Restriction map for
pF1KB5605
(Circular) (Six-base) MAPSORT of: pF1KB5605.seq Check: 9748 from: 1 to: 4838
pF1KB5605 4838 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 458 637 1730 458
Size: 179 1093 3566
Fragments arranged by size:
3566 1093 179
AccI GT'mk_AC
Cuts at: 1876 1876
Size: 4838
Acc65I G'GTAC_C
Cuts at: 1486 1854 1486
Size: 368 4470
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1200 1412 2663 3095 3842 4110 1200
Size: 212 1251 432 747 268 1928
Fragments arranged by size:
1928 1251 747 432 268 212
AfeI AGC'GCT
Cuts at: 1123 1123
Size: 4838
AflIII A'CryG_T
Cuts at: 869 2190 3294 4271 869
Size: 1321 1104 977 1436
Fragments arranged by size:
1436 1321 1104 977
AgeI A'CCGG_T
Cuts at: 1070 3226 1070
Size: 2156 2682
AhdI GACnn_n'nnGTC
Cuts at: 1343 1343
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 726 1248 2143 3710 726
Size: 522 895 1567 1854
Fragments arranged by size:
1854 1567 895 522
ApaI G_GGCC'C
Cuts at: 232 419 1511 232
Size: 187 1092 3559
Fragments arranged by size:
3559 1092 187
ApaLI G'TGCA_C
Cuts at: 777 3608 777
Size: 2831 2007
ApoI r'AATT_y
Cuts at: 1842 4191 4447 1842
Size: 2349 256 2233
Fragments arranged by size:
2349 2233 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 225 412 1858 225
Size: 187 1446 3205
Fragments arranged by size:
3205 1446 187
BamHI G'GATC_C
Cuts at: 1863 1863
Size: 4838
BanI G'GyrC_C
Cuts at: 359 655 688 822 916 1316 1486 1537 1738
Size: 296 33 134 94 400 170 51 201
Cuts at: 1738 1854 2268 2516 2551 359
Size: 116 414 248 35 2646
Fragments arranged by size:
2646 414 400 296 248 201 170 134
116 94 51 35 33
BanII G_rGCy'C
Cuts at: 232 419 498 653 894 1021 1106 1202 1511
Size: 187 79 155 241 127 85 96 309
Cuts at: 1511 1592 1643 1852 2882 232
Size: 81 51 209 1030 2188
Fragments arranged by size:
2188 1030 309 241 209 187 155 127
96 85 81 79 51
BbeI G_GCGC'C
Cuts at: 363 659 826 920 1742 2272 2520 363
Size: 296 167 94 822 530 248 2681
Fragments arranged by size:
2681 822 530 296 248 167 94
BbvCI CC'TCA_GC
Cuts at: 536 536
Size: 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 678 712 1413 1447 1656 1690 90
Size: 34 554 34 701 34 209 34 3238
Fragments arranged by size:
3238 701 554 209 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3497 4838 2736
Size: 761 1341 2736
Fragments arranged by size:
2736 1341 761
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 751 820 914 928 1394 1535 1747 751
Size: 69 94 14 466 141 212 3842
Fragments arranged by size:
3842 466 212 141 94 69 14
BglII A'GATC_T
Cuts at: 257 2353 4390 257
Size: 2096 2037 705
Fragments arranged by size:
2096 2037 705
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 232 419 477 551 781 809 910 1390 1511
Size: 187 58 74 230 28 101 480 121
Cuts at: 1511 2463 2556 3612 232
Size: 952 93 1056 1458
Fragments arranged by size:
1458 1056 952 480 230 187 121 101
93 74 58 28
BmgBI CAC'GTC
Cuts at: 1083 1083
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 819 1100 2050 2131 2466 4610 819
Size: 281 950 81 335 2144 1047
Fragments arranged by size:
2144 1047 950 335 281 81
BmtI G_CTAG'C
Cuts at: 1379 1379
Size: 4838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 4838
Bpu10I CC'TnA_GC
Cuts at: 536 536
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2014 2893 3385 3683 2014
Size: 879 492 298 3169
Fragments arranged by size:
3169 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1033 4542 1033
Size: 3509 1329
BsaAI yAC'GTr
Cuts at: 1803 2191 1803
Size: 388 4450
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 360 656 823 917 1606 1739 1819 2269 2517
Size: 296 167 94 689 133 80 450 248
Cuts at: 2517 3219 4715 360
Size: 702 1496 483
Fragments arranged by size:
1496 702 689 483 450 296 248 167
133 94 80
BsaWI w'CCGG_w
Cuts at: 1070 2027 2548 3226 3500 3647 4058 1070
Size: 957 521 678 274 147 411 1850
Fragments arranged by size:
1850 957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 925 955 1534 1564 1608 1638 1750
Size: 30 333 30 579 30 44 30 112
Cuts at: 1750 1780 562
Size: 30 3620
Fragments arranged by size:
3620 579 333 112 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 288 583 1616 1702 288
Size: 295 1033 86 3424
Fragments arranged by size:
3424 1033 295 86
BseYI C'CCAG_C
Cuts at: 550 621 909 1597 2263 3021 3598 4094 550
Size: 71 288 688 666 758 577 496 1294
Fragments arranged by size:
1294 758 688 666 577 496 288 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 1407 930
Size: 477 4361
BsiEI CG_ry'CG
Cuts at: 104 1061 1634 2380 2426 3634 104
Size: 957 573 746 46 1208 1308
Fragments arranged by size:
1308 1208 957 746 573 46
BsiHKAI G_wGCw'C
Cuts at: 206 781 869 989 1852 2630 2820 3612 206
Size: 575 88 120 863 778 190 792 1432
Fragments arranged by size:
1432 863 792 778 575 190 120 88
BsmI GAATG_Cn'
Cuts at: 1647 1647
Size: 4838
BsmBI CGTCTCn'nnnn_
Cuts at: 678 678
Size: 4838
Bsp1286I G_dGCh'C
Cuts at: 206 232 419 477 498 551 653 781 809
Size: 26 187 58 21 53 102 128 28
Cuts at: 809 869 894 910 989 1021 1106 1202 1390
Size: 60 25 16 79 32 85 96 188
Cuts at: 1390 1511 1592 1643 1852 2463 2556 2630 2820
Size: 121 81 51 209 611 93 74 190
Cuts at: 2820 2882 3612 206
Size: 62 730 1432
Fragments arranged by size:
1432 730 611 209 190 188 187 128
121 102 96 93 85 81 79 74
62 60 58 53 51 32 28 26
25 21 16
BspEI T'CCGG_A
Cuts at: 4058 4058
Size: 4838
BspHI T'CATG_A
Cuts at: 4014 4014
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 458 637 1730 1888 2102 2404 2785 458
Size: 179 1093 158 214 302 381 2511
Fragments arranged by size:
2511 1093 381 302 214 179 158
BsrBI CCG'CTC
Cuts at: 50 712 796 1815 3130 3184 4668 4833 50
Size: 662 84 1019 1315 54 1484 165 55
Fragments arranged by size:
1484 1315 1019 662 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 882 2750 882
Size: 1868 2970
BsrFI r'CCGG_y
Cuts at: 145 356 729 913 1042 1049 1070 2836 3017
Size: 211 373 184 129 7 21 1766 181
Cuts at: 3017 3226 145
Size: 209 1757
Fragments arranged by size:
1766 1757 373 211 209 184 181 129
21 7
BssHII G'CGCG_C
Cuts at: 131 131
Size: 4838
BssSI C'ACGA_G
Cuts at: 1026 1303 1642 3109 3467 1026
Size: 277 339 1467 358 2397
Fragments arranged by size:
2397 1467 358 339 277
BstAPI GCAn_nnn'nTGC
Cuts at: 194 1803 2215 194
Size: 1609 412 2817
Fragments arranged by size:
2817 1609 412
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstYI r'GATC_y
Cuts at: 1 257 1863 2330 2353 2688 2934 3946 4032
Size: 256 1606 467 23 335 246 1012 86
Cuts at: 4032 4044 4390 1
Size: 12 346 449
Fragments arranged by size:
1606 1012 467 449 346 335 256 246
86 23 12
BtgI C'CryG_G
Cuts at: 659 705 746 1202 1634 659
Size: 46 41 456 432 3863
Fragments arranged by size:
3863 456 432 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1659 2788 2969 3132 1659
Size: 1129 181 163 3365
Fragments arranged by size:
3365 1129 181 163
BtsI GCAGTG_nn'
Cuts at: 583 1155 2081 583
Size: 572 926 3340
Fragments arranged by size:
3340 926 572
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DraIII CAC_nnn'GTG
Cuts at: 1141 1141
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3402 2544
Size: 858 3980
EaeI y'GGCC_r
Cuts at: 143 351 1058 1631 2423 2597 2988 3015 4360
Size: 208 707 573 792 174 391 27 1345
Cuts at: 4360 143
Size: 621
Fragments arranged by size:
1345 792 707 621 573 391 208 174
27
EagI C'GGCC_G
Cuts at: 1058 1631 2423 1058
Size: 573 792 3473
Fragments arranged by size:
3473 792 573
EarI CTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 732 3046 3356 3502 4648 732
Size: 2314 310 146 1146 922
Fragments arranged by size:
2314 1146 922 310 146
EcoICRI GAG'CTC
Cuts at: 1850 1850
Size: 4838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1200 1412 2663 3095 3842 4110 1120
Size: 80 212 1251 432 747 268 1848
Fragments arranged by size:
1848 1251 747 432 268 212 80
EcoO109I rG'GnC_Cy
Cuts at: 117 229 416 1977 117
Size: 112 187 1561 2978
Fragments arranged by size:
2978 1561 187 112
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FseI GG_CCGG'CC
Cuts at: 149 149
Size: 4838
FspI TGC'GCA
Cuts at: 1726 2619 1726
Size: 893 3945
HaeII r_GCGC'y
Cuts at: 363 411 659 727 826 833 920 942 1125
Size: 48 248 68 99 7 87 22 183
Cuts at: 1125 1702 1711 1742 2272 2520 3542 4521 363
Size: 577 9 31 530 248 1022 979 680
Fragments arranged by size:
1022 979 680 577 530 248 248 183
99 87 68 48 31 22 9 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 1329 1361 1534 1564 1566 1596 2680
Size: 32 372 32 173 30 2 30 1084
Cuts at: 2680 2710 2712 2742 925
Size: 30 2 30 3021
Fragments arranged by size:
3021 1084 372 173 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 1131 1877 1131
Size: 746 4092
KasI G'GCGC_C
Cuts at: 359 655 822 916 1738 2268 2516 359
Size: 296 167 94 822 530 248 2681
Fragments arranged by size:
2681 822 530 296 248 167 94
KpnI G_GTAC'C
Cuts at: 1490 1858 1490
Size: 368 4470
MluI A'CGCG_T
Cuts at: 869 4271 869
Size: 3402 1436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3509 3693 3509
Size: 184 4654
MscI TGG'CCA
Cuts at: 2599 2599
Size: 4838
MslI CAynn'nnrTG
Cuts at: 2827 2954 2827
Size: 127 4711
MspA1I CmG'CkG
Cuts at: 381 532 661 707 748 899 1577 1949 2263
Size: 151 129 46 41 151 678 372 314
Cuts at: 2263 2623 3636 3881 381
Size: 360 1013 245 1338
Fragments arranged by size:
1338 1013 678 372 360 314 245 151
151 129 46 41
NaeI GCC'GGC
Cuts at: 147 358 915 3019 147
Size: 211 557 2104 1966
Fragments arranged by size:
2104 1966 557 211
NarI GG'CG_CC
Cuts at: 360 656 823 917 1739 2269 2517 360
Size: 296 167 94 822 530 248 2681
Fragments arranged by size:
2681 822 530 296 248 167 94
NgoMIV G'CCGG_C
Cuts at: 145 356 913 3017 145
Size: 211 557 2104 1966
Fragments arranged by size:
2104 1966 557 211
NheI G'CTAG_C
Cuts at: 1375 1375
Size: 4838
NruI TCG'CGA
Cuts at: 1678 1678
Size: 4838
NspI r_CATG'y
Cuts at: 1035 1891 2391 3298 1035
Size: 856 500 907 2575
Fragments arranged by size:
2575 907 856 500
PasI CC'CwG_GG
Cuts at: 886 886
Size: 4838
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4838
PfoI T'CCnGG_A
Cuts at: 1403 1403
Size: 4838
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PmlI CAC'GTG
Cuts at: 1803 1803
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 117 117
Size: 4838
PspOMI G'GGCC_C
Cuts at: 228 415 1507 228
Size: 187 1092 3559
Fragments arranged by size:
3559 1092 187
PstI C_TGCA'G
Cuts at: 380 634 844 929 1799 1885 2570 380
Size: 254 210 85 870 86 685 2648
Fragments arranged by size:
2648 870 685 254 210 86 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 381 2263 2623 381
Size: 1882 360 2596
Fragments arranged by size:
2596 1882 360
RsrII CG'GwC_CG
Cuts at: 1808 3033 1808
Size: 1225 3613
SacI G_AGCT'C
Cuts at: 1852 1852
Size: 4838
SacII CC_GC'GG
Cuts at: 662 708 749 662
Size: 46 41 4751
Fragments arranged by size:
4751 46 41
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 2861 3071 2861
Size: 210 4628
SbfI CC_TGCA'GG
Cuts at: 634 929 1885 634
Size: 295 956 3587
Fragments arranged by size:
3587 956 295
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SexAI A'CCwGG_T
Cuts at: 1175 1175
Size: 4838
SfcI C'TryA_G
Cuts at: 33 376 462 630 840 925 1795 1881 2566
Size: 343 86 168 210 85 870 86 685
Cuts at: 2566 3559 3750 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 870 685 343 210 191 168
86 86 85
SfoI GGC'GCC
Cuts at: 361 657 824 918 1740 2270 2518 361
Size: 296 167 94 822 530 248 2681
Fragments arranged by size:
2681 822 530 296 248 167 94
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SgrAI Cr'CCGG_yG
Cuts at: 356 1070 356
Size: 714 4124
SmaI CCC'GGG
Cuts at: 227 1860 227
Size: 1633 3205
SmlI C'TyrA_G
Cuts at: 1993 2908 3400 3662 1993
Size: 915 492 262 3169
Fragments arranged by size:
3169 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StyI C'CwwG_G
Cuts at: 126 1781 1972 2326 126
Size: 1655 191 354 2638
Fragments arranged by size:
2638 1655 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1384 1825 2421 2554 3013 3222 1384
Size: 441 596 133 459 209 3000
Fragments arranged by size:
3000 596 459 441 209 133
TatI w'GTAC_w
Cuts at: 1760 4353 1760
Size: 2593 2245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 562 594 1174 1206 1608 1640 1750 1782 4355
Size: 32 580 32 402 32 110 32 2573
Cuts at: 4355 4387 562
Size: 32 1013
Fragments arranged by size:
2573 1013 580 402 110 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 963 2635 963
Size: 1672 3166
XbaI T'CTAG_A
Cuts at: 1869 1869
Size: 4838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1752 2333 1752
Size: 581 4257
XmaI C'CCGG_G
Cuts at: 225 1858 225
Size: 1633 3205
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NruI NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI AscI AvrII BaeI
BbsI BfrBI BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI
NcoI NdeI NotI NsiI PacI PflMI PshAI PsiI
PspXI PsrI SanDI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI