Restriction map for
pF1KB5584
(Circular) (Six-base) MAPSORT of: pF1KB5584.seq Check: 5448 from: 1 to: 5036
pF1KB5584 5036 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2074 2074
Size: 5036
Acc65I G'GTAC_C
Cuts at: 1724 2052 1724
Size: 328 4708
AclI AA'CG_TT
Cuts at: 4876 4876
Size: 5036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2861 3293 3646 4308 1189
Size: 1672 432 353 662 1917
Fragments arranged by size:
1917 1672 662 432 353
AflIII A'CryG_T
Cuts at: 336 2388 4188 4469 336
Size: 2052 1800 281 903
Fragments arranged by size:
2052 1800 903 281
AlwNI CAG_nnn'CTG
Cuts at: 2341 3779 2341
Size: 1438 3598
ApaLI G'TGCA_C
Cuts at: 3874 3874
Size: 5036
ApoI r'AATT_y
Cuts at: 437 503 515 832 882 1047 1111 2040 4389
Size: 66 12 317 50 165 64 929 2349
Cuts at: 4389 4645 437
Size: 256 828
Fragments arranged by size:
2349 929 828 317 256 165 66 64
50 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5036
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
AvaI C'yCGr_G
Cuts at: 2056 2056
Size: 5036
BamHI G'GATC_C
Cuts at: 2061 2061
Size: 5036
BanI G'GyrC_C
Cuts at: 1724 2052 2466 2714 2749 1724
Size: 328 414 248 35 4011
Fragments arranged by size:
4011 414 328 248 35
BanII G_rGCy'C
Cuts at: 2050 3080 2050
Size: 1030 4006
BbeI G_GCGC'C
Cuts at: 2470 2718 2470
Size: 248 4788
BbsI GAAGACnn'nnnn_
Cuts at: 366 866 366
Size: 500 4536
BbvCI CC'TCA_GC
Cuts at: 1144 1144
Size: 5036
BciVI GTATCCnnnnn_n'
Cuts at: 140 795 2934 3990 5036 140
Size: 655 2139 1056 1046 140
Fragments arranged by size:
2139 1056 1046 655 140
BclI T'GATC_A
Cuts at: 447 2556 447
Size: 2109 2927
BglII A'GATC_T
Cuts at: 2551 4588 2551
Size: 2037 2999
BlpI GC'TnA_GC
Cuts at: 2148 2148
Size: 5036
Bme1580I G_kGCm'C
Cuts at: 1463 2661 2754 3878 1463
Size: 1198 93 1124 2621
Fragments arranged by size:
2621 1198 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1034 1083 1959 2248 2329 2664 4808 1034
Size: 49 876 289 81 335 2144 1262
Fragments arranged by size:
2144 1262 876 335 289 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3403 3403
Size: 5036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1844 1844
Size: 5036
Bpu10I CC'TnA_GC
Cuts at: 1144 1144
Size: 5036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 2212 3091 3805 4103 219
Size: 1993 879 714 298 1152
Fragments arranged by size:
1993 1152 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 611 4740 611
Size: 4129 907
BsaAI yAC'GTr
Cuts at: 610 1582 2389 610
Size: 972 807 3257
Fragments arranged by size:
3257 972 807
BsaBI GATnn'nnATC
Cuts at: 6 2550 4587 6
Size: 2544 2037 455
Fragments arranged by size:
2544 2037 455
BsaHI Gr'CG_yC
Cuts at: 2467 2715 3417 4913 2467
Size: 248 702 1496 2590
Fragments arranged by size:
2590 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1921 2225 2746 3424 3835 3982 4256 1921
Size: 304 521 678 411 147 274 2701
Fragments arranged by size:
2701 678 521 411 304 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 973 973
Size: 5036
BseYI C'CCAG_C
Cuts at: 117 1079 1265 1498 1769 2461 3219 3884 4292
Size: 962 186 233 271 692 758 665 408
Cuts at: 4292 117
Size: 861
Fragments arranged by size:
962 861 758 692 665 408 271 233
186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1670 1670
Size: 5036
BsiEI CG_ry'CG
Cuts at: 104 2578 2624 3854 104
Size: 2474 46 1230 1286
Fragments arranged by size:
2474 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2050 2828 3018 3878 2050
Size: 778 190 860 3208
Fragments arranged by size:
3208 860 778 190
BsmI GAATG_Cn'
Cuts at: 265 1100 265
Size: 835 4201
Bsp1286I G_dGCh'C
Cuts at: 1463 2050 2661 2754 2828 3018 3080 3878 1463
Size: 587 611 93 74 190 62 798 2621
Fragments arranged by size:
2621 798 611 587 190 93 74 62
BspHI T'CATG_A
Cuts at: 450 825 1590 3468 450
Size: 375 765 1878 2018
Fragments arranged by size:
2018 1878 765 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 2086 2300 2602 2983 1081
Size: 1005 214 302 381 3134
Fragments arranged by size:
3134 1005 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3328 3382 4866 5031 50
Size: 3278 54 1484 165 55
Fragments arranged by size:
3278 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2948 1046
Size: 1902 3134
BsrFI r'CCGG_y
Cuts at: 346 3034 3215 346
Size: 2688 181 2167
Fragments arranged by size:
2688 2167 181
BsrGI T'GTAC_A
Cuts at: 1866 1866
Size: 5036
BssSI C'ACGA_G
Cuts at: 1421 3307 4015 1421
Size: 1886 708 2442
Fragments arranged by size:
2442 1886 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1576 2413 1576
Size: 837 4199
BstBI TT'CG_AA
Cuts at: 3397 3397
Size: 5036
BstYI r'GATC_y
Cuts at: 1 2061 2528 2551 2886 3132 3438 3450 3536
Size: 2060 467 23 335 246 306 12 86
Cuts at: 3536 4588 1
Size: 1052 449
Fragments arranged by size:
2060 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 384 384
Size: 5036
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2986 3167 3330 2986
Size: 181 163 4692
Fragments arranged by size:
4692 181 163
BtsI GCAGTG_nn'
Cuts at: 571 986 1658 1768 2279 571
Size: 415 672 110 511 3328
Fragments arranged by size:
3328 672 511 415 110
DraI TTT'AAA
Cuts at: 2035 2035
Size: 5036
DraIII CAC_nnn'GTG
Cuts at: 254 1953 254
Size: 1699 3337
DrdI GACnn_nn'nnGTC
Cuts at: 2742 4086 2742
Size: 1344 3692
EaeI y'GGCC_r
Cuts at: 1784 2621 2795 3186 3213 4558 1784
Size: 837 174 391 27 1345 2262
Fragments arranged by size:
2262 1345 837 391 174 27
EagI C'GGCC_G
Cuts at: 2621 2621
Size: 5036
EarI CTCTTCn'nnn_
Cuts at: 291 407 3059 3269 291
Size: 116 2652 210 2058
Fragments arranged by size:
2652 2058 210 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3244 3986 4132 4846 3244
Size: 742 146 714 3434
Fragments arranged by size:
3434 742 714 146
EcoICRI GAG'CTC
Cuts at: 2048 2048
Size: 5036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1844 2861 3293 3646 4308 1189
Size: 655 1017 432 353 662 1917
Fragments arranged by size:
1917 1017 662 655 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1161 1624 2175 1161
Size: 463 551 4022
Fragments arranged by size:
4022 551 463
EcoRI G'AATT_C
Cuts at: 832 882 2040 832
Size: 50 1158 3828
Fragments arranged by size:
3828 1158 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 883 3055 883
Size: 2172 2864
FspI TGC'GCA
Cuts at: 2817 2817
Size: 5036
HaeII r_GCGC'y
Cuts at: 1266 2470 2718 3948 4719 1266
Size: 1204 248 1230 771 1583
Fragments arranged by size:
1583 1230 1204 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 2878 2908 2910 2940 138
Size: 32 2708 30 2 30 2234
Fragments arranged by size:
2708 2234 32 30 30 2
HincII GTy'rAC
Cuts at: 909 2075 909
Size: 1166 3870
KasI G'GCGC_C
Cuts at: 2466 2714 2466
Size: 248 4788
KpnI G_GTAC'C
Cuts at: 1728 2056 1728
Size: 328 4708
MfeI C'AATT_G
Cuts at: 985 985
Size: 5036
MluI A'CGCG_T
Cuts at: 4469 4469
Size: 5036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3795 3979 3795
Size: 184 4852
MscI TGG'CCA
Cuts at: 1786 2797 1786
Size: 1011 4025
MslI CAynn'nnrTG
Cuts at: 341 702 1862 3025 3152 341
Size: 361 1160 1163 127 2225
Fragments arranged by size:
2225 1163 1160 361 127
MspA1I CmG'CkG
Cuts at: 2147 2461 2821 3605 3850 2147
Size: 314 360 784 245 3333
Fragments arranged by size:
3333 784 360 314 245
NaeI GCC'GGC
Cuts at: 3217 3217
Size: 5036
NarI GG'CG_CC
Cuts at: 2467 2715 2467
Size: 248 4788
NcoI C'CATG_G
Cuts at: 384 384
Size: 5036
NdeI CA'TA_TG
Cuts at: 699 1127 1599 699
Size: 428 472 4136
Fragments arranged by size:
4136 472 428
NgoMIV G'CCGG_C
Cuts at: 3215 3215
Size: 5036
NspI r_CATG'y
Cuts at: 340 679 2089 2589 4192 340
Size: 339 1410 500 1603 1184
Fragments arranged by size:
1603 1410 1184 500 339
PciI A'CATG_T
Cuts at: 336 4188 336
Size: 3852 1184
PmeI GTTT'AAAC
Cuts at: 2035 2035
Size: 5036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 5004
PpuMI rG'GwC_Cy
Cuts at: 1161 1624 1161
Size: 463 4573
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1900 1932 1900
Size: 32 5004
PstI C_TGCA'G
Cuts at: 1092 2083 2768 1092
Size: 991 685 3360
Fragments arranged by size:
3360 991 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5036
PvuII CAG'CTG
Cuts at: 2461 2821 2461
Size: 360 4676
RsrII CG'GwC_CG
Cuts at: 3231 3231
Size: 5036
SacI G_AGCT'C
Cuts at: 2050 2050
Size: 5036
SalI G'TCGA_C
Cuts at: 2073 2073
Size: 5036
SapI GCTCTTCn'nnn_
Cuts at: 3059 3269 3059
Size: 210 4826
SbfI CC_TGCA'GG
Cuts at: 2083 2083
Size: 5036
ScaI AGT'ACT
Cuts at: 4553 4553
Size: 5036
SfcI C'TryA_G
Cuts at: 33 690 1088 1394 2079 2764 3732 3923 33
Size: 657 398 306 685 685 968 191 1146
Fragments arranged by size:
1146 968 685 685 657 398 306 191
SfoI GGC'GCC
Cuts at: 2468 2716 2468
Size: 248 4788
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5036
SmaI CCC'GGG
Cuts at: 2058 2058
Size: 5036
SmlI C'TyrA_G
Cuts at: 198 2191 3106 3820 4082 198
Size: 1993 915 714 262 1152
Fragments arranged by size:
1993 1152 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5036
SphI G_CATG'C
Cuts at: 2089 2589 2089
Size: 500 4536
SspI AAT'ATT
Cuts at: 1903 1903
Size: 5036
StuI AGG'CCT
Cuts at: 460 929 460
Size: 469 4567
StyI C'CwwG_G
Cuts at: 384 461 1033 2170 2524 384
Size: 77 572 1137 354 2896
Fragments arranged by size:
2896 1137 572 354 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 692 766 1941 2619 2752 3211 3420 692
Size: 74 1175 678 133 459 209 2308
Fragments arranged by size:
2308 1175 678 459 209 133 74
TatI w'GTAC_w
Cuts at: 1866 4551 1866
Size: 2685 2351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1731 1763 4553 4585 1731
Size: 32 2790 32 2182
Fragments arranged by size:
2790 2182 32 32
Tth111I GACn'n_nGTC
Cuts at: 2833 2833
Size: 5036
XbaI T'CTAG_A
Cuts at: 2067 2067
Size: 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 2531
Size: 5036
XmaI C'CCGG_G
Cuts at: 2056 2056
Size: 5036
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmBI BspEI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI