Restriction map for
pF1KB5588
(Circular) (Six-base) MAPSORT of: pF1KB5588.seq Check: 3242 from: 1 to: 5288
pF1KB5588 5288 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 714 1601 714
Size: 887 4401
AccI GT'mk_AC
Cuts at: 635 666 2185 2326 635
Size: 31 1519 141 3597
Fragments arranged by size:
3597 1519 141 31
Acc65I G'GTAC_C
Cuts at: 2304 2304
Size: 5288
AclI AA'CG_TT
Cuts at: 5128 5128
Size: 5288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 864 1888 1943 2276 3113 3545 3898 4560
Size: 86 1024 55 333 837 432 353 662
Cuts at: 4560 778
Size: 1506
Fragments arranged by size:
1506 1024 837 662 432 353 333 86
55
AfeI AGC'GCT
Cuts at: 619 619
Size: 5288
AflIII A'CryG_T
Cuts at: 2640 4440 4721 2640
Size: 1800 281 3207
Fragments arranged by size:
3207 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1752 1938 1752
Size: 186 5102
AleI CACnn'nnGTG
Cuts at: 1712 1712
Size: 5288
AlwNI CAG_nnn'CTG
Cuts at: 2593 4031 2593
Size: 1438 3850
ApaI G_GGCC'C
Cuts at: 2271 2271
Size: 5288
ApaLI G'TGCA_C
Cuts at: 4126 4126
Size: 5288
ApoI r'AATT_y
Cuts at: 2292 4641 4897 2292
Size: 2349 256 2683
Fragments arranged by size:
2683 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
AvaI C'yCGr_G
Cuts at: 164 1097 1187 1475 2308 164
Size: 933 90 288 833 3144
Fragments arranged by size:
3144 933 833 288 90
AvrII C'CTAG_G
Cuts at: 1005 1005
Size: 5288
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1425 1458 1425
Size: 33 5255
BamHI G'GATC_C
Cuts at: 2313 2313
Size: 5288
BanI G'GyrC_C
Cuts at: 1997 2304 2718 2966 3001 1997
Size: 307 414 248 35 4284
Fragments arranged by size:
4284 414 307 248 35
BanII G_rGCy'C
Cuts at: 632 1186 1475 2096 2271 2302 3332 632
Size: 554 289 621 175 31 1030 2588
Fragments arranged by size:
2588 1030 621 554 289 175 31
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5040
BbsI GAAGACnn'nnnn_
Cuts at: 1529 1755 1529
Size: 226 5062
BbvCI CC'TCA_GC
Cuts at: 1984 1984
Size: 5288
BciVI GTATCCnnnnn_n'
Cuts at: 1426 3186 4242 5288 1426
Size: 1760 1056 1046 1426
Fragments arranged by size:
1760 1426 1056 1046
BclI T'GATC_A
Cuts at: 497 1386 2808 497
Size: 889 1422 2977
Fragments arranged by size:
2977 1422 889
BglI GCCn_nnn'nGGC
Cuts at: 140 2119 140
Size: 1979 3309
BglII A'GATC_T
Cuts at: 120 2803 4840 120
Size: 2683 2037 568
Fragments arranged by size:
2683 2037 568
BlpI GC'TnA_GC
Cuts at: 2400 2400
Size: 5288
Bme1580I G_kGCm'C
Cuts at: 985 2271 2913 3006 4130 985
Size: 1286 642 93 1124 2143
Fragments arranged by size:
2143 1286 1124 642 93
BmgBI CAC'GTC
Cuts at: 660 1269 660
Size: 609 4679
BmrI ACTGGGnnnn_n'
Cuts at: 1433 2500 2581 2916 5060 1433
Size: 1067 81 335 2144 1661
Fragments arranged by size:
2144 1661 1067 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 811 1268 1792 1819 2062 2173 303
Size: 508 457 524 27 243 111 3418
Fragments arranged by size:
3418 524 508 457 243 111 27
Bpu10I CC'TnA_GC
Cuts at: 1469 1984 1469
Size: 515 4773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1176 2464 3343 4057 4355 1176
Size: 1288 879 714 298 2109
Fragments arranged by size:
2109 1288 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1373 4992 1373
Size: 3619 1669
BsaAI yAC'GTr
Cuts at: 609 2641 609
Size: 2032 3256
BsaBI GATnn'nnATC
Cuts at: 6 745 2802 4839 6
Size: 739 2057 2037 455
Fragments arranged by size:
2057 2037 739 455
BsaHI Gr'CG_yC
Cuts at: 868 2719 2967 3669 5165 868
Size: 1851 248 702 1496 991
Fragments arranged by size:
1851 1496 991 702 248
BsaWI w'CCGG_w
Cuts at: 548 1052 2477 2998 3676 4087 4234 4508 548
Size: 504 1425 521 678 411 147 274 1328
Fragments arranged by size:
1425 1328 678 521 504 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1208 1238 1208
Size: 30 5258
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 542 709 1561 1569 1753 2293 542
Size: 167 852 8 184 540 3537
Fragments arranged by size:
3537 852 540 184 167 8
BseYI C'CCAG_C
Cuts at: 581 2713 3471 4136 4544 581
Size: 2132 758 665 408 1325
Fragments arranged by size:
2132 1325 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2005 2005
Size: 5288
BsiEI CG_ry'CG
Cuts at: 104 525 1610 2830 2876 4106 104
Size: 421 1085 1220 46 1230 1286
Fragments arranged by size:
1286 1230 1220 1085 421 46
BsiHKAI G_wGCw'C
Cuts at: 797 1778 2302 3080 3270 4130 797
Size: 981 524 778 190 860 1955
Fragments arranged by size:
1955 981 860 778 524 190
BsmI GAATG_Cn'
Cuts at: 2087 2087
Size: 5288
BsmBI CGTCTCn'nnnn_
Cuts at: 970 989 1244 970
Size: 19 255 5014
Fragments arranged by size:
5014 255 19
Bsp1286I G_dGCh'C
Cuts at: 632 797 985 1186 1475 1778 2096 2271 2302
Size: 165 188 201 289 303 318 175 31
Cuts at: 2302 2913 3006 3080 3270 3332 4130 632
Size: 611 93 74 190 62 798 1790
Fragments arranged by size:
1790 798 611 318 303 289 201 190
188 175 165 93 74 62 31
BspEI T'CCGG_A
Cuts at: 548 548
Size: 5288
BspHI T'CATG_A
Cuts at: 3720 3720
Size: 5288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 714 1601 2338 2552 2854 3235 714
Size: 887 737 214 302 381 2767
Fragments arranged by size:
2767 887 737 381 302 214
BsrBI CCG'CTC
Cuts at: 50 309 3580 3634 5118 5283 50
Size: 259 3271 54 1484 165 55
Fragments arranged by size:
3271 1484 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 3200 539
Size: 2661 2627
BsrFI r'CCGG_y
Cuts at: 1211 3286 3467 1211
Size: 2075 181 3032
Fragments arranged by size:
3032 2075 181
BssSI C'ACGA_G
Cuts at: 1708 3559 4267 1708
Size: 1851 708 2729
Fragments arranged by size:
2729 1851 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2665 2665
Size: 5288
BstBI TT'CG_AA
Cuts at: 3649 3649
Size: 5288
BstXI CCAn_nnnn'nTGG
Cuts at: 609 726 940 1714 609
Size: 117 214 774 4183
Fragments arranged by size:
4183 774 214 117
BstYI r'GATC_y
Cuts at: 1 120 945 965 2313 2780 2803 3138 3384
Size: 119 825 20 1348 467 23 335 246
Cuts at: 3384 3690 3702 3788 4840 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1348 1052 825 467 449 335 306 246
119 86 23 20 12
BstZ17I GTA'TAC
Cuts at: 2186 2186
Size: 5288
BtgI C'CryG_G
Cuts at: 107 1014 1610 1694 107
Size: 907 596 84 3701
Fragments arranged by size:
3701 907 596 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 863 2221 3238 3419 3582 863
Size: 1358 1017 181 163 2569
Fragments arranged by size:
2569 1358 1017 181 163
BtsI GCAGTG_nn'
Cuts at: 592 1118 2033 2531 592
Size: 526 915 498 3349
Fragments arranged by size:
3349 915 526 498
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1321 1356 1321
Size: 35 5253
DraI TTT'AAA
Cuts at: 2287 2287
Size: 5288
DrdI GACnn_nn'nnGTC
Cuts at: 2994 4338 2994
Size: 1344 3944
EaeI y'GGCC_r
Cuts at: 153 171 522 906 1213 1304 1342 1485 1607
Size: 18 351 384 307 91 38 143 122
Cuts at: 1607 1691 1974 2111 2231 2873 3047 3438 3465
Size: 84 283 137 120 642 174 391 27
Cuts at: 3465 4810 153
Size: 1345 631
Fragments arranged by size:
1345 642 631 391 384 351 307 283
174 143 137 122 120 91 84 38
27 18
EagI C'GGCC_G
Cuts at: 522 1607 2873 522
Size: 1085 1266 2937
Fragments arranged by size:
2937 1266 1085
EarI CTCTTCn'nnn_
Cuts at: 787 1033 1874 2252 3311 3521 787
Size: 246 841 378 1059 210 2554
Fragments arranged by size:
2554 1059 841 378 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 121 850 3496 4238 4384 5098 121
Size: 729 2646 742 146 714 311
Fragments arranged by size:
2646 742 729 714 311 146
EcoICRI GAG'CTC
Cuts at: 2300 2300
Size: 5288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 778 811 864 1268 1792 1819 1888 1943
Size: 475 33 53 404 524 27 69 55
Cuts at: 1943 2062 2173 2276 3113 3545 3898 4560 303
Size: 119 111 103 837 432 353 662 1031
Fragments arranged by size:
1031 837 662 524 475 432 404 353
119 111 103 69 55 53 33 27
EcoO109I rG'GnC_Cy
Cuts at: 326 1196 2016 2160 2427 326
Size: 870 820 144 267 3187
Fragments arranged by size:
3187 870 820 267 144
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 798 1170 3307 798
Size: 372 2137 2779
Fragments arranged by size:
2779 2137 372
FspI TGC'GCA
Cuts at: 3069 3069
Size: 5288
HaeII r_GCGC'y
Cuts at: 621 1027 1722 2722 2970 4200 4971 621
Size: 406 695 1000 248 1230 771 938
Fragments arranged by size:
1230 1000 938 771 695 406 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 495 527 1738 1770 1924 1956 3130
Size: 32 231 32 1211 32 154 32 1174
Cuts at: 3130 3160 3162 3192 232
Size: 30 2 30 2328
Fragments arranged by size:
2328 1211 1174 231 154 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2327 2327
Size: 5288
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5040
KpnI G_GTAC'C
Cuts at: 2308 2308
Size: 5288
MluI A'CGCG_T
Cuts at: 4721 4721
Size: 5288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 626 854 4047 4231 590
Size: 36 228 3193 184 1647
Fragments arranged by size:
3193 1647 228 184 36
MscI TGG'CCA
Cuts at: 173 1344 1487 1693 1976 2113 2233 3049 173
Size: 1171 143 206 283 137 120 816 2412
Fragments arranged by size:
2412 1171 816 283 206 143 137 120
MslI CAynn'nnrTG
Cuts at: 179 739 1712 3277 3404 179
Size: 560 973 1565 127 2063
Fragments arranged by size:
2063 1565 973 560 127
MspA1I CmG'CkG
Cuts at: 323 915 1016 1430 1921 2399 2713 3073 3857
Size: 592 101 414 491 478 314 360 784
Cuts at: 3857 4102 323
Size: 245 1509
Fragments arranged by size:
1509 784 592 491 478 414 360 314
245 101
NaeI GCC'GGC
Cuts at: 1213 3469 1213
Size: 2256 3032
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5040
NcoI C'CATG_G
Cuts at: 107 1694 107
Size: 1587 3701
NgoMIV G'CCGG_C
Cuts at: 1211 3467 1211
Size: 2256 3032
NspI r_CATG'y
Cuts at: 393 580 2341 2841 4444 393
Size: 187 1761 500 1603 1237
Fragments arranged by size:
1761 1603 1237 500 187
PasI CC'CwG_GG
Cuts at: 401 401
Size: 5288
PciI A'CATG_T
Cuts at: 4440 4440
Size: 5288
PflMI CCAn_nnn'nTGG
Cuts at: 686 686
Size: 5288
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2148 3734 3766 2116
Size: 32 1586 32 3638
Fragments arranged by size:
3638 1586 32 32
PpuMI rG'GwC_Cy
Cuts at: 326 1196 2160 326
Size: 870 964 3454
Fragments arranged by size:
3454 964 870
PshAI GACnn'nnGTC
Cuts at: 662 662
Size: 5288
PspOMI G'GGCC_C
Cuts at: 2267 2267
Size: 5288
PstI C_TGCA'G
Cuts at: 2335 3020 2335
Size: 685 4603
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5288
PvuII CAG'CTG
Cuts at: 915 2713 3073 915
Size: 1798 360 3130
Fragments arranged by size:
3130 1798 360
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5288
SacI G_AGCT'C
Cuts at: 2302 2302
Size: 5288
SacII CC_GC'GG
Cuts at: 1017 1017
Size: 5288
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5288
SanDI GG'GwC_CC
Cuts at: 326 326
Size: 5288
SapI GCTCTTCn'nnn_
Cuts at: 1033 3311 3521 1033
Size: 2278 210 2800
Fragments arranged by size:
2800 2278 210
SbfI CC_TGCA'GG
Cuts at: 2335 2335
Size: 5288
ScaI AGT'ACT
Cuts at: 283 2242 4805 283
Size: 1959 2563 766
Fragments arranged by size:
2563 1959 766
SfcI C'TryA_G
Cuts at: 33 285 667 2331 3016 3984 4175 33
Size: 252 382 1664 685 968 191 1146
Fragments arranged by size:
1664 1146 968 685 382 252 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2119 2119
Size: 5288
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5040
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
SmaI CCC'GGG
Cuts at: 1099 2310 1099
Size: 1211 4077
SmlI C'TyrA_G
Cuts at: 1155 2443 3358 4072 4334 1155
Size: 1288 915 714 262 2109
Fragments arranged by size:
2109 1288 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5288
SphI G_CATG'C
Cuts at: 580 2341 2841 580
Size: 1761 500 3027
Fragments arranged by size:
3027 1761 500
StuI AGG'CCT
Cuts at: 877 2263 877
Size: 1386 3902
StyI C'CwwG_G
Cuts at: 107 269 1005 1694 2422 2776 107
Size: 162 736 689 728 354 2619
Fragments arranged by size:
2619 736 728 689 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2871 3004 3463 3672 2871
Size: 133 459 209 4487
Fragments arranged by size:
4487 459 209 133
TatI w'GTAC_w
Cuts at: 281 647 812 821 1826 2240 4803 281
Size: 366 165 9 1005 414 2563 766
Fragments arranged by size:
2563 1005 766 414 366 165 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1208 1240 4805 4837 1208
Size: 32 3565 32 1659
Fragments arranged by size:
3565 1659 32 32
Tth111I GACn'n_nGTC
Cuts at: 3085 3085
Size: 5288
XbaI T'CTAG_A
Cuts at: 1392 2319 1392
Size: 927 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1812 1950 2783 1812
Size: 138 833 4317
Fragments arranged by size:
4317 833 138
XmaI C'CCGG_G
Cuts at: 1097 2308 1097
Size: 1211 4077
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI BcgI BfrBI
BmtI BsiWI BsrGI BssHII BstEII Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PsiI
PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI