Restriction map for
pF1KB5593
(Circular) (Six-base) MAPSORT of: pF1KB5593.seq Check: 8402 from: 1 to: 8702
pF1KB5593 8702 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 637 637
Size: 8702
AccI GT'mk_AC
Cuts at: 641 3045 3829 5740 641
Size: 2404 784 1911 3603
Fragments arranged by size:
3603 2404 1911 784
Acc65I G'GTAC_C
Cuts at: 2421 5718 2421
Size: 3297 5405
AclI AA'CG_TT
Cuts at: 8542 8542
Size: 8702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1229 1367 2227 2360 4265 4642 4868 5171
Size: 540 138 860 133 1905 377 226 303
Cuts at: 5171 5373 6527 6959 7706 7974 689
Size: 202 1154 432 747 268 1417
Fragments arranged by size:
1905 1417 1154 860 747 540 432 377
303 268 226 202 138 133
AflII C'TTAA_G
Cuts at: 1527 1527
Size: 8702
AflIII A'CryG_T
Cuts at: 1087 6054 7158 8135 1087
Size: 4967 1104 977 1654
Fragments arranged by size:
4967 1654 1104 977
AgeI A'CCGG_T
Cuts at: 7090 7090
Size: 8702
AleI CACnn'nnGTG
Cuts at: 3962 3962
Size: 8702
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 711 1985 5561 711
Size: 1274 3576 3852
Fragments arranged by size:
3852 3576 1274
AlwNI CAG_nnn'CTG
Cuts at: 1179 4216 4936 4954 5185 5655 6007 7574 1179
Size: 3037 720 18 231 470 352 1567 2307
Fragments arranged by size:
3037 2307 1567 720 470 352 231 18
ApaI G_GGCC'C
Cuts at: 5420 5420
Size: 8702
ApaLI G'TGCA_C
Cuts at: 7472 7472
Size: 8702
ApoI r'AATT_y
Cuts at: 241 787 1015 2266 3882 5089 5706 8055 8311
Size: 546 228 1251 1616 1207 617 2349 256
Cuts at: 8311 241
Size: 632
Fragments arranged by size:
2349 1616 1251 1207 632 617 546 256
228
AseI AT'TA_AT
Cuts at: 20 152 3239 3281 20
Size: 132 3087 42 5441
Fragments arranged by size:
5441 3087 132 42
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8702
AvaI C'yCGr_G
Cuts at: 5609 5722 5609
Size: 113 8589
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5449 5482 5449
Size: 33 8669
BamHI G'GATC_C
Cuts at: 4047 5727 4047
Size: 1680 7022
BanI G'GyrC_C
Cuts at: 2421 5595 5718 6132 6380 6415 2421
Size: 3174 123 414 248 35 4708
Fragments arranged by size:
4708 3174 414 248 123 35
BanII G_rGCy'C
Cuts at: 3274 4691 4860 5420 5716 6746 3274
Size: 1417 169 560 296 1030 5230
Fragments arranged by size:
5230 1417 1030 560 296 169
BbeI G_GCGC'C
Cuts at: 6136 6384 6136
Size: 248 8454
BbsI GAAGACnn'nnnn_
Cuts at: 1484 1943 2348 3391 4025 4253 4648 4856 1484
Size: 459 405 1043 634 228 395 208 5330
Fragments arranged by size:
5330 1043 634 459 405 395 228 208
BbvCI CC'TCA_GC
Cuts at: 1939 1939
Size: 8702
BciVI GTATCCnnnnn_n'
Cuts at: 611 2609 4293 6600 7361 8702 611
Size: 1998 1684 2307 761 1341 611
Fragments arranged by size:
2307 1998 1684 1341 761 611
BclI T'GATC_A
Cuts at: 197 6222 197
Size: 6025 2677
BfrBI ATG'CAT
Cuts at: 3532 3532
Size: 8702
BglI GCCn_nnn'nGGC
Cuts at: 2088 2088
Size: 8702
BglII A'GATC_T
Cuts at: 1128 2352 2392 4776 5384 6217 8254 1128
Size: 1224 40 2384 608 833 2037 1576
Fragments arranged by size:
2384 2037 1576 1224 833 608 40
BlpI GC'TnA_GC
Cuts at: 1044 3664 5814 1044
Size: 2620 2150 3932
Fragments arranged by size:
3932 2620 2150
Bme1580I G_kGCm'C
Cuts at: 5420 6327 6420 7476 5420
Size: 907 93 1056 6646
Fragments arranged by size:
6646 1056 907 93
BmrI ACTGGGnnnn_n'
Cuts at: 824 5262 5914 5995 6330 8474 824
Size: 4438 652 81 335 2144 1052
Fragments arranged by size:
4438 2144 1052 652 335 81
BmtI G_CTAG'C
Cuts at: 3989 3989
Size: 8702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7069 7069
Size: 8702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 2156 3258 4312 211
Size: 1945 1102 1054 4601
Fragments arranged by size:
4601 1945 1102 1054
Bpu10I CC'TnA_GC
Cuts at: 1939 3704 4254 1939
Size: 1765 550 6387
Fragments arranged by size:
6387 1765 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 3213 4444 4664 5878 6757 7249 7547 719
Size: 2494 1231 220 1214 879 492 298 1874
Fragments arranged by size:
2494 1874 1231 1214 879 492 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 1620 8406 1620
Size: 6786 1916
BsaAI yAC'GTr
Cuts at: 6055 6055
Size: 8702
BsaBI GATnn'nnATC
Cuts at: 6 6216 8253 6
Size: 6210 2037 455
Fragments arranged by size:
6210 2037 455
BsaHI Gr'CG_yC
Cuts at: 634 6133 6381 7083 8579 634
Size: 5499 248 702 1496 757
Fragments arranged by size:
5499 1496 757 702 248
BsaWI w'CCGG_w
Cuts at: 5891 6412 7090 7364 7511 7922 5891
Size: 521 678 274 147 411 6671
Fragments arranged by size:
6671 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1550 1580 2360 2390 5312 5342 1550
Size: 30 780 30 2922 30 4910
Fragments arranged by size:
4910 2922 780 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1956 3892 4699 4726 5333 1956
Size: 1936 807 27 607 5325
Fragments arranged by size:
5325 1936 807 607 27
BseYI C'CCAG_C
Cuts at: 1108 4975 6127 6885 7462 7958 1108
Size: 3867 1152 758 577 496 1852
Fragments arranged by size:
3867 1852 1152 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 2543 4026 5557 884
Size: 1659 1483 1531 4029
Fragments arranged by size:
4029 1659 1531 1483
BsiEI CG_ry'CG
Cuts at: 104 6244 6290 7498 104
Size: 6140 46 1208 1308
Fragments arranged by size:
6140 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3181 4691 5716 6494 6684 7476 3181
Size: 1510 1025 778 190 792 4407
Fragments arranged by size:
4407 1510 1025 792 778 190
BsmI GAATG_Cn'
Cuts at: 1140 1241 3354 1140
Size: 101 2113 6488
Fragments arranged by size:
6488 2113 101
BsmBI CGTCTCn'nnnn_
Cuts at: 790 4245 790
Size: 3455 5247
Bsp1286I G_dGCh'C
Cuts at: 3181 3274 4691 4860 5420 5716 6327 6420 6494
Size: 93 1417 169 560 296 611 93 74
Cuts at: 6494 6684 6746 7476 3181
Size: 190 62 730 4407
Fragments arranged by size:
4407 1417 730 611 560 296 190 169
93 93 74 62
BspEI T'CCGG_A
Cuts at: 7922 7922
Size: 8702
BspHI T'CATG_A
Cuts at: 1416 1764 7878 1416
Size: 348 6114 2240
Fragments arranged by size:
6114 2240 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2136 4439 5752 5966 6268 6649 2136
Size: 2303 1313 214 302 381 4189
Fragments arranged by size:
4189 2303 1313 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6994 7048 8532 8697 50
Size: 6944 54 1484 165 55
Fragments arranged by size:
6944 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4994 6614 4994
Size: 1620 7082
BsrFI r'CCGG_y
Cuts at: 6700 6881 7090 6700
Size: 181 209 8312
Fragments arranged by size:
8312 209 181
BsrGI T'GTAC_A
Cuts at: 2865 5102 2865
Size: 2237 6465
BssSI C'ACGA_G
Cuts at: 4871 5098 6973 7331 4871
Size: 227 1875 358 6242
Fragments arranged by size:
6242 1875 358 227
BstAPI GCAn_nnn'nTGC
Cuts at: 245 1179 4936 6079 245
Size: 934 3757 1143 2868
Fragments arranged by size:
3757 2868 1143 934
BstBI TT'CG_AA
Cuts at: 7063 7063
Size: 8702
BstEII G'GTnAC_C
Cuts at: 3709 5262 5604 3709
Size: 1553 342 6807
Fragments arranged by size:
6807 1553 342
BstXI CCAn_nnnn'nTGG
Cuts at: 189 4105 5549 5578 189
Size: 3916 1444 29 3313
Fragments arranged by size:
3916 3313 1444 29
BstYI r'GATC_y
Cuts at: 1 655 1128 2352 2392 4047 4776 5384 5678
Size: 654 473 1224 40 1655 729 608 294
Cuts at: 5678 5727 6194 6217 6552 6798 7810 7896 7908
Size: 49 467 23 335 246 1012 86 12
Cuts at: 7908 8254 1
Size: 346 449
Fragments arranged by size:
1655 1224 1012 729 654 608 473 467
449 346 335 294 246 86 49 40
23 12
BstZ17I GTA'TAC
Cuts at: 3046 3046
Size: 8702
Bsu36I CC'TnA_GG
Cuts at: 1649 3380 1649
Size: 1731 6971
BtgI C'CryG_G
Cuts at: 107 5060 107
Size: 4953 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6652 6833 6996 6652
Size: 181 163 8358
Fragments arranged by size:
8358 181 163
BtsI GCAGTG_nn'
Cuts at: 705 2052 2882 4014 5945 705
Size: 1347 830 1132 1931 3462
Fragments arranged by size:
3462 1931 1347 1132 830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 217 252 4727 4762 217
Size: 35 4475 35 4157
Fragments arranged by size:
4475 4157 35 35
DraI TTT'AAA
Cuts at: 3216 5701 3216
Size: 2485 6217
DraIII CAC_nnn'GTG
Cuts at: 221 221
Size: 8702
DrdI GACnn_nn'nnGTC
Cuts at: 6408 7266 6408
Size: 858 7844
EaeI y'GGCC_r
Cuts at: 1403 6287 6461 6852 6879 8224 1403
Size: 4884 174 391 27 1345 1881
Fragments arranged by size:
4884 1881 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6287 6287
Size: 8702
EarI CTCTTCn'nnn_
Cuts at: 128 353 2045 2140 2297 2738 3711 3740 3764
Size: 225 1692 95 157 441 973 29 24
Cuts at: 3764 4172 4419 4721 4822 5147 6725 6935 128
Size: 408 247 302 101 325 1578 210 1895
Fragments arranged by size:
1895 1692 1578 973 441 408 325 302
247 225 210 157 101 95 29 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1116 6910 7220 7366 8512 1116
Size: 5794 310 146 1146 1306
Fragments arranged by size:
5794 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4689 5714 4689
Size: 1025 7677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 689 1229 1367 2156 2227 2360 3258 4265
Size: 478 540 138 789 71 133 898 1007
Cuts at: 4265 4312 4642 4868 5171 5373 6527 6959 7706
Size: 47 330 226 303 202 1154 432 747
Cuts at: 7706 7974 211
Size: 268 939
Fragments arranged by size:
1154 1007 939 898 789 747 540 478
432 330 303 268 226 202 138 133
71 47
EcoNI CCTnn'n_nnAGG
Cuts at: 1634 3964 1634
Size: 2330 6372
EcoO109I rG'GnC_Cy
Cuts at: 277 2777 4428 5417 5841 277
Size: 2500 1651 989 424 3138
Fragments arranged by size:
3138 2500 1651 989 424
EcoRI G'AATT_C
Cuts at: 1015 5706 1015
Size: 4691 4011
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3679 3921 4430 6721 3679
Size: 242 509 2291 5660
Fragments arranged by size:
5660 2291 509 242
FspI TGC'GCA
Cuts at: 6483 6483
Size: 8702
HaeII r_GCGC'y
Cuts at: 2706 6136 6384 7406 8385 2706
Size: 3430 248 1022 979 3023
Fragments arranged by size:
3430 3023 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 609 641 1297 1303 1329 1335 3739
Size: 32 247 32 656 6 26 6 2404
Cuts at: 3739 3771 3847 3879 4819 4851 5312 5344 5479
Size: 32 76 32 940 32 461 32 135
Cuts at: 5479 5511 6544 6574 6576 6606 330
Size: 32 1033 30 2 30 2426
Fragments arranged by size:
2426 2404 1033 940 656 461 247 135
76 32 32 32 32 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 349 2213 2619 5046 5741 349
Size: 1864 406 2427 695 3310
Fragments arranged by size:
3310 2427 1864 695 406
HpaI GTT'AAC
Cuts at: 2213 2619 2213
Size: 406 8296
KasI G'GCGC_C
Cuts at: 6132 6380 6132
Size: 248 8454
KpnI G_GTAC'C
Cuts at: 2425 5722 2425
Size: 3297 5405
MfeI C'AATT_G
Cuts at: 4918 5525 5576 4918
Size: 607 51 8044
Fragments arranged by size:
8044 607 51
MluI A'CGCG_T
Cuts at: 8135 8135
Size: 8702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 594 3810 5603 7373 7557 594
Size: 3216 1793 1770 184 1739
Fragments arranged by size:
3216 1793 1770 1739 184
MscI TGG'CCA
Cuts at: 1405 6463 1405
Size: 5058 3644
MslI CAynn'nnrTG
Cuts at: 1967 2644 3962 4218 5065 5428 6691 6818 1967
Size: 677 1318 256 847 363 1263 127 3851
Fragments arranged by size:
3851 1318 1263 847 677 363 256 127
MspA1I CmG'CkG
Cuts at: 615 868 4722 4951 5463 5813 6127 6487 7500
Size: 253 3854 229 512 350 314 360 1013
Cuts at: 7500 7745 615
Size: 245 1572
Fragments arranged by size:
3854 1572 1013 512 360 350 314 253
245 229
NaeI GCC'GGC
Cuts at: 6883 6883
Size: 8702
NarI GG'CG_CC
Cuts at: 6133 6381 6133
Size: 248 8454
NcoI C'CATG_G
Cuts at: 107 5060 107
Size: 4953 3749
NgoMIV G'CCGG_C
Cuts at: 6881 6881
Size: 8702
NheI G'CTAG_C
Cuts at: 3985 3985
Size: 8702
NsiI A_TGCA'T
Cuts at: 3534 3534
Size: 8702
NspI r_CATG'y
Cuts at: 252 919 1091 4638 5433 5755 6255 7162 252
Size: 667 172 3547 795 322 500 907 1792
Fragments arranged by size:
3547 1792 907 795 667 500 322 172
PciI A'CATG_T
Cuts at: 1087 7158 1087
Size: 6071 2631
PflMI CCAn_nnn'nTGG
Cuts at: 489 5655 489
Size: 5166 3536
PfoI T'CCnGG_A
Cuts at: 2730 3402 2730
Size: 672 8030
PmeI GTTT'AAAC
Cuts at: 5701 5701
Size: 8702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7841 7873 7841
Size: 32 8670
PpuMI rG'GwC_Cy
Cuts at: 2777 4428 2777
Size: 1651 7051
PshAI GACnn'nnGTC
Cuts at: 637 637
Size: 8702
PspOMI G'GGCC_C
Cuts at: 5416 5416
Size: 8702
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1334 1366 1334
Size: 32 8670
PstI C_TGCA'G
Cuts at: 2889 3919 4551 5749 6434 2889
Size: 1030 632 1198 685 5157
Fragments arranged by size:
5157 1198 1030 685 632
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8702
PvuII CAG'CTG
Cuts at: 868 4722 4951 5463 6127 6487 868
Size: 3854 229 512 664 360 3083
Fragments arranged by size:
3854 3083 664 512 360 229
RsrII CG'GwC_CG
Cuts at: 6897 6897
Size: 8702
SacI G_AGCT'C
Cuts at: 4691 5716 4691
Size: 1025 7677
SalI G'TCGA_C
Cuts at: 5739 5739
Size: 8702
SapI GCTCTTCn'nnn_
Cuts at: 128 353 3764 4172 6725 6935 128
Size: 225 3411 408 2553 210 1895
Fragments arranged by size:
3411 2553 1895 408 225 210
SbfI CC_TGCA'GG
Cuts at: 5749 5749
Size: 8702
ScaI AGT'ACT
Cuts at: 3258 8219 3258
Size: 4961 3741
SexAI A'CCwGG_T
Cuts at: 2223 2424 3923 2223
Size: 201 1499 7002
Fragments arranged by size:
7002 1499 201
SfcI C'TryA_G
Cuts at: 33 495 520 2396 2885 3915 4547 5745 6430
Size: 462 25 1876 489 1030 632 1198 685
Cuts at: 6430 7423 7614 33
Size: 993 191 1121
Fragments arranged by size:
1876 1198 1121 1030 993 685 632 489
462 191 25
SfoI GGC'GCC
Cuts at: 6134 6382 6134
Size: 248 8454
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8702
SmaI CCC'GGG
Cuts at: 5724 5724
Size: 8702
SmlI C'TyrA_G
Cuts at: 734 1527 3228 4423 4679 5857 6772 7264 7526
Size: 793 1701 1195 256 1178 915 492 262
Cuts at: 7526 734
Size: 1910
Fragments arranged by size:
1910 1701 1195 1178 915 793 492 262
256
SpeI A'CTAG_T
Cuts at: 67 1980 3595 67
Size: 1913 1615 5174
Fragments arranged by size:
5174 1913 1615
SphI G_CATG'C
Cuts at: 252 5755 6255 252
Size: 5503 500 2699
Fragments arranged by size:
5503 2699 500
SspI AAT'ATT
Cuts at: 1372 1473 1487 1749 3237 3932 5215 1372
Size: 101 14 262 1488 695 1283 4859
Fragments arranged by size:
4859 1488 1283 695 262 101 14
StuI AGG'CCT
Cuts at: 1640 4520 1640
Size: 2880 5822
StyI C'CwwG_G
Cuts at: 107 582 1219 2540 4768 5060 5346 5505 5836
Size: 475 637 1321 2228 292 286 159 331
Cuts at: 5836 6190 107
Size: 354 2619
Fragments arranged by size:
2619 2228 1321 637 475 354 331 292
286 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3703 4057 4208 5250 5537 5607 6285 6418 6877
Size: 354 151 1042 287 70 678 133 459
Cuts at: 6877 7086 3703
Size: 209 5319
Fragments arranged by size:
5319 1042 678 459 354 287 209 151
133 70
TatI w'GTAC_w
Cuts at: 138 2014 2069 2865 3169 3256 5102 8217 138
Size: 1876 55 796 304 87 1846 3115 623
Fragments arranged by size:
3115 1876 1846 796 623 304 87 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2761 2793 4032 4064 8219 8251 2761
Size: 32 1239 32 4155 32 3212
Fragments arranged by size:
4155 3212 1239 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5268 6499 5268
Size: 1231 7471
XbaI T'CTAG_A
Cuts at: 4779 5733 4779
Size: 954 7748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5442 6197 5442
Size: 755 7947
XmaI C'CCGG_G
Cuts at: 5722 5722
Size: 8702
XmnI GAAnn'nnTTC
Cuts at: 1214 1445 2686 3308 3455 3773 1214
Size: 231 1241 622 147 318 6143
Fragments arranged by size:
6143 1241 622 318 231 147
ZraI GAC'GTC
Cuts at: 635 635
Size: 8702
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI AscI AvrII BcgI BmgBI
BsiWI BssHII ClaI EcoRV FseI FspAI HindIII NdeI
NotI NruI PacI PasI PmlI PsiI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI