Restriction map for
pF1KB5572
(Circular) (Six-base) MAPSORT of: pF1KB5572.seq Check: 4822 from: 1 to: 4256
pF1KB5572 4256 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 190 190
Size: 4256
AccI GT'mk_AC
Cuts at: 1294 1294
Size: 4256
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 748 2081 2513 3260 3528 388
Size: 360 1333 432 747 268 1116
Fragments arranged by size:
1333 1116 747 432 360 268
AflIII A'CryG_T
Cuts at: 472 1608 2712 3689 472
Size: 1136 1104 977 1039
Fragments arranged by size:
1136 1104 1039 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 509
Size: 32 4224
AlwNI CAG_nnn'CTG
Cuts at: 404 1561 3128 404
Size: 1157 1567 1532
Fragments arranged by size:
1567 1532 1157
ApaLI G'TGCA_C
Cuts at: 468 3026 468
Size: 2558 1698
ApoI r'AATT_y
Cuts at: 1240 1260 3609 3865 1240
Size: 20 2349 256 1631
Fragments arranged by size:
2349 1631 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 213 349 484 1272 1686 1934 1969 213
Size: 136 135 788 414 248 35 2500
Fragments arranged by size:
2500 788 414 248 136 135 35
BanII G_rGCy'C
Cuts at: 115 387 1270 2300 115
Size: 272 883 1030 2071
Fragments arranged by size:
2071 1030 883 272
BbeI G_GCGC'C
Cuts at: 217 353 488 1690 1938 217
Size: 136 135 1202 248 2535
Fragments arranged by size:
2535 1202 248 136 135
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2915 4256 2154
Size: 761 1341 2154
Fragments arranged by size:
2154 1341 761
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglI GCCn_nnn'nGGC
Cuts at: 644 644
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 472 1881 1974 3030 472
Size: 1409 93 1056 1698
Fragments arranged by size:
1698 1409 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 813 1468 1549 1884 4028 813
Size: 655 81 335 2144 1041
Fragments arranged by size:
2144 1041 655 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 402 229
Size: 173 4083
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2311 2803 3101 1432
Size: 879 492 298 2587
Fragments arranged by size:
2587 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3960 3960
Size: 4256
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 214 350 485 1687 1935 2637 4133 214
Size: 136 135 1202 248 702 1496 337
Fragments arranged by size:
1496 1202 702 337 248 136 135
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 509
Size: 30 4226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 598
Size: 4256
BseYI C'CCAG_C
Cuts at: 654 1082 1681 2439 3016 3512 654
Size: 428 599 758 577 496 1398
Fragments arranged by size:
1398 758 599 577 496 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1059
Size: 4256
BsiEI CG_ry'CG
Cuts at: 104 1068 1798 1844 3052 104
Size: 964 730 46 1208 1308
Fragments arranged by size:
1308 1208 964 730 46
BsiHKAI G_wGCw'C
Cuts at: 115 387 472 826 1270 2048 2238 3030 115
Size: 272 85 354 444 778 190 792 1341
Fragments arranged by size:
1341 792 778 444 354 272 190 85
BsmI GAATG_Cn'
Cuts at: 343 497 343
Size: 154 4102
Bsp1286I G_dGCh'C
Cuts at: 115 387 472 826 1270 1881 1974 2048 2238
Size: 272 85 354 444 611 93 74 190
Cuts at: 2238 2300 3030 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 444 354 272 190 93
85 74 62
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 1148 3432 1148
Size: 2284 1972
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 1306 1520 1822 2203 190
Size: 1116 214 302 381 2243
Fragments arranged by size:
2243 1116 381 302 214
BsrBI CCG'CTC
Cuts at: 50 364 2548 2602 4086 4251 50
Size: 314 2184 54 1484 165 55
Fragments arranged by size:
2184 1484 314 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 1034 2254 2435 2644 1034
Size: 1220 181 209 2646
Fragments arranged by size:
2646 1220 209 181
BsrGI T'GTAC_A
Cuts at: 935 935
Size: 4256
BssHII G'CGCG_C
Cuts at: 224 224
Size: 4256
BssSI C'ACGA_G
Cuts at: 854 2527 2885 854
Size: 1673 358 2225
Fragments arranged by size:
2225 1673 358
BstAPI GCAn_nnn'nTGC
Cuts at: 962 1633 962
Size: 671 3585
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 333 333
Size: 4256
BstYI r'GATC_y
Cuts at: 1 374 1281 1748 1771 2106 2352 3364 3450
Size: 373 907 467 23 335 246 1012 86
Cuts at: 3450 3462 3808 1
Size: 12 346 449
Fragments arranged by size:
1012 907 467 449 373 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 488 620 488
Size: 132 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 105 2206 2387 2550 105
Size: 2101 181 163 1811
Fragments arranged by size:
2101 1811 181 163
BtsI GCAGTG_nn'
Cuts at: 198 580 1499 198
Size: 382 919 2955
Fragments arranged by size:
2955 919 382
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 1065 1841 2015 2406 2433 3778 1065
Size: 776 174 391 27 1345 1543
Fragments arranged by size:
1543 1345 776 391 174 27
EagI C'GGCC_G
Cuts at: 1065 1841 1065
Size: 776 3480
EarI CTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 385 1268 113
Size: 272 883 3101
Fragments arranged by size:
3101 883 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 388 402 748 2081 2513 3260 3528 229
Size: 159 14 346 1333 432 747 268 957
Fragments arranged by size:
1333 957 747 432 346 268 159 14
EcoNI CCTnn'n_nnAGG
Cuts at: 646 779 949 1215 646
Size: 133 170 266 3687
Fragments arranged by size:
3687 266 170 133
EcoO109I rG'GnC_Cy
Cuts at: 615 1395 615
Size: 780 3476
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 133 175 217 353 488 1690 1938 2960 3939
Size: 42 42 136 135 1202 248 1022 979
Cuts at: 3939 133
Size: 450
Fragments arranged by size:
1202 1022 979 450 248 136 135 42
42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2098 2128 2130 2160 2098
Size: 30 2 30 4194
Fragments arranged by size:
4194 30 30 2
HincII GTy'rAC
Cuts at: 683 1295 683
Size: 612 3644
KasI G'GCGC_C
Cuts at: 213 349 484 1686 1934 213
Size: 136 135 1202 248 2535
Fragments arranged by size:
2535 1202 248 136 135
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 690 2927 3111 690
Size: 2237 184 1835
Fragments arranged by size:
2237 1835 184
MscI TGG'CCA
Cuts at: 2017 2017
Size: 4256
MslI CAynn'nnrTG
Cuts at: 1193 2245 2372 1193
Size: 1052 127 3077
Fragments arranged by size:
3077 1052 127
MspA1I CmG'CkG
Cuts at: 190 1086 1115 1367 1681 2041 3054 3299 190
Size: 896 29 252 314 360 1013 245 1147
Fragments arranged by size:
1147 1013 896 360 314 252 245 29
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 214 350 485 1687 1935 214
Size: 136 135 1202 248 2535
Fragments arranged by size:
2535 1202 248 136 135
NcoI C'CATG_G
Cuts at: 620 620
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NspI r_CATG'y
Cuts at: 476 1192 1309 1809 2716 476
Size: 716 117 500 907 2016
Fragments arranged by size:
2016 907 716 500 117
PciI A'CATG_T
Cuts at: 472 2712 472
Size: 2240 2016
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 509 541 3395 3427 509
Size: 32 2854 32 1338
Fragments arranged by size:
2854 1338 32 32
PpuMI rG'GwC_Cy
Cuts at: 615 615
Size: 4256
PstI C_TGCA'G
Cuts at: 301 382 1303 1988 301
Size: 81 921 685 2569
Fragments arranged by size:
2569 921 685 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 1086 1681 2041 1086
Size: 595 360 3301
Fragments arranged by size:
3301 595 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 115 387 1270 115
Size: 272 883 3101
Fragments arranged by size:
3101 883 272
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 1045 3773 1045
Size: 2728 1528
SfcI C'TryA_G
Cuts at: 33 297 378 1215 1299 1984 2977 3168 33
Size: 264 81 837 84 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 264 191 84 81
SfoI GGC'GCC
Cuts at: 215 351 486 1688 1936 215
Size: 136 135 1202 248 2535
Fragments arranged by size:
2535 1202 248 136 135
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 1411 2326 2818 3080 1411
Size: 915 492 262 2587
Fragments arranged by size:
2587 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1192 1309 1809 1192
Size: 117 500 3639
Fragments arranged by size:
3639 500 117
StyI C'CwwG_G
Cuts at: 326 620 642 1390 1744 326
Size: 294 22 748 354 2838
Fragments arranged by size:
2838 748 354 294 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1839 1972 2431 2640 1839
Size: 133 459 209 3455
Fragments arranged by size:
3455 459 209 133
TatI w'GTAC_w
Cuts at: 935 1043 3771 935
Size: 108 2728 1420
Fragments arranged by size:
2728 1420 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 917 949 3773 3805 917
Size: 32 2824 32 1368
Fragments arranged by size:
2824 1368 32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 1751 552
Size: 1199 3057
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI