Restriction map for
pF1KB5610
(Circular) (Six-base) MAPSORT of: pF1KB5610.seq Check: 7815 from: 1 to: 6149
pF1KB5610 6149 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 1851 3187 1851
Size: 1336 4813
Acc65I G'GTAC_C
Cuts at: 3165 3165
Size: 6149
AclI AA'CG_TT
Cuts at: 5989 5989
Size: 6149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1040 2414 2464 2543 2869 3974 4406 5153 5421
Size: 1374 50 79 326 1105 432 747 268
Cuts at: 5421 1040
Size: 1768
Fragments arranged by size:
1768 1374 1105 747 432 326 268 79
50
AflIII A'CryG_T
Cuts at: 869 3501 4605 5582 869
Size: 2632 1104 977 1436
Fragments arranged by size:
2632 1436 1104 977
AgeI A'CCGG_T
Cuts at: 4537 4537
Size: 6149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 923 1292 2485 923
Size: 369 1193 4587
Fragments arranged by size:
4587 1193 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 859 891 859
Size: 32 6117
AlwNI CAG_nnn'CTG
Cuts at: 1795 3142 3454 5021 1795
Size: 1347 312 1567 2923
Fragments arranged by size:
2923 1567 1347 312
ApaLI G'TGCA_C
Cuts at: 2191 4919 2191
Size: 2728 3421
ApoI r'AATT_y
Cuts at: 409 457 509 1729 2224 3153 5502 5758 409
Size: 48 52 1220 495 929 2349 256 800
Fragments arranged by size:
2349 1220 929 800 495 256 52 48
AseI AT'TA_AT
Cuts at: 20 944 20
Size: 924 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6149
AvaI C'yCGr_G
Cuts at: 168 238 2331 3169 168
Size: 70 2093 838 3148
Fragments arranged by size:
3148 2093 838 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2204 2237 2204
Size: 33 6116
BamHI G'GATC_C
Cuts at: 3174 3174
Size: 6149
BanI G'GyrC_C
Cuts at: 395 1999 3165 3579 3827 3862 395
Size: 1604 1166 414 248 35 2682
Fragments arranged by size:
2682 1604 1166 414 248 35
BanII G_rGCy'C
Cuts at: 318 2018 3163 4193 318
Size: 1700 1145 1030 2274
Fragments arranged by size:
2274 1700 1145 1030
BbeI G_GCGC'C
Cuts at: 3583 3831 3583
Size: 248 5901
BbsI GAAGACnn'nnnn_
Cuts at: 281 374 1889 2170 281
Size: 93 1515 281 4260
Fragments arranged by size:
4260 1515 281 93
BciVI GTATCCnnnnn_n'
Cuts at: 2205 4047 4808 6149 2205
Size: 1842 761 1341 2205
Fragments arranged by size:
2205 1842 1341 761
BclI T'GATC_A
Cuts at: 1926 2340 2987 3669 1926
Size: 414 647 682 4406
Fragments arranged by size:
4406 682 647 414
BglII A'GATC_T
Cuts at: 1134 1937 2591 3664 5701 1134
Size: 803 654 1073 2037 1582
Fragments arranged by size:
2037 1582 1073 803 654
BlpI GC'TnA_GC
Cuts at: 2012 3261 2012
Size: 1249 4900
Bme1580I G_kGCm'C
Cuts at: 281 525 2195 3774 3867 4923 281
Size: 244 1670 1579 93 1056 1507
Fragments arranged by size:
1670 1579 1507 1056 244 93
BmgBI CAC'GTC
Cuts at: 210 631 210
Size: 421 5728
BmrI ACTGGGnnnn_n'
Cuts at: 2266 2376 3361 3442 3777 5921 2266
Size: 110 985 81 335 2144 2494
Fragments arranged by size:
2494 2144 985 335 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1000 1759 4516 1000
Size: 759 2757 2633
Fragments arranged by size:
2757 2633 759
Bpu10I CC'TnA_GC
Cuts at: 2076 2076
Size: 6149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 3065 3325 4204 4696 4994 1579
Size: 1486 260 879 492 298 2734
Fragments arranged by size:
2734 1486 879 492 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 1038 5853 1038
Size: 4815 1334
BsaAI yAC'GTr
Cuts at: 2784 3502 2784
Size: 718 5431
BsaBI GATnn'nnATC
Cuts at: 6 1925 3663 5700 6
Size: 1919 1738 2037 455
Fragments arranged by size:
2037 1919 1738 455
BsaHI Gr'CG_yC
Cuts at: 3580 3828 4530 6026 3580
Size: 248 702 1496 3703
Fragments arranged by size:
3703 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3338 3859 4537 4811 4958 5369 3338
Size: 521 678 274 147 411 4118
Fragments arranged by size:
4118 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 347 377 473 503 859 889 347
Size: 30 96 30 356 30 5607
Fragments arranged by size:
5607 356 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2339 2339
Size: 6149
BseYI C'CCAG_C
Cuts at: 280 335 3574 4332 4909 5405 280
Size: 55 3239 758 577 496 1024
Fragments arranged by size:
3239 1024 758 577 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 2538 710
Size: 1828 4321
BsiEI CG_ry'CG
Cuts at: 104 3691 3737 4945 104
Size: 3587 46 1208 1308
Fragments arranged by size:
3587 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 318 981 1623 2018 2195 2375 3163 3941 4131
Size: 663 642 395 177 180 788 778 190
Cuts at: 4131 4923 318
Size: 792 1544
Fragments arranged by size:
1544 792 788 778 663 642 395 190
180 177
BsmI GAATG_Cn'
Cuts at: 2951 2951
Size: 6149
Bsp1286I G_dGCh'C
Cuts at: 281 318 525 981 1623 2018 2195 2375 3163
Size: 37 207 456 642 395 177 180 788
Cuts at: 3163 3774 3867 3941 4131 4193 4923 281
Size: 611 93 74 190 62 730 1507
Fragments arranged by size:
1507 788 730 642 611 456 395 207
190 180 177 93 74 62 37
BspEI T'CCGG_A
Cuts at: 5369 5369
Size: 6149
BspHI T'CATG_A
Cuts at: 3114 5325 3114
Size: 2211 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 351 1020 1477 1628 3199 3413 3715 4096 351
Size: 669 457 151 1571 214 302 381 2404
Fragments arranged by size:
2404 1571 669 457 381 302 214 151
BsrBI CCG'CTC
Cuts at: 50 2646 4441 4495 5979 6144 50
Size: 2596 1795 54 1484 165 55
Fragments arranged by size:
2596 1795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2474 4061 2474
Size: 1587 4562
BsrFI r'CCGG_y
Cuts at: 392 4147 4328 4537 392
Size: 3755 181 209 2004
Fragments arranged by size:
3755 2004 209 181
BsrGI T'GTAC_A
Cuts at: 3044 3044
Size: 6149
BssSI C'ACGA_G
Cuts at: 307 4420 4778 307
Size: 4113 358 1678
Fragments arranged by size:
4113 1678 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 3526 1171
Size: 2355 3794
BstBI TT'CG_AA
Cuts at: 4510 4510
Size: 6149
BstEII G'GTnAC_C
Cuts at: 2928 2928
Size: 6149
BstYI r'GATC_y
Cuts at: 1 1134 1937 2591 2670 3174 3641 3664 3999
Size: 1133 803 654 79 504 467 23 335
Cuts at: 3999 4245 5257 5343 5355 5701 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1133 1012 803 654 504 467 449 346
335 246 86 79 23 12
BtgI C'CryG_G
Cuts at: 107 121 147 263 107
Size: 14 26 116 5993
Fragments arranged by size:
5993 116 26 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4099 4280 4443 4099
Size: 181 163 5805
Fragments arranged by size:
5805 181 163
BtsI GCAGTG_nn'
Cuts at: 1119 1276 1474 1696 2032 3392 1119
Size: 157 198 222 336 1360 3876
Fragments arranged by size:
3876 1360 336 222 198 157
DraI TTT'AAA
Cuts at: 1451 3063 3148 1451
Size: 1612 85 4452
Fragments arranged by size:
4452 1612 85
DrdI GACnn_nn'nnGTC
Cuts at: 3855 4713 3855
Size: 858 5291
EaeI y'GGCC_r
Cuts at: 3073 3734 3908 4299 4326 5671 3073
Size: 661 174 391 27 1345 3551
Fragments arranged by size:
3551 1345 661 391 174 27
EagI C'GGCC_G
Cuts at: 3734 3734
Size: 6149
EarI CTCTTCn'nnn_
Cuts at: 991 4172 4382 991
Size: 3181 210 2758
Fragments arranged by size:
3181 2758 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 107 4357 4667 4813 5959 107
Size: 4250 310 146 1146 297
Fragments arranged by size:
4250 1146 310 297 146
EcoICRI GAG'CTC
Cuts at: 316 2016 3161 316
Size: 1700 1145 3304
Fragments arranged by size:
3304 1700 1145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1040 2414 2464 2543 2869 3974 4406 5153 5421
Size: 1374 50 79 326 1105 432 747 268
Cuts at: 5421 1040
Size: 1768
Fragments arranged by size:
1768 1374 1105 747 432 326 268 79
50
EcoO109I rG'GnC_Cy
Cuts at: 363 1640 3288 363
Size: 1277 1648 3224
Fragments arranged by size:
3224 1648 1277
EcoRI G'AATT_C
Cuts at: 3153 3153
Size: 6149
EcoRV GAT'ATC
Cuts at: 1345 2910 1345
Size: 1565 4584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1564 2311 4168 1564
Size: 747 1857 3545
Fragments arranged by size:
3545 1857 747
FspI TGC'GCA
Cuts at: 3930 3930
Size: 6149
HaeII r_GCGC'y
Cuts at: 2965 3583 3831 4853 5832 2965
Size: 618 248 1022 979 3282
Fragments arranged by size:
3282 1022 979 618 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 985 1017 1079 1111 1394 1426 1695
Size: 32 606 32 62 32 283 32 269
Cuts at: 1695 1727 2325 2357 3991 4021 4023 4053 347
Size: 32 598 32 1634 30 2 30 2443
Fragments arranged by size:
2443 1634 606 598 283 269 62 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1852 3188 1852
Size: 1336 4813
HindIII A'AGCT_T
Cuts at: 1296 1296
Size: 6149
KasI G'GCGC_C
Cuts at: 3579 3827 3579
Size: 248 5901
KpnI G_GTAC'C
Cuts at: 3169 3169
Size: 6149
MfeI C'AATT_G
Cuts at: 404 1166 2900 404
Size: 762 1734 3653
Fragments arranged by size:
3653 1734 762
MluI A'CGCG_T
Cuts at: 5582 5582
Size: 6149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 552 753 1757 4820 5004 552
Size: 201 1004 3063 184 1697
Fragments arranged by size:
3063 1697 1004 201 184
MscI TGG'CCA
Cuts at: 3910 3910
Size: 6149
MslI CAynn'nnrTG
Cuts at: 2426 2796 2971 3119 4138 4265 2426
Size: 370 175 148 1019 127 4310
Fragments arranged by size:
4310 1019 370 175 148 127
MspA1I CmG'CkG
Cuts at: 123 149 1792 3260 3574 3934 4947 5192 123
Size: 26 1643 1468 314 360 1013 245 1080
Fragments arranged by size:
1643 1468 1080 1013 360 314 245 26
NaeI GCC'GGC
Cuts at: 4330 4330
Size: 6149
NarI GG'CG_CC
Cuts at: 3580 3828 3580
Size: 248 5901
NcoI C'CATG_G
Cuts at: 107 107
Size: 6149
NdeI CA'TA_TG
Cuts at: 1179 1179
Size: 6149
NgoMIV G'CCGG_C
Cuts at: 4328 4328
Size: 6149
NspI r_CATG'y
Cuts at: 581 873 1290 3202 3702 4609 581
Size: 292 417 1912 500 907 2121
Fragments arranged by size:
2121 1912 907 500 417 292
