Restriction map for
pF1KB5606
(Circular) (Six-base) MAPSORT of: pF1KB5606.seq Check: 9762 from: 1 to: 4949
pF1KB5606 4949 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 768 1338 1618 768
Size: 570 280 4099
Fragments arranged by size:
4099 570 280
AccI GT'mk_AC
Cuts at: 217 640 1987 217
Size: 423 1347 3179
Fragments arranged by size:
3179 1347 423
Acc65I G'GTAC_C
Cuts at: 1965 1965
Size: 4949
AclI AA'CG_TT
Cuts at: 4789 4789
Size: 4949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1859 2774 3206 3559 4221 934
Size: 925 915 432 353 662 1662
Fragments arranged by size:
1662 925 915 662 432 353
AflIII A'CryG_T
Cuts at: 2301 4101 4382 2301
Size: 1800 281 2868
Fragments arranged by size:
2868 1800 281
AleI CACnn'nnGTG
Cuts at: 1612 1612
Size: 4949
AlwNI CAG_nnn'CTG
Cuts at: 877 1153 1183 2254 3692 877
Size: 276 30 1071 1438 2134
Fragments arranged by size:
2134 1438 1071 276 30
ApaI G_GGCC'C
Cuts at: 851 851
Size: 4949
ApaLI G'TGCA_C
Cuts at: 3787 3787
Size: 4949
ApoI r'AATT_y
Cuts at: 833 1699 1953 4302 4558 833
Size: 866 254 2349 256 1224
Fragments arranged by size:
2349 1224 866 256 254
AseI AT'TA_AT
Cuts at: 20 1517 20
Size: 1497 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 1746 1969 1746
Size: 223 4726
BamHI G'GATC_C
Cuts at: 1640 1974 1640
Size: 334 4615
BanI G'GyrC_C
Cuts at: 1965 2379 2627 2662 1965
Size: 414 248 35 4252
Fragments arranged by size:
4252 414 248 35
BanII G_rGCy'C
Cuts at: 152 541 851 1508 1670 1963 2993 152
Size: 389 310 657 162 293 1030 2108
Fragments arranged by size:
2108 1030 657 389 310 293 162
BbeI G_GCGC'C
Cuts at: 2383 2631 2383
Size: 248 4701
BbsI GAAGACnn'nnnn_
Cuts at: 1171 1407 1171
Size: 236 4713
BbvCI CC'TCA_GC
Cuts at: 716 716
Size: 4949
BciVI GTATCCnnnnn_n'
Cuts at: 1154 2847 3903 4949 1154
Size: 1693 1056 1046 1154
Fragments arranged by size:
1693 1154 1056 1046
BclI T'GATC_A
Cuts at: 2469 2469
Size: 4949
BglII A'GATC_T
Cuts at: 2464 4501 2464
Size: 2037 2912
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 348 851 1877 2574 2667 3791 348
Size: 503 1026 697 93 1124 1506
Fragments arranged by size:
1506 1124 1026 697 503 93
BmrI ACTGGGnnnn_n'
Cuts at: 571 1249 2161 2242 2577 4721 571
Size: 678 912 81 335 2144 799
Fragments arranged by size:
2144 912 799 678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 465 489 525 3316 465
Size: 24 36 2791 2098
Fragments arranged by size:
2791 2098 36 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 435 548 1133 1546 311
Size: 124 113 585 413 3714
Fragments arranged by size:
3714 585 413 124 113
Bpu10I CC'TnA_GC
Cuts at: 716 843 716
Size: 127 4822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 520 1306 2125 3004 3718 4016 457
Size: 63 786 819 879 714 298 1390
Fragments arranged by size:
1390 879 819 786 714 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 172 1834 4653 172
Size: 1662 2819 468
Fragments arranged by size:
2819 1662 468
BsaAI yAC'GTr
Cuts at: 1422 2302 1422
Size: 880 4069
BsaBI GATnn'nnATC
Cuts at: 6 2463 4500 6
Size: 2457 2037 455
Fragments arranged by size:
2457 2037 455
BsaHI Gr'CG_yC
Cuts at: 2380 2628 3330 4826 2380
Size: 248 702 1496 2503
Fragments arranged by size:
2503 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2138 2659 3337 3748 3895 4169 2138
Size: 521 678 411 147 274 2918
Fragments arranged by size:
2918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 543 573 949 979 1358 1388 543
Size: 30 376 30 379 30 4104
Fragments arranged by size:
4104 379 376 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 365 454 551 1097 1771 365
Size: 89 97 546 674 3543
Fragments arranged by size:
3543 674 546 97 89
BseYI C'CCAG_C
