Restriction map for
pF1KB5573
(Circular) (Six-base) MAPSORT of: pF1KB5573.seq Check: 4042 from: 1 to: 6728
pF1KB5573 6728 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 330 1686 330
Size: 1356 5372
AccI GT'mk_AC
Cuts at: 276 3766 276
Size: 3490 3238
Acc65I G'GTAC_C
Cuts at: 3744 3744
Size: 6728
AclI AA'CG_TT
Cuts at: 6568 6568
Size: 6728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1765 2506 2531 3147 3289 3298 4553 4985
Size: 942 741 25 616 142 9 1255 432
Cuts at: 4985 5338 6000 823
Size: 353 662 1551
Fragments arranged by size:
1551 1255 942 741 662 616 432 353
142 25 9
AflIII A'CryG_T
Cuts at: 4080 5880 6161 4080
Size: 1800 281 4647
Fragments arranged by size:
4647 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2611 2611
Size: 6728
AleI CACnn'nnGTG
Cuts at: 2398 3007 2398
Size: 609 6119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1475 3688 1475
Size: 2213 4515
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 658 690 658
Size: 32 6696
AlwNI CAG_nnn'CTG
Cuts at: 3195 4033 5471 3195
Size: 838 1438 4452
Fragments arranged by size:
4452 1438 838
ApaLI G'TGCA_C
Cuts at: 5566 5566
Size: 6728
ApoI r'AATT_y
Cuts at: 2284 2926 3145 3511 3732 6081 6337 2284
Size: 642 219 366 221 2349 256 2675
Fragments arranged by size:
2675 2349 642 366 256 221 219
AseI AT'TA_AT
Cuts at: 20 239 20
Size: 219 6509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
AvaI C'yCGr_G
Cuts at: 1780 2216 3748 1780
Size: 436 1532 4760
Fragments arranged by size:
4760 1532 436
BamHI G'GATC_C
Cuts at: 3753 3753
Size: 6728
BanI G'GyrC_C
Cuts at: 1137 1882 2139 2813 2955 3295 3744 4158 4406
Size: 745 257 674 142 340 449 414 248
Cuts at: 4406 4441 1137
Size: 35 3424
Fragments arranged by size:
3424 745 674 449 414 340 257 248
142 35
BanII G_rGCy'C
Cuts at: 820 1994 2057 2789 3742 4772 820
Size: 1174 63 732 953 1030 2776
Fragments arranged by size:
2776 1174 1030 953 732 63
BbeI G_GCGC'C
Cuts at: 1886 2817 4162 4410 1886
Size: 931 1345 248 4204
Fragments arranged by size:
4204 1345 931 248
BbsI GAAGACnn'nnnn_
Cuts at: 1584 1584
Size: 6728
BbvCI CC'TCA_GC
Cuts at: 3178 3178
Size: 6728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1968 2002 1968
Size: 34 6694
BciVI GTATCCnnnnn_n'
Cuts at: 3415 4626 5682 6728 3415
Size: 1211 1056 1046 3415
Fragments arranged by size:
3415 1211 1056 1046
BclI T'GATC_A
Cuts at: 584 2747 4248 584
Size: 2163 1501 3064
Fragments arranged by size:
3064 2163 1501
BfrBI ATG'CAT
Cuts at: 2878 2878
Size: 6728
BglI GCCn_nnn'nGGC
Cuts at: 1377 2822 1377
Size: 1445 5283
BglII A'GATC_T
Cuts at: 321 1740 2342 4243 6280 321
Size: 1419 602 1901 2037 769
Fragments arranged by size:
2037 1901 1419 769 602
BlpI GC'TnA_GC
Cuts at: 3840 3840
Size: 6728
Bme1580I G_kGCm'C
Cuts at: 1781 3118 4353 4446 5570 1781
Size: 1337 1235 93 1124 2939
Fragments arranged by size:
2939 1337 1235 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3678 3940 4021 4356 6500 3678
Size: 262 81 335 2144 3906
Fragments arranged by size:
3906 2144 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5095 5095
Size: 6728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 454 1652 2708 346
Size: 108 1198 1056 4366
Fragments arranged by size:
4366 1198 1056 108
Bpu10I CC'TnA_GC
Cuts at: 2351 3178 2351
Size: 827 5901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4783 5497 5795 3904
Size: 879 714 298 4837
Fragments arranged by size:
4837 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1462 6432 1462
Size: 4970 1758
BsaAI yAC'GTr
Cuts at: 4081 4081
Size: 6728
BsaBI GATnn'nnATC
Cuts at: 6 3136 4242 6279 6
Size: 3130 1106 2037 455
Fragments arranged by size:
3130 2037 1106 455
BsaHI Gr'CG_yC
Cuts at: 1883 2814 4159 4407 5109 6605 1883
Size: 931 1345 248 702 1496 2006
Fragments arranged by size:
2006 1496 1345 931 702 248
BsaWI w'CCGG_w
Cuts at: 1925 2012 3917 4438 5116 5527 5674 5948 1925
Size: 87 1905 521 678 411 147 274 2705
Fragments arranged by size:
2705 1905 678 521 411 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 1274 1304 2611 2641 658
Size: 30 586 30 1307 30 4745
Fragments arranged by size:
4745 1307 586 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1798 3322 3538 3541 3577 1798
Size: 1524 216 3 36 4949
Fragments arranged by size:
4949 1524 216 36 3
BseYI C'CCAG_C
Cuts at: 2974 3286 4153 4911 5576 5984 2974
Size: 312 867 758 665 408 3718
Fragments arranged by size:
3718 867 758 665 408 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 2746 3694 162
Size: 2584 948 3196
Fragments arranged by size:
3196 2584 948
BsiEI CG_ry'CG
Cuts at: 104 4270 4316 5546 104
Size: 4166 46 1230 1286
Fragments arranged by size:
4166 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 820 1722 1837 3608 3742 4520 4710 5570 820
Size: 902 115 1771 134 778 190 860 1978
Fragments arranged by size:
1978 1771 902 860 778 190 134 115
BsmI GAATG_Cn'
Cuts at: 2240 2878 3458 2240
Size: 638 580 5510
Fragments arranged by size:
5510 638 580
Bsp1286I G_dGCh'C
Cuts at: 820 1722 1781 1837 1994 2057 2789 3118 3608
Size: 902 59 56 157 63 732 329 490
Cuts at: 3608 3742 4353 4446 4520 4710 4772 5570 820
Size: 134 611 93 74 190 62 798 1978
Fragments arranged by size:
1978 902 798 732 611 490 329 190
157 134 93 74 63 62 59 56
BspEI T'CCGG_A
Cuts at: 2012 2012
Size: 6728
BspHI T'CATG_A
Cuts at: 3497 5160 3497
Size: 1663 5065
BspMI ACCTGCnnnn'nnnn_
Cuts at: 330 1686 2430 3778 3992 4294 4675 330
Size: 1356 744 1348 214 302 381 2383
Fragments arranged by size:
2383 1356 1348 744 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5020 5074 6558 6723 50
Size: 4970 54 1484 165 55
Fragments arranged by size:
4970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 4640 2222
Size: 2418 4310
BsrFI r'CCGG_y
Cuts at: 1204 2291 2435 4726 4907 1204
Size: 1087 144 2291 181 3025
Fragments arranged by size:
3025 2291 1087 181 144
BsrGI T'GTAC_A
Cuts at: 258 1802 1871 3169 258
Size: 1544 69 1298 3817
Fragments arranged by size:
3817 1544 1298 69
BssSI C'ACGA_G
Cuts at: 4999 5707 4999
Size: 708 6020
BstAPI GCAn_nnn'nTGC
Cuts at: 4105 4105
Size: 6728
BstBI TT'CG_AA
Cuts at: 5089 5089
Size: 6728
BstXI CCAn_nnnn'nTGG
Cuts at: 147 1404 1608 147
Size: 1257 204 5267
Fragments arranged by size:
5267 1257 204
BstYI r'GATC_y
Cuts at: 1 321 1740 2078 2342 3047 3377 3753 4220
Size: 320 1419 338 264 705 330 376 467
Cuts at: 4220 4243 4578 4824 5130 5142 5228 6280 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1419 1052 705 467 449 376 338 335
330 320 306 264 246 86 23 12
BtgI C'CryG_G
Cuts at: 1285 1472 1994 2510 3051 1285
Size: 187 522 516 541 4962
Fragments arranged by size:
4962 541 522 516 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2502 4678 4859 5022 2502
Size: 2176 181 163 4208
Fragments arranged by size:
4208 2176 181 163
BtsI GCAGTG_nn'
Cuts at: 655 1946 2600 3971 655
Size: 1291 654 1371 3412
Fragments arranged by size:
3412 1371 1291 654
DraI TTT'AAA
Cuts at: 3727 3727
Size: 6728
DraIII CAC_nnn'GTG
Cuts at: 357 357
Size: 6728
DrdI GACnn_nn'nnGTC
Cuts at: 1618 4434 5778 1618
Size: 2816 1344 2568
Fragments arranged by size:
2816 2568 1344
EaeI y'GGCC_r
Cuts at: 149 906 2666 2777 2823 4313 4487 4878 4905
Size: 757 1760 111 46 1490 174 391 27
Cuts at: 4905 6250 149
Size: 1345 627
Fragments arranged by size:
1760 1490 1345 757 627 391 174 111
46 27
EagI C'GGCC_G
Cuts at: 4313 4313
Size: 6728
EarI CTCTTCn'nnn_
Cuts at: 413 3028 3211 3554 4751 4961 413
Size: 2615 183 343 1197 210 2180
Fragments arranged by size:
2615 2180 1197 343 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4936 5678 5824 6538 4936
Size: 742 146 714 5126
Fragments arranged by size:
5126 742 714 146
EcoICRI GAG'CTC
Cuts at: 818 3740 818
Size: 2922 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 454 823 1652 1765 2506 2531 2708 3147
Size: 108 369 829 113 741 25 177 439
Cuts at: 3147 3289 3298 4553 4985 5338 6000 346
Size: 142 9 1255 432 353 662 1074
Fragments arranged by size:
1255 1074 829 741 662 439 432 369
353 177 142 113 108 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 296 3280 3553 296
Size: 2984 273 3471
Fragments arranged by size:
3471 2984 273
EcoO109I rG'GnC_Cy
Cuts at: 884 2121 2361 3393 3867 884
Size: 1237 240 1032 474 3745
Fragments arranged by size:
3745 1237 1032 474 240
EcoRI G'AATT_C
Cuts at: 2284 3511 3732 2284
Size: 1227 221 5280
Fragments arranged by size:
5280 1227 221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 2883 3084 4747 402
Size: 2481 201 1663 2383
Fragments arranged by size:
2481 2383 1663 201
FspI TGC'GCA
Cuts at: 1822 1903 4509 1822
Size: 81 2606 4041
Fragments arranged by size:
4041 2606 81
HaeII r_GCGC'y
Cuts at: 1886 2817 4162 4410 5640 6411 1886
Size: 931 1345 248 1230 771 2203
Fragments arranged by size:
2203 1345 1230 931 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1274 1306 1650 1682 2597 2611 2629
Size: 32 440 32 344 32 915 14 18
Cuts at: 2629 2643 2681 2713 3362 3394 4570 4600 4602
Size: 14 38 32 649 32 1176 30 2
Cuts at: 4602 4632 802
Size: 30 2898
Fragments arranged by size:
2898 1176 915 649 440 344 38 32
32 32 32 32 30 30 18 14
14 2
HincII GTy'rAC
Cuts at: 697 936 1517 3767 697
Size: 239 581 2250 3658
Fragments arranged by size:
3658 2250 581 239
HindIII A'AGCT_T
Cuts at: 1544 2353 1544
Size: 809 5919
KasI G'GCGC_C
Cuts at: 1882 2813 4158 4406 1882
Size: 931 1345 248 4204
Fragments arranged by size:
4204 1345 931 248
KpnI G_GTAC'C
Cuts at: 3748 3748
Size: 6728
MluI A'CGCG_T
Cuts at: 6161 6161
Size: 6728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3593 3668 5487 5671 3593
Size: 75 1819 184 4650
Fragments arranged by size:
4650 1819 184 75
MscI TGG'CCA
Cuts at: 2779 4489 2779
Size: 1710 5018
MslI CAynn'nnrTG
Cuts at: 184 592 1457 1534 2398 2854 3007 4717 4844
Size: 408 865 77 864 456 153 1710 127
Cuts at: 4844 184
Size: 2068
Fragments arranged by size:
2068 1710 865 864 456 408 153 127
77
MspA1I CmG'CkG
Cuts at: 925 3839 4153 4513 5297 5542 925
Size: 2914 314 360 784 245 2111
Fragments arranged by size:
2914 2111 784 360 314 245
NaeI GCC'GGC
Cuts at: 4909 4909
Size: 6728
NarI GG'CG_CC
Cuts at: 1883 2814 4159 4407 1883
Size: 931 1345 248 4204
Fragments arranged by size:
4204 1345 931 248
NcoI C'CATG_G
Cuts at: 1285 1472 1994 2510 3051 1285
Size: 187 522 516 541 4962
Fragments arranged by size:
4962 541 522 516 187
NdeI CA'TA_TG
Cuts at: 3633 3633
Size: 6728
NgoMIV G'CCGG_C
Cuts at: 4907 4907
Size: 6728
NsiI A_TGCA'T
Cuts at: 2880 2880
Size: 6728
NspI r_CATG'y
Cuts at: 3781 4281 5884 3781
Size: 500 1603 4625
Fragments arranged by size:
4625 1603 500
PciI A'CATG_T
Cuts at: 5880 5880
Size: 6728
PflMI CCAn_nnn'nTGG
Cuts at: 894 894
Size: 6728
PfoI T'CCnGG_A
Cuts at: 431 1607 1919 3548 431
Size: 1176 312 1629 3611
Fragments arranged by size:
3611 1629 1176 312
PmeI GTTT'AAAC
Cuts at: 3727 3727
Size: 6728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 658 690 5174 5206 658
Size: 32 4484 32 2180
Fragments arranged by size:
4484 2180 32 32
PpuMI rG'GwC_Cy
Cuts at: 884 2121 2361 884
Size: 1237 240 5251
Fragments arranged by size:
5251 1237 240
PshAI GACnn'nnGTC
Cuts at: 889 889
Size: 6728
PstI C_TGCA'G
Cuts at: 274 321 650 774 1166 2642 3775 4460 274
Size: 47 329 124 392 1476 1133 685 2542
Fragments arranged by size:
2542 1476 1133 685 392 329 124 47
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6728
PvuII CAG'CTG
Cuts at: 925 4153 4513 925
Size: 3228 360 3140
Fragments arranged by size:
3228 3140 360
RsrII CG'GwC_CG
Cuts at: 4923 4923
Size: 6728
SacI G_AGCT'C
Cuts at: 820 3742 820
Size: 2922 3806
SalI G'TCGA_C
Cuts at: 3765 3765
Size: 6728
SanDI GG'GwC_CC
Cuts at: 884 884
Size: 6728
SapI GCTCTTCn'nnn_
Cuts at: 413 3028 4751 4961 413
Size: 2615 1723 210 2180
Fragments arranged by size:
2615 2180 1723 210
SbfI CC_TGCA'GG
Cuts at: 3775 3775
Size: 6728
ScaI AGT'ACT
Cuts at: 1321 6245 1321
Size: 4924 1804
SfcI C'TryA_G
Cuts at: 33 270 317 646 770 1162 2638 3104 3700
Size: 237 47 329 124 392 1476 466 596
Cuts at: 3700 3771 4456 5424 5615 33
Size: 71 685 968 191 1146
Fragments arranged by size:
1476 1146 968 685 596 466 392 329
237 191 124 71 47
SfoI GGC'GCC
Cuts at: 1884 2815 4160 4408 1884
Size: 931 1345 248 4204
Fragments arranged by size:
4204 1345 931 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6728
SmaI CCC'GGG
Cuts at: 2218 3750 2218
Size: 1532 5196
SmlI C'TyrA_G
Cuts at: 3883 4798 5512 5774 3883
Size: 915 714 262 4837
Fragments arranged by size:
4837 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6728
SphI G_CATG'C
Cuts at: 3781 4281 3781
Size: 500 6228
SrfI GCCC'GGGC
Cuts at: 2218 2218
Size: 6728
StuI AGG'CCT
Cuts at: 1685 1685
Size: 6728
StyI C'CwwG_G
Cuts at: 789 1285 1472 1994 2510 3051 3317 3862 4216
Size: 496 187 522 516 541 266 545 354
Cuts at: 4216 789
Size: 3301
Fragments arranged by size:
3301 545 541 522 516 496 354 266
187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1726 2988 4311 4444 4903 5112 1726
Size: 1262 1323 133 459 209 3342
Fragments arranged by size:
3342 1323 1262 459 209 133
TatI w'GTAC_w
Cuts at: 258 1300 1319 1353 1802 1871 3169 6243 258
Size: 1042 19 34 449 69 1298 3074 743
Fragments arranged by size:
3074 1298 1042 743 449 69 34 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 373 6245 6277 341
Size: 32 5872 32 792
Fragments arranged by size:
5872 792 32 32
Tth111I GACn'n_nGTC
Cuts at: 127 841 4525 127
Size: 714 3684 2330
Fragments arranged by size:
3684 2330 714
XbaI T'CTAG_A
Cuts at: 3759 3759
Size: 6728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 4223 453
Size: 3770 2958
XmaI C'CCGG_G
Cuts at: 2216 3748 2216
Size: 1532 5196
XmnI GAAnn'nnTTC
Cuts at: 407 634 3458 407
Size: 227 2824 3677
Fragments arranged by size:
3677 2824 227
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI ZraI