PciI A'CATG_T
Cuts at: 869 4605 869
Size: 3736 2413
PflMI CCAn_nnn'nTGG
Cuts at: 1023 1300 1023
Size: 277 5872
PfoI T'CCnGG_A
Cuts at: 292 292
Size: 6149
PmeI GTTT'AAAC
Cuts at: 3148 3148
Size: 6149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 859 891 5288 5320 859
Size: 32 4397 32 1688
Fragments arranged by size:
4397 1688 32 32
PpuMI rG'GwC_Cy
Cuts at: 363 1640 363
Size: 1277 4872
PsiI TTA'TAA
Cuts at: 2888 2888
Size: 6149
PstI C_TGCA'G
Cuts at: 2039 2313 3196 3881 2039
Size: 274 883 685 4307
Fragments arranged by size:
4307 883 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6149
PvuII CAG'CTG
Cuts at: 1792 3574 3934 1792
Size: 1782 360 4007
Fragments arranged by size:
4007 1782 360
RsrII CG'GwC_CG
Cuts at: 4344 4344
Size: 6149
SacI G_AGCT'C
Cuts at: 318 2018 3163 318
Size: 1700 1145 3304
Fragments arranged by size:
3304 1700 1145
SacII CC_GC'GG
Cuts at: 124 150 124
Size: 26 6123
SalI G'TCGA_C
Cuts at: 1850 3186 1850
Size: 1336 4813
SapI GCTCTTCn'nnn_
Cuts at: 4172 4382 4172
Size: 210 5939
SbfI CC_TGCA'GG
Cuts at: 3196 3196
Size: 6149
ScaI AGT'ACT
Cuts at: 229 1547 5666 229
Size: 1318 4119 712
Fragments arranged by size:
4119 1318 712
SfcI C'TryA_G
Cuts at: 33 926 1045 1632 1916 2035 2309 3192 3877
Size: 893 119 587 284 119 274 883 685
Cuts at: 3877 4870 5061 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 893 883 685 587 284 274
191 119 119
SfoI GGC'GCC
Cuts at: 3581 3829 3581
Size: 248 5901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6149
SgrAI Cr'CCGG_yG
Cuts at: 392 392
Size: 6149
SmaI CCC'GGG
Cuts at: 3171 3171
Size: 6149
SmlI C'TyrA_G
Cuts at: 1594 2331 3080 3304 4219 4711 4973 1594
Size: 737 749 224 915 492 262 2770
Fragments arranged by size:
2770 915 749 737 492 262 224
SnaBI TAC'GTA
Cuts at: 2784 2784
Size: 6149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6149
SphI G_CATG'C
Cuts at: 1290 3202 3702 1290
Size: 1912 500 3737
Fragments arranged by size:
3737 1912 500
StyI C'CwwG_G
Cuts at: 107 440 2136 3283 3637 107
Size: 333 1696 1147 354 2619
Fragments arranged by size:
2619 1696 1147 354 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 349 2973 3732 3865 4324 4533 130
Size: 219 2624 759 133 459 209 1746
Fragments arranged by size:
2624 1746 759 459 219 209 133
TatI w'GTAC_w
Cuts at: 227 1545 3044 5664 227
Size: 1318 1499 2620 712
Fragments arranged by size:
2620 1499 1318 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 473 505 5666 5698 473
Size: 32 5161 32 924
Fragments arranged by size:
5161 924 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 3946 1888
Size: 2058 4091
XbaI T'CTAG_A
Cuts at: 3180 3180
Size: 6149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 475 533 1125 2922 3644 475
Size: 58 592 1797 722 2980
Fragments arranged by size:
2980 1797 722 592 58
XhoI C'TCGA_G
Cuts at: 2331 2331
Size: 6149
XmaI C'CCGG_G
Cuts at: 3169 3169
Size: 6149
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI BpmI BsiWI
BsmBI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HpaI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SanDI
SexAI SfiI SrfI SspI StuI SwaI XmnI ZraI