Cuts at: 202 710 726 791 796 2374 3132 3797 4205
Size: 508 16 65 5 1578 758 665 408
Cuts at: 4205 202
Size: 946
Fragments arranged by size:
1578 946 758 665 508 408 65 16
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 525 644 796 152
Size: 373 119 152 4305
Fragments arranged by size:
4305 373 152 119
BsiEI CG_ry'CG
Cuts at: 104 2491 2537 3767 104
Size: 2387 46 1230 1286
Fragments arranged by size:
2387 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 541 1292 1359 1936 1963 2741 2931 3791 541
Size: 751 67 577 27 778 190 860 1699
Fragments arranged by size:
1699 860 778 751 577 190 67 27
Bsp1286I G_dGCh'C
Cuts at: 152 348 541 851 1292 1359 1508 1670 1877
Size: 196 193 310 441 67 149 162 207
Cuts at: 1877 1936 1963 2574 2667 2741 2931 2993 3791
Size: 59 27 611 93 74 190 62 798
Cuts at: 3791 152
Size: 1310
Fragments arranged by size:
1310 798 611 441 310 207 196 193
190 162 149 93 74 67 62 59
27
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 768 1338 1444 1618 1900 1999 2213 2515 2896
Size: 570 106 174 282 99 214 302 381
Cuts at: 2896 768
Size: 2821
Fragments arranged by size:
2821 570 381 302 282 214 174 106
99
BsrBI CCG'CTC
Cuts at: 50 1563 3241 3295 4779 4944 50
Size: 1513 1678 54 1484 165 55
Fragments arranged by size:
1678 1513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1207 1542 2861 1207
Size: 335 1319 3295
Fragments arranged by size:
3295 1319 335
BsrFI r'CCGG_y
Cuts at: 2947 3128 2947
Size: 181 4768
BssSI C'ACGA_G
Cuts at: 3220 3928 3220
Size: 708 4241
BstAPI GCAn_nnn'nTGC
Cuts at: 1445 1892 2326 1445
Size: 447 434 4068
Fragments arranged by size:
4068 447 434
BstBI TT'CG_AA
Cuts at: 3310 3310
Size: 4949
BstEII G'GTnAC_C
Cuts at: 1069 1069
Size: 4949
BstXI CCAn_nnnn'nTGG
Cuts at: 193 489 684 1082 193
Size: 296 195 398 4060
Fragments arranged by size:
4060 398 296 195
BstYI r'GATC_y
Cuts at: 1 1179 1640 1974 2441 2464 2799 3045 3351
Size: 1178 461 334 467 23 335 246 306
Cuts at: 3351 3363 3449 4501 1
Size: 12 86 1052 449
Fragments arranged by size:
1178 1052 467 461 449 335 334 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 740 1403 1708 740
Size: 663 305 3981
Fragments arranged by size:
3981 663 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2899 3080 3243 2899
Size: 181 163 4605
Fragments arranged by size:
4605 181 163
BtsI GCAGTG_nn'
Cuts at: 895 1397 1537 1674 1800 2192 895
Size: 502 140 137 126 392 3652
Fragments arranged by size:
3652 502 392 140 137 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1408 1443 1408
Size: 35 4914
DraI TTT'AAA
Cuts at: 1948 1948
Size: 4949
DrdI GACnn_nn'nnGTC
Cuts at: 2655 3999 2655
Size: 1344 3605
EaeI y'GGCC_r
Cuts at: 156 1224 1347 2534 2708 3099 3126 4471 156
Size: 1068 123 1187 174 391 27 1345 634
Fragments arranged by size:
1345 1187 1068 634 391 174 123 27
EagI C'GGCC_G
Cuts at: 2534 2534
Size: 4949
EarI CTCTTCn'nnn_
Cuts at: 389 2972 3182 389
Size: 2583 210 2156
Fragments arranged by size:
2583 2156 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1258 3157 3899 4045 4759 1258
Size: 1899 742 146 714 1448
Fragments arranged by size:
1899 1448 742 714 146
EcoICRI GAG'CTC
Cuts at: 539 1961 539
Size: 1422 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 435 548 934 1133 1546 1859 2774 3206
Size: 124 113 386 199 413 313 915 432
Cuts at: 3206 3559 4221 311
Size: 353 662 1039
Fragments arranged by size:
1039 915 662 432 413 386 353 313
199 124 113
EcoNI CCTnn'n_nnAGG
Cuts at: 824 1115 824
Size: 291 4658
EcoO109I rG'GnC_Cy
Cuts at: 847 848 1243 2088 847
Size: 1 395 845 3708
Fragments arranged by size:
3708 845 395 1
EcoRI G'AATT_C
Cuts at: 1953 1953
Size: 4949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4949
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 450 2383 2631 3861 4632 450
Size: 1933 248 1230 771 767
Fragments arranged by size:
1933 1230 771 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2791 2821 2823 2853 2791
Size: 30 2 30 4887
Fragments arranged by size:
4887 30 30 2
HincII GTy'rAC
Cuts at: 1063 1919 1988 1063
Size: 856 69 4024
Fragments arranged by size:
4024 856 69
KasI G'GCGC_C
Cuts at: 2379 2627 2379
Size: 248 4701
KpnI G_GTAC'C
Cuts at: 1969 1969
Size: 4949
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3708 3892 3708
Size: 184 4765
MscI TGG'CCA
Cuts at: 1226 2710 1226
Size: 1484 3465
MslI CAynn'nnrTG
Cuts at: 211 682 1402 1612 1912 2938 3065 211
Size: 471 720 210 300 1026 127 2095
Fragments arranged by size:
2095 1026 720 471 300 210 127
MspA1I CmG'CkG
Cuts at: 1352 1710 2060 2374 2734 3518 3763 1352
Size: 358 350 314 360 784 245 2538
Fragments arranged by size:
2538 784 360 358 350 314 245
NaeI GCC'GGC
Cuts at: 3130 3130
Size: 4949
NarI GG'CG_CC
Cuts at: 2380 2628 2380
Size: 248 4701
NcoI C'CATG_G
Cuts at: 1403 1403
Size: 4949
NgoMIV G'CCGG_C
Cuts at: 3128 3128
Size: 4949
NspI r_CATG'y
Cuts at: 2002 2502 4105 2002
Size: 500 1603 2846
Fragments arranged by size:
2846 1603 500
PasI CC'CwG_GG
Cuts at: 852 852
Size: 4949
PciI A'CATG_T
Cuts at: 4101 4101
Size: 4949
PflMI CCAn_nnn'nTGG
Cuts at: 1027 1153 1222 1027
Size: 126 69 4754
Fragments arranged by size:
4754 126 69
PfoI T'CCnGG_A
Cuts at: 420 1110 420
Size: 690 4259
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PmlI CAC'GTG
Cuts at: 1422 1422
Size: 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4917
PshAI GACnn'nnGTC
Cuts at: 577 577
Size: 4949
PspOMI G'GGCC_C
Cuts at: 847 847
Size: 4949
PstI C_TGCA'G
Cuts at: 1996 2681 1996
Size: 685 4264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 2374 2734 2374
Size: 360 4589
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 541 1963 541
Size: 1422 3527
SacII CC_GC'GG
Cuts at: 1711 1711
Size: 4949
SalI G'TCGA_C
Cuts at: 1986 1986
Size: 4949
SapI GCTCTTCn'nnn_
Cuts at: 2972 3182 2972
Size: 210 4739
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 4466 4466
Size: 4949
SfcI C'TryA_G
Cuts at: 33 1992 2677 3645 3836 33
Size: 1959 685 968 191 1146
Fragments arranged by size:
1959 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2381 2629 2381
Size: 248 4701
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1748 1971 1748
Size: 223 4726
SmlI C'TyrA_G
Cuts at: 436 499 1285 2104 3019 3733 3995 436
Size: 63 786 819 915 714 262 1390
Fragments arranged by size:
1390 915 819 786 714 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 2002 2502 2002
Size: 500 4449
StuI AGG'CCT
Cuts at: 762 1588 762
Size: 826 4123
StyI C'CwwG_G
Cuts at: 1403 1851 1865 1925 2083 2437 1403
Size: 448 14 60 158 354 3915
Fragments arranged by size:
3915 448 354 158 60 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 147 199 246 1088 2532 2665 3124 3333
Size: 16 52 47 842 1444 133 459 209
Cuts at: 3333 131
Size: 1747
Fragments arranged by size:
1747 1444 842 459 209 133 52 47
16
TatI w'GTAC_w
Cuts at: 269 408 4464 269
Size: 139 4056 754
Fragments arranged by size:
4056 754 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 4917
Tth111I GACn'n_nGTC
Cuts at: 2746 2746
Size: 4949
XbaI T'CTAG_A
Cuts at: 330 1980 330
Size: 1650 3299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1132 1554 2444 1132
Size: 422 890 3637
Fragments arranged by size:
3637 890 422
XmaI C'CCGG_G
Cuts at: 1746 1969 1746
Size: 223 4726
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PpuMI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI