Restriction map for
pF1KB5585
(Circular) (Six-base) MAPSORT of: pF1KB5585.seq Check: 2547 from: 1 to: 4088
pF1KB5585 4088 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 1104 1104
Size: 4088
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 2698 3360 1913
Size: 432 353 662 2641
Fragments arranged by size:
2641 662 432 353
AflIII A'CryG_T
Cuts at: 929 1440 3240 3521 929
Size: 511 1800 281 1496
Fragments arranged by size:
1800 1496 511 281
AlwNI CAG_nnn'CTG
Cuts at: 279 1393 2831 279
Size: 1114 1438 1536
Fragments arranged by size:
1536 1438 1114
ApaLI G'TGCA_C
Cuts at: 2926 2926
Size: 4088
ApoI r'AATT_y
Cuts at: 136 498 871 1092 3441 3697 136
Size: 362 373 221 2349 256 527
Fragments arranged by size:
2349 527 373 362 256 221
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 1108 1108
Size: 4088
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 1104 1518 1766 1801 1104
Size: 414 248 35 3391
Fragments arranged by size:
3391 414 248 35
BanII G_rGCy'C
Cuts at: 1102 2132 1102
Size: 1030 3058
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BbsI GAAGACnn'nnnn_
Cuts at: 407 407
Size: 4088
BciVI GTATCCnnnnn_n'
Cuts at: 1986 3042 4088 1986
Size: 1056 1046 1986
Fragments arranged by size:
1986 1056 1046
BclI T'GATC_A
Cuts at: 467 1608 467
Size: 1141 2947
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 1200 1200
Size: 4088
Bme1580I G_kGCm'C
Cuts at: 1713 1806 2930 1713
Size: 93 1124 2871
Fragments arranged by size:
2871 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 1300 1381 1716 3860 267
Size: 1033 81 335 2144 495
Fragments arranged by size:
2144 1033 495 335 81
BmtI G_CTAG'C
Cuts at: 674 674
Size: 4088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2857 3155 1264
Size: 879 714 298 2197
Fragments arranged by size:
2197 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3792 3792
Size: 4088
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 214 1602 3639 6
Size: 208 1388 2037 455
Fragments arranged by size:
2037 1388 455 208
BsaHI Gr'CG_yC
Cuts at: 549 1519 1767 2469 3965 549
Size: 970 248 702 1496 672
Fragments arranged by size:
1496 970 702 672 248
BsaWI w'CCGG_w
Cuts at: 1277 1798 2476 2887 3034 3308 1277
Size: 521 678 411 147 274 2057
Fragments arranged by size:
2057 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1513 2271 2936 3344 1513
Size: 758 665 408 2257
Fragments arranged by size:
2257 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2906 104
Size: 1526 46 1230 1286
Fragments arranged by size:
1526 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1880 2070 2930 1102
Size: 778 190 860 2260
Fragments arranged by size:
2260 860 778 190
BsmI GAATG_Cn'
Cuts at: 953 953
Size: 4088
Bsp1286I G_dGCh'C
Cuts at: 1102 1713 1806 1880 2070 2132 2930 1102
Size: 611 93 74 190 62 798 2260
Fragments arranged by size:
2260 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2520 2520
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1138 1352 1654 2035 1138
Size: 214 302 381 3191
Fragments arranged by size:
3191 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2380 2434 3918 4083 50
Size: 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 2086 2267 2086
Size: 181 3907
BssSI C'ACGA_G
Cuts at: 2359 3067 2359
Size: 708 3380
BstAPI GCAn_nnn'nTGC
Cuts at: 874 1465 874
Size: 591 3497
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstYI r'GATC_y
Cuts at: 1 378 1113 1580 1603 1938 2184 2490 2502
Size: 377 735 467 23 335 246 306 12
Cuts at: 2502 2588 3640 1
Size: 86 1052 449
Fragments arranged by size:
1052 735 467 449 377 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 766 107
Size: 659 3429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 892 2038 2219 2382 892
Size: 1146 181 163 2598
Fragments arranged by size:
2598 1146 181 163
BtsI GCAGTG_nn'
Cuts at: 245 656 1331 245
Size: 411 675 3002
Fragments arranged by size:
3002 675 411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 822 857 822
Size: 35 4053
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 3138 1794
Size: 1344 2744
EaeI y'GGCC_r
Cuts at: 587 1673 1847 2238 2265 3610 587
Size: 1086 174 391 27 1345 1065
Fragments arranged by size:
1345 1086 1065 391 174 27
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 412 1044 2111 2321 412
Size: 632 1067 210 2179
Fragments arranged by size:
2179 1067 632 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 3038 3184 3898 2296
Size: 742 146 714 2486
Fragments arranged by size:
2486 742 714 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 2345 2698 3360 1913
Size: 432 353 662 2641
Fragments arranged by size:
2641 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 388 965 1227 388
Size: 577 262 3249
Fragments arranged by size:
3249 577 262
EcoRI G'AATT_C
Cuts at: 136 1092 136
Size: 956 3132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 4088
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 1522 1770 3000 3771 1522
Size: 248 1230 771 1839
Fragments arranged by size:
1839 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 706 738 1930 1960 1962 1992 380
Size: 32 294 32 1192 30 2 30 2476
Fragments arranged by size:
2476 1192 294 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 1108 1108
Size: 4088
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 126 2847 3031 126
Size: 2721 184 1183
Fragments arranged by size:
2721 1183 184
MscI TGG'CCA
Cuts at: 589 1849 589
Size: 1260 2828
MslI CAynn'nnrTG
Cuts at: 980 2077 2204 980
Size: 1097 127 2864
Fragments arranged by size:
2864 1097 127
MspA1I CmG'CkG
Cuts at: 122 1199 1513 1873 2657 2902 122
Size: 1077 314 360 784 245 1308
Fragments arranged by size:
1308 1077 784 360 314 245
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 107 766 107
Size: 659 3429
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NheI G'CTAG_C
Cuts at: 670 670
Size: 4088
NspI r_CATG'y
Cuts at: 933 1141 1641 3244 933
Size: 208 500 1603 1777
Fragments arranged by size:
1777 1603 500 208
PciI A'CATG_T
Cuts at: 929 3240 929
Size: 2311 1777
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 4056
PpuMI rG'GwC_Cy
Cuts at: 388 965 388
Size: 577 3511
PstI C_TGCA'G
Cuts at: 870 1135 1820 870
Size: 265 685 3138
Fragments arranged by size:
3138 685 265
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 1513 1873 1513
Size: 360 3728
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 866 1131 1816 2784 2975 33
Size: 833 265 685 968 191 1146
Fragments arranged by size:
1146 968 833 685 265 191
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 1243 2158 2872 3134 1243
Size: 915 714 262 2197
Fragments arranged by size:
2197 915 714 262
SpeI A'CTAG_T
Cuts at: 67 369 67
Size: 302 3786
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StyI C'CwwG_G
Cuts at: 107 128 766 1222 1576 107
Size: 21 638 456 354 2619
Fragments arranged by size:
2619 638 456 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1671 1804 2263 2472 1671
Size: 133 459 209 3287
Fragments arranged by size:
3287 459 209 133
TatI w'GTAC_w
Cuts at: 366 444 596 881 3603 366
Size: 78 152 285 2722 851
Fragments arranged by size:
2722 851 285 152 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4056
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI