Restriction map for
pF1KB5574
(Circular) (Six-base) MAPSORT of: pF1KB5574.seq Check: 3777 from: 1 to: 3749
pF1KB5574 3749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 359 787 359
Size: 428 3321
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1574 2006 2753 3021 730
Size: 844 432 747 268 1458
Fragments arranged by size:
1458 844 747 432 268
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 655 1054 2621 655
Size: 399 1567 1783
Fragments arranged by size:
1783 1567 399
ApaLI G'TGCA_C
Cuts at: 223 2519 223
Size: 2296 1453
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 265 285 769 265
Size: 20 484 3245
Fragments arranged by size:
3245 484 20
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 763 1793 763
Size: 1030 2719
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BbsI GAAGACnn'nnnn_
Cuts at: 126 126
Size: 3749
BbvCI CC'TCA_GC
Cuts at: 299 299
Size: 3749
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 227 1374 1467 2523 227
Size: 1147 93 1056 1453
Fragments arranged by size:
1453 1147 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 358 276
Size: 82 3667
Bpu10I CC'TnA_GC
Cuts at: 299 299
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 925 1804 2296 2594 616
Size: 309 879 492 298 1771
Fragments arranged by size:
1771 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 222 464 1102 222
Size: 242 638 2869
Fragments arranged by size:
2869 638 242
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 155 291 1180 1428 2130 3626 155
Size: 136 889 248 702 1496 278
Fragments arranged by size:
1496 889 702 278 248 136
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 117 117
Size: 3749
BseYI C'CCAG_C
Cuts at: 207 352 530 1174 1932 2509 3005 207
Size: 145 178 644 758 577 496 951
Fragments arranged by size:
951 758 644 577 496 178 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 668 367
Size: 301 3448
BsiEI CG_ry'CG
Cuts at: 104 702 1291 1337 2545 104
Size: 598 589 46 1208 1308
Fragments arranged by size:
1308 1208 598 589 46
BsiHKAI G_wGCw'C
Cuts at: 227 763 1541 1731 2523 227
Size: 536 778 190 792 1453
Fragments arranged by size:
1453 792 778 536 190
BsmBI CGTCTCn'nnnn_
Cuts at: 600 600
Size: 3749
Bsp1286I G_dGCh'C
Cuts at: 227 763 1374 1467 1541 1731 1793 2523 227
Size: 536 611 93 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 536 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 167 665 2041 2095 3579 3744 50
Size: 117 498 1376 54 1484 165 55
Fragments arranged by size:
1484 1376 498 165 117 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 244 279 1747 1928 2137 244
Size: 35 1468 181 209 1856
Fragments arranged by size:
1856 1468 209 181 35
BssHII G'CGCG_C
Cuts at: 240 260 240
Size: 20 3729
BssSI C'ACGA_G
Cuts at: 553 2020 2378 553
Size: 1467 358 1924
Fragments arranged by size:
1924 1467 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstEII G'GTnAC_C
Cuts at: 411 411
Size: 3749
BstXI CCAn_nnnn'nTGG
Cuts at: 207 207
Size: 3749
BstYI r'GATC_y
Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955
Size: 773 467 23 335 246 1012 86 12
Cuts at: 2955 3301 1
Size: 346 449
Fragments arranged by size:
1012 773 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 431 497 509 539 107
Size: 324 66 12 30 3317
Fragments arranged by size:
3317 324 66 30 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 344 1699 1880 2043 344
Size: 1355 181 163 2050
Fragments arranged by size:
2050 1355 181 163
BtsI GCAGTG_nn'
Cuts at: 992 992
Size: 3749
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 428 438 494 506 699 1334 1508 1899 1926
Size: 10 56 12 193 635 174 391 27
Cuts at: 1926 3271 428
Size: 1345 906
Fragments arranged by size:
1345 906 635 391 193 174 56 27
12 10
EagI C'GGCC_G
Cuts at: 699 1334 699
Size: 635 3114
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 629 1957 2267 2413 3559 629
Size: 1328 310 146 1146 819
Fragments arranged by size:
1328 1146 819 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 358 730 1574 2006 2753 3021 276
Size: 82 372 844 432 747 268 1004
Fragments arranged by size:
1004 844 747 432 372 268 82
EcoNI CCTnn'n_nnAGG
Cuts at: 298 298
Size: 3749
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 735 1768 735
Size: 1033 2716
FspI TGC'GCA
Cuts at: 580 1530 580
Size: 950 2799
HaeII r_GCGC'y
Cuts at: 126 208 1183 1431 2453 3432 126
Size: 82 975 248 1022 979 443
Fragments arranged by size:
1022 979 975 443 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1621 1623 1653 1591
Size: 30 2 30 3687
Fragments arranged by size:
3687 30 30 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 440 1510 440
Size: 1070 2679
MslI CAynn'nnrTG
Cuts at: 112 1738 1865 112
Size: 1626 127 1996
Fragments arranged by size:
1996 1626 127
MspA1I CmG'CkG
Cuts at: 381 499 860 1174 1534 2547 2792 381
Size: 118 361 314 360 1013 245 1338
Fragments arranged by size:
1338 1013 361 360 314 245 118
NaeI GCC'GGC
Cuts at: 246 1930 246
Size: 1684 2065
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NcoI C'CATG_G
Cuts at: 107 539 107
Size: 432 3317
NgoMIV G'CCGG_C
Cuts at: 244 1928 244
Size: 1684 2065
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PflMI CCAn_nnn'nTGG
Cuts at: 675 675
Size: 3749
PfoI T'CCnGG_A
Cuts at: 253 253
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PmlI CAC'GTG
Cuts at: 464 464
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PshAI GACnn'nnGTC
Cuts at: 625 625
Size: 3749
PstI C_TGCA'G
Cuts at: 398 456 694 796 1481 398
Size: 58 238 102 685 2666
Fragments arranged by size:
2666 685 238 102 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 381 1174 1534 381
Size: 793 360 2596
Fragments arranged by size:
2596 793 360
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SacII CC_GC'GG
Cuts at: 500 500
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 394 452 690 792 1477 2470 2661 33
Size: 361 58 238 102 685 993 191 1121
Fragments arranged by size:
1121 993 685 361 238 191 102 58
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 287 771 287
Size: 484 3265
SmlI C'TyrA_G
Cuts at: 631 904 1819 2311 2573 631
Size: 273 915 492 262 1807
Fragments arranged by size:
1807 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 107 200 539 883 1237 107
Size: 93 339 344 354 2619
Fragments arranged by size:
2619 354 344 339 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 120 430 1332 1465 1924 2133 120
Size: 310 902 133 459 209 1736
Fragments arranged by size:
1736 902 459 310 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XmaI C'CCGG_G
Cuts at: 285 769 285
Size: 484 3265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PpuMI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI