Restriction map for
pF1KB5599
(Circular) (Six-base) MAPSORT of: pF1KB5599.seq Check: 5551 from: 1 to: 5909
pF1KB5599 5909 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1173 2350 1173
Size: 1177 4732
AccI GT'mk_AC
Cuts at: 2947 2947
Size: 5909
Acc65I G'GTAC_C
Cuts at: 796 2925 796
Size: 2129 3780
AclI AA'CG_TT
Cuts at: 5749 5749
Size: 5909
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 1994 3734 4166 4519 5181 1903
Size: 91 1740 432 353 662 2631
Fragments arranged by size:
2631 1740 662 432 353 91
AflIII A'CryG_T
Cuts at: 3261 5061 5342 3261
Size: 1800 281 3828
Fragments arranged by size:
3828 1800 281
AleI CACnn'nnGTG
Cuts at: 211 448 211
Size: 237 5672
AlwNI CAG_nnn'CTG
Cuts at: 766 2040 2785 3214 4652 766
Size: 1274 745 429 1438 2023
Fragments arranged by size:
2023 1438 1274 745 429
ApaLI G'TGCA_C
Cuts at: 4747 4747
Size: 5909
ApoI r'AATT_y
Cuts at: 412 472 811 1321 1401 2619 2628 2913 5262
Size: 60 339 510 80 1218 9 285 2349
Cuts at: 5262 5518 412
Size: 256 803
Fragments arranged by size:
2349 1218 803 510 339 285 256 80
60 9
AseI AT'TA_AT
Cuts at: 20 722 20
Size: 702 5207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
AvaI C'yCGr_G
Cuts at: 2929 2929
Size: 5909
BamHI G'GATC_C
Cuts at: 2934 2934
Size: 5909
BanI G'GyrC_C
Cuts at: 796 2925 3339 3587 3622 796
Size: 2129 414 248 35 3083
Fragments arranged by size:
3083 2129 414 248 35
BanII G_rGCy'C
Cuts at: 236 1302 1751 2923 3953 236
Size: 1066 449 1172 1030 2192
Fragments arranged by size:
2192 1172 1066 1030 449
BbeI G_GCGC'C
Cuts at: 3343 3591 3343
Size: 248 5661
BbsI GAAGACnn'nnnn_
Cuts at: 1393 1547 2600 1393
Size: 154 1053 4702
Fragments arranged by size:
4702 1053 154
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3807 4863 5909 2367
Size: 1440 1056 1046 2367
Fragments arranged by size:
2367 1440 1056 1046
BclI T'GATC_A
Cuts at: 288 2598 3429 288
Size: 2310 831 2768
Fragments arranged by size:
2768 2310 831
BglII A'GATC_T
Cuts at: 3424 5461 3424
Size: 2037 3872
BlpI GC'TnA_GC
Cuts at: 3021 3021
Size: 5909
Bme1580I G_kGCm'C
Cuts at: 584 3534 3627 4751 584
Size: 2950 93 1124 1742
Fragments arranged by size:
2950 1742 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1305 3121 3202 3537 5681 1305
Size: 1816 81 335 2144 1533
Fragments arranged by size:
2144 1816 1533 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4276 4276
Size: 5909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 2306 962
Size: 1344 4565
Bpu10I CC'TnA_GC
Cuts at: 1865 1865
Size: 5909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3085 3964 4678 4976 3085
Size: 879 714 298 4018
Fragments arranged by size:
4018 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5613 5613
Size: 5909
BsaAI yAC'GTr
Cuts at: 3262 3262
Size: 5909
BsaBI GATnn'nnATC
Cuts at: 6 2495 2798 3423 5460 6
Size: 2489 303 625 2037 455
Fragments arranged by size:
2489 2037 625 455 303
BsaHI Gr'CG_yC
Cuts at: 3340 3588 4290 5786 3340
Size: 248 702 1496 3463
Fragments arranged by size:
3463 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3098 3619 4297 4708 4855 5129 3098
Size: 521 678 411 147 274 3878
Fragments arranged by size:
3878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 935 965 2316 2346 935
Size: 30 1351 30 4498
Fragments arranged by size:
4498 1351 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 1816 2461 2470 262
Size: 1554 645 9 3701
Fragments arranged by size:
3701 1554 645 9
BseYI C'CCAG_C
Cuts at: 149 2813 3334 4092 4757 5165 149
Size: 2664 521 758 665 408 893
Fragments arranged by size:
2664 893 758 665 521 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 812
Size: 5909
BsiEI CG_ry'CG
Cuts at: 104 126 185 3451 3497 4727 104
Size: 22 59 3266 46 1230 1286
Fragments arranged by size:
3266 1286 1230 59 46 22
BsiHKAI G_wGCw'C
Cuts at: 2471 2923 3701 3891 4751 2471
Size: 452 778 190 860 3629
Fragments arranged by size:
3629 860 778 452 190
BsmI GAATG_Cn'
Cuts at: 1140 2286 1140
Size: 1146 4763
Bsp1286I G_dGCh'C
Cuts at: 236 584 1302 1751 2471 2923 3534 3627 3701
Size: 348 718 449 720 452 611 93 74
Cuts at: 3701 3891 3953 4751 236
Size: 190 62 798 1394
Fragments arranged by size:
1394 798 720 718 611 452 449 348
190 93 74 62
BspHI T'CATG_A
Cuts at: 1221 1467 4341 1221
Size: 246 2874 2789
Fragments arranged by size:
2874 2789 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 784 1173 1215 2350 2959 3173 3475 3856 784
Size: 389 42 1135 609 214 302 381 2837
Fragments arranged by size:
2837 1135 609 389 381 302 214 42
BsrBI CCG'CTC
Cuts at: 50 4201 4255 5739 5904 50
Size: 4151 54 1484 165 55
Fragments arranged by size:
4151 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3821 3821
Size: 5909
BsrFI r'CCGG_y
Cuts at: 118 681 3907 4088 118
Size: 563 3226 181 1939
Fragments arranged by size:
3226 1939 563 181
BsrGI T'GTAC_A
Cuts at: 781 2055 2746 781
Size: 1274 691 3944
Fragments arranged by size:
3944 1274 691
BssSI C'ACGA_G
Cuts at: 2048 4180 4888 2048
Size: 2132 708 3069
Fragments arranged by size:
3069 2132 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3286 3286
Size: 5909
BstBI TT'CG_AA
Cuts at: 4270 4270
Size: 5909
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2284
Size: 5909
BstYI r'GATC_y
Cuts at: 1 1362 1703 2124 2390 2496 2934 3401 3424
Size: 1361 341 421 266 106 438 467 23
Cuts at: 3424 3759 4005 4311 4323 4409 5461 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1361 1052 467 449 438 421 341 335
306 266 246 106 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 174 3859 4040 4203 174
Size: 3685 181 163 1880
Fragments arranged by size:
3685 1880 181 163
BtsI GCAGTG_nn'
Cuts at: 491 631 2216 2337 3152 491
Size: 140 1585 121 815 3248
Fragments arranged by size:
3248 1585 815 140 121
DraI TTT'AAA
Cuts at: 458 728 1406 1509 2908 458
Size: 270 678 103 1399 3459
Fragments arranged by size:
3459 1399 678 270 103
DrdI GACnn_nn'nnGTC
Cuts at: 3615 4959 3615
Size: 1344 4565
EaeI y'GGCC_r
Cuts at: 123 182 203 1853 3494 3668 4059 4086 5431
Size: 59 21 1650 1641 174 391 27 1345
Cuts at: 5431 123
Size: 601
Fragments arranged by size:
1650 1641 1345 601 391 174 59 27
21
EagI C'GGCC_G
Cuts at: 123 182 3494 123
Size: 59 3312 2538
Fragments arranged by size:
3312 2538 59
EarI CTCTTCn'nnn_
Cuts at: 1103 3932 4142 1103
Size: 2829 210 2870
Fragments arranged by size:
2870 2829 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4117 4859 5005 5719 4117
Size: 742 146 714 4307
Fragments arranged by size:
4307 742 714 146
EcoICRI GAG'CTC
Cuts at: 2921 2921
Size: 5909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 1903 1994 2306 3734 4166 4519 5181 962
Size: 941 91 312 1428 432 353 662 1690
Fragments arranged by size:
1690 1428 941 662 432 353 312 91
EcoO109I rG'GnC_Cy
Cuts at: 137 3048 137
Size: 2911 2998
EcoRI G'AATT_C
Cuts at: 2913 2913
Size: 5909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3928 3928
Size: 5909
FspI TGC'GCA
Cuts at: 3690 3690
Size: 5909
HaeII r_GCGC'y
Cuts at: 3343 3591 4821 5592 3343
Size: 248 1230 771 3660
Fragments arranged by size:
3660 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1775 1807 2590 2622 2688 2715 2720 2747 3751
Size: 32 783 32 66 27 5 27 1004
Cuts at: 3751 3781 3783 3813 1775
Size: 30 2 30 3871
Fragments arranged by size:
3871 1004 783 66 32 32 30 30
27 27 5 2
HincII GTy'rAC
Cuts at: 2948 2948
Size: 5909
HindIII A'AGCT_T
Cuts at: 259 259
Size: 5909
KasI G'GCGC_C
Cuts at: 3339 3587 3339
Size: 248 5661
KpnI G_GTAC'C
Cuts at: 800 2929 800
Size: 2129 3780
MluI A'CGCG_T
Cuts at: 5342 5342
Size: 5909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1962 4668 4852 951
Size: 1011 2706 184 2008
Fragments arranged by size:
2706 2008 1011 184
MscI TGG'CCA
Cuts at: 1855 3670 1855
Size: 1815 4094
MslI CAynn'nnrTG
Cuts at: 211 296 448 1064 3898 4025 211
Size: 85 152 616 2834 127 2095
Fragments arranged by size:
2834 2095 616 152 127 85
MspA1I CmG'CkG
Cuts at: 2040 3020 3334 3694 4478 4723 2040
Size: 980 314 360 784 245 3226
Fragments arranged by size:
3226 980 784 360 314 245
NaeI GCC'GGC
Cuts at: 120 4090 120
Size: 3970 1939
NarI GG'CG_CC
Cuts at: 3340 3588 3340
Size: 248 5661
NgoMIV G'CCGG_C
Cuts at: 118 4088 118
Size: 3970 1939
NspI r_CATG'y
Cuts at: 319 1945 2253 2962 3462 5065 319
Size: 1626 308 709 500 1603 1163
Fragments arranged by size:
1626 1603 1163 709 500 308
PciI A'CATG_T
Cuts at: 5061 5061
Size: 5909
PflMI CCAn_nnn'nTGG
Cuts at: 2080 2179 2080
Size: 99 5810
PfoI T'CCnGG_A
Cuts at: 2444 2444
Size: 5909
PmeI GTTT'AAAC
Cuts at: 2908 2908
Size: 5909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1630 1662 4355 4387 1630
Size: 32 2693 32 3152
Fragments arranged by size:
3152 2693 32 32
PpuMI rG'GwC_Cy
Cuts at: 137 137
Size: 5909
PstI C_TGCA'G
Cuts at: 2956 3641 2956
Size: 685 5224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5909
PvuII CAG'CTG
Cuts at: 2040 3334 3694 2040
Size: 1294 360 4255
Fragments arranged by size:
4255 1294 360
RsrII CG'GwC_CG
Cuts at: 4104 4104
Size: 5909
SacI G_AGCT'C
Cuts at: 2923 2923
Size: 5909
SalI G'TCGA_C
Cuts at: 2946 2946
Size: 5909
SanDI GG'GwC_CC
Cuts at: 137 137
Size: 5909
SapI GCTCTTCn'nnn_
Cuts at: 3932 4142 3932
Size: 210 5699
SbfI CC_TGCA'GG
Cuts at: 2956 2956
Size: 5909
ScaI AGT'ACT
Cuts at: 275 5426 275
Size: 5151 758
SfcI C'TryA_G
Cuts at: 33 351 1355 2834 2952 3637 4605 4796 33
Size: 318 1004 1479 118 685 968 191 1146
Fragments arranged by size:
1479 1146 1004 968 685 318 191 118
SfoI GGC'GCC
Cuts at: 3341 3589 3341
Size: 248 5661
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
SmaI CCC'GGG
Cuts at: 2931 2931
Size: 5909
SmlI C'TyrA_G
Cuts at: 3064 3979 4693 4955 3064
Size: 915 714 262 4018
Fragments arranged by size:
4018 915 714 262
SpeI A'CTAG_T
Cuts at: 67 168 67
Size: 101 5808
SphI G_CATG'C
Cuts at: 2962 3462 2962
Size: 500 5409
SspI AAT'ATT
Cuts at: 848 933 848
Size: 85 5824
StyI C'CwwG_G
Cuts at: 404 3043 3397 404
Size: 2639 354 2916
Fragments arranged by size:
2916 2639 354
SwaI ATTT'AAAT
Cuts at: 1406 1406
Size: 5909
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 2087 2651 3492 3625 4084 4293 565
Size: 1522 564 841 133 459 209 2181
Fragments arranged by size:
2181 1522 841 564 459 209 133
TatI w'GTAC_w
Cuts at: 273 781 1290 2055 2746 5424 273
Size: 508 509 765 691 2678 758
Fragments arranged by size:
2678 765 758 691 509 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2316 2348 5426 5458 2258
Size: 32 26 32 3078 32 2709
Fragments arranged by size:
3078 2709 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 3706 3706
Size: 5909
XbaI T'CTAG_A
Cuts at: 2940 2940
Size: 5909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1848 2077 3404 1848
Size: 229 1327 4353
Fragments arranged by size:
4353 1327 229
XmaI C'CCGG_G
Cuts at: 2929 2929
Size: 5909
XmnI GAAnn'nnTTC
Cuts at: 414 1482 414
Size: 1068 4841
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI XhoI
ZraI