Restriction map for
pF1KB5611
(Circular) (Six-base) MAPSORT of: pF1KB5611.seq Check: 7983 from: 1 to: 4259
pF1KB5611 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 631 631
Size: 4259
AccI GT'mk_AC
Cuts at: 923 1297 923
Size: 374 3885
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 785 809 2084 2516 3263 3531 785
Size: 24 1275 432 747 268 1513
Fragments arranged by size:
1513 1275 747 432 268 24
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AhdI GACnn_n'nnGTC
Cuts at: 883 883
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 763 1564 3131 763
Size: 801 1567 1891
Fragments arranged by size:
1891 1567 801
ApaI G_GGCC'C
Cuts at: 696 696
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 629 1263 3612 3868 629
Size: 634 2349 256 1020
Fragments arranged by size:
2349 1020 634 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 1279 1279
Size: 4259
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1275 1689 1937 1972 1275
Size: 414 248 35 3562
Fragments arranged by size:
3562 414 248 35
BanII G_rGCy'C
Cuts at: 696 857 1273 2303 696
Size: 161 416 1030 2652
Fragments arranged by size:
2652 1030 416 161
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 798 1779 798
Size: 981 3278
BglII A'GATC_T
Cuts at: 784 1774 3811 784
Size: 990 2037 1232
Fragments arranged by size:
2037 1232 990
BlpI GC'TnA_GC
Cuts at: 184 1371 184
Size: 1187 3072
Bme1580I G_kGCm'C
Cuts at: 696 1884 1977 3033 696
Size: 1188 93 1056 1922
Fragments arranged by size:
1922 1188 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 637 445
Size: 192 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 919 1435 2314 2806 3104 531
Size: 388 516 879 492 298 1686
Fragments arranged by size:
1686 879 516 492 388 298
BsaI GGTCTCn'nnnn_
Cuts at: 3963 3963
Size: 4259
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 970 1773 3810 6
Size: 964 803 2037 455
Fragments arranged by size:
2037 964 803 455
BsaHI Gr'CG_yC
Cuts at: 1085 1690 1938 2640 4136 1085
Size: 605 248 702 1496 1208
Fragments arranged by size:
1496 1208 702 605 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1012 1042 1012
Size: 30 4229
BseYI C'CCAG_C
Cuts at: 1135 1684 2442 3019 3515 1135
Size: 549 758 577 496 1879
Fragments arranged by size:
1879 758 577 549 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 381 857 1273 2051 2241 3033 381
Size: 476 416 778 190 792 1607
Fragments arranged by size:
1607 792 778 476 416 190
BsmI GAATG_Cn'
Cuts at: 642 1051 642
Size: 409 3850
Bsp1286I G_dGCh'C
Cuts at: 381 696 857 1273 1884 1977 2051 2241 2303
Size: 315 161 416 611 93 74 190 62
Cuts at: 2303 3033 381
Size: 730 1607
Fragments arranged by size:
1607 730 611 416 315 190 161 93
74 62
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 1309 1523 1825 2206 631
Size: 678 214 302 381 2684
Fragments arranged by size:
2684 678 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2551 2605 4089 4254 50
Size: 2501 54 1484 165 55
Fragments arranged by size:
2501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 918 2171 918
Size: 1253 3006
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstYI r'GATC_y
Cuts at: 1 784 871 1284 1751 1774 2109 2355 3367
Size: 783 87 413 467 23 335 246 1012
Cuts at: 3367 3453 3465 3811 1
Size: 86 12 346 449
Fragments arranged by size:
1012 783 467 449 413 346 335 246
87 86 23 12
BtgI C'CryG_G
Cuts at: 107 723 107
Size: 616 3643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 1016 1502 1016
Size: 486 3773
DraI TTT'AAA
Cuts at: 363 1258 363
Size: 895 3364
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 1844 2018 2409 2436 3781 1844
Size: 174 391 27 1345 2322
Fragments arranged by size:
2322 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 845 2282 2492 845
Size: 1437 210 2612
Fragments arranged by size:
2612 1437 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 2777 2923 4069 2467
Size: 310 146 1146 2657
Fragments arranged by size:
2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 855 1271 855
Size: 416 3843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 637 785 809 2084 2516 3263 3531 445
Size: 192 148 24 1275 432 747 268 1173
Fragments arranged by size:
1275 1173 747 432 268 192 148 24
EcoO109I rG'GnC_Cy
Cuts at: 692 693 733 1398 692
Size: 1 40 665 3553
Fragments arranged by size:
3553 665 40 1
EcoRI G'AATT_C
Cuts at: 629 1263 629
Size: 634 3625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 1111 2040 1111
Size: 929 3330
FspAI rTGC'GCAy
Cuts at: 1111 1111
Size: 4259
HaeII r_GCGC'y
Cuts at: 1693 1941 2963 3942 1693
Size: 248 1022 979 2010
Fragments arranged by size:
2010 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 869 901 1213 1219 1245 1251 2101 2131 2133
Size: 32 312 6 26 6 850 30 2
Cuts at: 2133 2163 869
Size: 30 2965
Fragments arranged by size:
2965 850 312 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 488 924 1298 488
Size: 436 374 3449
Fragments arranged by size:
3449 436 374
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 2930 3114 1010
Size: 1920 184 2155
Fragments arranged by size:
2155 1920 184
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 961 2248 2375 961
Size: 1287 127 2845
Fragments arranged by size:
2845 1287 127
MspA1I CmG'CkG
Cuts at: 493 615 1370 1684 2044 3057 3302 493
Size: 122 755 314 360 1013 245 1450
Fragments arranged by size:
1450 1013 755 360 314 245 122
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 107 723 107
Size: 616 3643
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 195 295 821 1312 1812 2719 195
Size: 100 526 491 500 907 1735
Fragments arranged by size:
1735 907 526 500 491 100
PasI CC'CwG_GG
Cuts at: 153 153
Size: 4259
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PpuMI rG'GwC_Cy
Cuts at: 733 733
Size: 4259
PspOMI G'GGCC_C
Cuts at: 692 692
Size: 4259
PstI C_TGCA'G
Cuts at: 375 1306 1991 375
Size: 931 685 2643
Fragments arranged by size:
2643 931 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 493 1684 2044 493
Size: 1191 360 2708
Fragments arranged by size:
2708 1191 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 857 1273 857
Size: 416 3843
SalI G'TCGA_C
Cuts at: 922 1296 922
Size: 374 3885
SapI GCTCTTCn'nnn_
Cuts at: 845 2282 2492 845
Size: 1437 210 2612
Fragments arranged by size:
2612 1437 210
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 371 494 1302 1987 2980 3171 33
Size: 338 123 808 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 338 191 123
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 546 898 1414 2329 2821 3083 546
Size: 352 516 915 492 262 1722
Fragments arranged by size:
1722 915 516 492 352 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 195 295 1312 1812 195
Size: 100 1017 500 2642
Fragments arranged by size:
2642 1017 500 100
StyI C'CwwG_G
Cuts at: 107 696 723 1393 1747 107
Size: 589 27 670 354 2619
Fragments arranged by size:
2619 670 589 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1005 1842 1975 2434 2643 1005
Size: 837 133 459 209 2621
Fragments arranged by size:
2621 837 459 209 133
TatI w'GTAC_w
Cuts at: 3774 3774
Size: 4259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 1096 2056 1096
Size: 960 3299
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1754 1754
Size: 4259
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
XmnI GAAnn'nnTTC
Cuts at: 629 629
Size: 4259
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI