Restriction map for
pF1KB5591
(Circular) (Six-base) MAPSORT of: pF1KB5591.seq Check: 9567 from: 1 to: 5891
pF1KB5591 5891 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 2240 2289 702
Size: 1538 49 4304
Fragments arranged by size:
4304 1538 49
AccI GT'mk_AC
Cuts at: 1826 2929 1826
Size: 1103 4788
Acc65I G'GTAC_C
Cuts at: 2907 2907
Size: 5891
AclI AA'CG_TT
Cuts at: 5731 5731
Size: 5891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1555 3716 4148 4501 5163 566
Size: 989 2161 432 353 662 1294
Fragments arranged by size:
2161 1294 989 662 432 353
AflII C'TTAA_G
Cuts at: 2839 2839
Size: 5891
AflIII A'CryG_T
Cuts at: 768 3243 5043 5324 768
Size: 2475 1800 281 1335
Fragments arranged by size:
2475 1800 1335 281
AleI CACnn'nnGTG
Cuts at: 1648 1648
Size: 5891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2430
Size: 32 5859
AlwNI CAG_nnn'CTG
Cuts at: 544 681 2632 3196 4634 544
Size: 137 1951 564 1438 1801
Fragments arranged by size:
1951 1801 1438 564 137
ApaLI G'TGCA_C
Cuts at: 4729 4729
Size: 5891
ApoI r'AATT_y
Cuts at: 204 2160 2895 5244 5500 204
Size: 1956 735 2349 256 595
Fragments arranged by size:
2349 1956 735 595 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5891
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5891
AvaI C'yCGr_G
Cuts at: 453 1521 2059 2775 2911 453
Size: 1068 538 716 136 3433
Fragments arranged by size:
3433 1068 716 538 136
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1341 1374 1341
Size: 33 5858
BamHI G'GATC_C
Cuts at: 836 2679 2916 836
Size: 1843 237 3811
Fragments arranged by size:
3811 1843 237
BanI G'GyrC_C
Cuts at: 2635 2907 3321 3569 3604 2635
Size: 272 414 248 35 4922
Fragments arranged by size:
4922 414 272 248 35
BanII G_rGCy'C
Cuts at: 460 1379 1492 1626 2069 2713 2788 2828 2905
Size: 919 113 134 443 644 75 40 77
Cuts at: 2905 3935 460
Size: 1030 2416
Fragments arranged by size:
2416 1030 919 644 443 134 113 77
75 40
BbeI G_GCGC'C
Cuts at: 2639 3325 3573 2639
Size: 686 248 4957
Fragments arranged by size:
4957 686 248
BciVI GTATCCnnnnn_n'
Cuts at: 623 3789 4845 5891 623
Size: 3166 1056 1046 623
Fragments arranged by size:
3166 1056 1046 623
BclI T'GATC_A
Cuts at: 3411 3411
Size: 5891
BglI GCCn_nnn'nGGC
Cuts at: 1523 1700 1523
Size: 177 5714
BglII A'GATC_T
Cuts at: 527 1454 1578 3406 5443 527
Size: 927 124 1828 2037 975
Fragments arranged by size:
2037 1828 975 927 124
BlpI GC'TnA_GC
Cuts at: 3003 3003
Size: 5891
Bme1580I G_kGCm'C
Cuts at: 3516 3609 4733 3516
Size: 93 1124 4674
Fragments arranged by size:
4674 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1487 1719 2725 3103 3184 3519 5663 1487
Size: 232 1006 378 81 335 2144 1715
Fragments arranged by size:
2144 1715 1006 378 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4258 4258
Size: 5891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1078 1393 1506 1888 2109 2518 2622 2827
Size: 363 315 113 382 221 409 104 205
Cuts at: 2827 715
Size: 3779
Fragments arranged by size:
3779 409 382 363 315 221 205 113
104
Bpu10I CC'TnA_GC
Cuts at: 2782 2782
Size: 5891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3067 3946 4660 4958 3067
Size: 879 714 298 4000
Fragments arranged by size:
4000 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5595 5595
Size: 5891
BsaAI yAC'GTr
Cuts at: 3244 3244
Size: 5891
BsaBI GATnn'nnATC
Cuts at: 6 664 3405 5442 6
Size: 658 2741 2037 455
Fragments arranged by size:
2741 2037 658 455
BsaHI Gr'CG_yC
Cuts at: 2636 2720 3322 3570 4272 5768 2636
Size: 84 602 248 702 1496 2759
Fragments arranged by size:
2759 1496 702 602 248 84
BsaWI w'CCGG_w
Cuts at: 2102 2138 2449 3080 3601 4279 4690 4837 5111
Size: 36 311 631 521 678 411 147 274
Cuts at: 5111 2102
Size: 2882
Fragments arranged by size:
2882 678 631 521 411 311 274 147
36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 1386 1416 1479 1509 1825 1855 2248
Size: 30 1115 30 63 30 316 30 393
Cuts at: 2248 2278 2314 2344 241
Size: 30 36 30 3788
Fragments arranged by size:
3788 1115 393 316 63 36 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 441 1576 1646 1984 2077 441
Size: 1135 70 338 93 4255
Fragments arranged by size:
4255 1135 338 93 70
BseYI C'CCAG_C
Cuts at: 1419 1550 1736 1875 1917 2392 3316 4074 4739
Size: 131 186 139 42 475 924 758 665
Cuts at: 4739 5147 1419
Size: 408 2163
Fragments arranged by size:
2163 924 758 665 475 408 186 139
131 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 1517 2745 2762 2781 615
Size: 902 1228 17 19 3725
Fragments arranged by size:
3725 1228 902 19 17
BsiEI CG_ry'CG
Cuts at: 104 2139 2283 2648 3433 3479 4709 104
Size: 2035 144 365 785 46 1230 1286
Fragments arranged by size:
2035 1286 1230 785 365 144 46
BsiHKAI G_wGCw'C
Cuts at: 1379 2713 2788 2828 2905 3683 3873 4733 1379
Size: 1334 75 40 77 778 190 860 2537
Fragments arranged by size:
2537 1334 860 778 190 77 75 40
BsmI GAATG_Cn'
Cuts at: 761 761
Size: 5891
BsmBI CGTCTCn'nnnn_
Cuts at: 1906 1906
Size: 5891
Bsp1286I G_dGCh'C
Cuts at: 460 1379 1492 1626 2069 2713 2788 2828 2905
Size: 919 113 134 443 644 75 40 77
Cuts at: 2905 3516 3609 3683 3873 3935 4733 460
Size: 611 93 74 190 62 798 1618
Fragments arranged by size:
1618 919 798 644 611 443 190 134
113 93 77 75 74 62 40
BspEI T'CCGG_A
Cuts at: 2102 2449 2102
Size: 347 5544
BspHI T'CATG_A
Cuts at: 4323 4323
Size: 5891
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 1112 2240 2289 2941 3155 3457 3838 702
Size: 410 1128 49 652 214 302 381 2755
Fragments arranged by size:
2755 1128 652 410 381 302 214 49
BsrBI CCG'CTC
Cuts at: 50 1520 4183 4237 5721 5886 50
Size: 1470 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 1470 165 55 54
BsrDI GCAATG_nn'
Cuts at: 945 1439 3803 945
Size: 494 2364 3033
Fragments arranged by size:
3033 2364 494
BsrFI r'CCGG_y
Cuts at: 1034 1232 1845 3889 4070 1034
Size: 198 613 2044 181 2855
Fragments arranged by size:
2855 2044 613 198 181
BsrGI T'GTAC_A
Cuts at: 2669 2669
Size: 5891
BssSI C'ACGA_G
Cuts at: 4162 4870 4162
Size: 708 5183
BstAPI GCAn_nnn'nTGC
Cuts at: 2304 3268 2304
Size: 964 4927
BstBI TT'CG_AA
Cuts at: 1754 4252 1754
Size: 2498 3393
BstEII G'GTnAC_C
Cuts at: 238 238
Size: 5891
BstXI CCAn_nnnn'nTGG
Cuts at: 1419 1419
Size: 5891
BstYI r'GATC_y
Cuts at: 1 527 836 1454 1578 2679 2916 3383 3406
Size: 526 309 618 124 1101 237 467 23
Cuts at: 3406 3741 3987 4293 4305 4391 5443 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1101 1052 618 526 467 449 335 309
306 246 237 124 86 23 12
BstZ17I GTA'TAC
Cuts at: 1827 1827
Size: 5891
Bsu36I CC'TnA_GG
Cuts at: 1544 2625 1544
Size: 1081 4810
BtgI C'CryG_G
Cuts at: 107 1041 1626 1904 2273 2283 107
Size: 934 585 278 369 10 3715
Fragments arranged by size:
3715 934 585 369 278 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 414 1116 2685 3841 4022 4185 414
Size: 702 1569 1156 181 163 2120
Fragments arranged by size:
2120 1569 1156 702 181 163
BtsI GCAGTG_nn'
Cuts at: 1309 1643 2537 3134 1309
Size: 334 894 597 4066
Fragments arranged by size:
4066 894 597 334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 402 437 605 640 777 812 1265 1300 402
Size: 35 168 35 137 35 453 35 4993
Fragments arranged by size:
4993 453 168 137 35 35 35 35
DraI TTT'AAA
Cuts at: 2890 2890
Size: 5891
DraIII CAC_nnn'GTG
Cuts at: 165 1982 165
Size: 1817 4074
DrdI GACnn_nn'nnGTC
Cuts at: 3597 4941 3597
Size: 1344 4547
EaeI y'GGCC_r
Cuts at: 647 872 963 1127 2280 3476 3650 4041 4068
Size: 225 91 164 1153 1196 174 391 27
Cuts at: 4068 5413 647
Size: 1345 1125
Fragments arranged by size:
1345 1196 1153 1125 391 225 174 164
91 27
EagI C'GGCC_G
Cuts at: 2280 3476 2280
Size: 1196 4695
EarI CTCTTCn'nnn_
Cuts at: 1384 1470 1927 1934 1961 3914 4124 1384
Size: 86 457 7 27 1953 210 3151
Fragments arranged by size:
3151 1953 457 210 86 27 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1324 1449 2394 4099 4841 4987 5701 1324
Size: 125 945 1705 742 146 714 1514
Fragments arranged by size:
1705 1514 945 742 714 146 125
EcoICRI GAG'CTC
Cuts at: 1377 2711 2786 2826 2903 1377
Size: 1334 75 40 77 4365
Fragments arranged by size:
4365 1334 77 75 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 715 1078 1393 1506 1555 1888 2109 2518
Size: 149 363 315 113 49 333 221 409
Cuts at: 2518 2622 2827 3716 4148 4501 5163 566
Size: 104 205 889 432 353 662 1294
Fragments arranged by size:
1294 889 662 432 409 363 353 333
315 221 205 149 113 104 49
EcoO109I rG'GnC_Cy
Cuts at: 1725 1733 1921 3030 1725
Size: 8 188 1109 4586
Fragments arranged by size:
4586 1109 188 8
EcoRI G'AATT_C
Cuts at: 204 2895 204
Size: 2691 3200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 900 3910 381
Size: 519 3010 2362
Fragments arranged by size:
3010 2362 519
FspI TGC'GCA
Cuts at: 1672 3672 1672
Size: 2000 3891
HaeII r_GCGC'y
Cuts at: 2296 2639 3325 3573 4803 5574 2296
Size: 343 686 248 1230 771 2613
Fragments arranged by size:
2613 1230 771 686 343 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 379 411 871 903 1625 1657 1825
Size: 32 106 32 460 32 722 32 168
Cuts at: 1825 1857 3733 3763 3765 3795 241
Size: 32 1876 30 2 30 2337
Fragments arranged by size:
2337 1876 722 460 168 106 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2930 2930
Size: 5891
KasI G'GCGC_C
Cuts at: 2635 3321 3569 2635
Size: 686 248 4957
Fragments arranged by size:
4957 686 248
KpnI G_GTAC'C
Cuts at: 2911 2911
Size: 5891
MfeI C'AATT_G
Cuts at: 2040 2040
Size: 5891
MluI A'CGCG_T
Cuts at: 5324 5324
Size: 5891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 509 1103 1436 2056 4650 4834 509
Size: 594 333 620 2594 184 1566
Fragments arranged by size:
2594 1566 620 594 333 184
MscI TGG'CCA
Cuts at: 649 874 3652 649
Size: 225 2778 2888
Fragments arranged by size:
2888 2778 225
MslI CAynn'nnrTG
Cuts at: 112 862 868 1046 1648 2704 3880 4007 112
Size: 750 6 178 602 1056 1176 127 1996
Fragments arranged by size:
1996 1176 1056 750 602 178 127 6
MspA1I CmG'CkG
Cuts at: 1628 2285 3002 3316 3676 4460 4705 1628
Size: 657 717 314 360 784 245 2814
Fragments arranged by size:
2814 784 717 657 360 314 245
NaeI GCC'GGC
Cuts at: 1036 1847 4072 1036
Size: 811 2225 2855
Fragments arranged by size:
2855 2225 811
NarI GG'CG_CC
Cuts at: 2636 3322 3570 2636
Size: 686 248 4957
Fragments arranged by size:
4957 686 248
NcoI C'CATG_G
Cuts at: 107 1041 107
Size: 934 4957
NgoMIV G'CCGG_C
Cuts at: 1034 1845 4070 1034
Size: 811 2225 2855
Fragments arranged by size:
2855 2225 811
NotI GC'GGCC_GC
Cuts at: 2280 2280
Size: 5891
NspI r_CATG'y
Cuts at: 772 1419 1767 2944 3444 5047 772
Size: 647 348 1177 500 1603 1616
Fragments arranged by size:
1616 1603 1177 647 500 348
PciI A'CATG_T
Cuts at: 768 5043 768
Size: 4275 1616
PflMI CCAn_nnn'nTGG
Cuts at: 772 772
Size: 5891
PmeI GTTT'AAAC
Cuts at: 2890 2890
Size: 5891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4337 4369 4337
Size: 32 5859
PpuMI rG'GwC_Cy
Cuts at: 1725 1733 1921 1725
Size: 8 188 5695
Fragments arranged by size:
5695 188 8
PspXI vC'TCGA_Gb
Cuts at: 2059 2059
Size: 5891
PstI C_TGCA'G
Cuts at: 677 1999 2177 2300 2938 3623 677
Size: 1322 178 123 638 685 2945
Fragments arranged by size:
2945 1322 685 638 178 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5891
PvuII CAG'CTG
Cuts at: 3316 3676 3316
Size: 360 5531
RsrII CG'GwC_CG
Cuts at: 4086 4086
Size: 5891
SacI G_AGCT'C
Cuts at: 1379 2713 2788 2828 2905 1379
Size: 1334 75 40 77 4365
Fragments arranged by size:
4365 1334 77 75 40
SacII CC_GC'GG
Cuts at: 1629 2286 1629
Size: 657 5234
SalI G'TCGA_C
Cuts at: 2928 2928
Size: 5891
SanDI GG'GwC_CC
Cuts at: 1921 1921
Size: 5891
SapI GCTCTTCn'nnn_
Cuts at: 1384 1927 3914 4124 1384
Size: 543 1987 210 3151
Fragments arranged by size:
3151 1987 543 210
SbfI CC_TGCA'GG
Cuts at: 2938 2938
Size: 5891
ScaI AGT'ACT
Cuts at: 793 5408 793
Size: 4615 1276
SexAI A'CCwGG_T
Cuts at: 902 902
Size: 5891
SfcI C'TryA_G
Cuts at: 33 673 1689 1995 2173 2296 2934 3619 4587
Size: 640 1016 306 178 123 638 685 968
Cuts at: 4587 4778 33
Size: 191 1146
Fragments arranged by size:
1146 1016 968 685 640 638 306 191
178 123
SfoI GGC'GCC
Cuts at: 2637 3323 3571 2637
Size: 686 248 4957
Fragments arranged by size:
4957 686 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5891
SgrAI Cr'CCGG_yG
Cuts at: 1845 1845
Size: 5891
SmaI CCC'GGG
Cuts at: 2913 2913
Size: 5891
SmlI C'TyrA_G
Cuts at: 2059 2839 3046 3961 4675 4937 2059
Size: 780 207 915 714 262 3013
Fragments arranged by size:
3013 915 780 714 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5891
SphI G_CATG'C
Cuts at: 2944 3444 2944
Size: 500 5391
StuI AGG'CCT
Cuts at: 1436 2747 1436
Size: 1311 4580
StyI C'CwwG_G
Cuts at: 107 875 1041 3025 3379 107
Size: 768 166 1984 354 2619
Fragments arranged by size:
2619 1984 768 354 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1486 1605 2662 3474 3607 4066 4275 1486
Size: 119 1057 812 133 459 209 3102
Fragments arranged by size:
3102 1057 812 459 209 133 119
TatI w'GTAC_w
Cuts at: 791 2669 5406 791
Size: 1878 2737 1276
Fragments arranged by size:
2737 1878 1276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1386 1418 1479 1511 1913 1945 2664 2696 2705
Size: 32 61 32 402 32 719 32 9
Cuts at: 2705 2737 5408 5440 1386
Size: 32 2671 32 1837
Fragments arranged by size:
2671 1837 719 402 61 32 32 32
32 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 3688 3688
Size: 5891
XbaI T'CTAG_A
Cuts at: 2922 2922
Size: 5891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 2400 2712 3386 657
Size: 1743 312 674 3162
Fragments arranged by size:
3162 1743 674 312
XhoI C'TCGA_G
Cuts at: 2059 2059
Size: 5891
XmaI C'CCGG_G
Cuts at: 2911 2911
Size: 5891
XmnI GAAnn'nnTTC
Cuts at: 1718 1718
Size: 5891
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BssHII
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PsrI SfiI SnaBI SrfI SspI SwaI
ZraI