Restriction map for
pF1KB5590
(Circular) (Six-base) MAPSORT of: pF1KB5590.seq Check: 195 from: 1 to: 4160
pF1KB5590 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2417 3164 3432 1985
Size: 432 747 268 2713
Fragments arranged by size:
2713 747 432 268
AflIII A'CryG_T
Cuts at: 665 1512 2616 3593 665
Size: 847 1104 977 1232
Fragments arranged by size:
1232 1104 977 847
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 717 749 717
Size: 32 4128
AlwNI CAG_nnn'CTG
Cuts at: 1465 3032 1465
Size: 1567 2593
ApaI G_GGCC'C
Cuts at: 130 130
Size: 4160
ApaLI G'TGCA_C
Cuts at: 309 390 477 2930 309
Size: 81 87 2453 1539
Fragments arranged by size:
2453 1539 87 81
ApoI r'AATT_y
Cuts at: 952 1164 3513 3769 952
Size: 212 2349 256 1343
Fragments arranged by size:
2349 1343 256 212
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 558 577 764 1180 558
Size: 19 187 416 3538
Fragments arranged by size:
3538 416 187 19
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 209 225 1176 1590 1838 1873 209
Size: 16 951 414 248 35 2496
Fragments arranged by size:
2496 951 414 248 35 16
BanII G_rGCy'C
Cuts at: 130 614 1174 2204 130
Size: 484 560 1030 2086
Fragments arranged by size:
2086 1030 560 484
BbeI G_GCGC'C
Cuts at: 229 1594 1842 229
Size: 1365 248 2547
Fragments arranged by size:
2547 1365 248
BbsI GAAGACnn'nnnn_
Cuts at: 668 943 668
Size: 275 3885
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 242 646 1034 242
Size: 404 388 3368
Fragments arranged by size:
3368 404 388
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 130 214 313 394 481 1030 1785 1878 2934
Size: 84 99 81 87 549 755 93 1056
Cuts at: 2934 130
Size: 1356
Fragments arranged by size:
1356 1056 755 549 99 93 87 84
81
BmrI ACTGGGnnnn_n'
Cuts at: 1372 1453 1788 3932 1372
Size: 81 335 2144 1600
Fragments arranged by size:
2144 1600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 482 1513 482
Size: 1031 3129
BsaBI GATnn'nnATC
Cuts at: 6 754 1063 1674 3711 6
Size: 748 309 611 2037 455
Fragments arranged by size:
2037 748 611 455 309
BsaHI Gr'CG_yC
Cuts at: 226 1591 1839 2541 4037 226
Size: 1365 248 702 1496 349
Fragments arranged by size:
1496 1365 702 349 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 586 616 717 747 769 799 1009
Size: 30 210 30 101 30 22 30 210
Cuts at: 1009 1039 346
Size: 30 3467
Fragments arranged by size:
3467 210 210 101 30 30 30 30
30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 576 607 145
Size: 431 31 3698
Fragments arranged by size:
3698 431 31
BseYI C'CCAG_C
Cuts at: 297 538 1585 2343 2920 3416 297
Size: 241 1047 758 577 496 1041
Fragments arranged by size:
1047 1041 758 577 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 909 969 537
Size: 372 60 3728
Fragments arranged by size:
3728 372 60
BsiEI CG_ry'CG
Cuts at: 104 175 179 978 1702 1748 2956 104
Size: 71 4 799 724 46 1208 1308
Fragments arranged by size:
1308 1208 799 724 71 46 4
BsiHKAI G_wGCw'C
Cuts at: 313 394 411 481 1174 1952 2142 2934 313
Size: 81 17 70 693 778 190 792 1539
Fragments arranged by size:
1539 792 778 693 190 81 70 17
Bsp1286I G_dGCh'C
Cuts at: 130 214 313 394 411 481 614 1030 1174
Size: 84 99 81 17 70 133 416 144
Cuts at: 1174 1785 1878 1952 2142 2204 2934 130
Size: 611 93 74 190 62 730 1356
Fragments arranged by size:
1356 730 611 416 190 144 133 99
93 84 81 74 70 62 17
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 1043 3336 1043
Size: 2293 1867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 170 174 206 222 1011 2158 2339 2548 170
Size: 4 32 16 789 1147 181 209 1782
Fragments arranged by size:
1782 1147 789 209 181 32 16 4
BssSI C'ACGA_G
Cuts at: 253 2431 2789 253
Size: 2178 358 1624
Fragments arranged by size:
2178 1624 358
BstAPI GCAn_nnn'nTGC
Cuts at: 872 1537 872
Size: 665 3495
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstEII G'GTnAC_C
Cuts at: 768 768
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 297 705 297
Size: 408 3752
BstYI r'GATC_y
Cuts at: 1 1185 1652 1675 2010 2256 3268 3354 3366
Size: 1184 467 23 335 246 1012 86 12
Cuts at: 3366 3712 1
Size: 346 449
Fragments arranged by size:
1184 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 626 626
Size: 4160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 726 761 726
Size: 35 4125
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DraIII CAC_nnn'GTG
Cuts at: 722 722
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1039 1866 2724 1039
Size: 827 858 2475
Fragments arranged by size:
2475 858 827
EaeI y'GGCC_r
Cuts at: 172 176 183 368 975 1745 1919 2310 2337
Size: 4 7 185 607 770 174 391 27
Cuts at: 2337 3682 172
Size: 1345 650
Fragments arranged by size:
1345 770 650 607 391 185 174 27
7 4
EagI C'GGCC_G
Cuts at: 172 176 975 1745 172
Size: 4 799 770 2587
Fragments arranged by size:
2587 799 770 4
EarI CTCTTCn'nnn_
Cuts at: 352 842 2183 2393 352
Size: 490 1341 210 2119
Fragments arranged by size:
2119 1341 490 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 2678 2824 3970 2368
Size: 310 146 1146 2558
Fragments arranged by size:
2558 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1985 2417 3164 3432 1985
Size: 432 747 268 2713
Fragments arranged by size:
2713 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 558 789 558
Size: 231 3929
EcoO109I rG'GnC_Cy
Cuts at: 127 342 1299 127
Size: 215 957 2988
Fragments arranged by size:
2988 957 215
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 363 2179 363
Size: 1816 2344
FseI GG_CCGG'CC
Cuts at: 178 178
Size: 4160
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 229 731 1594 1842 2864 3843 229
Size: 502 863 248 1022 979 546
Fragments arranged by size:
1022 979 863 546 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 769 801 2002 2032 2034 2064 346
Size: 32 391 32 1201 30 2 30 2442
Fragments arranged by size:
2442 1201 391 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 225 1590 1838 225
Size: 1365 248 2547
Fragments arranged by size:
2547 1365 248
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3015 2831
Size: 184 3976
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 931 2149 2276 931
Size: 1218 127 2815
Fragments arranged by size:
2815 1218 127
MspA1I CmG'CkG
Cuts at: 197 297 443 464 1271 1585 1945 2958 3203
Size: 100 146 21 807 314 360 1013 245
Cuts at: 3203 197
Size: 1154
Fragments arranged by size:
1154 1013 807 360 314 245 146 100
21
NaeI GCC'GGC
Cuts at: 172 176 224 2341 172
Size: 4 48 2117 1991
Fragments arranged by size:
2117 1991 48 4
NarI GG'CG_CC
Cuts at: 226 1591 1839 226
Size: 1365 248 2547
Fragments arranged by size:
2547 1365 248
NgoMIV G'CCGG_C
Cuts at: 170 174 222 2339 170
Size: 4 48 2117 1991
Fragments arranged by size:
2117 1991 48 4
NspI r_CATG'y
Cuts at: 510 1213 1713 2620 510
Size: 703 500 907 2050
Fragments arranged by size:
2050 907 703 500
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 627 722 627
Size: 95 4065
PfoI T'CCnGG_A
Cuts at: 1095 1095
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PmlI CAC'GTG
Cuts at: 482 482
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 717 749 3299 3331 717
Size: 32 2550 32 1546
Fragments arranged by size:
2550 1546 32 32
PshAI GACnn'nnGTC
Cuts at: 530 530
Size: 4160
PspOMI G'GGCC_C
Cuts at: 126 126
Size: 4160
PstI C_TGCA'G
Cuts at: 169 196 793 1207 1892 169
Size: 27 597 414 685 2437
Fragments arranged by size:
2437 685 597 414 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 297 464 1585 1945 297
Size: 167 1121 360 2512
Fragments arranged by size:
2512 1121 360 167
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 793 1207 793
Size: 414 3746
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 165 192 789 1128 1203 1888 2881 3072
Size: 132 27 597 339 75 685 993 191
Cuts at: 3072 33
Size: 1121
Fragments arranged by size:
1121 993 685 597 339 191 132 75
27
SfoI GGC'GCC
Cuts at: 227 1592 1840 227
Size: 1365 248 2547
Fragments arranged by size:
2547 1365 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 579 766 1182 579
Size: 187 416 3557
Fragments arranged by size:
3557 416 187
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 510 1213 1713 510
Size: 703 500 2957
Fragments arranged by size:
2957 703 500
StuI AGG'CCT
Cuts at: 828 828
Size: 4160
StyI C'CwwG_G
Cuts at: 614 1294 1648 614
Size: 680 354 3126
Fragments arranged by size:
3126 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 588 1743 1876 2335 2544 588
Size: 1155 133 459 209 2204
Fragments arranged by size:
2204 1155 459 209 133
TatI w'GTAC_w
Cuts at: 1124 3675 1124
Size: 2551 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 586 618 1009 1041 3677 3709 586
Size: 32 391 32 2636 32 1037
Fragments arranged by size:
2636 1037 391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 628 1011 1655 628
Size: 383 644 3133
Fragments arranged by size:
3133 644 383
XmaI C'CCGG_G
Cuts at: 577 764 1180 577
Size: 187 416 3557
Fragments arranged by size:
3557 416 187
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PpuMI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI