 3022 3937 4429 4691 1130
Size: 341 478 490 583 915 492 262 2306
Fragments arranged by size:
2306 915 583 492 490 478 341 262
SpeI A'CTAG_T
Cuts at: 67 1855 67
Size: 1788 4079
SphI G_CATG'C
Cuts at: 2811 2920 3420 2811
Size: 109 500 5258
Fragments arranged by size:
5258 500 109
SspI AAT'ATT
Cuts at: 402 402
Size: 5867
StyI C'CwwG_G
Cuts at: 717 1444 2122 3001 3355 717
Size: 727 678 879 354 3229
Fragments arranged by size:
3229 879 727 678 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 3450 3583 4042 4251 1416
Size: 2034 133 459 209 3032
Fragments arranged by size:
3032 2034 459 209 133
TatI w'GTAC_w
Cuts at: 219 1146 1868 2546 5382 219
Size: 927 722 678 2836 704
Fragments arranged by size:
2836 927 722 704 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5384 5416 5384
Size: 32 5835
Tth111I GACn'n_nGTC
Cuts at: 3664 3664
Size: 5867
XbaI T'CTAG_A
Cuts at: 180 2898 180
Size: 2718 3149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3362 3362
Size: 5867
XmaI C'CCGG_G
Cuts at: 2887 2887
Size: 5867
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstZ17I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI