Restriction map for
pF1KB5582
(Circular) (Six-base) MAPSORT of: pF1KB5582.seq Check: 646 from: 1 to: 4235
pF1KB5582 4235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 484 1251 484
Size: 767 3468
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 980 2060 2492 2845 3507 376
Size: 604 1080 432 353 662 1104
Fragments arranged by size:
1104 1080 662 604 432 353
AflIII A'CryG_T
Cuts at: 1587 3387 3668 1587
Size: 1800 281 2154
Fragments arranged by size:
2154 1800 281
AgeI A'CCGG_T
Cuts at: 640 640
Size: 4235
AleI CACnn'nnGTG
Cuts at: 559 559
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 1540 2978 1540
Size: 1438 2797
ApaLI G'TGCA_C
Cuts at: 3073 3073
Size: 4235
ApoI r'AATT_y
Cuts at: 409 790 1138 1239 3588 3844 409
Size: 381 348 101 2349 256 800
Fragments arranged by size:
2349 800 381 348 256 101
AseI AT'TA_AT
Cuts at: 20 472 20
Size: 452 3783
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 1255 1255
Size: 4235
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 189 484 1251 1665 1913 1948 189
Size: 295 767 414 248 35 2476
Fragments arranged by size:
2476 767 414 295 248 35
BanII G_rGCy'C
Cuts at: 1249 2279 1249
Size: 1030 3205
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3189 4235 2133
Size: 1056 1046 2133
Fragments arranged by size:
2133 1056 1046
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BfrBI ATG'CAT
Cuts at: 1194 1194
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 1860 1953 3077 1860
Size: 93 1124 3018
Fragments arranged by size:
3018 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1447 1528 1863 4007 1447
Size: 81 335 2144 1675
Fragments arranged by size:
2144 1675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 699 2602 699
Size: 1903 2332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 964 1411 2290 3004 3302 443
Size: 521 447 879 714 298 1376
Fragments arranged by size:
1376 879 714 521 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 968 1666 1914 2616 4112 968
Size: 698 248 702 1496 1091
Fragments arranged by size:
1496 1091 702 698 248
BsaWI w'CCGG_w
Cuts at: 640 1424 1945 2623 3034 3181 3455 640
Size: 784 521 678 411 147 274 1420
Fragments arranged by size:
1420 784 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 725 902 190
Size: 535 177 3523
Fragments arranged by size:
3523 535 177
BseYI C'CCAG_C
Cuts at: 962 1660 2418 3083 3491 962
Size: 698 758 665 408 1706
Fragments arranged by size:
1706 758 698 665 408
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3053 104
Size: 1673 46 1230 1286
Fragments arranged by size:
1673 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 869 1249 2027 2217 3077 869
Size: 380 778 190 860 2027
Fragments arranged by size:
2027 860 778 380 190
BsmI GAATG_Cn'
Cuts at: 409 1194 1208 409
Size: 785 14 3436
Fragments arranged by size:
3436 785 14
Bsp1286I G_dGCh'C
Cuts at: 869 1249 1860 1953 2027 2217 2279 3077 869
Size: 380 611 93 74 190 62 798 2027
Fragments arranged by size:
2027 798 611 380 190 93 74 62
BspHI T'CATG_A
Cuts at: 204 929 2667 204
Size: 725 1738 1772
Fragments arranged by size:
1772 1738 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1285 1499 1801 2182 1285
Size: 214 302 381 3338
Fragments arranged by size:
3338 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2527 2581 4065 4230 50
Size: 2477 54 1484 165 55
Fragments arranged by size:
2477 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 2147 224
Size: 1923 2312
BsrFI r'CCGG_y
Cuts at: 640 2233 2414 640
Size: 1593 181 2461
Fragments arranged by size:
2461 1593 181
BssSI C'ACGA_G
Cuts at: 2506 3214 2506
Size: 708 3527
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 213 1212 2596 213
Size: 999 1384 1852
Fragments arranged by size:
1852 1384 999
BstYI r'GATC_y
Cuts at: 1 588 1260 1727 1750 2085 2331 2637 2649
Size: 587 672 467 23 335 246 306 12
Cuts at: 2649 2735 3787 1
Size: 86 1052 449
Fragments arranged by size:
1052 672 587 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 278 836 278
Size: 558 3677
BtgI C'CryG_G
Cuts at: 107 107
Size: 4235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 230 662 1055 1478 230
Size: 432 393 423 2987
Fragments arranged by size:
2987 432 423 393
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DraIII CAC_nnn'GTG
Cuts at: 397 397
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 3285 1941
Size: 1344 2891
EaeI y'GGCC_r
Cuts at: 1820 1994 2385 2412 3757 1820
Size: 174 391 27 1345 2298
Fragments arranged by size:
2298 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 1069 2258 2468 1069
Size: 1189 210 2836
Fragments arranged by size:
2836 1189 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 3185 3331 4045 2443
Size: 742 146 714 2633
Fragments arranged by size:
2633 742 714 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 980 2060 2492 2845 3507 376
Size: 604 1080 432 353 662 1104
Fragments arranged by size:
1104 1080 662 604 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1374 1374
Size: 4235
EcoRI G'AATT_C
Cuts at: 1239 1239
Size: 4235
EcoRV GAT'ATC
Cuts at: 570 570
Size: 4235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 634 2254 634
Size: 1620 2615
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 1669 1917 3147 3918 1669
Size: 248 1230 771 1986
Fragments arranged by size:
1986 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1137 1169 2077 2107 2109 2139 1137
Size: 32 908 30 2 30 3233
Fragments arranged by size:
3233 908 32 30 30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 488 1255 488
Size: 767 3468
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 696 963 2994 3178 653
Size: 43 267 2031 184 1710
Fragments arranged by size:
2031 1710 267 184 43
MscI TGG'CCA
Cuts at: 1996 1996
Size: 4235
MslI CAynn'nnrTG
Cuts at: 248 311 559 769 874 2224 2351 248
Size: 63 248 210 105 1350 127 2132
Fragments arranged by size:
2132 1350 248 210 127 105 63
MspA1I CmG'CkG
Cuts at: 1029 1346 1660 2020 2804 3049 1029
Size: 317 314 360 784 245 2215
Fragments arranged by size:
2215 784 360 317 314 245
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 4235
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NcoI C'CATG_G
Cuts at: 107 107
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 4235
NsiI A_TGCA'T
Cuts at: 1196 1196
Size: 4235
NspI r_CATG'y
Cuts at: 953 1288 1788 3391 953
Size: 335 500 1603 1797
Fragments arranged by size:
1797 1603 500 335
PciI A'CATG_T
Cuts at: 3387 3387
Size: 4235
PflMI CCAn_nnn'nTGG
Cuts at: 397 397
Size: 4235
PfoI T'CCnGG_A
Cuts at: 757 857 757
Size: 100 4135
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 2681
Size: 32 4203
PstI C_TGCA'G
Cuts at: 141 1282 1967 141
Size: 1141 685 2409
Fragments arranged by size:
2409 1141 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1029 1660 2020 1029
Size: 631 360 3244
Fragments arranged by size:
3244 631 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SfcI C'TryA_G
Cuts at: 33 137 1278 1963 2931 3122 33
Size: 104 1141 685 968 191 1146
Fragments arranged by size:
1146 1141 968 685 191 104
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 458 943 1390 2305 3019 3281 458
Size: 485 447 915 714 262 1412
Fragments arranged by size:
1412 915 714 485 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
StyI C'CwwG_G
Cuts at: 107 1369 1723 107
Size: 1262 354 2619
Fragments arranged by size:
2619 1262 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 895 1818 1951 2410 2619 895
Size: 923 133 459 209 2511
Fragments arranged by size:
2511 923 459 209 133
TatI w'GTAC_w
Cuts at: 3750 3750
Size: 4235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 371 403 753 785 3752 3784 371
Size: 32 350 32 2967 32 822
Fragments arranged by size:
2967 822 350 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 370 2032 370
Size: 1662 2573
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1730 1730
Size: 4235
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI