Restriction map for
pF1KB5597
(Circular) (Six-base) MAPSORT of: pF1KB5597.seq Check: 6644 from: 1 to: 4499
pF1KB5597 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1077 1077
Size: 4499
AccI GT'mk_AC
Cuts at: 1332 1537 1332
Size: 205 4294
Acc65I G'GTAC_C
Cuts at: 1172 1515 1172
Size: 343 4156
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1315 2324 2756 3109 3771 469
Size: 846 1009 432 353 662 1197
Fragments arranged by size:
1197 1009 846 662 432 353
AflIII A'CryG_T
Cuts at: 1851 3651 3932 1851
Size: 1800 281 2418
Fragments arranged by size:
2418 1800 281
AleI CACnn'nnGTG
Cuts at: 418 418
Size: 4499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 129 160 445 756 807 921 1074 1143 1804
Size: 31 285 311 51 114 153 69 661
Cuts at: 1804 3242 129
Size: 1438 1386
Fragments arranged by size:
1438 1386 661 311 285 153 114 69
51 31
ApaLI G'TGCA_C
Cuts at: 3337 3337
Size: 4499
ApoI r'AATT_y
Cuts at: 589 1503 3852 4108 589
Size: 914 2349 256 980
Fragments arranged by size:
2349 980 914 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 320 1519 320
Size: 1199 3300
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 226 1037 1172 1382 1515 1929 2177 2212 226
Size: 811 135 210 133 414 248 35 2513
Fragments arranged by size:
2513 811 414 248 210 135 133 35
BanII G_rGCy'C
Cuts at: 1120 1513 2543 1120
Size: 393 1030 3076
Fragments arranged by size:
3076 1030 393
BbeI G_GCGC'C
Cuts at: 1041 1386 1933 2181 1041
Size: 345 547 248 3359
Fragments arranged by size:
3359 547 345 248
BbsI GAAGACnn'nnnn_
Cuts at: 1422 1422
Size: 4499
BbvCI CC'TCA_GC
Cuts at: 743 1387 1469 743
Size: 644 82 3773
Fragments arranged by size:
3773 644 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 840 874 840
Size: 34 4465
BciVI GTATCCnnnnn_n'
Cuts at: 408 2397 3453 4499 408
Size: 1989 1056 1046 408
Fragments arranged by size:
1989 1056 1046 408
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 676 862 676
Size: 186 4313
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 757 1611 757
Size: 854 3645
Bme1580I G_kGCm'C
Cuts at: 229 2124 2217 3341 229
Size: 1895 93 1124 1387
Fragments arranged by size:
1895 1387 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 363 919 1267 237
Size: 126 556 348 3469
Fragments arranged by size:
3469 556 348 126
Bpu10I CC'TnA_GC
Cuts at: 743 1387 1469 743
Size: 644 82 3773
Fragments arranged by size:
3773 644 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1675 2554 3268 3566 970
Size: 705 879 714 298 1903
Fragments arranged by size:
1903 879 714 705 298
BsaI GGTCTCn'nnnn_
Cuts at: 787 4203 787
Size: 3416 1083
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1038 1383 1930 2178 2880 4376 1038
Size: 345 547 248 702 1496 1161
Fragments arranged by size:
1496 1161 702 547 345 248
BsaWI w'CCGG_w
Cuts at: 1122 1688 2209 2887 3298 3445 3719 1122
Size: 566 521 678 411 147 274 1902
Fragments arranged by size:
1902 678 566 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 616 646 553
Size: 30 33 30 4406
Fragments arranged by size:
4406 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 201 240 366 760 196
Size: 5 39 126 394 3935
Fragments arranged by size:
3935 394 126 39 5
BseYI C'CCAG_C
Cuts at: 409 427 432 438 1924 2682 3347 3755 409
Size: 18 5 6 1486 758 665 408 1153
Fragments arranged by size:
1486 1153 758 665 408 18 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 796 934 676
Size: 120 138 4241
Fragments arranged by size:
4241 138 120
BsiEI CG_ry'CG
Cuts at: 104 1360 2041 2087 3317 104
Size: 1256 681 46 1230 1286
Fragments arranged by size:
1286 1256 1230 681 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1367 1513 2291 2481 3341 1120
Size: 247 146 778 190 860 2278
Fragments arranged by size:
2278 860 778 247 190 146
Bsp1286I G_dGCh'C
Cuts at: 229 1120 1367 1513 2124 2217 2291 2481 2543
Size: 891 247 146 611 93 74 190 62
Cuts at: 2543 3341 229
Size: 798 1387
Fragments arranged by size:
1387 891 798 611 247 190 146 93
74 62
BspEI T'CCGG_A
Cuts at: 1122 1122
Size: 4499
BspHI T'CATG_A
Cuts at: 878 2931 878
Size: 2053 2446
BspMI ACCTGCnnnn'nnnn_
Cuts at: 815 902 1077 1549 1763 2065 2446 815
Size: 87 175 472 214 302 381 2868
Fragments arranged by size:
2868 472 381 302 214 175 87
BsrBI CCG'CTC
Cuts at: 50 170 2791 2845 4329 4494 50
Size: 120 2621 54 1484 165 55
Fragments arranged by size:
2621 1484 165 120 55 54
BsrDI GCAATG_nn'
Cuts at: 1316 1451 2411 1316
Size: 135 960 3404
Fragments arranged by size:
3404 960 135
BsrFI r'CCGG_y
Cuts at: 1034 1359 2497 2678 1034
Size: 325 1138 181 2855
Fragments arranged by size:
2855 1138 325 181
BssSI C'ACGA_G
Cuts at: 2770 3478 2770
Size: 708 3791
BstAPI GCAn_nnn'nTGC
Cuts at: 921 1876 921
Size: 955 3544
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstEII G'GTnAC_C
Cuts at: 489 489
Size: 4499
BstXI CCAn_nnnn'nTGG
Cuts at: 342 501 342
Size: 159 4340
BstYI r'GATC_y
Cuts at: 1 498 1524 1991 2014 2349 2595 2901 2913
Size: 497 1026 467 23 335 246 306 12
Cuts at: 2913 2999 4051 1
Size: 86 1052 449
Fragments arranged by size:
1052 1026 497 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 158 1154 1742 158
Size: 996 588 2915
Fragments arranged by size:
2915 996 588
ClaI AT'CG_AT
Cuts at: 1349 1349
Size: 4499
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3549 2205
Size: 1344 3155
EaeI y'GGCC_r
Cuts at: 1357 2084 2258 2649 2676 4021 1357
Size: 727 174 391 27 1345 1835
Fragments arranged by size:
1835 1345 727 391 174 27
EagI C'GGCC_G
Cuts at: 1357 2084 1357
Size: 727 3772
EarI CTCTTCn'nnn_
Cuts at: 463 2522 2732 463
Size: 2059 210 2230
Fragments arranged by size:
2230 2059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3449 3595 4309 2707
Size: 742 146 714 2897
Fragments arranged by size:
2897 742 714 146
EcoICRI GAG'CTC
Cuts at: 1118 1511 1118
Size: 393 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 363 469 919 1267 1315 2324 2756 3109
Size: 126 106 450 348 48 1009 432 353
Cuts at: 3109 3771 237
Size: 662 965
Fragments arranged by size:
1009 965 662 450 432 353 348 126
106 48
EcoNI CCTnn'n_nnAGG
Cuts at: 613 898 613
Size: 285 4214
EcoO109I rG'GnC_Cy
Cuts at: 726 804 855 963 1005 1446 1638 726
Size: 78 51 108 42 441 192 3587
Fragments arranged by size:
3587 441 192 108 78 51 42
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
EcoRV GAT'ATC
Cuts at: 1268 1268
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 357 1041 1386 1933 2181 3411 4182 357
Size: 684 345 547 248 1230 771 674
Fragments arranged by size:
1230 771 684 674 547 345 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 483 515 553 585 989 1021 1325
Size: 32 282 32 38 32 404 32 304
Cuts at: 1325 1357 2341 2371 2373 2403 169
Size: 32 984 30 2 30 2265
Fragments arranged by size:
2265 984 404 304 282 38 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1333 1538 1333
Size: 205 4294
KasI G'GCGC_C
Cuts at: 1037 1382 1929 2177 1037
Size: 345 547 248 3359
Fragments arranged by size:
3359 547 345 248
KpnI G_GTAC'C
Cuts at: 1176 1519 1176
Size: 343 4156
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3258 3442 3258
Size: 184 4315
MscI TGG'CCA
Cuts at: 2260 2260
Size: 4499
MslI CAynn'nnrTG
Cuts at: 418 824 1111 2488 2615 418
Size: 406 287 1377 127 2302
Fragments arranged by size:
2302 1377 406 287 127
MspA1I CmG'CkG
Cuts at: 509 596 690 753 921 1062 1610 1924 2284
Size: 87 94 63 168 141 548 314 360
Cuts at: 2284 3068 3313 509
Size: 784 245 1695
Fragments arranged by size:
1695 784 548 360 314 245 168 141
94 87 63
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1038 1383 1930 2178 1038
Size: 345 547 248 3359
Fragments arranged by size:
3359 547 345 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 996 1552 2052 3655 996
Size: 556 500 1603 1840
Fragments arranged by size:
1840 1603 556 500
PasI CC'CwG_GG
Cuts at: 1200 1200
Size: 4499
PciI A'CATG_T
Cuts at: 3651 3651
Size: 4499
PflMI CCAn_nnn'nTGG
Cuts at: 222 445 1143 222
Size: 223 698 3578
Fragments arranged by size:
3578 698 223
PfoI T'CCnGG_A
Cuts at: 620 800 1007 1270 620
Size: 180 207 263 3849
Fragments arranged by size:
3849 263 207 180
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 804 963 1005 1446 804
Size: 159 42 441 3857
Fragments arranged by size:
3857 441 159 42
PshAI GACnn'nnGTC
Cuts at: 571 571
Size: 4499
PstI C_TGCA'G
Cuts at: 522 863 1546 2231 522
Size: 341 683 685 2790
Fragments arranged by size:
2790 685 683 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 596 690 753 1062 1924 2284 596
Size: 94 63 309 862 360 2811
Fragments arranged by size:
2811 862 360 309 94 63
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1120 1513 1120
Size: 393 4106
SalI G'TCGA_C
Cuts at: 1331 1536 1331
Size: 205 4294
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 863 1546 863
Size: 683 3816
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 518 859 1542 2227 3195 3386 33
Size: 485 341 683 685 968 191 1146
Fragments arranged by size:
1146 968 685 683 485 341 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 676 862 676
Size: 186 4313
SfoI GGC'GCC
Cuts at: 1039 1384 1931 2179 1039
Size: 345 547 248 3359
Fragments arranged by size:
3359 547 345 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 322 1521 322
Size: 1199 3300
SmlI C'TyrA_G
Cuts at: 985 1654 2569 3283 3545 985
Size: 669 915 714 262 1939
Fragments arranged by size:
1939 915 714 669 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StuI AGG'CCT
Cuts at: 220 220
Size: 4499
StyI C'CwwG_G
Cuts at: 107 221 494 788 1214 1633 1987 107
Size: 114 273 294 426 419 354 2619
Fragments arranged by size:
2619 426 419 354 294 273 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 710 2082 2215 2674 2883 441
Size: 269 1372 133 459 209 2057
Fragments arranged by size:
2057 1372 459 269 209 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1266 1298 1346 1378 4016 4048 1266
Size: 32 48 32 2638 32 1717
Fragments arranged by size:
2638 1717 48 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 320 1519 320
Size: 1199 3300
XmnI GAAnn'nnTTC
Cuts at: 274 274
Size: 4499
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI